ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart25f10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1gb|M36650.1|BLYGAPDH Barley glyceraldehyde-3-phosphate dehydroge... 331 6e-88
2ref|XM_506852.1| PREDICTED Oryza sativa (japonica cultivar-group... 42 0.80
3ref|XM_466582.1| Oryza sativa (japonica cultivar-group), mRNA 42 0.80
4ref|XM_614616.2| PREDICTED: Bos taurus similar to solute carrier... 42 0.80
5ref|XM_864288.1| PREDICTED: Bos taurus similar to solute carrier... 42 0.80
6gb|DQ083193.1| Oryza sativa (indica cultivar-group) clone 8S4Eb0... 42 0.80
7dbj|AP008208.1| Oryza sativa (japonica cultivar-group) genomic D... 42 0.80
8dbj|AP005385.3| Oryza sativa (japonica cultivar-group) genomic D... 42 0.80
9dbj|AK099086.1| Oryza sativa (japonica cultivar-group) cDNA clon... 42 0.80
10dbj|AK062215.1| Oryza sativa (japonica cultivar-group) cDNA clon... 42 0.80
11gb|AC158155.4| Mus musculus chromosome 3, clone RP23-167L9, comp... 40 3.2
12gb|AC008284.7| Drosophila melanogaster clone BACR03M22, complete... 40 3.2
13gb|AC027133.3| Oryza sativa (japonica cultivar-group) chromosome... 40 3.2
14gb|AC136843.6| Oryza sativa (japonica cultivar-group) chromosome... 40 3.2
15gb|AC099020.2| Drosophila melanogaster clone BACR30H01, complete... 40 3.2
16emb|CR450741.5| Zebrafish DNA sequence from clone CH211-269F16 i... 40 3.2
17emb|AL606691.4|OSJN00081 Oryza sativa genomic DNA, chromosome 4,... 40 3.2
18gb|AC106753.3| Homo sapiens chromosome 5 clone RP11-174H5, compl... 40 3.2
19gb|AC099017.1| Drosophila melanogaster, chromosome 2L, region 37... 40 3.2
20gb|AC006084.1|AC006084 Homo sapiens chromosome 5, Pac clone 9c13... 40 3.2
21dbj|AP008210.1| Oryza sativa (japonica cultivar-group) genomic D... 40 3.2
22dbj|AP008218.1| Oryza sativa (japonica cultivar-group) genomic D... 40 3.2
23dbj|AP008217.1| Oryza sativa (japonica cultivar-group) genomic D... 40 3.2
24gb|AC156831.2| Mus musculus BAC clone RP24-276N6 from chromosome... 40 3.2
25gb|AC096670.1| Homo sapiens BAC clone RP11-438K19 from 2, comple... 40 3.2
26emb|BX537129.6| Zebrafish DNA sequence from clone CH211-236I20 i... 40 3.2
27dbj|BA000004.3| Bacillus halodurans C-125 DNA, complete genome 40 3.2
28dbj|AP005694.3| Oryza sativa (japonica cultivar-group) genomic D... 40 3.2
29dbj|AP005316.3| Oryza sativa (japonica cultivar-group) genomic D... 40 3.2
30emb|AL442105.2| Oryza sativa genomic DNA, chromosome 4, BAC clon... 40 3.2
31gb|AE003751.3| Drosophila melanogaster chromosome 3R, section 89... 40 3.2
32gb|AE003663.4| Drosophila melanogaster chromosome 2L, section 72... 40 3.2
33gb|AC005128.1|AC005128 Drosophila melanogaster, chromosome 2L, r... 40 3.2
34emb|BX284664.8| Zebrafish DNA sequence from clone DKEY-7I23 in l... 40 3.2
35gb|DP000010.1| Oryza sativa (japonica cultivar-group) chromosome... 40 3.2
36gb|DP000011.1| Oryza sativa (japonica cultivar-group) chromosome... 40 3.2
37emb|AL591973.1| Listeria monocytogenes strain EGD, complete geno... 40 3.2
38emb|AL831803.4|CNS08CAG Oryza sativa chromosome 12, . BAC OSJNBb... 40 3.2
39gb|AE017262.2| Listeria monocytogenes str. 4b F2365, complete ge... 40 3.2

