ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart28d10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1KR511_HUMAN (Q6L8G4) Keratin-associated protein 5-11 (Keratin-as... 31 0.82
2CQ028_HUMAN (Q8IV36) Protein C17orf28 (Down-regulated in multipl... 30 1.1
3MKTX1_MESMA (P56569) Makatoxin-1 (Makatoxin I) (MkTx I) 29 2.4
4SCM2_MESMA (P58488) Neurotoxin BmK-M2 (BmK M2) (BmKM2) 29 3.1
5SUIS_RABIT (P07768) Sucrase-isomaltase, intestinal [Contains: Su... 29 3.1
6YBBP_ECOLI (P77504) Hypothetical protein ybbP 28 4.1
7MKTX3_MESMA (P59853) Makatoxin-3 precursor (Makatoxin III) (MkTx... 28 4.1
8KRA58_HUMAN (O75690) Keratin-associated protein 5-8 (Keratin-ass... 28 4.1
9SCX4_BUTOM (P59354) Alpha-like neurotoxin IV (Bom IV) (BomIV) (B... 28 5.3
10KR104_HUMAN (P60372) Keratin-associated protein 10-4 (Keratin-as... 28 5.3
11YIY8_SCHPO (Q9UUI5) Hypothetical protein C22F8.08 in chromosome I 28 5.3
12LITAF_HUMAN (Q99732) Lipopolysaccharide-induced tumor necrosis f... 28 5.3
13KRA53_HUMAN (Q6L8H2) Keratin-associated protein 5-3 (Keratin-ass... 28 6.9
14DNAK_THETN (Q8RB68) Chaperone protein dnaK (Heat shock protein 7... 28 6.9
15CTRB_NEIMB (P32014) Capsule polysaccharide export inner-membrane... 28 6.9
16CTRB_NEIMA (P57034) Capsule polysaccharide export inner-membrane... 28 6.9
17NPT1_HUMAN (Q14916) Renal sodium-dependent phosphate transport p... 28 6.9
18LAMA3_MOUSE (Q61789) Laminin alpha-3 chain precursor (Nicein alp... 28 6.9
19DHML_METEX (P00372) Methylamine dehydrogenase light chain precur... 27 9.0
20SUIS_HUMAN (P14410) Sucrase-isomaltase, intestinal [Contains: Su... 27 9.0
21HMDH_ASPTE (Q9Y7D2) 3-hydroxy-3-methylglutaryl-coenzyme A reduct... 27 9.0

>KR511_HUMAN (Q6L8G4) Keratin-associated protein 5-11 (Keratin-associated|
           protein 5.11) (Ultrahigh sulfur keratin-associated
           protein 5.11)
          Length = 156

 Score = 30.8 bits (68), Expect = 0.82
 Identities = 19/53 (35%), Positives = 24/53 (45%)
 Frame = +2

Query: 194 SESCCR*YCPCSGEVKQIHPLSILCESMACKECKKPSSSAWSSNWRPLCWSST 352
           S S C  +C  S   K     S  C+S  CK C   SS   SS ++P C  S+
Sbjct: 93  SSSGCGSFCCQSSCSKPCCCQSSCCQSSCCKPCCCQSSCCQSSCFKPCCCQSS 145



 Score = 27.7 bits (60), Expect = 6.9
 Identities = 20/64 (31%), Positives = 26/64 (40%)
 Frame = +2

Query: 200 SCCR*YCPCSGEVKQIHPLSILCESMACKECKKPSSSAWSSNWRPLCWSSTLPSSLLLRY 379
           +CC+  C  SG        S  C+S   K C   SS   SS  +P C  S+   S   + 
Sbjct: 86  NCCKPCCSSSGCG------SFCCQSSCSKPCCCQSSCCQSSCCKPCCCQSSCCQSSCFK- 138

Query: 380 VGCC 391
             CC
Sbjct: 139 -PCC 141



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>CQ028_HUMAN (Q8IV36) Protein C17orf28 (Down-regulated in multiple cancers-1)|
          Length = 788

 Score = 30.4 bits (67), Expect = 1.1
 Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 5/40 (12%)
 Frame = +2

Query: 272 SMACKECKKPSSSAWSSNWRP-----LCWSSTLPSSLLLR 376
           S A +E ++PS+S+ S  W P     L W S LP   ++R
Sbjct: 660 SQAWREQRRPSTSSASGQWSPTPEWVLSWKSKLPLQTIMR 699



