ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart28b04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SRA24_CAEEL (O62368) Serpentine receptor class alpha-24 (Protein... 32 1.4
2RNP1_SCHPO (Q9P7W9) Probable ribonuclease P protein subunit 1 (E... 32 1.4
3ACM2_CAEEL (Q09388) Probable muscarinic acetylcholine receptor g... 31 1.8
4SYFB_CAMJR (Q5HUR2) Phenylalanyl-tRNA synthetase beta chain (EC ... 31 1.8
5SYFB_CAMJE (Q9PP35) Phenylalanyl-tRNA synthetase beta chain (EC ... 31 1.8
6RPA2_PONPY (Q5REE8) DNA-directed RNA polymerase I 135 kDa polype... 31 2.3
7RPA2_HUMAN (Q9H9Y6) DNA-directed RNA polymerase I 135 kDa polype... 30 5.2
8PKND_CHLCV (Q822K5) Serine/threonine-protein kinase pknD (EC 2.7... 29 8.9

>SRA24_CAEEL (O62368) Serpentine receptor class alpha-24 (Protein sra-24)|
          Length = 339

 Score = 31.6 bits (70), Expect = 1.4
 Identities = 34/128 (26%), Positives = 52/128 (40%)
 Frame = -1

Query: 405 VPMITAKIYVVQMRCNVLWLNRGFACLSLYVVIFSPLLKCLEEFSKTLGEGMIQFYPSVL 226
           V +I    Y  ++    LW N  F+  S  +++   LL  +   +  L   + Q Y S++
Sbjct: 36  VTVILLSYYFARLAIRTLWKNNIFSN-STRLILLVCLLNSIIHQTTMLEIRIRQIYRSIV 94

Query: 225 ESSNNFGLK*HRTNLQCSLTSYYVLPPFLNMSLFEDFIRGLHSKQNERMYTLKYVCSLQV 46
            +S    L  H T  +  L  YY+   F   S+F      L S      YT KY  S Q 
Sbjct: 95  FASEPCRLPFHFTECEVELFVYYLTTYFSTYSVFSLAFDRLIS-----CYTPKYYLSHQY 149

Query: 45  KLLKRLIF 22
            +   L+F
Sbjct: 150 YVSIFLLF 157



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>RNP1_SCHPO (Q9P7W9) Probable ribonuclease P protein subunit 1 (EC 3.1.26.5)|
          Length = 217

 Score = 31.6 bits (70), Expect = 1.4
 Identities = 20/55 (36%), Positives = 29/55 (52%)
 Frame = +2

Query: 305 KITTYSERQAKPRFNHSTLHLIWTTYILAVIIGTVG*AHRSQRMVSEHQGQSKEV 469
           KI  YS+      F H  LH +W +YIL VI G  G +  ++   +E+QG   +V
Sbjct: 89  KIADYSQ------FKH--LHSMWCSYILEVIAGCTGESLMAKLAKAEYQGAYMQV 135



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>ACM2_CAEEL (Q09388) Probable muscarinic acetylcholine receptor gar-2|
           (G-protein linked acetylcholine receptor 2)
          Length = 627

 Score = 31.2 bits (69), Expect = 1.8
 Identities = 32/128 (25%), Positives = 54/128 (42%), Gaps = 11/128 (8%)
 Frame = +2

Query: 14  PFLNISLFKSFTWR-----LHTYFRVYIRSFCFECSPLMKSSKRLIFRNGGST**LVSEH 178
           P+LN+ ++ S+ W      L+ Y+ +Y            +++K+L  ++   T     + 
Sbjct: 168 PYLNMGMYISYYWTTLFVMLYLYWGIY------------RAAKKLALKSDQKT-----KR 210

Query: 179 CKFVRCYLRPKLLLDSRTLG*NWIIPSPNVLENSSRHFSSGEKITTYSERQAK-----PR 343
              +    RP++ + +   G N    SPN   NSS+ F +    TT    Q       P 
Sbjct: 211 LALLTEMRRPEVSVRTSDAG-NSSSDSPNDTSNSSKCFRTAPPTTTVQTTQTNVGTPPPV 269

Query: 344 F-NHSTLH 364
           F NH TLH
Sbjct: 270 FRNHMTLH 277



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>SYFB_CAMJR (Q5HUR2) Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)|
           (Phenylalanine--tRNA ligase beta chain) (PheRS)
          Length = 773

 Score = 31.2 bits (69), Expect = 1.8
 Identities = 21/79 (26%), Positives = 37/79 (46%), Gaps = 6/79 (7%)
 Frame = -1

