ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart25b10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RK22_HUPLU (Q5SD20) Chloroplast 50S ribosomal protein L22 30 1.0
2ATG15_ASPFU (Q4X180) Putative lipase atg15 (EC 3.1.1.3) (Autopha... 30 1.8
3LAMA5_HUMAN (O15230) Laminin alpha-5 chain precursor 29 3.0
4LIMK1_DROME (Q8IR79) LIM domain kinase 1 (EC 2.7.11.1) (LIMK-1) ... 28 5.2
5ATG1_EMENI (Q5BCU8) Serine/threonine-protein kinase atg1 (EC 2.7... 28 5.2
6TBK1_XENLA (Q6DFJ6) Serine/threonine-protein kinase TBK1 (EC 2.7... 27 8.9
7ATG2_CRYNE (Q5KMS0) Autophagy-related protein 2 27 8.9
8Y3499_BACCR (Q813A5) UPF0061 protein BC_3499 27 8.9
9RPOB_STRPM (Q48VR1) DNA-directed RNA polymerase beta chain (EC 2... 27 8.9
10RPOB_STRP8 (Q8P2Y3) DNA-directed RNA polymerase beta chain (EC 2... 27 8.9
11RPOB_STRP6 (Q5XE97) DNA-directed RNA polymerase beta chain (EC 2... 27 8.9
12RPOB_STRP3 (Q8K8W3) DNA-directed RNA polymerase beta chain (EC 2... 27 8.9
13RPOB_STRP1 (Q9A1U1) DNA-directed RNA polymerase beta chain (EC 2... 27 8.9
14ACP_BRACM (P07088) Acyl carrier protein SF2, chloroplast precurs... 27 8.9
15ACP3_BRANA (P32887) Acyl carrier protein, chloroplast precursor ... 27 8.9
16Y481_TREPA (O83494) Hypothetical protein TP0481 27 8.9

>RK22_HUPLU (Q5SD20) Chloroplast 50S ribosomal protein L22|
          Length = 125

 Score = 30.4 bits (67), Expect = 1.0
 Identities = 14/26 (53%), Positives = 16/26 (61%)
 Frame = -3

Query: 148 ASSIAKSLRLRGVGRTVP*YKPFCKL 71
           A S AK LRLR  GR  P +KP C +
Sbjct: 86  AGSFAKRLRLRAQGRNYPIHKPTCHI 111



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>ATG15_ASPFU (Q4X180) Putative lipase atg15 (EC 3.1.1.3) (Autophagy-related|
           protein 15)
          Length = 650

 Score = 29.6 bits (65), Expect = 1.8
 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 1/57 (1%)
 Frame = +1

Query: 241 WFPAPAATSTTNTLMSFRTTHSLPRRVTIATASRA-CELPRWFPLEAQPLHESAASS 408
           WF     T+TT    +   T S+P   T  T+S + C+ P WF     P   ++ +S
Sbjct: 528 WFGCKDPTTTTEATAAPSVTTSIPTPTTYPTSSTSTCKDPGWFGCRDPPSTTASITS 584



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>LAMA5_HUMAN (O15230) Laminin alpha-5 chain precursor|
          Length = 3695

 Score = 28.9 bits (63), Expect = 3.0
 Identities = 29/90 (32%), Positives = 39/90 (43%), Gaps = 10/90 (11%)
 Frame = +3

Query: 159  PAAAGNACQEVRDEALYLSFAGEGL----PPLVSCPGGDVDDQHAHVVSNNTQLAAKSDH 326
            P+AAG      +D +L      E L    PPL++C G DVD Q    V    + A   ++
Sbjct: 1056 PSAAGLEALCRQDNSLPRPCPTEQLSPSHPPLITCTGSDVDVQLQVAVPQPGRYALVVEY 1115

Query: 327  CN---RLE---SV*TPQVVPA*GTATPRVC 398
             N   R E   +V TPQ  P  G  +   C
Sbjct: 1116 ANEDARQEVGVAVHTPQRAPQQGLLSLHPC 1145