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>MKTX1_MESMA (P56569) Makatoxin-1 (Makatoxin I) (MkTx I)|
          Length = 64

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 12/46 (26%), Positives = 18/46 (39%)
 Frame = +2

Query: 254 LSILCESMACKECKKPSSSAWSSNWRPLCWSSTLPSSLLLRYVGCC 391
           L+  C  +  K   K     W+  +   CW   LP  + +R  G C
Sbjct: 18  LNPYCNDLCTKNGAKSGYCQWAGRYGNACWCIDLPDKVPIRISGSC 63



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>SCM2_MESMA (P58488) Neurotoxin BmK-M2 (BmK M2) (BmKM2)|
          Length = 64

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 12/42 (28%), Positives = 18/42 (42%)
 Frame = +2

Query: 266 CESMACKECKKPSSSAWSSNWRPLCWSSTLPSSLLLRYVGCC 391
           C ++  K   K     WS  +   CW   LP ++ +R  G C
Sbjct: 22  CNNLCTKNGAKSGYCQWSGKYGNGCWCIELPDNVPIRVPGKC 63



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>SUIS_RABIT (P07768) Sucrase-isomaltase, intestinal [Contains: Sucrase (EC|
           3.2.1.48); Isomaltase (EC 3.2.1.10)]
          Length = 1826

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 16/53 (30%), Positives = 25/53 (47%), Gaps = 6/53 (11%)
 Frame = -3

Query: 142 TSERLCDPIASTRVFLPVDRNH------HQSLTWYKLHALIFRTSLIYTNIKH 2
           T+E LC        F P  RNH      HQ   ++   +L+ ++S  Y NI++
Sbjct: 640 TTEELCRRWMQLGAFYPFSRNHNADGFEHQDPAFFGQDSLLVKSSRHYLNIRY 692



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>YBBP_ECOLI (P77504) Hypothetical protein ybbP|
          Length = 804

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = -3

Query: 250 MDLLDLSRTGAVLAATRLTWAFFF 179
           M+L D+ +TGAV   +R+TW + F
Sbjct: 176 MNLADVDKTGAVQPGSRVTWRYKF 199



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>MKTX3_MESMA (P59853) Makatoxin-3 precursor (Makatoxin III) (MkTx III)|
          Length = 85

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 12/46 (26%), Positives = 18/46 (39%)
 Frame = +2

Query: 254 LSILCESMACKECKKPSSSAWSSNWRPLCWSSTLPSSLLLRYVGCC 391
           L+  C  +  K   K     W+  +   CW   LP  + +R  G C
Sbjct: 37  LNPYCNDLCTKNGAKSGYCQWAGRYGNACWCIDLPDKVPIRIPGPC 82



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>KRA58_HUMAN (O75690) Keratin-associated protein 5-8 (Keratin-associated protein|
           5.8) (Ultrahigh sulfur keratin-associated protein 5.8)
           (Keratin, ultra high-sulfur matrix protein B) (UHS
           keratin B) (UHS KerB)
          Length = 187

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 21/64 (32%), Positives = 25/64 (39%), Gaps = 9/64 (14%)
 Frame = +2

Query: 200 SCCR*YC--PCSGEVKQIHPLSI-------LCESMACKECKKPSSSAWSSNWRPLCWSST 352
           SCC+  C  PC  +     P S         C+S  CK C   SS       +P C SS 
Sbjct: 103 SCCQSSCCKPCCSQSSCCKPCSCSSGCGSSCCQSSCCKPCCSQSSCC-----KPCCCSSG 157

Query: 353 LPSS 364
             SS
Sbjct: 158 CGSS 161



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>SCX4_BUTOM (P59354) Alpha-like neurotoxin IV (Bom IV) (BomIV) (Bom4)|
          Length = 65

 Score = 28.1 bits (61), Expect = 5.3
 Identities = 11/42 (26%), Positives = 16/42 (38%)
 Frame = +2

Query: 266 CESMACKECKKPSSSAWSSNWRPLCWSSTLPSSLLLRYVGCC 391
           C  +  K   K     W   +   CW   LP ++ +R  G C
Sbjct: 22  CNDLCTKNGAKSGYCQWLGKYGNACWCEDLPDNVPIRIPGKC 63



