ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart23e06
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor ... 33 0.13
2PEPC_CAVPO (Q64411) Gastricsin precursor (EC 3.4.23.3) (Pepsinog... 32 0.48
3CIC_DROME (Q9U1H0) Putative transcription factor capicua (Protei... 31 0.63
4SYF1_YEAST (Q04048) Pre-mRNA-splicing factor SYF1 (Synthetic let... 31 0.82
5ASPRX_ORYSA (P42211) Aspartic proteinase precursor (EC 3.4.23.-) 30 1.1
6NEP2_NEPGR (Q766C2) Aspartic proteinase nepenthesin-2 precursor ... 30 1.4
7COBT_RHIME (Q92P98) Nicotinate-nucleotide--dimethylbenzimidazole... 30 1.4
8ARGB_SYNEL (P59303) Acetylglutamate kinase (EC 2.7.2.8) (NAG kin... 29 2.4
9CU030_HUMAN (Q9UFM2) Putative protein C21orf30 29 3.1
10YKC2_CAEEL (P41993) Hypothetical protein B0280.2 29 3.1
11REP_BUCAP (O51889) ATP-dependent DNA helicase rep (EC 3.6.1.-) 28 4.1
12NACH_DROAN (O61370) Sodium channel protein Nach 28 4.1
13VE2_HPV69 (Q9JH48) Regulatory protein E2 28 5.3
14LIMD1_HUMAN (Q9UGP4) LIM domains-containing protein 1 28 5.3
15HAIR_HUMAN (O43593) Protein hairless 28 5.3
16ARFG1_RAT (Q62848) ADP-ribosylation factor GTPase-activating pro... 28 6.9
17PEPC_SORUN (Q9GMY4) Gastricsin precursor (EC 3.4.23.3) (Pepsinog... 28 6.9
18CRLF2_MOUSE (Q8CII9) Cytokine receptor-like factor 2 precursor (... 28 6.9
19ARFG1_MOUSE (Q9EPJ9) ADP-ribosylation factor GTPase-activating p... 28 6.9
20YG3K_YEAST (P48235) Hypothetical 24.0 kDa protein in MOL1-NAT2 i... 28 6.9
21SDC1_CAEEL (P24349) Zinc finger protein sdc-1 (Egg-laying defect... 27 9.0
22PEPC_HUMAN (P20142) Gastricsin precursor (EC 3.4.23.3) (Pepsinog... 27 9.0
23ARD3_ORYSA (Q7XNS7) Putative 1,2-dihydroxy-3-keto-5-methylthiope... 27 9.0
24CRDL1_RAT (Q76LD0) Chordin-like protein 1 precursor (Kohjirin) 27 9.0
25CYB_TRYBB (P00164) Cytochrome b 27 9.0

>NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor (EC 3.4.23.-)|
           (Nepenthesin-I)
          Length = 437

 Score = 33.5 bits (75), Expect = 0.13
 Identities = 14/44 (31%), Positives = 20/44 (45%)
 Frame = -2

Query: 353 DGCLAFAATPGEESVGFIGNVQQQTYEVLYDVGGGKVGFRRGAC 222
           +G +  A     + +   GN+QQQ   V+YD G   V F    C
Sbjct: 391 NGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434



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>PEPC_CAVPO (Q64411) Gastricsin precursor (EC 3.4.23.3) (Pepsinogen C)|
          Length = 394

 Score = 31.6 bits (70), Expect = 0.48
 Identities = 19/64 (29%), Positives = 31/64 (48%), Gaps = 8/64 (12%)
 Frame = -2

Query: 392 GATLDLEPASIILDG---CLA-----FAATPGEESVGFIGNVQQQTYEVLYDVGGGKVGF 237
           G    L P++ IL G   C+      + +  G E V  +G+V  ++Y  +YD+   +VGF
Sbjct: 331 GVEFPLSPSAYILSGENYCMVGLESTYVSPGGGEPVWILGDVFLRSYYSVYDLANNRVGF 390