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>LIMK1_DROME (Q8IR79) LIM domain kinase 1 (EC 2.7.11.1) (LIMK-1) (dLIMK)|
          Length = 1257

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 16/43 (37%), Positives = 20/43 (46%)
 Frame = +1

Query: 214  PLPARVSHRWFPAPAATSTTNTLMSFRTTHSLPRRVTIATASR 342
            PLP+R   +  PA    S T +      T  L +R T ATA R
Sbjct: 1109 PLPSRTGSQGIPASNCVSPTRSSRPGSPTKHLAQRHTAATAQR 1151



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>ATG1_EMENI (Q5BCU8) Serine/threonine-protein kinase atg1 (EC 2.7.11.1)|
           (Autophagy-related protein 1)
          Length = 935

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 21/67 (31%), Positives = 31/67 (46%)
 Frame = -3

Query: 271 SSTSPPGQETNGGRPSPAKDR*RASSRTSWQALPAAAGPKTASSIAKSLRLRGVGRTVP* 92
           +S+SP  +  +G RPS AK    A++    +A+  A  P     I +    +    TVP 
Sbjct: 401 TSSSPMRRTRSGDRPSSAKQIPPAAAPPRPEAVSHATAPGRQELIGRHSSEQQAPGTVPV 460

Query: 91  YKPFCKL 71
           YK   KL
Sbjct: 461 YKNVDKL 467



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>TBK1_XENLA (Q6DFJ6) Serine/threonine-protein kinase TBK1 (EC 2.7.11.1)|
          Length = 725

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 25/81 (30%), Positives = 37/81 (45%), Gaps = 3/81 (3%)
 Frame = +3

Query: 102 VRPTPLSLKDLAMEDAVFGPAAAGNACQEVRDEA---LYLSFAGEGLPPLVSCPGGDVDD 272
           V P P+S  DL + DA    A  G AC   R  A   L L    +G+  L       + +
Sbjct: 398 VIPKPISHYDLDV-DANMAKAVTGVACYYCRIAASLLLVLDLMRKGIRWLSEL----MKE 452

Query: 273 QHAHVVSNNTQLAAKSDHCNR 335
           ++   V  NT+++ K + CNR
Sbjct: 453 EYNENVHRNTEVSLKLNFCNR 473



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>ATG2_CRYNE (Q5KMS0) Autophagy-related protein 2|
          Length = 1935

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 14/30 (46%), Positives = 16/30 (53%), Gaps = 2/30 (6%)
 Frame = -3

Query: 196  SRTSWQALPA--AAGPKTASSIAKSLRLRG 113
            S +SWQ LPA   AGP       K  RL+G
Sbjct: 1474 SGSSWQTLPAGVGAGPSAVHQAHKKTRLKG 1503



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>Y3499_BACCR (Q813A5) UPF0061 protein BC_3499|
          Length = 488

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 16/49 (32%), Positives = 25/49 (51%)
 Frame = +3

Query: 90  YYGTVRPTPLSLKDLAMEDAVFGPAAAGNACQEVRDEALYLSFAGEGLP 236
           +Y  + PTP+S  +L   +      + G   +E++ EA    FAG GLP
Sbjct: 22  FYTEIPPTPVSSPELVKLNHSLA-ISLGLTPEELKKEAEIAIFAGNGLP 69



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>RPOB_STRPM (Q48VR1) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (RNAP|
           beta subunit) (Transcriptase beta chain) (RNA polymerase
           beta subunit)
          Length = 1188

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = +3

Query: 111 TPLSLKDLAMEDAVFGPAAAGNACQEVRDEALYLSFAGEGL 233
           TP   KDL+ E+ +   A  G+  +EVRD +L +   G+G+
Sbjct: 843 TPKGEKDLSAEERLLH-AIFGDKSREVRDTSLRVPHGGDGI 882