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>KR104_HUMAN (P60372) Keratin-associated protein 10-4 (Keratin-associated|
           protein 10.4) (High sulfur keratin-associated protein
           10.4) (Keratin-associated protein 18-4)
           (Keratin-associated protein 18.4)
          Length = 401

 Score = 28.1 bits (61), Expect = 5.3
 Identities = 16/54 (29%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
 Frame = +2

Query: 197 ESCCR*YCPCSGEVKQIHPLSILCESMACKE-CKKPSSS-AWSSNWRPLCWSST 352
           +S C+  C  S   +Q   + + C+++ CK  C + SSS    S+ +P C +S+
Sbjct: 211 QSSCQPACCTSSSCQQACCVPVCCKTVCCKPVCSEDSSSCCQQSSCQPACCTSS 264



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>YIY8_SCHPO (Q9UUI5) Hypothetical protein C22F8.08 in chromosome I|
          Length = 926

 Score = 28.1 bits (61), Expect = 5.3
 Identities = 19/45 (42%), Positives = 27/45 (60%), Gaps = 2/45 (4%)
 Frame = +2

Query: 254 LSILCESMACKECKKPSSSAWSSNWRP--LCWSSTLPSSLLLRYV 382
           L +LC ++  K      SS  SS+ R   LC+ STLP+ LL+RY+
Sbjct: 728 LPLLCLALT-KHTGFRRSSHISSDLRSIALCYLSTLPTPLLMRYI 771



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>LITAF_HUMAN (Q99732) Lipopolysaccharide-induced tumor necrosis factor-alpha|
           factor (LPS-induced TNF-alpha factor) (p53-induced
           protein 7) (Small integral membrane protein of
           lysosome/late endosome)
          Length = 161

 Score = 28.1 bits (61), Expect = 5.3
 Identities = 18/50 (36%), Positives = 20/50 (40%), Gaps = 2/50 (4%)
 Frame = +2

Query: 248 HPLSILCE--SMACKECKKPSSSAWSSNWRPLCWSSTLPSSLLLRYVGCC 391
           HP++ L     M C  C K   S  S N   L W S     LL    GCC
Sbjct: 83  HPITFLDRPIQMCCPSCNKMIVSQLSYNAGALTWLSCGSLCLLGCIAGCC 132



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>KRA53_HUMAN (Q6L8H2) Keratin-associated protein 5-3 (Keratin-associated protein|
           5.3) (Ultrahigh sulfur keratin-associated protein 5.3)
           (Keratin-associated protein 5-9) (Keratin-associated
           protein 5.9) (UHS KerB-like)
          Length = 238

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 21/69 (30%), Positives = 25/69 (36%), Gaps = 7/69 (10%)
 Frame = +2

Query: 179 KKKSPSESCCR*YCPCSGEVKQIHPL-------SILCESMACKECKKPSSSAWSSNWRPL 337
           K      SCC+   PC  +     P        S  C+S  CK C   SS       +P 
Sbjct: 152 KPSCSQSSCCK---PCCSQSSCCKPCCCSSGCGSSCCQSSCCKPCCSQSSCC-----KPC 203

Query: 338 CWSSTLPSS 364
           C SS   SS
Sbjct: 204 CCSSGCGSS 212



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>DNAK_THETN (Q8RB68) Chaperone protein dnaK (Heat shock protein 70) (Heat shock|
           70 kDa protein) (HSP70)
          Length = 606

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 14/45 (31%), Positives = 28/45 (62%), Gaps = 5/45 (11%)
 Frame = +3

Query: 84  LSTGKNTRVDAIGSHNLSE-----VILQQGEFLECEKKKKAQVSL 203
           L+TGK+ ++   GS NLSE     +I +  ++ E ++KK+ ++ +
Sbjct: 462 LATGKSQQITITGSTNLSEEEIQRMINEAKQYEEQDRKKREEIEI 506



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>CTRB_NEIMB (P32014) Capsule polysaccharide export inner-membrane protein ctrB|
          Length = 387

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
 Frame = +2

Query: 251 PLSILCESMACKECKKPSSSAWSSNWRPLCWSST-LPSSLLLRYVG 385
           P+++  E+ A  E KKP   +W     PL W +  +P+ + L Y G
Sbjct: 6   PVAVATETKA--ERKKPKKKSWIKKLSPLFWVTVIIPTVISLVYFG 49



