ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart25f08
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1POLG_EBHSG (Q96725) Genome polyprotein [Contains: p16; p23; Heli... 33 0.22
2CLF1_DEBHA (Q6BSP7) Pre-mRNA-splicing factor CLF1 31 0.65
3BCHD_HELMO (Q9ZGE6) Magnesium-chelatase 67 kDa subunit (EC 6.6.1... 30 1.9
4PK3CD_MOUSE (O35904) Phosphatidylinositol-4,5-bisphosphate 3-kin... 29 2.5
5MNT3_CANAL (P87207) Probable mannosyltransferase MNT3 (EC 2.4.1.... 28 5.5
6RIM13_YEAST (Q03792) Calpain-like protease 1 (EC 3.4.22.-) (Cyst... 28 5.5
7HMDH_SCHMA (P16237) 3-hydroxy-3-methylglutaryl-coenzyme A reduct... 28 7.2
8RDS_BOVIN (P17810) Peripherin (Retinal degeneration slow protein) 27 9.4
9SYT_BACTN (Q8AAP2) Threonyl-tRNA synthetase (EC 6.1.1.3) (Threon... 27 9.4
10LEPA_TREDE (P60794) GTP-binding protein lepA 27 9.4

>POLG_EBHSG (Q96725) Genome polyprotein [Contains: p16; p23; Helicase (2C-like|
           protein) (P2C); 3A-like protein; Viral genome-linked
           protein (VPg); Thiol protease P3C (EC 3.4.22.-);
           RNA-directed RNA polymerase (EC 2.7.7.48); Capsid
           protein VP60, subgenomic
          Length = 2334

 Score = 32.7 bits (73), Expect = 0.22
 Identities = 32/102 (31%), Positives = 41/102 (40%), Gaps = 21/102 (20%)
 Frame = +2

Query: 2   RCHMFFISITIHD-RNDQMEGSQGLQRHGQRKW-----------------HNL**P*FKF 127
           R   F+  + I D RN  +EG Q   RHG +                   HN+  P F F
Sbjct: 619 RATAFYRRVIIVDVRNKAVEGWQST-RHGSKPGKHCYTKDMSHLTFQVYPHNMPAPGFVF 677

Query: 128 ANCSKLKYQHAPNITKYNRNLDKISNQKQLSEY---TLHRFV 244
                +K Q AP   KYN  LD I N+   + +   T H FV
Sbjct: 678 VGEKLVKSQVAPRELKYNELLDMIKNEHPDANFEGATKHEFV 719



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>CLF1_DEBHA (Q6BSP7) Pre-mRNA-splicing factor CLF1|
          Length = 714

 Score = 31.2 bits (69), Expect = 0.65
 Identities = 24/83 (28%), Positives = 39/83 (46%), Gaps = 4/83 (4%)
 Frame = +2

Query: 125 FANCSKLKYQHAPNITKYNR---NLDKISNQKQLSEYTLHRFVDYLPRSSSIWFKLCRT* 295
           F    ++  QH P  T Y +   +   I++ + L    L R V  LPR   +WF   +T 
Sbjct: 92  FERALEVNVQHIPFWTHYIQFELSHKNITHARNL----LDRAVTTLPRVDKLWFLYVQT- 146

Query: 296 FHSTVKDTEAATVLF-RWIP*NP 361
              T+K+ +   ++F RW+  NP
Sbjct: 147 -EETLKNYQMVRIIFERWLSWNP 168



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>BCHD_HELMO (Q9ZGE6) Magnesium-chelatase 67 kDa subunit (EC 6.6.1.1)|
           (Mg-protoporphyrin IX chelatase) (Mg-chelatase subunit
           D)
          Length = 666

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 10/16 (62%), Positives = 10/16 (62%)
 Frame = -1

Query: 87  CPCLCNPCEPSIWSLR 40
           CPC CNP EP  W  R
Sbjct: 63  CPCHCNPAEPHSWCNR 78



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>PK3CD_MOUSE (O35904) Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic|
           subunit delta isoform (EC 2.7.1.153) (PI3-kinase p110
           subunit delta) (PtdIns-3-kinase p110) (PI3K) (p110delta)
          Length = 1043

