ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart27c03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RETO_ESCCA (P30986) Reticuline oxidase precursor (EC 1.21.3.3) (... 83 5e-16
2RETO_PAPSO (P93479) Reticuline oxidase precursor (EC 1.21.3.3) (... 76 5e-14
3MCRA_STRLA (P43485) Mitomycin radical oxidase (EC 1.5.3.-) 32 1.4
4ELOA2_HUMAN (Q8IYF1) RNA polymerase II transcription factor SIII... 30 4.0
5RNH2_PSE14 (Q48F73) Ribonuclease HII (EC 3.1.26.4) (RNase HII) 29 8.9

>RETO_ESCCA (P30986) Reticuline oxidase precursor (EC 1.21.3.3)|
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase)
          Length = 538

 Score = 82.8 bits (203), Expect = 5e-16
 Identities = 43/108 (39%), Positives = 60/108 (55%), Gaps = 6/108 (5%)
 Frame = -1

Query: 506 IQYMNFWSMASGDGAVQ-TRWIREFYAFMAPFVSSSPREAYFNYRDLDLG-----ENVVV 345
           ++Y+  W+ +      +   W+ + Y FM PFVS +PR  Y N+ DLDLG        VV
Sbjct: 416 VEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDWGNKTVV 475

Query: 344 GNVSSFQAGMVWGQKYFKGNYQRLAMAKAQIDPDDYFRNEQSIPPFAN 201
            N  + +    WG+ YF  NY+RL  AK  IDP++ F + QSIPP AN
Sbjct: 476 NN--AIEISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPMAN 521



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>RETO_PAPSO (P93479) Reticuline oxidase precursor (EC 1.21.3.3)|
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase)
          Length = 535

 Score = 76.3 bits (186), Expect = 5e-14
 Identities = 37/86 (43%), Positives = 52/86 (60%), Gaps = 4/86 (4%)
 Frame = -1

Query: 455 TRWIREFYAFMAPFVSSSPREAYFNYRDLDLGENVVVGNVSS----FQAGMVWGQKYFKG 288
           + W+ +FY ++ PFVS  PR  Y N+ DLD+G  +   N SS     +    WG++YF  
Sbjct: 438 SEWLAKFYDYLEPFVSKEPRVGYVNHIDLDIG-GIDWRNKSSTTNAVEIARNWGERYFSS 496

Query: 287 NYQRLAMAKAQIDPDDYFRNEQSIPP 210
           NY+RL  AK  IDP++ F + QSIPP
Sbjct: 497 NYERLVKAKTLIDPNNVFNHPQSIPP 522



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>MCRA_STRLA (P43485) Mitomycin radical oxidase (EC 1.5.3.-)|
          Length = 447

 Score = 31.6 bits (70), Expect = 1.4
 Identities = 14/30 (46%), Positives = 18/30 (60%)
 Frame = -1

Query: 299 YFKGNYQRLAMAKAQIDPDDYFRNEQSIPP 210
           Y   ++ RL   KAQ DPD+ FR   +IPP
Sbjct: 413 YTPADFARLRAVKAQYDPDNMFRVNFNIPP 442



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>ELOA2_HUMAN (Q8IYF1) RNA polymerase II transcription factor SIII subunit A2|
           (Elongin A2) (EloA2) (Transcription elongation factor B
           polypeptide 3B)
          Length = 753

 Score = 30.0 bits (66), Expect = 4.0
 Identities = 13/32 (40%), Positives = 15/32 (46%)
 Frame = +3

Query: 396 PRAGAHERRHERVELPDPPGLHGPVPRRHRPE 491
           PR+ +H   H R     PPG   P PR H  E
Sbjct: 119 PRSPSHSPEHRRTARRTPPGQQRPHPRSHSRE 150



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>RNH2_PSE14 (Q48F73) Ribonuclease HII (EC 3.1.26.4) (RNase HII)|
          Length = 222

 Score = 28.9 bits (63), Expect = 8.9
 Identities = 19/46 (41%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
 Frame = +3

Query: 186 VRSPAIGKWRDALLVPEVVVGVD-LGLGHRQPLVVTLEVLLAPDHP 320
           +RSP +    D  LV ++V GVD +G G     VVT  V+L P  P
Sbjct: 1   MRSPIMQTGLDFTLVEDLVAGVDEVGRGPLCGAVVTAAVILDPARP 46


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,177,667
Number of Sequences: 219361
Number of extensions: 1148439
Number of successful extensions: 3040
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2939
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3038
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3927707336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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