ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart21b02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SAP_CHICK (O13035) Proactivator polypeptide precursor [Contains:... 33 0.42
2OXAA_DEIRA (Q9RSH5) Membrane protein oxaA 32 0.72
3YGEZ_ECOLI (Q46806) Hypothetical hydrolase ygeZ (EC 3.-.-.-) 32 0.94
4SAP_RAT (P10960) Sulfated glycoprotein 1 precursor (SGP-1) (Pros... 31 2.1
5SAP_BOVIN (P26779) Proactivator polypeptide precursor [Contains:... 31 2.1
6SAP_HUMAN (P07602) Proactivator polypeptide precursor [Contains:... 30 2.7
7SAP_MOUSE (Q61207) Sulfated glycoprotein 1 precursor (SGP-1) (Pr... 30 4.7
8SYP32_ARATH (Q9LK09) Syntaxin-32 (AtSYP32) 30 4.7
9SEN54_SCHPO (O74908) Probable tRNA-splicing endonuclease subunit... 30 4.7
10FDHD_BACLD (Q65DY4) Protein fdhD homolog 29 6.1
11DSG3_MOUSE (O35902) Desmoglein-3 precursor (130 kDa pemphigus vu... 29 6.1
12ATG26_CRYNE (Q5KK25) Sterol 3-beta-glucosyltransferase (EC 2.4.1... 29 7.9

>SAP_CHICK (O13035) Proactivator polypeptide precursor [Contains: Saposin A;|
           Saposin B; Saposin C; Saposin D]
          Length = 518

 Score = 33.1 bits (74), Expect = 0.42
 Identities = 18/57 (31%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
 Frame = -1

Query: 498 ILLKECKKI-EGHEQQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLG 331
           +L K C  + E    QC + V QY P+++    + ++   +CT +  C A KK +LG
Sbjct: 429 LLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLGVCGAAKKPLLG 485



 Score = 29.3 bits (64), Expect = 6.1
 Identities = 19/81 (23%), Positives = 42/81 (51%), Gaps = 3/81 (3%)
 Frame = -1

Query: 495 LLKECKKI--EGHEQQCKRLVLQYIPLIL-VNGEKFLEKNDICTIVQACDAGKKTVLGSF 325
           L K C+ +  +G   +CK +V  Y+P+I+ +  E+F +   +C+ +  C + +K +    
Sbjct: 91  LEKRCEFLPDQGLASECKEIVDSYLPVIMDMIKEEFDKPEVVCSALSLCQSLQKHLAAMK 150

Query: 324 SGEGLLRDE*RQKDYTEPRKP 262
             + L  ++  + D++E   P
Sbjct: 151 LQKQLQSNKIPELDFSELTSP 171



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>OXAA_DEIRA (Q9RSH5) Membrane protein oxaA|
          Length = 428

 Score = 32.3 bits (72), Expect = 0.72
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = +1

Query: 202 ASRILDTYVQQEELVHYVSSGFTRLGVVFLPSFIAQQTL 318
           A+  L+ Y  + EL+H   SG+T L  +F P+F  Q +L
Sbjct: 159 ATSSLEVYGGKNELIHLYQSGYTELPGLFQPNFFGQISL 197



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>YGEZ_ECOLI (Q46806) Hypothetical hydrolase ygeZ (EC 3.-.-.-)|
          Length = 461

 Score = 32.0 bits (71), Expect = 0.94
 Identities = 25/79 (31%), Positives = 32/79 (40%)
 Frame = +3

Query: 15  PIISRQTETRFSIYSLFRYHYKLNTDQVISTTRLQVDIRNCSSYRSLYNTKHPENAAAAR 194
           P+I  +  + F +Y    Y YKLN D+V+   R   +            T HPEN AA  
Sbjct: 140 PMIVEEGLSSFKLY--LTYQYKLNDDEVLQALRRLHE-------SGALTTVHPENDAAIA 190

Query: 195 SKSQSNFGHLCTTRRTSAL 251
           SK         T  R  AL
Sbjct: 191 SKRAEFIAAGLTAPRYHAL 209



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>SAP_RAT (P10960) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)|
          Length = 554

 Score = 30.8 bits (68), Expect = 2.1
 Identities = 12/46 (26%), Positives = 27/46 (58%)
 Frame = -1

Query: 465 HEQQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLGS 328
           +++QC   V +Y PL+L    + ++ + +C+ +  C +  K +LG+
Sbjct: 477 YQKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVCPSAYKLLLGT 522



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>SAP_BOVIN (P26779) Proactivator polypeptide precursor [Contains: Saposin A|
           (Protein A); Saposin B (Sphingolipid activator protein
           1) (SAP-1) (Cerebroside sulfate activator) (CSAct)
           (Dispersin) (Sulfatide/GM1 activator); Saposin C
           (Co-beta-glucosidase) (A
          Length = 525