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>RPOB_STRP8 (Q8P2Y3) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (RNAP|
           beta subunit) (Transcriptase beta chain) (RNA polymerase
           beta subunit)
          Length = 1188

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = +3

Query: 111 TPLSLKDLAMEDAVFGPAAAGNACQEVRDEALYLSFAGEGL 233
           TP   KDL+ E+ +   A  G+  +EVRD +L +   G+G+
Sbjct: 843 TPKGEKDLSAEERLLH-AIFGDKSREVRDTSLRVPHGGDGI 882



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>RPOB_STRP6 (Q5XE97) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (RNAP|
           beta subunit) (Transcriptase beta chain) (RNA polymerase
           beta subunit)
          Length = 1188

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = +3

Query: 111 TPLSLKDLAMEDAVFGPAAAGNACQEVRDEALYLSFAGEGL 233
           TP   KDL+ E+ +   A  G+  +EVRD +L +   G+G+
Sbjct: 843 TPKGEKDLSAEERLLH-AIFGDKSREVRDTSLRVPHGGDGI 882



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>RPOB_STRP3 (Q8K8W3) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (RNAP|
           beta subunit) (Transcriptase beta chain) (RNA polymerase
           beta subunit)
          Length = 1188

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = +3

Query: 111 TPLSLKDLAMEDAVFGPAAAGNACQEVRDEALYLSFAGEGL 233
           TP   KDL+ E+ +   A  G+  +EVRD +L +   G+G+
Sbjct: 843 TPKGEKDLSAEERLLH-AIFGDKSREVRDTSLRVPHGGDGI 882



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>RPOB_STRP1 (Q9A1U1) DNA-directed RNA polymerase beta chain (EC 2.7.7.6) (RNAP|
           beta subunit) (Transcriptase beta chain) (RNA polymerase
           beta subunit)
          Length = 1188

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = +3

Query: 111 TPLSLKDLAMEDAVFGPAAAGNACQEVRDEALYLSFAGEGL 233
           TP   KDL+ E+ +   A  G+  +EVRD +L +   G+G+
Sbjct: 843 TPKGEKDLSAEERLLH-AIFGDKSREVRDTSLRVPHGGDGI 882



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>ACP_BRACM (P07088) Acyl carrier protein SF2, chloroplast precursor (ACP)|
          Length = 134

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 14/33 (42%), Positives = 20/33 (60%)
 Frame = +1

Query: 244 FPAPAATSTTNTLMSFRTTHSLPRRVTIATASR 342
           F  PA  STTN  +SF    S+P R +I+ A++
Sbjct: 24  FQKPALVSTTN--LSFNLRRSIPTRFSISCAAK 54



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>ACP3_BRANA (P32887) Acyl carrier protein, chloroplast precursor (ACP) (Clones|
           34C02 and 10C04)
          Length = 134

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 15/41 (36%), Positives = 24/41 (58%)
 Frame = +1

Query: 220 PARVSHRWFPAPAATSTTNTLMSFRTTHSLPRRVTIATASR 342
           P R+S   F  PA  S TN  +SF    S+P R++++ A++
Sbjct: 19  PTRIS---FQKPALVSRTN--LSFNLRRSIPTRLSVSCAAK 54



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>Y481_TREPA (O83494) Hypothetical protein TP0481|
          Length = 477

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 14/32 (43%), Positives = 17/32 (53%)
 Frame = -3

Query: 205 RASSRTSWQALPAAAGPKTASSIAKSLRLRGV 110
           R S RT     P+A   KTA S+    RL+GV
Sbjct: 434 RGSQRTPSDGSPSAEAEKTAQSLTTRKRLQGV 465


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,766,375
Number of Sequences: 219361
Number of extensions: 1154260
Number of successful extensions: 3623
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 3514
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3622
length of database: 80,573,946
effective HSP length: 111
effective length of database: 56,224,875
effective search space used: 1349397000
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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