Query: 236 RRGA 225
              A
Sbjct: 391 ATAA 394



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>CIC_DROME (Q9U1H0) Putative transcription factor capicua (Protein fettucine)|
          Length = 1403

 Score = 31.2 bits (69), Expect = 0.63
 Identities = 19/58 (32%), Positives = 26/58 (44%)
 Frame = +1

Query: 217  HQHAPRRNPTLPPPTSYRTS*VCCCTLPMNPTDSSPGVAANARQPSKIMEAGSRSKVA 390
            H ++   +  LP PTS  T+      LP+ PT SS     N +QP   M+    S  A
Sbjct: 1116 HVNSSNLSSALPTPTSSTTTPNSDEQLPLTPTTSSSNSNLNQQQPKSPMKGAPGSTAA 1173



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>SYF1_YEAST (Q04048) Pre-mRNA-splicing factor SYF1 (Synthetic lethal with CDC40|
           protein 1) (PRP19-associated complex protein 90)
          Length = 859

 Score = 30.8 bits (68), Expect = 0.82
 Identities = 18/47 (38%), Positives = 24/47 (51%), Gaps = 3/47 (6%)
 Frame = +2

Query: 260 RRTELRKSAAARCR*TRRILHRGWPR---TPGSHPKLWKPVRDPRWR 391
           +R  L+KSAA +C      + +  PR   TPGS  +LW    D  WR
Sbjct: 413 KRVSLQKSAAEKCNVYSEAILKIDPRKVGTPGSFGRLWCSYGDLYWR 459



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>ASPRX_ORYSA (P42211) Aspartic proteinase precursor (EC 3.4.23.-)|
          Length = 496

 Score = 30.4 bits (67), Expect = 1.1
 Identities = 12/47 (25%), Positives = 24/47 (51%)
 Frame = -2

Query: 365 SIILDGCLAFAATPGEESVGFIGNVQQQTYEVLYDVGGGKVGFRRGA 225
           ++ + G +AF   P    +  +G+V    Y  ++D G  ++GF + A
Sbjct: 450 TVCISGFMAFDIPPPRGPLWILGDVFMGAYHTVFDFGKDRIGFAKSA 496



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>NEP2_NEPGR (Q766C2) Aspartic proteinase nepenthesin-2 precursor (EC 3.4.23.-)|
           (Nepenthesin-II)
          Length = 438

 Score = 30.0 bits (66), Expect = 1.4
 Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 5/62 (8%)
 Frame = -2

Query: 392 GATLDLEPASIILDG-----CLAFAATPGEESVGFIGNVQQQTYEVLYDVGGGKVGFRRG 228
           G  L+L   +I++       CLA  ++  +  +   GN+QQQ  +VLYD+    V F   
Sbjct: 375 GGVLNLGEQNILISPAEGVICLAMGSS-SQLGISIFGNIQQQETQVLYDLQNLAVSFVPT 433

Query: 227 AC 222
            C
Sbjct: 434 QC 435



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>COBT_RHIME (Q92P98) Nicotinate-nucleotide--dimethylbenzimidazole|
           phosphoribosyltransferase (EC 2.4.2.21) (NN:DBI PRT)
           (N(1)-alpha-phosphoribosyltransferase)
          Length = 338

 Score = 30.0 bits (66), Expect = 1.4
 Identities = 10/16 (62%), Positives = 11/16 (68%)
 Frame = -3

Query: 358 FWMAAWRSRPPPVKNP 311
           FW+AAW  RPP V  P
Sbjct: 51  FWLAAWTGRPPAVNRP 66



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>ARGB_SYNEL (P59303) Acetylglutamate kinase (EC 2.7.2.8) (NAG kinase) (AGK)|
           (N-acetyl-L-glutamate 5-phosphotransferase)
          Length = 295

