Clone Name | rbart13d06 |
---|---|
Clone Library Name | barley_pub |
>OPR1_ARATH (Q8LAH7) 12-oxophytodienoate reductase 1 (EC 1.3.1.42)| (12-oxophytodienoate-10,11-reductase 1) (OPDA-reductase 1) (AtOPR1) (FS-AT-I) Length = 372 Score = 143 bits (361), Expect = 2e-34 Identities = 64/100 (64%), Positives = 79/100 (79%) Frame = -1 Query: 478 VEPRMAIVDGRRQIPHRLLPFRKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLA 299 +E RM + PH L+P RKAF GTFI+AGG+ R++GN+ V+ G DLVAYGR FLA Sbjct: 272 IEARMKTMGEVHACPHTLMPMRKAFKGTFISAGGFTREDGNEAVSKGRTDLVAYGRWFLA 331 Query: 298 NPDLPKRFELDAPLNKYDRNTFYTQDPIIGYTDYPFLDGS 179 NPDLPKRF++DAPLNKYDR TFYT DP++GYTDYPFL+ + Sbjct: 332 NPDLPKRFQVDAPLNKYDRPTFYTSDPVVGYTDYPFLEST 371
>OPR1_LYCES (Q9XG54) 12-oxophytodienoate reductase 1 (EC 1.3.1.42)| (12-oxophytodienoate-10,11-reductase 1) (OPDA-reductase 1) (LeOPR1) Length = 376 Score = 141 bits (356), Expect = 8e-34 Identities = 63/98 (64%), Positives = 80/98 (81%) Frame = -1 Query: 478 VEPRMAIVDGRRQIPHRLLPFRKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLA 299 VEPRM + + L+P RKA+ GTFI AGGYDR++GN+ + + ADLVAYGR+F++ Sbjct: 276 VEPRMKTAWEKIECTESLVPMRKAYKGTFIVAGGYDREDGNRALIEDRADLVAYGRLFIS 335 Query: 298 NPDLPKRFELDAPLNKYDRNTFYTQDPIIGYTDYPFLD 185 NPDLPKRFEL+APLNKY+R+TFYT DPI+GYTDYPFL+ Sbjct: 336 NPDLPKRFELNAPLNKYNRDTFYTSDPIVGYTDYPFLE 373
>OPR2_ARATH (Q8GYB8) 12-oxophytodienoate reductase 2 (EC 1.3.1.42)| (12-oxophytodienoate-10,11-reductase 2) (OPDA-reductase 2) (AtOPR2) Length = 374 Score = 141 bits (355), Expect = 1e-33 Identities = 64/100 (64%), Positives = 79/100 (79%) Frame = -1 Query: 478 VEPRMAIVDGRRQIPHRLLPFRKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLA 299 +EPRM V H L+P R+AF GTFI+AGG+ R++GN+ VA G DLVAYGR FLA Sbjct: 274 IEPRMKTVGEIAACSHTLMPMREAFKGTFISAGGFTREDGNEAVAKGRTDLVAYGRWFLA 333 Query: 298 NPDLPKRFELDAPLNKYDRNTFYTQDPIIGYTDYPFLDGS 179 NPDLPKRF+LDAPLNKY+R+TFYT DP++GYTDYP L+ + Sbjct: 334 NPDLPKRFQLDAPLNKYNRSTFYTSDPVVGYTDYPSLEST 373
>OPRL_LYCES (Q9FEX0) 12-oxophytodienoate reductase-like protein (EC 1.3.1.-)| (LeOPR2) Length = 355 Score = 135 bits (340), Expect = 5e-32 Identities = 58/92 (63%), Positives = 76/92 (82%) Frame = -1 Query: 460 IVDGRRQIPHRLLPFRKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLANPDLPK 281 +++ R + LLP RKAF GT IA+GGY + +G K + + YADL+++GR+FLANPDLPK Sbjct: 261 VMEPRETVNRSLLPIRKAFKGTLIASGGYGKSDGEKAIDENYADLISFGRMFLANPDLPK 320 Query: 280 RFELDAPLNKYDRNTFYTQDPIIGYTDYPFLD 185 RFE++APLNKY+R+TFYT DPIIGYTDYPFL+ Sbjct: 321 RFEVNAPLNKYNRSTFYTNDPIIGYTDYPFLE 352