Query: 249 IQFYPSVLESSNNFGLK*HRTNLQCSLTSYYVLPPFLNMSLFED-----FIRGLHSK-QN 88
           +QFY  +L+  N  G      + +   + Y +L P+    ++       FI  LH K +N
Sbjct: 601 VQFYDFLLDIKNIIG------DFKLKSSKYNILSPYEQADIYLSDIKVGFIGRLHLKIEN 654

Query: 87  ERMYTLKYVCSLQVKLLKR 31
           ER     Y+C L + L+++
Sbjct: 655 ERDLPKTYICELDLDLIRQ 673



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>SYFB_CAMJE (Q9PP35) Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)|
           (Phenylalanine--tRNA ligase beta chain) (PheRS)
          Length = 773

 Score = 31.2 bits (69), Expect = 1.8
 Identities = 21/79 (26%), Positives = 37/79 (46%), Gaps = 6/79 (7%)
 Frame = -1

Query: 249 IQFYPSVLESSNNFGLK*HRTNLQCSLTSYYVLPPFLNMSLFED-----FIRGLHSK-QN 88
           +QFY  +L+  N  G      + +   + Y +L P+    ++       FI  LH K +N
Sbjct: 601 VQFYDFLLDIKNIIG------DFKLKSSKYNILSPYEQADIYLSDIKVGFIGRLHLKIEN 654

Query: 87  ERMYTLKYVCSLQVKLLKR 31
           ER     Y+C L + L+++
Sbjct: 655 ERDLPKTYICELDLDLIRQ 673



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>RPA2_PONPY (Q5REE8) DNA-directed RNA polymerase I 135 kDa polypeptide (EC|
            2.7.7.6) (RNA polymerase I subunit 2) (RPA135)
          Length = 1136

 Score = 30.8 bits (68), Expect = 2.3
 Identities = 30/96 (31%), Positives = 47/96 (48%)
 Frame = -1

Query: 342  RGFACLSLYVVIFSPLLKCLEEFSKTLGEGMIQFYPSVLESSNNFGLK*HRTNLQCSLTS 163
            RGFA  S+Y   F  L + +++   +L  GM    P +L+  ++ GL      LQ     
Sbjct: 766  RGFAHGSVYKSEFIDLSEKIKQGDSSLVFGMKPGDPRILQKLDDDGLPFIGAKLQYG-DP 824

Query: 162  YYVLPPFLNMSLFEDFIRGLHSKQNERMYTLKYVCS 55
            YY    +LN++  E F+    SK+N  +  +K VCS
Sbjct: 825  YY---SYLNLNTGESFVMYYKSKENCVVDNIK-VCS 856



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>RPA2_HUMAN (Q9H9Y6) DNA-directed RNA polymerase I 135 kDa polypeptide (EC|
            2.7.7.6) (RNA polymerase I subunit 2) (RPA135)
          Length = 1135

 Score = 29.6 bits (65), Expect = 5.2
 Identities = 30/96 (31%), Positives = 47/96 (48%)
 Frame = -1

Query: 342  RGFACLSLYVVIFSPLLKCLEEFSKTLGEGMIQFYPSVLESSNNFGLK*HRTNLQCSLTS 163
            RGFA  S+Y   F  L + +++   +L  G+    P VL+  ++ GL      LQ     
Sbjct: 766  RGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYG-DP 824

Query: 162  YYVLPPFLNMSLFEDFIRGLHSKQNERMYTLKYVCS 55
            YY    +LN++  E F+    SK+N  +  +K VCS
Sbjct: 825  YY---SYLNLNTGESFVMYYKSKENCVVDNIK-VCS 856



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>PKND_CHLCV (Q822K5) Serine/threonine-protein kinase pknD (EC 2.7.11.1)|
          Length = 930

 Score = 28.9 bits (63), Expect = 8.9
 Identities = 15/50 (30%), Positives = 26/50 (52%)
 Frame = -1

Query: 195 HRTNLQCSLTSYYVLPPFLNMSLFEDFIRGLHSKQNERMYTLKYVCSLQV 46
           HR +L   L + +V P  +N S  E F++ LH K  + ++     C+L +
Sbjct: 607 HRISLVFMLLALHVAPESINASEEEHFLQNLHGKIQDTLF-----CNLDI 651


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 71,121,460
Number of Sequences: 219361
Number of extensions: 1369904
Number of successful extensions: 2935
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 2907
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2935
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3927707336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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