>gb|M36650.1|BLYGAPDH Barley glyceraldehyde-3-phosphate dehydrogenase mRNA, 3' end
          Length = 1141

 Score =  331 bits (167), Expect = 6e-88
 Identities = 222/240 (92%), Gaps = 2/240 (0%)
 Strand = Plus / Minus

                                                                        
Query: 6    aacccctgacgatgagtgaaaataactcatgatttagttcaagcaagtacataataccac 65
            ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1141 aacccatgacgatgagtgaaaataactcatgatttagttcaagcaagtacataataccac 1082

                                                                        
Query: 66   caaaatgggagccgctaagctcaaccaaccatcaggatacatccaataaaagggnnnnnn 125
            ||||||| |||||||||||||||||||||||||||||| ||||||||||||||       
Sbjct: 1081 caaaatgtgagccgctaagctcaaccaaccatcaggat-catccaataaaaggttttttt 1023

                                                                        
Query: 126  nagttgccataattccatacaaaaatttattctcggaagagggtgtacactcttcccttc 185
             ||||||||||||||||||||||||||||||||||||||||| |||||||||||| ||||
Sbjct: 1022 tagttgccataattccatacaaaaatttattctcggaagaggttgtacactcttctcttc 963

                                                                        
Query: 186  tctgggggaaaccacactcccctggatcttcatggtttggctcatttacttggggctgtg 245
            ||||||| |||||||||| ||||| ||||||||| |||||||||||||||||| ||||||
Sbjct: 962  tctggggcaaaccacact-ccctgtatcttcatgctttggctcatttacttggtgctgtg 904



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>ref|XM_506852.1| PREDICTED Oryza sativa (japonica cultivar-group), OJ1791_B03.34 mRNA
          Length = 1460

 Score = 42.1 bits (21), Expect = 0.80
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                 
Query: 147  aaaatttattctcggaagagg 167
            |||||||||||||||||||||
Sbjct: 1323 aaaatttattctcggaagagg 1303



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>ref|XM_466582.1| Oryza sativa (japonica cultivar-group),  mRNA
          Length = 1456

 Score = 42.1 bits (21), Expect = 0.80
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                 
Query: 147  aaaatttattctcggaagagg 167
            |||||||||||||||||||||
Sbjct: 1339 aaaatttattctcggaagagg 1319



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>ref|XM_614616.2| PREDICTED: Bos taurus similar to solute carrier family 25 member 24
            isoform 2, transcript variant 2 (LOC534742), mRNA
          Length = 3261

 Score = 42.1 bits (21), Expect = 0.80
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                 
Query: 37   atttagttcaagcaagtacat 57
            |||||||||||||||||||||
Sbjct: 1695 atttagttcaagcaagtacat 1675



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>ref|XM_864288.1| PREDICTED: Bos taurus similar to solute carrier family 25 member 24
            isoform 2, transcript variant 3 (LOC534742), mRNA
          Length = 3324

 Score = 42.1 bits (21), Expect = 0.80
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                 
Query: 37   atttagttcaagcaagtacat 57
            |||||||||||||||||||||
Sbjct: 1758 atttagttcaagcaagtacat 1738



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>gb|DQ083193.1| Oryza sativa (indica cultivar-group) clone 8S4Eb01 mRNA sequence
          Length = 206

 Score = 42.1 bits (21), Expect = 0.80
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                
Query: 147 aaaatttattctcggaagagg 167
           |||||||||||||||||||||
Sbjct: 38  aaaatttattctcggaagagg 18



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>dbj|AP008208.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 2, complete
                sequence
          Length = 35954743

 Score = 42.1 bits (21), Expect = 0.80
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                     
Query: 147      aaaatttattctcggaagagg 167
                |||||||||||||||||||||
Sbjct: 23549735 aaaatttattctcggaagagg 23549715