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>CTRB_NEIMA (P57034) Capsule polysaccharide export inner-membrane protein ctrB|
          Length = 387

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
 Frame = +2

Query: 251 PLSILCESMACKECKKPSSSAWSSNWRPLCWSST-LPSSLLLRYVG 385
           P+++  E+ A  E KKP   +W     PL W +  +P+ + L Y G
Sbjct: 6   PVAVATETKA--ERKKPKKKSWIKKLSPLFWVTVIIPTVISLVYFG 49



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>NPT1_HUMAN (Q14916) Renal sodium-dependent phosphate transport protein 1|
           (Sodium/phosphate cotransporter 1) (Na(+)/PI
           cotransporter 1) (Renal sodium-phosphate transport
           protein 1) (Renal Na(+)-dependent phosphate
           cotransporter 1) (Solute carrier family 1
          Length = 465

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 22/93 (23%), Positives = 35/93 (37%), Gaps = 20/93 (21%)
 Frame = -3

Query: 223 GAVLAATRLTWAFFFFSHSKNSPCCRITSER-----LCDPIASTRVFLPVDRNHHQSLTW 59
           GA   A  L W   F+   K+ PC  I+ +      L   ++S+R  LP+         W
Sbjct: 204 GACGCAVCLLWFVLFYDDPKDHPCISISEKEYITSSLVQQVSSSRQSLPIKAILKSLPVW 263

Query: 58  ---------------YKLHALIFRTSLIYTNIK 5
                            L+  +F  S+++ NIK
Sbjct: 264 AISIGSFTFFWSHNIMTLYTPMFINSMLHVNIK 296



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>LAMA3_MOUSE (Q61789) Laminin alpha-3 chain precursor (Nicein alpha subunit)|
          Length = 3333

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 13/41 (31%), Positives = 19/41 (46%)
 Frame = +2

Query: 224 CSGEVKQIHPLSILCESMACKECKKPSSSAWSSNWRPLCWS 346
           C G     HP   L  S+   +CK+  +S   S  +PL W+
Sbjct: 576 CQGSGSVCHPAGTLDSSLGYCQCKQHVASPTCSVCKPLYWN 616



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>DHML_METEX (P00372) Methylamine dehydrogenase light chain precursor (EC|
           1.4.99.3) (MADH)
          Length = 186

 Score = 27.3 bits (59), Expect = 9.0
 Identities = 13/42 (30%), Positives = 19/42 (45%)
 Frame = +2

Query: 266 CESMACKECKKPSSSAWSSNWRPLCWSSTLPSSLLLRYVGCC 391
           C   +   C  P +   SS+W   C++ T   S L+ Y  CC
Sbjct: 93  CSGGSLTSCP-PGTKLASSSWVASCYNPTDKQSYLISYRDCC 133



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>SUIS_HUMAN (P14410) Sucrase-isomaltase, intestinal [Contains: Sucrase (EC|
           3.2.1.48); Isomaltase (EC 3.2.1.10)]
          Length = 1826

 Score = 27.3 bits (59), Expect = 9.0
 Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 6/53 (11%)
 Frame = -3

Query: 142 TSERLCDPIASTRVFLPVDRNH------HQSLTWYKLHALIFRTSLIYTNIKH 2
           T+E LC        F P  RNH      HQ   ++  ++L+ ++S  Y  I++
Sbjct: 640 TTEELCRRWMQLGAFYPFSRNHNSDGYEHQDPAFFGQNSLLVKSSRQYLTIRY 692



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>HMDH_ASPTE (Q9Y7D2) 3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC|
           1.1.1.34) (HMG-CoA reductase)
          Length = 1068

 Score = 27.3 bits (59), Expect = 9.0
 Identities = 14/49 (28%), Positives = 24/49 (48%)
 Frame = -1

Query: 303 DGFLHSLHAIDSQRMDSGWICLTSPEQGQY*RQQDSLGLFFFSRIPKIH 157
           DGFL ++  I S++ D  W   +  E+G        LG  + S + ++H
Sbjct: 142 DGFLQAVEIIPSEKEDDSWRLRSPREEGSPRSLGHWLGSSWLSFLHRVH 190


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,109,667
Number of Sequences: 219361
Number of extensions: 993692
Number of successful extensions: 2588
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 2515
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2588
length of database: 80,573,946
effective HSP length: 106
effective length of database: 57,321,680
effective search space used: 1375720320
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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