 Score = 30.8 bits (68), Expect = 2.1
 Identities = 12/46 (26%), Positives = 27/46 (58%)
 Frame = -1

Query: 465 HEQQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLGS 328
           + +QC + V +Y P+++    + ++ + +C  + AC A  K +LG+
Sbjct: 448 YRKQCDQFVTEYEPVLIEILVEVMDPSFVCLKIGACPAAHKPLLGA 493



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>SAP_HUMAN (P07602) Proactivator polypeptide precursor [Contains: Saposin A|
           (Protein A); Saposin B-Val; Saposin B (Sphingolipid
           activator protein 1) (SAP-1) (Cerebroside sulfate
           activator) (CSAct) (Dispersin) (Sulfatide/GM1
           activator); Saposin C (Co-beta-
          Length = 524

 Score = 30.4 bits (67), Expect = 2.7
 Identities = 11/46 (23%), Positives = 28/46 (60%)
 Frame = -1

Query: 465 HEQQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLGS 328
           +++QC + V +Y P+++    + ++ + +C  + AC +  K +LG+
Sbjct: 447 YQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPSAHKPLLGT 492



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>SAP_MOUSE (Q61207) Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)|
          Length = 557

 Score = 29.6 bits (65), Expect = 4.7
 Identities = 12/46 (26%), Positives = 26/46 (56%)
 Frame = -1

Query: 465 HEQQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLGS 328
           +++QC   V +Y PL+L    + ++   +C+ +  C +  K +LG+
Sbjct: 480 YQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPSAYKLLLGT 525



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>SYP32_ARATH (Q9LK09) Syntaxin-32 (AtSYP32)|
          Length = 347

 Score = 29.6 bits (65), Expect = 4.7
 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
 Frame = +1

Query: 157 IIQSIQRMQQQHAAKASRILDTYVQ-QEELVHYVSSGFTRLGVVF--LPSFIAQQ 312
           ++Q  Q+ QQQ   +   + DTY+Q + E +H V S    L  +F  L + ++QQ
Sbjct: 235 LLQQSQQQQQQQQQQMVPLQDTYMQGRAEALHTVESTIHELSSIFTQLATMVSQQ 289



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>SEN54_SCHPO (O74908) Probable tRNA-splicing endonuclease subunit sen54|
           (tRNA-intron endonuclease sen54)
          Length = 384

 Score = 29.6 bits (65), Expect = 4.7
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = -3

Query: 400 VPREERYMHHRSSMRCWQKDGARVILRGGFAA 305
           +P E  Y+  R SM CW ++G  + L+  ++A
Sbjct: 120 LPEETLYLVERGSMECWSEEGLPMSLQAVYSA 151



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>FDHD_BACLD (Q65DY4) Protein fdhD homolog|
          Length = 263

 Score = 29.3 bits (64), Expect = 6.1
 Identities = 17/61 (27%), Positives = 36/61 (59%)
 Frame = -1

Query: 459 QQCKRLVLQYIPLILVNGEKFLEKNDICTIVQACDAGKKTVLGSFSGEGLLRDE*RQKDY 280
           QQ  ++  ++   ++VNGE+F+      T+V + D+ ++ V+G  + EG++R +   K +
Sbjct: 21  QQEDQMATEFPLTVMVNGEEFV------TLVCSPDSLEELVIGFLASEGVIRFKNEIKRF 74

Query: 279 T 277
           T
Sbjct: 75  T 75



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>DSG3_MOUSE (O35902) Desmoglein-3 precursor (130 kDa pemphigus vulgaris antigen|
           homolog)
          Length = 993

 Score = 29.3 bits (64), Expect = 6.1
 Identities = 11/34 (32%), Positives = 20/34 (58%)
 Frame = -1

Query: 474 IEGHEQQCKRLVLQYIPLILVNGEKFLEKNDICT 373
           IEG + + K +    +P +  NG  F+E +++CT
Sbjct: 671 IEGAQPEDKEITNICVPPVTTNGADFMESSEVCT 704



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>ATG26_CRYNE (Q5KK25) Sterol 3-beta-glucosyltransferase (EC 2.4.1.173)|
           (Autophagy-related protein 26)
          Length = 1585

 Score = 28.9 bits (63), Expect = 7.9
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = +3

Query: 312 NPPRRMTRAPSFCQHRMLERWCIYRSSR 395
           N PR  +RAP+    +M E  C +RSSR
Sbjct: 130 NEPRTSSRAPNTASSQMAESSCDFRSSR 157


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,069,684
Number of Sequences: 219361
Number of extensions: 1406393
Number of successful extensions: 3740
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 3647
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3740
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3523384522
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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