ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart20d12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SPIKE_FIPV (P10033) Spike glycoprotein precursor (Peplomer prote... 35 0.12
2NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor ... 32 0.79
3SPIKE_CVCAI (P36300) Spike glycoprotein precursor (Peplomer prot... 30 3.0
4HERC2_HUMAN (O95714) HECT domain and RCC1-like domain-containing... 30 3.9
5HERC2_MOUSE (Q4U2R1) HECT domain and RCC1-like domain-containing... 29 5.1
6SPIKE_CVCAE (Q65984) Spike glycoprotein precursor (Peplomer prot... 29 6.7
7POLN_RRVT (P13888) Nonstructural polyprotein (Polyprotein nsP123... 28 8.7
8LYTH_STAHJ (Q4L6X7) Probable cell-wall amidase lytH precursor (E... 28 8.7

>SPIKE_FIPV (P10033) Spike glycoprotein precursor (Peplomer protein) (E2)|
          Length = 1452

 Score = 34.7 bits (78), Expect = 0.12
 Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 14/68 (20%)
 Frame = +1

Query: 190 TPWHMNTACMHARIDTPTTNPST----------TYWRYNTGTHVRTHNLA----HCTGAK 327
           T W  N   +++R  T T   S           TY++ N    ++T+ L     HCTG  
Sbjct: 203 TNWFNNVTLLYSRSSTATWEYSAAYAYQGVSNFTYYKLNNTNGLKTYELCEDYEHCTGYA 262

Query: 328 ERVFSPTS 351
             VF+PTS
Sbjct: 263 TNVFAPTS 270



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>NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor (EC 3.4.23.-)|
           (Nepenthesin-I)
          Length = 437

 Score = 32.0 bits (71), Expect = 0.79
 Identities = 18/52 (34%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
 Frame = -2

Query: 461 NYMVLDSGSGALCLTV-MGSRGLSXXXXXXXXXXXFVYDVGENTLSFAPVQC 309
           NY +  S +G +CL +   S+G+S            VYD G + +SFA  QC
Sbjct: 384 NYFISPS-NGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434



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>SPIKE_CVCAI (P36300) Spike glycoprotein precursor (Peplomer protein) (E2)|
          Length = 1451

 Score = 30.0 bits (66), Expect = 3.0
 Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 14/66 (21%)
 Frame = +1

Query: 196 WHMNTACMHARIDTPTTNPST----------TYWRYNTGTHVRTHNLA----HCTGAKER 333
           W  N   +++R  T T   S           TY++ N    ++++ L     +CTG    
Sbjct: 206 WFNNVTLLYSRTSTATWQHSAAYVYQGVSNFTYYKLNKTAGLKSYELCEDYEYCTGYATN 265

Query: 334 VFSPTS 351
           VF+PTS
Sbjct: 266 VFAPTS 271



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>HERC2_HUMAN (O95714) HECT domain and RCC1-like domain-containing protein 2|
          Length = 4834

 Score = 29.6 bits (65), Expect = 3.9
 Identities = 20/71 (28%), Positives = 32/71 (45%)
 Frame = -2

Query: 272  YRQYVVDGLVVGVSIRACMHAVFICHGVRGRVDQLRACYV*LARLGRYSTCMDGSWNYRI 93
            Y  YV + + VG+ +R C     +C G  G+V       + L R G +   +   W  + 
Sbjct: 2563 YAVYVRENIQVGMMVRCCRAYEEVCEGDVGKV-------IKLDRDGLHDLNVQCDWQQKG 2615

Query: 92   GHGWMIRWIRV 60
            G  W +R+I V
Sbjct: 2616 GTYW-VRYIHV 2625



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>HERC2_MOUSE (Q4U2R1) HECT domain and RCC1-like domain-containing protein 2|
          Length = 4836

 Score = 29.3 bits (64), Expect = 5.1
 Identities = 20/71 (28%), Positives = 32/71 (45%)
 Frame = -2

Query: 272  YRQYVVDGLVVGVSIRACMHAVFICHGVRGRVDQLRACYV*LARLGRYSTCMDGSWNYRI 93
            Y  YV + + VG+ +R C     +C G  G+V       + L R G +   +   W  + 
Sbjct: 2564 YAVYVRENVQVGMMVRCCRTYEEVCEGDVGKV-------IKLDRDGLHDLNVQCDWQQKG 2616

Query: 92   GHGWMIRWIRV 60
            G  W +R+I V
Sbjct: 2617 GTYW-VRYIHV 2626



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>SPIKE_CVCAE (Q65984) Spike glycoprotein precursor (Peplomer protein) (E2)|
          Length = 1453

 Score = 28.9 bits (63), Expect = 6.7
 Identities = 19/79 (24%), Positives = 32/79 (40%), Gaps = 23/79 (29%)
 Frame = +1

Query: 196 WHMNTACMHARIDTPTTNPST----------TYWRYNTGTHVRTHNLA----HCTGAKER 333
           W  N   +++R  + T   S           TY++ N    ++++ L     +CTG    
Sbjct: 206 WFNNVTILYSRSSSATWQKSAAYVYQGVSNFTYYKLNNTNGLKSYELCEDYEYCTGYATN 265

Query: 334 VFSPT---------SYTNW 363
           VF+PT         S+ NW
Sbjct: 266 VFAPTVGGYIPHGFSFNNW 284



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>POLN_RRVT (P13888) Nonstructural polyprotein (Polyprotein nsP1234) (P1234)|
           [Contains: Nonstructural protein 3 (nsP3); RNA-directed
           RNA polymerase nsP4 (EC 2.7.7.48) (Nonstructural protein
           4) (nsP4)] (Fragment)
          Length = 1148

 Score = 28.5 bits (62), Expect = 8.7
 Identities = 22/86 (25%), Positives = 32/86 (37%), Gaps = 8/86 (9%)
 Frame = +3

Query: 9   KTGCYRYTTDHKSPLVIYTD--------PSYHPSMAYPIIP*PIHACRVATETS*LNVAS 164
           K G   Y T+    +  Y          PS   S+  P +   + AC    E +   VAS
Sbjct: 618 KDGARTYLTEQSEKIPTYVSKYPRPVYSPSVEDSLQNPEVA--VAACNAFLEANYPTVAS 675

Query: 165 TQLIDPATHSMAYEHGMHACTDRHTY 242
            Q+ D     +    G  +C DR T+
Sbjct: 676 YQITDEYDAYLDMVDGSESCLDRATF 701



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>LYTH_STAHJ (Q4L6X7) Probable cell-wall amidase lytH precursor (EC 3.5.1.-)|
          Length = 291

 Score = 28.5 bits (62), Expect = 8.7
 Identities = 16/74 (21%), Positives = 30/74 (40%), Gaps = 8/74 (10%)
 Frame = +1

Query: 229 IDTPTTNPSTTYWRYNT--------GTHVRTHNLAHCTGAKERVFSPTSYTNWMFCCWKL 384
           +D+P  N +T YW  N          T+++   +    G ++  F     TN      +L
Sbjct: 197 LDSPNANGATVYWYKNNQEALAETLNTNIQKKAMLFNRGTRQENFQVLRQTNKPAVLLEL 256

Query: 385 PMMDRPRDPITVRH 426
             +  P D + +R+
Sbjct: 257 GYISNPTDEVMIRN 270


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,240,181
Number of Sequences: 219361
Number of extensions: 1535689
Number of successful extensions: 4565
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4346
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4559
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2968155324
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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