 Score = 29.3 bits (64), Expect = 2.4
 Identities = 13/22 (59%), Positives = 15/22 (68%)
 Frame = -2

Query: 353 DGCLAFAATPGEESVGFIGNVQ 288
           DG L  A   GEES+GF+G VQ
Sbjct: 126 DGNLIRARAKGEESIGFVGEVQ 147



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>CU030_HUMAN (Q9UFM2) Putative protein C21orf30|
          Length = 244

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 18/55 (32%), Positives = 26/55 (47%), Gaps = 7/55 (12%)
 Frame = -3

Query: 343 WRSRP-----PPVKNPSG--SSATCSSRLTKFCTTSAVARWDSVVERADVAGCRT 200
           WRS P     P  ++P+    S  CS  ++  CT    ARWD+  + A +  C T
Sbjct: 108 WRSTPEMTSLPAPEHPASPCDSVLCSPDVS-MCTLGPAARWDAQAKSAPLPPCCT 161



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>YKC2_CAEEL (P41993) Hypothetical protein B0280.2|
          Length = 627

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 22/72 (30%), Positives = 31/72 (43%), Gaps = 3/72 (4%)
 Frame = +1

Query: 184 PSHDWLSCNLPHQHAPRRNPTLPPPTSYRTS*VCCCTLPMNPTDSSPGVAAN---ARQPS 354
           P+H   S   P   AP R P  P P    ++ +C       PT S+P    N   A  P+
Sbjct: 386 PNHTHCS-EKPIAEAPLRPPVRPAPIVPTSAGLCLVLAASFPTSSTPSRFLNPFPAPLPA 444

Query: 355 KIMEAGSRSKVA 390
           +     S+SK+A
Sbjct: 445 ESFFGNSKSKIA 456



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>REP_BUCAP (O51889) ATP-dependent DNA helicase rep (EC 3.6.1.-)|
          Length = 658

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 13/39 (33%), Positives = 26/39 (66%), Gaps = 2/39 (5%)
 Frame = +1

Query: 55  VRITIIGHLKKNYTYYI--FFFHLFITN*LDHNELACIP 165
           V+++   +L+K++ ++   + FHL  +N LD ++L CIP
Sbjct: 152 VQLSAQSNLEKDFAFFYKQYTFHLRKSNILDFDDLICIP 190



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>NACH_DROAN (O61370) Sodium channel protein Nach|
          Length = 516

 Score = 28.5 bits (62), Expect = 4.1
 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 4/38 (10%)
 Frame = +2

Query: 272 LRKSAAARCR*TRRILHRG----WPRTPGSHPKLWKPV 373
           LRK+    C   +++LH+G    WP+   SH K  K V
Sbjct: 473 LRKNYVLECEARKKMLHKGPKFAWPKANDSHSKHQKSV 510



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>VE2_HPV69 (Q9JH48) Regulatory protein E2|
          Length = 368

 Score = 28.1 bits (61), Expect = 5.3
 Identities = 13/38 (34%), Positives = 19/38 (50%), Gaps = 2/38 (5%)
 Frame = -3

Query: 247 RWDSVVERADVAGCRTTSRV--MAQWYVFGVCKLAHCD 140
           RW SV  + D+  C+T   V     +Y+ G CK  + D
Sbjct: 133 RWTSVYYKTDMGWCKTNGEVDYKGIYYMHGCCKQYYVD 170



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>LIMD1_HUMAN (Q9UGP4) LIM domains-containing protein 1|
          Length = 676

 Score = 28.1 bits (61), Expect = 5.3
 Identities = 11/18 (61%), Positives = 12/18 (66%)
 Frame = +2

Query: 326 GWPRTPGSHPKLWKPVRD 379
           GWP +PGS P L KP  D
Sbjct: 207 GWPSSPGSDPPLPKPCGD 224



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>HAIR_HUMAN (O43593) Protein hairless|
          Length = 1189

