ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart24g03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RETO_ESCCA (P30986) Reticuline oxidase precursor (EC 1.21.3.3) (... 105 9e-23
2RETO_PAPSO (P93479) Reticuline oxidase precursor (EC 1.21.3.3) (... 104 1e-22
3MCRA_STRLA (P43485) Mitomycin radical oxidase (EC 1.5.3.-) 32 1.2
4FUSB_BURCE (P24127) Fusaric acid resistance protein fusB 32 1.2
5YXXB_BACSU (P39139) Hypothetical protein yxxB 30 2.7
6ZNFX1_MOUSE (Q8R151) NFX1-type zinc finger-containing protein 1 30 3.5
7HELS_PYRAB (Q9V0A9) Putative ski2-type helicase (EC 3.6.1.-) 29 6.0
8GLPK_MOUSE (Q64516) Glycerol kinase (EC 2.7.1.30) (ATP:glycerol ... 29 6.0
9CATB_CHICK (P43233) Cathepsin B precursor (EC 3.4.22.1) (Catheps... 29 6.0
10BXB_CLOBO (P10844) Botulinum neurotoxin type B precursor (EC 3.4... 29 7.8
11HDNO_ARTOX (P08159) 6-hydroxy-D-nicotine oxidase (EC 1.5.3.6) (6... 29 7.8
12BGAL_LACDE (P20043) Beta-galactosidase (EC 3.2.1.23) (Lactase) 29 7.8
13Y703_ENCCU (Q8ST93) Hypothetical protein ECU07_0030/ECU09_2040 29 7.8

>RETO_ESCCA (P30986) Reticuline oxidase precursor (EC 1.21.3.3)|
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase)
          Length = 538

 Score =  105 bits (261), Expect = 9e-23
 Identities = 54/128 (42%), Positives = 76/128 (59%), Gaps = 8/128 (6%)
 Frame = -3

Query: 493 GFMGSVPTDATPYPHRSGVLYNVQYMVFW---QGDGGTAANTWLGNLYDFMGQYVSKNPR 323
           G M  + +D TP+PHRSG    V+Y+V W   +    T    WL  +Y+FM  +VSKNPR
Sbjct: 394 GQMSKISSDFTPFPHRSGTRLMVEYIVAWNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPR 453

Query: 322 QAYVNYRDLDIG-----QNVVVDDATTFDSAKVWGEQYFTSNFQRLAAVKAAVDPTDYFR 158
             YVN+ DLD+G        VV++A   + ++ WGE YF SN++RL   K  +DP + F 
Sbjct: 454 LGYVNHIDLDLGGIDWGNKTVVNNA--IEISRSWGESYFLSNYERLIRAKTLIDPNNVFN 511

Query: 157 NEQSIPPL 134
           + QSIPP+
Sbjct: 512 HPQSIPPM 519



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>RETO_PAPSO (P93479) Reticuline oxidase precursor (EC 1.21.3.3)|
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase)
          Length = 535

 Score =  104 bits (259), Expect = 1e-22
 Identities = 49/128 (38%), Positives = 77/128 (60%), Gaps = 6/128 (4%)
 Frame = -3

Query: 493 GFMGSVPTDATPYPHRSGVLYNVQYMVFWQGDGGTAA---NTWLGNLYDFMGQYVSKNPR 323
           G M  + TD TP+PHR G     +Y++ W  D  +     + WL   YD++  +VSK PR
Sbjct: 398 GKMSEISTDFTPFPHRKGTKLMFEYIIAWNQDEESKIGEFSEWLAKFYDYLEPFVSKEPR 457

Query: 322 QAYVNYRDLDIGQNVVVDDATTFDSAKV---WGEQYFTSNFQRLAAVKAAVDPTDYFRNE 152
             YVN+ DLDIG     + ++T ++ ++   WGE+YF+SN++RL   K  +DP + F + 
Sbjct: 458 VGYVNHIDLDIGGIDWRNKSSTTNAVEIARNWGERYFSSNYERLVKAKTLIDPNNVFNHP 517

Query: 151 QSIPPLVQ 128
           QSIPP+++
Sbjct: 518 QSIPPMMK 525



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>MCRA_STRLA (P43485) Mitomycin radical oxidase (EC 1.5.3.-)|
          Length = 447

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 15/30 (50%), Positives = 19/30 (63%)
 Frame = -3

Query: 226 YFTSNFQRLAAVKAAVDPTDYFRNEQSIPP 137
           Y  ++F RL AVKA  DP + FR   +IPP
Sbjct: 413 YTPADFARLRAVKAQYDPDNMFRVNFNIPP 442



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>FUSB_BURCE (P24127) Fusaric acid resistance protein fusB|
          Length = 142

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 15/40 (37%), Positives = 22/40 (55%), Gaps = 3/40 (7%)
 Frame = +1

Query: 325 SGSCSRTAP*S---RTSCRARCSPPCPRHPARTPCTGRCT 435
           S  CSR++      R+ C +RC+PP PR  A +  T  C+
Sbjct: 97  SSGCSRSSRSCSCWRSPCGSRCAPPAPRATATSAVTASCS 136



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>YXXB_BACSU (P39139) Hypothetical protein yxxB|
          Length = 289

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 18/70 (25%), Positives = 33/70 (47%), Gaps = 1/70 (1%)
 Frame = -3