 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Minus

                                        
Query: 207      ctggatcttcatggtttggctcat 230
                ||||||||||||||||||| ||||
Sbjct: 16474517 ctggatcttcatggtttggatcat 16474494



 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Minus

                                        
Query: 207      ctggatcttcatggtttggctcat 230
                ||||||||||||||||||| ||||
Sbjct: 10568791 ctggatcttcatggtttggatcat 10568768



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>dbj|AP005385.3| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 2, BAC
              clone:OJ1791_B03
          Length = 166214

 Score = 42.1 bits (21), Expect = 0.80
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                   
Query: 147    aaaatttattctcggaagagg 167
              |||||||||||||||||||||
Sbjct: 115418 aaaatttattctcggaagagg 115398



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>dbj|AK099086.1| Oryza sativa (japonica cultivar-group) cDNA clone:J023009G10, full
            insert sequence
          Length = 1460

 Score = 42.1 bits (21), Expect = 0.80
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                 
Query: 147  aaaatttattctcggaagagg 167
            |||||||||||||||||||||
Sbjct: 1323 aaaatttattctcggaagagg 1303



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>dbj|AK062215.1| Oryza sativa (japonica cultivar-group) cDNA clone:001-047-A09, full
            insert sequence
          Length = 1455

 Score = 42.1 bits (21), Expect = 0.80
 Identities = 21/21 (100%)
 Strand = Plus / Minus

                                 
Query: 147  aaaatttattctcggaagagg 167
            |||||||||||||||||||||
Sbjct: 1338 aaaatttattctcggaagagg 1318



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>gb|AC158155.4| Mus musculus chromosome 3, clone RP23-167L9, complete sequence
          Length = 205345

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 174    actcttcccttctctggggg 193
              ||||||||||||||||||||
Sbjct: 194495 actcttcccttctctggggg 194476



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>gb|AC008284.7| Drosophila melanogaster clone BACR03M22, complete sequence
          Length = 161996

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 134    ataattccatacaaaaattt 153
              ||||||||||||||||||||
Sbjct: 114326 ataattccatacaaaaattt 114307



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>gb|AC027133.3| Oryza sativa (japonica cultivar-group) chromosome 12 clone
             OSJNBb0003F09, complete sequence
          Length = 159600

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                     
Query: 207   ctggatcttcatggtttggctcat 230
             ||||||||||||||||||| ||||
Sbjct: 26643 ctggatcttcatggtttgggtcat 26666



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>gb|AC136843.6| Oryza sativa (japonica cultivar-group) chromosome 11 clone
             OSJNBa0094M06, complete sequence
          Length = 135583

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Minus

                                     
Query: 207   ctggatcttcatggtttggctcat 230
             ||||||||||||||||||| ||||
Sbjct: 62593 ctggatcttcatggtttggatcat 62570



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>gb|AC099020.2| Drosophila melanogaster clone BACR30H01, complete sequence
          Length = 155557

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 16     gatgagtgaaaataactcat 35
              ||||||||||||||||||||
Sbjct: 143705 gatgagtgaaaataactcat 143686



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>emb|CR450741.5| Zebrafish DNA sequence from clone CH211-269F16 in linkage group 24,
              complete sequence
          Length = 157603

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 221    tttggctcatttacttgggg 240
              ||||||||||||||||||||
Sbjct: 134298 tttggctcatttacttgggg 134279



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>emb|AL606691.4|OSJN00081 Oryza sativa genomic DNA, chromosome 4, BAC clone: OSJNBa0060N03,
              complete sequence
          Length = 179798

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 133    cataattccatacaaaaatt 152
              ||||||||||||||||||||
Sbjct: 151831 cataattccatacaaaaatt 151850



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>gb|AC106753.3| Homo sapiens chromosome 5 clone RP11-174H5, complete sequence
          Length = 95620