 Score = 28.1 bits (61), Expect = 5.3
 Identities = 29/96 (30%), Positives = 39/96 (40%), Gaps = 16/96 (16%)
 Frame = +1

Query: 154 ACIPQ-IHTIGPSHDWLSC---NLPHQHAPRRNPTLPPPTSYRTS*VCCCTLPMNPT--- 312
           AC P  +HT+G  + W      NL +Q  P   P  P P    T   CC + P  PT   
Sbjct: 281 ACPPGLVHTLG--NVWAGPGDGNLGYQLGPPATPRCPSPEPPVTQRGCCSSYP--PTKGG 336

Query: 313 DSSP---------GVAANARQPSKIMEAGSRSKVAP 393
           D  P         G A+ A +PS+ +   S  +  P
Sbjct: 337 DLGPCGKCQEGLEGGASGASEPSEEVNKASGPRACP 372



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>ARFG1_RAT (Q62848) ADP-ribosylation factor GTPase-activating protein 1|
           (ADP-ribosylation factor 1 GTPase-activating protein)
           (ARF1 GAP) (ARF1-directed GTPase-activating protein)
           (GAP protein)
          Length = 415

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 13/41 (31%), Positives = 19/41 (46%)
 Frame = -3

Query: 358 FWMAAWRSRPPPVKNPSGSSATCSSRLTKFCTTSAVARWDS 236
           FW     + PP  K+PS  S TC+   T   ++ +   W S
Sbjct: 328 FWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDIWGS 368



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>PEPC_SORUN (Q9GMY4) Gastricsin precursor (EC 3.4.23.3) (Pepsinogen C)|
          Length = 389

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 15/54 (27%), Positives = 27/54 (50%)
 Frame = -2

Query: 386 TLDLEPASIILDGCLAFAATPGEESVGFIGNVQQQTYEVLYDVGGGKVGFRRGA 225
           TL +EP          +  +P  + +  +G+V  ++Y  +YD+G  +VGF   A
Sbjct: 345 TLGVEPT---------YLPSPTGQPLWILGDVFLRSYYSVYDMGNNRVGFATAA 389



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>CRLF2_MOUSE (Q8CII9) Cytokine receptor-like factor 2 precursor (Type I cytokine|
           receptor delta 1) (Cytokine receptor-like molecule 2)
           (CRLM-2) (Thymic stromal lymphopoietin protein receptor)
           (TSLPR)
          Length = 359

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 14/35 (40%), Positives = 16/35 (45%), Gaps = 1/35 (2%)
 Frame = -1

Query: 264 RRRRWQGGIPSWSV-LMWQVAGQPVV*WPNGMYLG 163
           R   W    P W+V L+W   G   V WP   YLG
Sbjct: 110 RASAWLKPRPPWNVTLLWTPDGDVTVSWPAHSYLG 144



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>ARFG1_MOUSE (Q9EPJ9) ADP-ribosylation factor GTPase-activating protein 1|
           (ADP-ribosylation factor 1 GTPase-activating protein)
           (ARF1 GAP) (ARF1-directed GTPase-activating protein)
           (GAP protein)
          Length = 414

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 13/41 (31%), Positives = 19/41 (46%)
 Frame = -3

Query: 358 FWMAAWRSRPPPVKNPSGSSATCSSRLTKFCTTSAVARWDS 236
           FW     + PP  K+PS  S TC+   T   ++ +   W S
Sbjct: 328 FWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDVWGS 368



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>YG3K_YEAST (P48235) Hypothetical 24.0 kDa protein in MOL1-NAT2 intergenic|
           region
          Length = 211

 Score = 27.7 bits (60), Expect = 6.9
 Identities = 22/87 (25%), Positives = 36/87 (41%)
 Frame = +3

