ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart20c09
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RETO_ESCCA (P30986) Reticuline oxidase precursor (EC 1.21.3.3) (... 77 2e-14
2RETO_PAPSO (P93479) Reticuline oxidase precursor (EC 1.21.3.3) (... 75 1e-13
3MCRA_STRLA (P43485) Mitomycin radical oxidase (EC 1.5.3.-) 31 2.2
4XYLT1_MOUSE (Q811B1) Xylosyltransferase 1 (EC 2.4.2.26) (Xylosyl... 30 2.9
5TPIS_LACDL (Q93GB7) Triosephosphate isomerase (EC 5.3.1.1) (TIM)... 30 3.7
6TPIS_LACDE (O32757) Triosephosphate isomerase (EC 5.3.1.1) (TIM)... 30 3.7
7CYAA_SACKL (P23466) Adenylate cyclase (EC 4.6.1.1) (ATP pyrophos... 29 6.4
8GPD_ASHGO (Q759G5) Glycerol-3-phosphate dehydrogenase [NAD+] (EC... 29 8.3

>RETO_ESCCA (P30986) Reticuline oxidase precursor (EC 1.21.3.3)|
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase)
          Length = 538

 Score = 77.4 bits (189), Expect = 2e-14
 Identities = 39/99 (39%), Positives = 57/99 (57%), Gaps = 4/99 (4%)
 Frame = -3

Query: 505 WYDNGTAAAEKRMSWVRGLYNEMEPYVSSNPRAVYVNYRDLDLGTNELDGNVTSYHKATV 326
           W  +      + + W+  +Y  M+P+VS NPR  YVN+ DLDLG  +  GN T  + A  
Sbjct: 422 WNQSEQKKKTEFLDWLEKVYEFMKPFVSKNPRLGYVNHIDLDLGGIDW-GNKTVVNNAIE 480

Query: 325 ----WGHKYFKGNFKRLAAVKTMVDPHDFFRNEQSIPPL 221
               WG  YF  N++RL   KT++DP++ F + QSIPP+
Sbjct: 481 ISRSWGESYFLSNYERLIRAKTLIDPNNVFNHPQSIPPM 519



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>RETO_PAPSO (P93479) Reticuline oxidase precursor (EC 1.21.3.3)|
           (Berberine-bridge-forming enzyme) (BBE)
           (Tetrahydroprotoberberine synthase)
          Length = 535

 Score = 75.1 bits (183), Expect = 1e-13
 Identities = 33/98 (33%), Positives = 56/98 (57%), Gaps = 3/98 (3%)
 Frame = -3

Query: 505 WYDNGTAAAEKRMSWVRGLYNEMEPYVSSNPRAVYVNYRDLDLGTNELDGNVTSYHKATV 326
           W  +  +   +   W+   Y+ +EP+VS  PR  YVN+ DLD+G  +     ++ +   +
Sbjct: 426 WNQDEESKIGEFSEWLAKFYDYLEPFVSKEPRVGYVNHIDLDIGGIDWRNKSSTTNAVEI 485

Query: 325 ---WGHKYFKGNFKRLAAVKTMVDPHDFFRNEQSIPPL 221
              WG +YF  N++RL   KT++DP++ F + QSIPP+
Sbjct: 486 ARNWGERYFSSNYERLVKAKTLIDPNNVFNHPQSIPPM 523



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>MCRA_STRLA (P43485) Mitomycin radical oxidase (EC 1.5.3.-)|
          Length = 447

 Score = 30.8 bits (68), Expect = 2.2
 Identities = 20/51 (39%), Positives = 24/51 (47%)
 Frame = -3

Query: 376 GTNELDGNVTSYHKATVWGHKYFKGNFKRLAAVKTMVDPHDFFRNEQSIPP 224
           GTN     V     A+V    Y   +F RL AVK   DP + FR   +IPP
Sbjct: 393 GTNLNFAGVEDISPASVEA-AYTPADFARLRAVKAQYDPDNMFRVNFNIPP 442



