ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart12d12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1ULE1A_HUMAN (Q9UBE0) Ubiquitin-like 1-activating enzyme E1A (SUM... 57 3e-08
2ULE1A_MOUSE (Q9R1T2) Ubiquitin-like 1-activating enzyme E1A (SUM... 57 3e-08
3UBE12_WHEAT (P31251) Ubiquitin-activating enzyme E1 2 45 1e-04
4UBE11_WHEAT (P20973) Ubiquitin-activating enzyme E1 1 45 1e-04
5UBE13_WHEAT (P31252) Ubiquitin-activating enzyme E1 3 44 3e-04
6UBA1_YEAST (P22515) Ubiquitin-activating enzyme E1 1 39 0.010
7UBE1_MOUSE (Q02053) Ubiquitin-activating enzyme E1 1 35 0.11
8UBA1_SCHPO (O94609) Ubiquitin-activating enzyme E1 1 (Poly(A)+ R... 34 0.19
9RAD31_SCHPO (P79064) DNA damage tolerance protein rad31 34 0.25
10UBE1_HUMAN (P22314) Ubiquitin-activating enzyme E1 (A1S9 protein) 34 0.25
11UBE1_RABIT (Q29504) Ubiquitin-activating enzyme E1 33 0.56
12AGAL_COFAR (Q42656) Alpha-galactosidase precursor (EC 3.2.1.22) ... 32 0.95
13Y2800_ANASP (Q8YTC2) Hypothetical WD-repeat protein alr2800 32 1.2
14FOLD_RICCN (Q92HY4) FolD bifunctional protein [Includes: Methyle... 31 2.1
15AGAL_CYATE (P14749) Alpha-galactosidase precursor (EC 3.2.1.22) ... 30 2.8
16ULA1_ARATH (P42744) NEDD8-activating enzyme E1 regulatory subuni... 30 3.6
17ULA1_CAEEL (Q18217) NEDD8-activating enzyme E1 regulatory subuni... 30 4.7
18THTPA_RAT (Q8CGV7) Thiamine-triphosphatase (EC 3.6.1.28) (ThTPase) 29 6.2
19CSF3_FELCA (O02708) Granulocyte colony-stimulating factor precur... 29 8.1
20SILP_SALTY (Q9ZHC7) Putative cation-transporting P-type ATPase (... 29 8.1

>ULE1A_HUMAN (Q9UBE0) Ubiquitin-like 1-activating enzyme E1A (SUMO-1-activating|
           enzyme subunit 1)
          Length = 346

 Score = 57.0 bits (136), Expect = 3e-08
 Identities = 29/90 (32%), Positives = 49/90 (54%)
 Frame = -3

Query: 492 ETALSDLPAVLAWRKDMCDRMSLSESQIPTALLERLLAAGKKEHPPVCAILGGILGQEVI 313
           +T   D   +L  R D+ D + +S   +P   +    +    E  PVCA++GGIL QE++
Sbjct: 260 DTYEEDSELLLQIRNDVLDSLGISPDLLPEDFVRYCFS----EMAPVCAVVGGILAQEIV 315

Query: 312 KSISCKGDTIKSFFYFDTADGKGVMEDIPP 223
           K++S +     +FF+FD   G G++E + P
Sbjct: 316 KALSQRDPPHNNFFFFDGMKGNGIVECLGP 345



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>ULE1A_MOUSE (Q9R1T2) Ubiquitin-like 1-activating enzyme E1A (SUMO-1-activating|
           enzyme subunit 1)
          Length = 350

 Score = 57.0 bits (136), Expect = 3e-08
 Identities = 29/90 (32%), Positives = 49/90 (54%)
 Frame = -3

Query: 492 ETALSDLPAVLAWRKDMCDRMSLSESQIPTALLERLLAAGKKEHPPVCAILGGILGQEVI 313
           E+   D   +L  R D+ D + +S   +P   +    +    E  PVCA++GGIL QE++
Sbjct: 264 ESYKEDAELLLQIRNDVFDSLGISPDLLPDDFVRYCFS----EMAPVCAVVGGILAQEIV 319

Query: 312 KSISCKGDTIKSFFYFDTADGKGVMEDIPP 223
           K++S +     +FF+FD   G G++E + P
Sbjct: 320 KALSQRDPPHNNFFFFDGMKGSGIVECLGP 349



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>UBE12_WHEAT (P31251) Ubiquitin-activating enzyme E1 2|
          Length = 1051

 Score = 44.7 bits (104), Expect = 1e-04
 Identities = 24/68 (35%), Positives = 37/68 (54%)
 Frame = -3

Query: 414 QIPTALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSFFYFDTADGKGVME 235
           +I   LL    +  +    P+ A+ GGI+GQEV+K+ S K   +  FFYFD+      +E
Sbjct: 373 EIDKKLLHHFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFDS------VE 426