Query: 30  NSHWLDVPCKDYNHRPFEKKLHVLHLLLPFIYNKLARSQ*ASLHTPNTYHWAITRLVVLQ 209
           N  +    CK  +  P  +KL      L  ++ K   S   +L  P +Y   +T   + +
Sbjct: 43  NKLYCSEDCKLKDSNPLNEKL------LSHLHKKSKTSHSHNLTPPLSYSKNLTASNLFE 96

Query: 210 PATSARSTTESHLATADVVQNFVSLLL 290
           P TS  S+  S     D ++   SLL+
Sbjct: 97  PTTSLSSSPTSSTIPFDELEKLESLLI 123



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>SDC1_CAEEL (P24349) Zinc finger protein sdc-1 (Egg-laying defective protein|
           16)
          Length = 1203

 Score = 27.3 bits (59), Expect = 9.0
 Identities = 16/46 (34%), Positives = 21/46 (45%), Gaps = 6/46 (13%)
 Frame = +2

Query: 200 CPATCHISTLHDGIPPCH---RRRR---TELRKSAAARCR*TRRIL 319
           C   CH + LHD +  CH   R +R    E  K     CR T R++
Sbjct: 659 CGTRCHYTLLHDHLDYCHYWPRNKRLVNEEFHKWKKNGCRNTWRVM 704



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>PEPC_HUMAN (P20142) Gastricsin precursor (EC 3.4.23.3) (Pepsinogen C)|
          Length = 388

 Score = 27.3 bits (59), Expect = 9.0
 Identities = 17/64 (26%), Positives = 30/64 (46%), Gaps = 8/64 (12%)
 Frame = -2

Query: 392 GATLDLEPASIILDG---CLA-----FAATPGEESVGFIGNVQQQTYEVLYDVGGGKVGF 237
           G    L P+S IL     C       + ++   + +  +G+V  ++Y  +YD+G  +VGF
Sbjct: 325 GVEFPLPPSSYILSNNGYCTVGVEPTYLSSQNGQPLWILGDVFLRSYYSVYDLGNNRVGF 384

Query: 236 RRGA 225
              A
Sbjct: 385 ATAA 388



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>ARD3_ORYSA (Q7XNS7) Putative 1,2-dihydroxy-3-keto-5-methylthiopentene|
           dioxygenase 3 (EC 1.13.-.-) (Aci-reductone dioxygenase
           3)
          Length = 206

 Score = 27.3 bits (59), Expect = 9.0
 Identities = 14/38 (36%), Positives = 18/38 (47%)
 Frame = +1

Query: 136 LDHNELACIPQIHTIGPSHDWLSCNLPHQHAPRRNPTL 249
           LD N      ++ + GP  DW + N PH H P R   L
Sbjct: 150 LDTNNYIKTMRLFSGGP--DWTAYNRPHDHLPERKKYL 185



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>CRDL1_RAT (Q76LD0) Chordin-like protein 1 precursor (Kohjirin)|
          Length = 447

 Score = 27.3 bits (59), Expect = 9.0
 Identities = 15/41 (36%), Positives = 19/41 (46%)
 Frame = +1

Query: 163 PQIHTIGPSHDWLSCNLPHQHAPRRNPTLPPPTSYRTS*VC 285
           P +H + P H      +PH   PR   +LPP  S  TS  C
Sbjct: 76  PTLHCLSPVH------IPHLCCPRCPDSLPPMNSKVTSKSC 110



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>CYB_TRYBB (P00164) Cytochrome b|
          Length = 363

 Score = 27.3 bits (59), Expect = 9.0
 Identities = 11/12 (91%), Positives = 12/12 (100%)
 Frame = +3

Query: 87  KLHVLHLLLPFI 122
           KLHVLH+LLPFI
Sbjct: 170 KLHVLHVLLPFI 181


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,181,879
Number of Sequences: 219361
Number of extensions: 1367868
Number of successful extensions: 3940
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 3803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3940
length of database: 80,573,946
effective HSP length: 106
effective length of database: 57,321,680
effective search space used: 1375720320
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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