>OPR3_LYCES (Q9FEW9) 12-oxophytodienoate reductase 3 (EC 1.3.1.42)| (12-oxophytodienoate-10,11-reductase 3) (OPDA-reductase 3) (LeOPR3) Length = 396 Score = 116 bits (291), Expect = 3e-26 Identities = 51/81 (62%), Positives = 66/81 (81%) Frame = -1 Query: 415 RKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFELDAPLNKYDRNT 236 R A+ GTFI +GGY R+ G + VA G ADLV+YGR+F++NPDL R +L+APLNKY+R T Sbjct: 309 RNAYQGTFICSGGYTRELGIEAVAQGDADLVSYGRLFISNPDLVMRIKLNAPLNKYNRKT 368 Query: 235 FYTQDPIIGYTDYPFLDGSNA 173 FYTQDP++GYTDYPFL G+ + Sbjct: 369 FYTQDPVVGYTDYPFLQGNGS 389
>OPR3_ARATH (Q9FUP0) 12-oxophytodienoate reductase 3 (EC 1.3.1.42)| (12-oxophytodienoate-10,11-reductase 3) (OPDA-reductase 3) (Delayed dehiscence 1) (AtOPR3) Length = 391 Score = 111 bits (278), Expect = 8e-25 Identities = 47/76 (61%), Positives = 64/76 (84%) Frame = -1 Query: 415 RKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFELDAPLNKYDRNT 236 R A+NGTF+++GG++++ G + V G ADLV+YGR+F+ANPDL RF++D LNKY+R T Sbjct: 310 RMAYNGTFMSSGGFNKELGMQAVQQGDADLVSYGRLFIANPDLVSRFKIDGELNKYNRKT 369 Query: 235 FYTQDPIIGYTDYPFL 188 FYTQDP++GYTDYPFL Sbjct: 370 FYTQDPVVGYTDYPFL 385
>OPRL1_ARATH (Q8GYA3) Putative 12-oxophytodienoate reductase-like protein 1 (EC| 1.3.1.-) Length = 324 Score = 106 bits (264), Expect = 4e-23 Identities = 51/76 (67%), Positives = 58/76 (76%) Frame = -1 Query: 478 VEPRMAIVDGRRQIPHRLLPFRKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLA 299 +EPRM V + L P R AFNGTFI AGGY R++GNK VA+G DLVAYGR+FLA Sbjct: 249 IEPRMKTVSEIFECRESLTPMRNAFNGTFIVAGGYTREDGNKAVAEGRTDLVAYGRLFLA 308 Query: 298 NPDLPKRFELDAPLNK 251 NPDLPKRFEL+APLNK Sbjct: 309 NPDLPKRFELNAPLNK 324
>OYEB_SCHPO (Q09671) Putative NADPH dehydrogenase C5H10.10 (EC 1.6.99.1) (Old| yellow enzyme homolog) Length = 392 Score = 74.3 bits (181), Expect = 1e-13 Identities = 38/78 (48%), Positives = 51/78 (65%) Frame = -1 Query: 424 LPFRKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFELDAPLNKYD 245 L ++ + FI AGG+DR ++ A+ LVA+GR FL+NPDLP R + + PLNK+D Sbjct: 305 LIYKNLWGDPFITAGGHDRDSAIQM-AEQENTLVAFGRYFLSNPDLPFRLKYNLPLNKWD 363 Query: 244 RNTFYTQDPIIGYTDYPF 191 R TFYT+ GY DYPF Sbjct: 364 RATFYTKMSPKGYIDYPF 381
>OYEA_SCHPO (Q09670) Putative NADPH dehydrogenase C5H10.04 (EC 1.6.99.1) (Old| yellow enzyme homolog) Length = 382 Score = 69.7 bits (169), Expect = 4e-12 Identities = 37/75 (49%), Positives = 48/75 (64%) Frame = -1 Query: 415 RKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFELDAPLNKYDRNT 236 +K + G FI AGGYD + + A+ LVA+GR F+ANPDL R + PLNK+DR++ Sbjct: 301 QKYWKGPFITAGGYDPETAVQA-ANERGVLVAFGRNFIANPDLVFRIKHHIPLNKWDRSS 359 Query: 235 FYTQDPIIGYTDYPF 191 FY GYTDYPF Sbjct: 360 FYLPKTEKGYTDYPF 374
>OYE3_YEAST (P41816) NADPH dehydrogenase 3 (EC 1.6.99.1) (Old yellow enzyme 3)| Length = 399 Score = 64.3 bits (155), Expect = 2e-10 Identities = 43/102 (42%), Positives = 56/102 (54%), Gaps = 7/102 (6%) Frame = -1 Query: 478 VEPRM---AIVDGRRQIPHRLLPFRKA-FNGTFIAAGGYDRKEGNKVVADGYAD---LVA 320 VEPR+ ++V+G + F + + G I AG Y +VV + D L+ Sbjct: 288 VEPRVTDPSLVEGEGEYSEGTNDFAYSIWKGPIIRAGNYALHP--EVVREQVKDPRTLIG 345 Query: 319 YGRIFLANPDLPKRFELDAPLNKYDRNTFYTQDPIIGYTDYP 194 YGR F++NPDL R E PLNKYDR+TFYT GYTDYP Sbjct: 346 YGRFFISNPDLVYRLEEGLPLNKYDRSTFYTMS-AEGYTDYP 386