Query: 445 SGVLYNVQYMVFWQGDGGTAANTWLGN-LYDFMGQYVSKNPRQAYVNYRDLDIGQNVVVD 269
           + + +N    VF QGDGGT+  TW    L    GQ+++      ++ +  +   + V   
Sbjct: 117 TSIAWNALRFVFGQGDGGTSEQTWSAYVLAQPFGQWLTGLTGAGFIVFAIVQFMKGVRAA 176

Query: 268 DATTFDSAKV 239
               FD++K+
Sbjct: 177 FMKEFDTSKM 186



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>ZNFX1_MOUSE (Q8R151) NFX1-type zinc finger-containing protein 1|
          Length = 1909

 Score = 30.0 bits (66), Expect = 3.5
 Identities = 16/43 (37%), Positives = 17/43 (39%), Gaps = 10/43 (23%)
 Frame = +1

Query: 334  CSRTAP*SRTSC-----RARCSPPCPR-----HPARTPCTGRC 432
            C    P S  SC       RC  PC R     H  + PCTG C
Sbjct: 1433 CGHPCPGSCHSCFEGRFHERCQQPCKRLLICSHKCQEPCTGEC 1475



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>HELS_PYRAB (Q9V0A9) Putative ski2-type helicase (EC 3.6.1.-)|
          Length = 715

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 15/41 (36%), Positives = 20/41 (48%)
 Frame = -3

Query: 478 VPTDATPYPHRSGVLYNVQYMVFWQGDGGTAANTWLGNLYD 356
           + +D  P   R GV Y  Q  VFW+  G    N+W   +YD
Sbjct: 194 IRSDWRPVKLRKGVFY--QGFVFWEDGGSERYNSWEELVYD 232



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>GLPK_MOUSE (Q64516) Glycerol kinase (EC 2.7.1.30) (ATP:glycerol|
           3-phosphotransferase) (Glycerokinase) (GK)
          Length = 524

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +2

Query: 143 DALLVSEVVGGIHGRLHCREPLEVAREVL 229
           D+ L+  + GGIHG +HC +    +R +L
Sbjct: 179 DSWLIWSLTGGIHGGVHCTDVTNASRTML 207



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>CATB_CHICK (P43233) Cathepsin B precursor (EC 3.4.22.1) (Cathepsin B1)|
           [Contains: Cathepsin B light chain; Cathepsin B heavy
           chain]
          Length = 340

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 13/26 (50%), Positives = 14/26 (53%), Gaps = 2/26 (7%)
 Frame = +1

Query: 355 SRTSCRARCSPPCPRH--PARTPCTG 426
           S   CRA   PPC  H   +R PCTG
Sbjct: 175 SHVGCRAYTIPPCEHHVNGSRPPCTG 200



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>BXB_CLOBO (P10844) Botulinum neurotoxin type B precursor (EC 3.4.24.69)|
            (BoNT/B) (Bontoxilysin B) [Contains: Botulinum neurotoxin
            B light chain; Botulinum neurotoxin B heavy chain]
          Length = 1290

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 17/60 (28%), Positives = 34/60 (56%), Gaps = 4/60 (6%)
 Frame = -3

Query: 439  VLYNVQYMVFWQGDGGTAANTWLGNLYDF-MGQYVSK---NPRQAYVNYRDLDIGQNVVV 272
            ++YN +Y +F  G+     N+++    D  +G+ +++   N    Y+NYRDL IG+  ++
Sbjct: 1091 LMYNKEYYMFNAGN----KNSYIKLKKDSPVGEILTRSKYNQNSKYINYRDLYIGEKFII 1146



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>HDNO_ARTOX (P08159) 6-hydroxy-D-nicotine oxidase (EC 1.5.3.6) (6-HDNO)|
          Length = 458

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 15/38 (39%), Positives = 22/38 (57%)
 Frame = -3

Query: 250 SAKVWGEQYFTSNFQRLAAVKAAVDPTDYFRNEQSIPP 137
           +A++  E Y    + RLAAVK   DP + FR+  +I P
Sbjct: 418 TAEMVAEVYKPEVYCRLAAVKREYDPENRFRHNYNIDP 455



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>BGAL_LACDE (P20043) Beta-galactosidase (EC 3.2.1.23) (Lactase)|
          Length = 1006

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 17/46 (36%), Positives = 21/46 (45%)
 Frame = -3

Query: 430 NVQYMVFWQGDGGTAANTWLGNLYDFMGQYVSKNPRQAYVNYRDLD 293
           N++   F Q         W G+   F  Q  SKNP  +YV Y DLD
Sbjct: 91  NLELQGFGQPQYVNVQYPWDGSEEIFPPQIPSKNPLASYVRYFDLD 136



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>Y703_ENCCU (Q8ST93) Hypothetical protein ECU07_0030/ECU09_2040|
          Length = 236

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 15/34 (44%), Positives = 17/34 (50%), Gaps = 2/34 (5%)
 Frame = +1

Query: 322 ASGSCSRTA--P*SRTSCRARCSPPCPRHPARTP 417
           AS    RTA  P  R SC+ +   PC  HP R P
Sbjct: 82  ASNRAHRTAKHPARRQSCKLKAPNPCVGHPLRIP 115


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,771,455
Number of Sequences: 219361
Number of extensions: 1423414
Number of successful extensions: 4688
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 4474
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4679
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3465624120
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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