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>XYLT1_MOUSE (Q811B1) Xylosyltransferase 1 (EC 2.4.2.26) (Xylosyltransferase I)|
           (Peptide O-xylosyltransferase 1)
          Length = 953

 Score = 30.4 bits (67), Expect = 2.9
 Identities = 19/65 (29%), Positives = 32/65 (49%), Gaps = 2/65 (3%)
 Frame = -3

Query: 430 YVSSNPRAVYVNYRDLDLGTNELDGNVTSYHKATVWGHKYFKGNF-KRLAAVKTMVD-PH 257
           Y+  + R+ Y++ + L       +  VTS+  AT+WG   F   + + +  +  M D P 
Sbjct: 351 YIHVDKRSNYLHRQGLQFSRQYENVRVTSWKMATIWGGASFLSTYLQSMRDLLEMTDWPW 410

Query: 256 DFFRN 242
           DFF N
Sbjct: 411 DFFIN 415



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>TPIS_LACDL (Q93GB7) Triosephosphate isomerase (EC 5.3.1.1) (TIM)|
           (Triose-phosphate isomerase) (Lactacin B inducer
           protein) (IP)
          Length = 252

 Score = 30.0 bits (66), Expect = 3.7
 Identities = 15/39 (38%), Positives = 21/39 (53%)
 Frame = +2

Query: 170 DQMVSPSTIVSIILAAGKRRDALLIPEEIMGIHHGLDSC 286
           DQ+  PS + S+I A     DALL   E   +H G ++C
Sbjct: 29  DQLPDPSKVESVICAPAVDLDALLKAAEGSNLHVGAENC 67



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>TPIS_LACDE (O32757) Triosephosphate isomerase (EC 5.3.1.1) (TIM)|
           (Triose-phosphate isomerase)
          Length = 252

 Score = 30.0 bits (66), Expect = 3.7
 Identities = 15/39 (38%), Positives = 21/39 (53%)
 Frame = +2

Query: 170 DQMVSPSTIVSIILAAGKRRDALLIPEEIMGIHHGLDSC 286
           DQ+  PS + S+I A     DALL   E   +H G ++C
Sbjct: 29  DQLPDPSKVESVICAPAVDLDALLKAAEGSNLHVGAENC 67



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>CYAA_SACKL (P23466) Adenylate cyclase (EC 4.6.1.1) (ATP pyrophosphate-lyase)|
            (Adenylyl cyclase)
          Length = 1839

 Score = 29.3 bits (64), Expect = 6.4
 Identities = 14/46 (30%), Positives = 26/46 (56%), Gaps = 4/46 (8%)
 Frame = -3

Query: 385  LDLGTNELDGNVTSYHKATVWGH----KYFKGNFKRLAAVKTMVDP 260
            LD+G+N+L  N+++YH    W +    KY   +  +   +K+ +DP
Sbjct: 1078 LDVGSNQLKYNISNYHYDWNWRNNKDLKYLNFSGNKRFEIKSALDP 1123



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>GPD_ASHGO (Q759G5) Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8)|
          Length = 424

 Score = 28.9 bits (63), Expect = 8.3
 Identities = 18/57 (31%), Positives = 27/57 (47%)
 Frame = -1

Query: 414 QGLCMSTTGTSTWGRMSWTATSPAITRLQYGDTSISKVILRG*QLSRPWWIPMISSG 244
           +GL   T+   T  R S+T T     RLQ     +S+      +L RP+ + +I SG
Sbjct: 25  RGLFSQTSYARTHARTSYTHTMAGTDRLQQTSQILSRSTSSEIRLERPFKVTVIGSG 81


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,476,662
Number of Sequences: 219361
Number of extensions: 1752233
Number of successful extensions: 4401
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 4271
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4396
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3696665728
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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