ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart21f06
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1E134_MAIZE (Q9ZT66) Endo-1,3;1,4-beta-D-glucanase precursor (EC ... 39 0.005
2YAE9_YEAST (P39721) Protein YAL049C 37 0.035
3DLHH_YERPE (Q8ZAL4) Putative carboxymethylenebutenolidase (EC 3.... 30 3.3
4EFTS_DESVH (Q72DQ6) Elongation factor Ts (EF-Ts) 30 4.3
5MLR_LUMTE (P80164) Myosin regulatory light chain, striated muscl... 29 5.6

>E134_MAIZE (Q9ZT66) Endo-1,3;1,4-beta-D-glucanase precursor (EC 3.2.1.-)|
          Length = 303

 Score = 39.3 bits (90), Expect = 0.005
 Identities = 18/33 (54%), Positives = 25/33 (75%)
 Frame = -2

Query: 475 EDIKEVKCPIAVLGAEIDKTSPPELVKQFEQVL 377
           +D+KEVK PI +LGA+ D T+PP+ V +F  VL
Sbjct: 181 DDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVL 213



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>YAE9_YEAST (P39721) Protein YAL049C|
          Length = 246

 Score = 36.6 bits (83), Expect = 0.035
 Identities = 25/83 (30%), Positives = 39/83 (46%), Gaps = 2/83 (2%)
 Frame = -2

Query: 478 VEDIKEV--KCPIAVLGAEIDKTSPPELVKQFEQVLSSNSGITHFVKIFPGVSHGWTVRY 305
           +E+I+ +  K PI +  AE D   P  L    E+ L  N   T+ + +F GV+HG+  R 
Sbjct: 160 IEEIEAIDSKKPILISAAEEDHIFPANLRHLTEEKLKDNHA-TYQLDLFSGVAHGFAARG 218

Query: 304 KSEDAGAVKSAEEALSHMIDWFS 236
                    + E+ L   I WF+
Sbjct: 219 DISIPAVKYAKEKVLLDQIYWFN 241



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>DLHH_YERPE (Q8ZAL4) Putative carboxymethylenebutenolidase (EC 3.1.1.45)|
           (Dienelactone hydrolase) (DLH)
          Length = 267

 Score = 30.0 bits (66), Expect = 3.3
 Identities = 16/74 (21%), Positives = 35/74 (47%)
 Frame = -2

Query: 451 PIAVLGAEIDKTSPPELVKQFEQVLSSNSGITHFVKIFPGVSHGWTVRYKSEDAGAVKSA 272
           P+  L    D + P E ++   Q L + +     + ++P   H +   Y+   +   +SA
Sbjct: 185 PVLGLYGGKDTSIPAEHIETMRQALRAANADAEII-VYPEAGHAFNADYRP--SYHAESA 241

Query: 271 EEALSHMIDWFSKN 230
           ++    M+DWF+++
Sbjct: 242 QDGWQRMLDWFTQH 255



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>EFTS_DESVH (Q72DQ6) Elongation factor Ts (EF-Ts)|
          Length = 287

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 18/52 (34%), Positives = 26/52 (50%)
 Frame = -2

Query: 424 DKTSPPELVKQFEQVLSSNSGITHFVKIFPGVSHGWTVRYKSEDAGAVKSAE 269
           DKT+  EL+KQ  + +  N G+  FV+   G           EDA A ++AE
Sbjct: 247 DKTTVAELLKQTSKAVGDNLGVARFVRFQLG-----------EDAAAEEAAE 287



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>MLR_LUMTE (P80164) Myosin regulatory light chain, striated muscle, 25 kDa|
           isoform (LC25)
          Length = 195

 Score = 29.3 bits (64), Expect = 5.6
 Identities = 19/71 (26%), Positives = 35/71 (49%)
 Frame = -2

Query: 439 LGAEIDKTSPPELVKQFEQVLSSNSGITHFVKIFPGVSHGWTVRYKSEDAGAVKSAEEAL 260
           +G E+D    P++VK+     +     THF+ +F    HG        DA A+   E+ L
Sbjct: 87  IGREVD----PKVVKEMLAESAEKLNFTHFLTLFGEKLHGTDTEGTLRDAFALFD-EDKL 141

Query: 259 SHMIDWFSKNL 227
            ++++ + K+L
Sbjct: 142 GYLLEEYVKDL 152


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 68,853,836
Number of Sequences: 219361
Number of extensions: 1347060
Number of successful extensions: 2980
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2930
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2980
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3246866728
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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