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
 Frame = -1

Query: 117 YGYYRLCHF--LCPCLCNPCEPSIWSLRSWIVMLMKN 13
           YG Y LCHF  +C CL +   P +  + S  ++ M++
Sbjct: 251 YGNYPLCHFQYICSCLHSGLTPHLTMVHSSSILAMRD 287



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>MNT3_CANAL (P87207) Probable mannosyltransferase MNT3 (EC 2.4.1.131)|
          Length = 378

 Score = 28.1 bits (61), Expect = 5.5
 Identities = 13/35 (37%), Positives = 15/35 (42%), Gaps = 10/35 (28%)
 Frame = -1

Query: 120 NYGYYRLCHFLCP----------CLCNPCEPSIWS 46
           N GYY + +  CP          C CNP E   WS
Sbjct: 330 NIGYYHVPYLQCPQDIDLYVKNKCTCNPDEDFTWS 364



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>RIM13_YEAST (Q03792) Calpain-like protease 1 (EC 3.4.22.-) (Cysteine protease|
           RIM13) (Calpain-7) (Regulator of IME2 protein 13)
          Length = 727

 Score = 28.1 bits (61), Expect = 5.5
 Identities = 17/63 (26%), Positives = 29/63 (46%)
 Frame = -1

Query: 264 ELLGK*STNRCSVYSESCF*LEILSKFLLYLVMFGACWYLSFEQLANLNYGYYRLCHFLC 85
           E+ GK S + C+ +    F LE+ S+    ++M  AC   S   L N+   Y+     + 
Sbjct: 464 EIEGKASFDTCNFFQNPTFELEVHSEQDYQVLMDAACISTSSHDLINIQVYYFNDYELIK 523

Query: 84  PCL 76
           P +
Sbjct: 524 PIM 526



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>HMDH_SCHMA (P16237) 3-hydroxy-3-methylglutaryl-coenzyme A reductase (EC|
           1.1.1.34) (HMG-CoA reductase)
          Length = 948

 Score = 27.7 bits (60), Expect = 7.2
 Identities = 12/39 (30%), Positives = 23/39 (58%)
 Frame = +2

Query: 152 QHAPNITKYNRNLDKISNQKQLSEYTLHRFVDYLPRSSS 268
           + +P + K+++ L+ I  Q +   Y LH+  DY+ R+ S
Sbjct: 394 KQSPVLPKFSKKLNDIPLQSRKRIYCLHKDDDYIDRNDS 432



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>RDS_BOVIN (P17810) Peripherin (Retinal degeneration slow protein)|
          Length = 345

 Score = 27.3 bits (59), Expect = 9.4
 Identities = 15/60 (25%), Positives = 27/60 (45%), Gaps = 3/60 (5%)
 Frame = +2

Query: 179 NRNLDKISNQKQLSEYTLHRFVDYLPRSSSIWFKLCRT---*FHSTVKDTEAATVLFRWI 349
           N N  +   Q QL+  + H   D+     ++W + CR     ++S + +T  A  L  W+
Sbjct: 214 NPNSPRPCIQYQLTNNSAHYSYDHQTEELNLWLRGCRAALLSYYSNLMNTTGAVTLLVWL 273



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>SYT_BACTN (Q8AAP2) Threonyl-tRNA synthetase (EC 6.1.1.3) (Threonine--tRNA|
           ligase) (ThrRS)
          Length = 646

 Score = 27.3 bits (59), Expect = 9.4
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = +3

Query: 30  LSTIAMTRWKAHKDYKDMDRENGITY 107
           L+T+A   W+ H+D K + R  GIT+
Sbjct: 197 LTTVAGAYWRGHEDRKMLTRIYGITF 222



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>LEPA_TREDE (P60794) GTP-binding protein lepA|
          Length = 601

 Score = 27.3 bits (59), Expect = 9.4
 Identities = 13/39 (33%), Positives = 18/39 (46%)
 Frame = +3

Query: 69  DYKDMDRENGITYSSHSSNLPTVQNSSTNMPQT*LSTTG 185
           D  D++RE GIT  SH+  +P       N     + T G
Sbjct: 45  DDMDIERERGITIKSHAVRIPYTAKDGKNYVLNFVDTPG 83


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,079,636
Number of Sequences: 219361
Number of extensions: 995580
Number of successful extensions: 2804
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2709
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2804
length of database: 80,573,946
effective HSP length: 96
effective length of database: 59,515,290
effective search space used: 1428366960
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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