>NEMA_ECOLI (P77258) N-ethylmaleimide reductase (EC 1.-.-.-) (N-ethylmaleimide| reducing enzyme) Length = 365 Score = 57.0 bits (136), Expect = 2e-08 Identities = 31/76 (40%), Positives = 41/76 (53%) Frame = -1 Query: 415 RKAFNGTFIAAGGYDRKEGNKVVADGYADLVAYGRIFLANPDLPKRFELDAPLNKYDRNT 236 R F+G I AG Y ++ ++ G D VA+GR ++ANPDL R + A LN + Sbjct: 291 RARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAES 350 Query: 235 FYTQDPIIGYTDYPFL 188 FY GYTDYP L Sbjct: 351 FY-GGGAEGYTDYPTL 365
>OYE1_SACPS (Q02899) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yellow enzyme 1)| Length = 399 Score = 57.0 bits (136), Expect = 2e-08 Identities = 27/45 (60%), Positives = 31/45 (68%) Frame = -1 Query: 328 LVAYGRIFLANPDLPKRFELDAPLNKYDRNTFYTQDPIIGYTDYP 194 L+ YGR F++NPDL R E PLNKYDR+TFY Q GY DYP Sbjct: 343 LIGYGRFFISNPDLVDRLEKGLPLNKYDRDTFY-QMSAHGYIDYP 386
>OYE2_YEAST (Q03558) NADPH dehydrogenase 2 (EC 1.6.99.1) (Old yellow enzyme 2)| Length = 399 Score = 56.6 bits (135), Expect = 3e-08 Identities = 33/74 (44%), Positives = 41/74 (55%), Gaps = 3/74 (4%) Frame = -1 Query: 406 FNGTFIAAGGYDRKEGNKVVADGYAD---LVAYGRIFLANPDLPKRFELDAPLNKYDRNT 236 + G I AG + +VV + D L+ YGR F++NPDL R E PLNKYDR+T Sbjct: 316 WKGPIIRAGNFALHP--EVVREEVKDPRTLIGYGRFFISNPDLVDRLEKGLPLNKYDRDT 373 Query: 235 FYTQDPIIGYTDYP 194 FY GY DYP Sbjct: 374 FYKMS-AEGYIDYP 386
>KYE1_KLULA (P40952) NADPH dehydrogenase 1 (EC 1.6.99.1) (Old yellow enzyme 1)| Length = 398 Score = 54.3 bits (129), Expect = 2e-07 Identities = 37/100 (37%), Positives = 46/100 (46%), Gaps = 19/100 (19%) Frame = -1 Query: 436 PHRLLPFRKAFNGTFIAAGG-----YDRKEGNKVVADGYA--------------DLVAYG 314 P PF+ F G + GG Y +GN + YA L+ YG Sbjct: 290 PRVTSPFQPEFEGWY--KGGTNEFVYSVWKGNVLRVGNYALDPDAAITDSKNPNTLIGYG 347 Query: 313 RIFLANPDLPKRFELDAPLNKYDRNTFYTQDPIIGYTDYP 194 R F+ANPDL +R E PLN+YDR +FY GY DYP Sbjct: 348 RAFIANPDLVERLEKGLPLNQYDRPSFYKMS-AEGYIDYP 386
>EBP1_CANAL (P43084) Probable NADPH dehydrogenase (EC 1.6.99.1)| (Estrogen-binding protein) (EBP) Length = 406 Score = 46.2 bits (108), Expect = 4e-05 Identities = 25/65 (38%), Positives = 36/65 (55%), Gaps = 4/65 (6%) Frame = -1 Query: 412 KAFNGTFIAAGGY--DRKEGNKVVADGYAD--LVAYGRIFLANPDLPKRFELDAPLNKYD 245 K + G FI AG Y D E ++ D D ++ + R F +NPDL ++ +L PLN Y+ Sbjct: 319 KIWKGNFIRAGNYTYDAPEFKTLINDLKNDRSIIGFSRFFTSNPDLVEKLKLGKPLNYYN 378 Query: 244 RNTFY 230 R FY Sbjct: 379 REEFY 383