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 216   catggtttggctcatttact 235
             ||||||||||||||||||||
Sbjct: 21298 catggtttggctcatttact 21279



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>gb|AC099017.1| Drosophila melanogaster, chromosome 2L, region 37D-37E, BAC clone
              BACR27M12, complete sequence
          Length = 157766

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 16     gatgagtgaaaataactcat 35
              ||||||||||||||||||||
Sbjct: 127254 gatgagtgaaaataactcat 127273



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>gb|AC006084.1|AC006084 Homo sapiens chromosome 5, Pac clone 9c13 (LBNL H127), complete
             sequence
          Length = 121739

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 216   catggtttggctcatttact 235
             ||||||||||||||||||||
Sbjct: 74758 catggtttggctcatttact 74739



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>dbj|AP008210.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 4, complete
                sequence
          Length = 35498469

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                    
Query: 133      cataattccatacaaaaatt 152
                ||||||||||||||||||||
Sbjct: 30884310 cataattccatacaaaaatt 30884329



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>dbj|AP008218.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 12, complete
                sequence
          Length = 27566993

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                        
Query: 207      ctggatcttcatggtttggctcat 230
                ||||||||||||||||||| ||||
Sbjct: 26901676 ctggatcttcatggtttgggtcat 26901699



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>dbj|AP008217.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 11, complete
                sequence
          Length = 28386948

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Minus

                                        
Query: 207      ctggatcttcatggtttggctcat 230
                ||||||||||||||||||| ||||
Sbjct: 15889995 ctggatcttcatggtttggatcat 15889972



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>gb|AC156831.2| Mus musculus BAC clone RP24-276N6 from chromosome 9, complete sequence
          Length = 152361

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 180   cccttctctgggggaaacca 199
             ||||||||||||||||||||
Sbjct: 69230 cccttctctgggggaaacca 69211



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>gb|AC096670.1| Homo sapiens BAC clone RP11-438K19 from 2, complete sequence
          Length = 181497

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 175   ctcttcccttctctggggga 194
             ||||||||||||||||||||
Sbjct: 96278 ctcttcccttctctggggga 96259



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>emb|BX537129.6| Zebrafish DNA sequence from clone CH211-236I20 in linkage group 18,
             complete sequence
          Length = 166655

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 221   tttggctcatttacttgggg 240
             ||||||||||||||||||||
Sbjct: 11156 tttggctcatttacttgggg 11175



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>dbj|BA000004.3| Bacillus halodurans C-125 DNA, complete genome
          Length = 4202352

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                       
Query: 32      tcatgatttagttcaagcaagtac 55
               |||||||||||| |||||||||||
Sbjct: 2817665 tcatgatttagtacaagcaagtac 2817688



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>dbj|AP005694.3| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 2, BAC
              clone:OSJNBa0063E14
          Length = 174415

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Minus

                                      
Query: 207    ctggatcttcatggtttggctcat 230
              ||||||||||||||||||| ||||
Sbjct: 149959 ctggatcttcatggtttggatcat 149936



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>dbj|AP005316.3| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 2, PAC
             clone:P0527E02
          Length = 176826

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Minus

                                     
Query: 207   ctggatcttcatggtttggctcat 230
             ||||||||||||||||||| ||||
Sbjct: 47932 ctggatcttcatggtttggatcat 47909



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>emb|AL442105.2| Oryza sativa genomic DNA, chromosome 4, BAC clone: H0702G05, complete
             sequence
          Length = 134586

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 133   cataattccatacaaaaatt 152
             ||||||||||||||||||||
Sbjct: 60884 cataattccatacaaaaatt 60903



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>gb|AE003751.3| Drosophila melanogaster chromosome 3R, section 89 of 118 of the complete
              sequence
          Length = 307443

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 134    ataattccatacaaaaattt 153
              ||||||||||||||||||||
Sbjct: 248319 ataattccatacaaaaattt 248338



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>gb|AE003663.4| Drosophila melanogaster chromosome 2L, section 72 of 83 of the complete
             sequence
          Length = 290667

