ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart29d03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RPOC2_EUCGG (Q49L08) DNA-directed RNA polymerase beta'' chain (E... 30 4.3
2MTF1_SACKL (P87292) Mitochondrial replication protein MTF1 (Mito... 30 5.6
3TIM44_YEAST (Q01852) Import inner membrane translocase subunit T... 30 5.6
4CCNE1_CHICK (P49707) G1/S-specific cyclin-E1 30 5.6
5SYS2_CLOAB (Q97N21) Seryl-tRNA synthetase 2 (EC 6.1.1.11) (Serin... 29 7.3
6TOP2_CAEEL (Q23670) Probable DNA topoisomerase 2 (EC 5.99.1.3) (... 29 9.5
7SRD3_CAEEL (O17240) Serpentine receptor class delta-3 (Protein s... 29 9.5

>RPOC2_EUCGG (Q49L08) DNA-directed RNA polymerase beta'' chain (EC 2.7.7.6)|
           (PEP) (Plastid-encoded RNA polymerase beta'' subunit)
           (RNA polymerase beta'' subunit)
          Length = 1392

 Score = 30.0 bits (66), Expect = 4.3
 Identities = 23/68 (33%), Positives = 32/68 (47%)
 Frame = -2

Query: 264 EMPDTKSSGNAKEYTADRDVKYEELQRFNQADAWNAYVVGVANI**MKSHCNLKYPKDVF 85
           E+  +  SG  KE   DR   Y EL R          ++ +        HCNLKYP   F
Sbjct: 527 ELCSSDFSGKIKE---DRIPDYSELNR----------IISIV-------HCNLKYP-TTF 565

Query: 84  DDSSELLS 61
           D++S+LL+
Sbjct: 566 DENSDLLA 573



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>MTF1_SACKL (P87292) Mitochondrial replication protein MTF1 (Mitochondrial|
           transcription factor mtTFB)
          Length = 337

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 26/92 (28%), Positives = 39/92 (42%), Gaps = 4/92 (4%)
 Frame = -2

Query: 525 FVMDVYGGTSSECKLRLQRRFPSFTYFWEQEEFDIITRETRISLHFQ----VGKKQMIRH 358
           F+ D + GTS E   R    + S+T   E+E+  +  R++R  +H Q         MI  
Sbjct: 83  FIQDNFAGTSMELYQRDPYEWSSYTDMIEKEKRFVPNRQSRDKIHNQFLVMANLTGMIGE 142

Query: 357 AFTYHWRLWSIPEIKDCLEEAGFKSIHVWVRE 262
                W   S    K+ L+  G   + VWV E
Sbjct: 143 GLFMQW--LSCIGNKNWLQRFGRVKMLVWVPE 172



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>TIM44_YEAST (Q01852) Import inner membrane translocase subunit TIM44,|
           mitochondrial precursor (Mitochondrial protein import
           protein 1) (Inner membrane import site protein 45)
           (ISP45) (Membrane import machinery protein MIM44)
          Length = 431

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 14/54 (25%), Positives = 24/54 (44%)
 Frame = -2

Query: 417 TRETRISLHFQVGKKQMIRHAFTYHWRLWSIPEIKDCLEEAGFKSIHVWVREMP 256
           T  +R+   F++        +FT H R + +PEI +   +   K +  W  E P
Sbjct: 268 TESSRVYSQFKLMDPTFSNESFTRHLREYIVPEILEAYVKGDVKVLKKWFSEAP 321



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>CCNE1_CHICK (P49707) G1/S-specific cyclin-E1|
          Length = 407

 Score = 29.6 bits (65), Expect = 5.6
 Identities = 26/97 (26%), Positives = 42/97 (43%), Gaps = 4/97 (4%)
 Frame = +1

Query: 76  TVIENILWILQVAMRFHLLDVCYPDYVRVPSICLVETLELFVLHVAVRGVLLGVSA*FGV 255
           TV+  +   +QVA    L +V  P Y +   + + E L+L VL +       GV A   +
Sbjct: 242 TVVSWLNIYMQVAYLNELYEVLLPQYPQQIFVQIAELLDLCVLDIGCLEYTYGVLAASAL 301

Query: 256 WHLPDPD----VDGLESSFLQAILDFWYRPQAPMVRE 354
           +H    +    V G E   ++  +  W  P A  +RE
Sbjct: 302 YHFSSSELMQKVSGYEWCEIEECVK-WMVPFAMAIRE 337



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>SYS2_CLOAB (Q97N21) Seryl-tRNA synthetase 2 (EC 6.1.1.11) (Serine--tRNA ligase|
           2) (SerRS 2)
          Length = 425

 Score = 29.3 bits (64), Expect = 7.3
 Identities = 21/82 (25%), Positives = 35/82 (42%), Gaps = 6/82 (7%)
 Frame = -2

Query: 384 VGKKQMIRHAFTYHWRLWSIPEIKDCLEEAGFKSIHVWVREMPDTK---SSGNAKEYTAD 214
           +GK + +      H+RL  +         A    I VW+  M + K   S+ NA++Y A 
Sbjct: 306 IGKAEKLVKGLGLHYRLSKLAAADCSASMAKTYDIEVWIPSMNEYKEVSSASNARDYQAR 365

Query: 213 RD---VKYEELQRFNQADAWNA 157
           R     + EE ++    +  NA
Sbjct: 366 RGKIRFRREETKKIEYVNTLNA 387



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>TOP2_CAEEL (Q23670) Probable DNA topoisomerase 2 (EC 5.99.1.3) (DNA|
           topoisomerase II)
          Length = 1520

 Score = 28.9 bits (63), Expect = 9.5
 Identities = 11/31 (35%), Positives = 18/31 (58%)
 Frame = -2

Query: 273 WVREMPDTKSSGNAKEYTADRDVKYEELQRF 181
           W+RE  D K  G A+EY  ++D ++   + F
Sbjct: 703 WMREKKDRKQQGLAEEYLYNKDTRFVTFKDF 733



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>SRD3_CAEEL (O17240) Serpentine receptor class delta-3 (Protein srd-3)|
          Length = 344

 Score = 28.9 bits (63), Expect = 9.5
 Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 4/40 (10%)
 Frame = +1

Query: 97  WILQVAM--RFHLLDVCYPDYVRVPSICLVETLE--LFVL 204
           WIL ++   R+++LD   PD V++  IC++  L   LFV+
Sbjct: 116 WILMISFLYRYYILDQISPDTVKIVRICILTYLPSLLFVI 155


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,851,205
Number of Sequences: 219361
Number of extensions: 1782220
Number of successful extensions: 5282
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 5131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5279
length of database: 80,573,946
effective HSP length: 105
effective length of database: 57,541,041
effective search space used: 4200495993
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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