>NADO_THEBR (P32382) NADH oxidase (EC 1.-.-.-)| Length = 651 Score = 33.5 bits (75), Expect = 0.29 Identities = 18/47 (38%), Positives = 26/47 (55%), Gaps = 1/47 (2%) Frame = -1 Query: 415 RKAFNGTFIAAGGYDRKE-GNKVVADGYADLVAYGRIFLANPDLPKR 278 +K N I G E K++A+G AD VA GR +A+P+ PK+ Sbjct: 286 KKVVNIPVITVGVIREPEFAEKIIAEGRADFVAVGRGLIADPEWPKK 332
>MRAW_CHLCV (Q823N6) S-adenosyl-methyltransferase mraW (EC 2.1.1.-)| Length = 297 Score = 30.8 bits (68), Expect = 1.9 Identities = 17/65 (26%), Positives = 28/65 (43%), Gaps = 4/65 (6%) Frame = +2 Query: 293 WISKKDPSVSNKISIAVGHHFVAFLSIIPS----SGNERAVECFPEGQEPVGYLSAAIHN 460 W S ++P +++ G H AFLS PS G++R +E + +H Sbjct: 18 WFSDRNPRSFCDVTVGAGGHAEAFLSTYPSIISYDGSDRDATALSLARERLEKFGDRVHL 77 Query: 461 GHSGF 475 H+ F Sbjct: 78 HHASF 82
>SYC_SYNEL (Q8DHW6) Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Cysteine--tRNA| ligase) (CysRS) Length = 486 Score = 30.4 bits (67), Expect = 2.5 Identities = 22/69 (31%), Positives = 34/69 (49%), Gaps = 2/69 (2%) Frame = +2 Query: 218 RILCVKGVAVVLVQRSVEL--KSLRQIWISKKDPSVSNKISIAVGHHFVAFLSIIPSSGN 391 R L G V VQ ++ K LR+ + + + + + IA H +A L+I+P+S Sbjct: 53 RYLTFLGYTVHYVQNFTDIDDKILRRAYENGETMATVSDRYIAAYHQDMAALNILPASAY 112 Query: 392 ERAVECFPE 418 RA E PE Sbjct: 113 PRATEVIPE 121
>DCNL2_MOUSE (Q8BZJ7) DCN1-like protein 2 (Defective in cullin neddylation| protein 2-like protein 2) (DCUN1 domain-containing protein 2) Length = 258 Score = 30.0 bits (66), Expect = 3.2 Identities = 17/44 (38%), Positives = 24/44 (54%) Frame = +2 Query: 254 VQRSVELKSLRQIWISKKDPSVSNKISIAVGHHFVAFLSIIPSS 385 ++ SV+ K L Q++ KDP NKI I F LS+ P+S Sbjct: 56 MKSSVDQKKLEQLYSRYKDPQDENKIGIDGIQQFCDDLSLDPAS 99
>Y435_BUCAI (P57510) Hypothetical protein BU435| Length = 319 Score = 30.0 bits (66), Expect = 3.2 Identities = 13/37 (35%), Positives = 23/37 (62%) Frame = +3 Query: 45 YISKLFVEMIHYTCPCIYIPEKKLLQLYKPSKSLTIV 155 ++S LFV++ + +CP I+ K +L KPS+ +V Sbjct: 128 FLSSLFVKIPNQSCPVIHEDNKTILWYEKPSERFLLV 164
>MAD1_YEAST (P40957) Spindle assembly checkpoint component MAD1 (Mitotic arrest| deficient protein 1) Length = 749 Score = 30.0 bits (66), Expect = 3.2 Identities = 13/43 (30%), Positives = 27/43 (62%) Frame = +2 Query: 176 VRAIKERIVCVANDRILCVKGVAVVLVQRSVELKSLRQIWISK 304 ++++KER ND+ C++ + L + +E+++LRQ + SK Sbjct: 124 LKSLKERSANSMNDKDKCIEELRTTLQNKDLEMETLRQQYDSK 166
>Y31K_SSV1 (P20203) Hypothetical 31.5 kDa protein (ORF B-277)| Length = 277 Score = 29.3 bits (64), Expect = 5.5 Identities = 11/39 (28%), Positives = 22/39 (56%) Frame = +2 Query: 266 VELKSLRQIWISKKDPSVSNKISIAVGHHFVAFLSIIPS 382 +E+K LR+ W+ +P + ++ G FVA ++P+ Sbjct: 129 LEVKKLRKAWVLTAEPILLERLKFPFGKKFVAVHFVLPN 167
>ISPH_CLOTE (Q895G2) 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC| 1.17.1.2) Length = 635 Score = 29.3 bits (64), Expect = 5.5 Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 3/50 (6%) Frame = +3 Query: 30 GITWEYISKLF---VEMIHYTCPCIYIPEKKLLQLYKPSKSLTIVEQMNY 170 G+T E I KL + +I TCP + +KK + YK S+ IV N+ Sbjct: 78 GVTLETIQKLKEKKLNIIDATCPYVSNIQKKAQKYYKEGYSILIVGDKNH 127