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 16    gatgagtgaaaataactcat 35
             ||||||||||||||||||||
Sbjct: 98873 gatgagtgaaaataactcat 98892



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>gb|AC005128.1|AC005128 Drosophila melanogaster, chromosome 2L, region 37F1-37F6, P1 clone
             DS08491, complete sequence
          Length = 60040

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 16    gatgagtgaaaataactcat 35
             ||||||||||||||||||||
Sbjct: 49018 gatgagtgaaaataactcat 48999



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>emb|BX284664.8| Zebrafish DNA sequence from clone DKEY-7I23 in linkage group 8,
             complete sequence
          Length = 228202

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 136   aattccatacaaaaatttat 155
             ||||||||||||||||||||
Sbjct: 28677 aattccatacaaaaatttat 28658



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>gb|DP000010.1| Oryza sativa (japonica cultivar-group) chromosome 11, complete sequence
          Length = 28369397

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Minus

                                        
Query: 207      ctggatcttcatggtttggctcat 230
                ||||||||||||||||||| ||||
Sbjct: 15977371 ctggatcttcatggtttggatcat 15977348



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>gb|DP000011.1| Oryza sativa (japonica cultivar-group) chromosome 12, complete sequence
          Length = 27492551

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                        
Query: 207      ctggatcttcatggtttggctcat 230
                ||||||||||||||||||| ||||
Sbjct: 26829846 ctggatcttcatggtttgggtcat 26829869



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>emb|AL591973.1| Listeria monocytogenes strain EGD, complete genome, segment 1/12
          Length = 200050

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 45    caagcaagtacataatacca 64
             ||||||||||||||||||||
Sbjct: 17524 caagcaagtacataatacca 17505



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>emb|AL831803.4|CNS08CAG Oryza sativa chromosome 12, . BAC OSJNBb0016P08 of library OSJNBb from
              chromosome 12 of cultivar Nipponbare of ssp. japonica of
              Oryza sativa (rice), complete sequence
          Length = 142370

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                      
Query: 207    ctggatcttcatggtttggctcat 230
              ||||||||||||||||||| ||||
Sbjct: 105643 ctggatcttcatggtttgggtcat 105666



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>gb|AE017262.2| Listeria monocytogenes str. 4b F2365, complete genome
          Length = 2905187

 Score = 40.1 bits (20), Expect = 3.2
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 45    caagcaagtacataatacca 64
             ||||||||||||||||||||
Sbjct: 19606 caagcaagtacataatacca 19587


  Database: nt
    Posted date:  May 29, 2006 11:10 AM
  Number of letters in database: 3,984,495,279
  Number of sequences in database:  917,343
  
  Database: /shigen/export/home/twatanab/db/nt/nt.01
    Posted date:  May 29, 2006 11:16 AM
  Number of letters in database: 3,988,174,986
  Number of sequences in database:  835,257
  
  Database: /shigen/export/home/twatanab/db/nt/nt.02
    Posted date:  May 29, 2006 11:21 AM
  Number of letters in database: 3,991,246,324
  Number of sequences in database:  771,481
  
  Database: /shigen/export/home/twatanab/db/nt/nt.03
    Posted date:  May 29, 2006 11:27 AM
  Number of letters in database: 3,990,718,311
  Number of sequences in database:  977,174
  
  Database: /shigen/export/home/twatanab/db/nt/nt.04
    Posted date:  May 29, 2006 11:29 AM
  Number of letters in database: 1,278,410,368
  Number of sequences in database:  400,813
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 2,799,627
Number of Sequences: 3902068
Number of extensions: 2799627
Number of successful extensions: 44786
Number of sequences better than 10.0: 39
Number of HSP's better than 10.0 without gapping: 41
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 44662
Number of HSP's gapped (non-prelim): 122
length of query: 245
length of database: 17,233,045,268
effective HSP length: 22
effective length of query: 223
effective length of database: 17,147,199,772
effective search space: 3823825549156
effective search space used: 3823825549156
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)
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