>XIST_MOUSE (P27571) X inactive-specific transcript protein (Fragment)| Length = 298 Score = 28.9 bits (63), Expect = 7.2 Identities = 22/91 (24%), Positives = 41/91 (45%), Gaps = 13/91 (14%) Frame = -2 Query: 234 FTHRILSLATQTILSLMARTLNSSSAQL*LMICLVYIIVI----IFSPV---------CI 94 F +LS + +SL + S+ + L + +C +Y++ I F+P C+ Sbjct: 108 FLLHVLSCRALSFVSLSLVPVWSALSSLAMALCFIYLVAISLSFSFTPAFRDSLWVTCCM 167 Query: 93 CTGMCSVSFPQTIYLCTPM*CQYVKIFFKLI 1 + V P + LC+ CQ + +FF L+ Sbjct: 168 HPSLFLVLTP--LVLCSSSLCQSISVFFLLL 196
>DHTM_METME (P16099) Trimethylamine dehydrogenase (EC 1.5.8.2) (TMADh)| Length = 729 Score = 28.9 bits (63), Expect = 7.2 Identities = 14/41 (34%), Positives = 25/41 (60%), Gaps = 1/41 (2%) Frame = -1 Query: 391 IAAGGY-DRKEGNKVVADGYADLVAYGRIFLANPDLPKRFE 272 + G Y D ++ ++V GYAD++ R +A+P LP++ E Sbjct: 295 LGVGRYTDPEKMIEIVTKGYADIIGCARPSIADPFLPQKVE 335
>DCNL1_MOUSE (Q9QZ73) DCN1-like protein 1 (Defective in cullin neddylation| protein 1-like protein 1) (DCUN1 domain-containing protein 1) (Testis-specific protein 3) Length = 259 Score = 28.9 bits (63), Expect = 7.2 Identities = 16/44 (36%), Positives = 24/44 (54%) Frame = +2 Query: 254 VQRSVELKSLRQIWISKKDPSVSNKISIAVGHHFVAFLSIIPSS 385 V+ S++ K L Q++ KDP NKI I F L++ P+S Sbjct: 56 VKGSLDRKKLEQLYTRYKDPQDENKIGIDGIQQFCDDLALDPAS 99
>SCK2_SCHPO (Q10364) Serine/threonine-protein kinase sck2 (EC 2.7.11.1)| Length = 646 Score = 28.5 bits (62), Expect = 9.4 Identities = 18/49 (36%), Positives = 23/49 (46%), Gaps = 1/49 (2%) Frame = +2 Query: 311 PSVSNKISIAVGHHFVAFLSIIPSSGNERAVECFPE-GQEPVGYLSAAI 454 PSVSN ++ H F S + GN + + PE EP GYL I Sbjct: 49 PSVSNLGPSSIDHPMEEFASDQSTVGNRNSNDILPEVDHEPSGYLKLQI 97
>IPYR_LEIXX (Q6ACP7) Inorganic pyrophosphatase (EC 3.6.1.1) (Pyrophosphate| phospho-hydrolase) (PPase) Length = 163 Score = 28.5 bits (62), Expect = 9.4 Identities = 15/46 (32%), Positives = 24/46 (52%), Gaps = 1/46 (2%) Frame = -1 Query: 448 RRQIPHRLLPFRKAFNGTFIAAGGY-DRKEGNKVVADGYADLVAYG 314 R++I H ++ G F+ G+ D E K+V G+A+L A G Sbjct: 117 RKEIEHFFARYKDLEPGKFVTIEGWGDAAEAEKIVQAGFANLEAQG 162 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 74,580,222 Number of Sequences: 219361 Number of extensions: 1557382 Number of successful extensions: 4205 Number of sequences better than 10.0: 28 Number of HSP's better than 10.0 without gapping: 4113 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4202 length of database: 80,573,946 effective HSP length: 103 effective length of database: 57,979,763 effective search space used: 3188886965 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)