Query: 234 DIPPTPVE 211
            +P  P+E
Sbjct: 427 SLPVDPLE 434



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>UBE11_WHEAT (P20973) Ubiquitin-activating enzyme E1 1|
          Length = 1051

 Score = 44.7 bits (104), Expect = 1e-04
 Identities = 24/68 (35%), Positives = 37/68 (54%)
 Frame = -3

Query: 414 QIPTALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSFFYFDTADGKGVME 235
           +I   LL    +  +    P+ A+ GGI+GQEV+K+ S K   +  FFYFD+      +E
Sbjct: 373 EIDKKLLHHFASGSRAVLNPMAAMFGGIVGQEVVKACSGKFHPLYQFFYFDS------VE 426

Query: 234 DIPPTPVE 211
            +P  P+E
Sbjct: 427 SLPVDPLE 434



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>UBE13_WHEAT (P31252) Ubiquitin-activating enzyme E1 3|
          Length = 1053

 Score = 43.5 bits (101), Expect = 3e-04
 Identities = 21/49 (42%), Positives = 31/49 (63%)
 Frame = -3

Query: 357 PVCAILGGILGQEVIKSISCKGDTIKSFFYFDTADGKGVMEDIPPTPVE 211
           P+ A+ GGI+GQEV+K+ S K   +  FFYFD+      +E +P  P+E
Sbjct: 395 PMAAMFGGIVGQEVVKACSGKFHPLNQFFYFDS------VESLPTYPLE 437



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>UBA1_YEAST (P22515) Ubiquitin-activating enzyme E1 1|
          Length = 1024

 Score = 38.5 bits (88), Expect = 0.010
 Identities = 18/49 (36%), Positives = 29/49 (59%)
 Frame = -3

Query: 399 LLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSFFYFDTAD 253
           L++ L    + + P V A  GG++ QEV+K+ S K   +K F YFD+ +
Sbjct: 347 LIKELSYQARGDIPGVVAFFGGLVAQEVLKACSGKFTPLKQFMYFDSLE 395



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>UBE1_MOUSE (Q02053) Ubiquitin-activating enzyme E1 1|
          Length = 1058

 Score = 35.0 bits (79), Expect = 0.11
 Identities = 17/59 (28%), Positives = 31/59 (52%)
 Frame = -3

Query: 429 SLSESQIPTALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSFFYFDTAD 253
           S+ ++ +   L+ +L      +  P+ A +GG+  QEV+K+ S K   I  + YFD  +
Sbjct: 372 SVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALE 430



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>UBA1_SCHPO (O94609) Ubiquitin-activating enzyme E1 1 (Poly(A)+ RNA transport|
           protein 3)
          Length = 1012

 Score = 34.3 bits (77), Expect = 0.19
 Identities = 15/54 (27%), Positives = 31/54 (57%)
 Frame = -3

Query: 414 QIPTALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSFFYFDTAD 253
           ++   L++ +    + +   + A LGG + QEV+K+ + K   +K +FYFD+ +
Sbjct: 337 ELDEKLIKEISYQARGDLVAMSAFLGGAVAQEVLKATTSKFYPLKQYFYFDSLE 390



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>RAD31_SCHPO (P79064) DNA damage tolerance protein rad31|
          Length = 307

 Score = 33.9 bits (76), Expect = 0.25
 Identities = 18/68 (26%), Positives = 34/68 (50%), Gaps = 1/68 (1%)
 Frame = -3

Query: 450 KDMCDRMSLSESQIPTA-LLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSF 274
           + +C    L+E  +     L +  +    +  PV +++GG++ Q+ + SIS K   I +F
Sbjct: 233 RQVCIEQKLNEKTVLNGEFLSKFSSNISFQWTPVMSVVGGVVSQDALNSISKKQFPIDNF 292

Query: 273 FYFDTADG 250
           + FD   G
Sbjct: 293 WIFDAESG 300



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>UBE1_HUMAN (P22314) Ubiquitin-activating enzyme E1 (A1S9 protein)|
          Length = 1058

 Score = 33.9 bits (76), Expect = 0.25
 Identities = 16/59 (27%), Positives = 31/59 (52%)
 Frame = -3

Query: 429 SLSESQIPTALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSFFYFDTAD 253
           ++ ++ +   L+ +L      +  P+ A +GG+  QEV+K+ S K   I  + YFD  +
Sbjct: 372 AVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALE 430



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>UBE1_RABIT (Q29504) Ubiquitin-activating enzyme E1|
          Length = 1058

 Score = 32.7 bits (73), Expect = 0.56
 Identities = 16/59 (27%), Positives = 29/59 (49%)
 Frame = -3

Query: 429 SLSESQIPTALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSFFYFDTAD 253
           ++ +  +   L+  L      +  P+ A +GG+  QEV+K+ S K   I  + YFD  +
Sbjct: 372 AVQQDSLDEDLIRNLAFVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALE 430



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>AGAL_COFAR (Q42656) Alpha-galactosidase precursor (EC 3.2.1.22) (Melibiase)|
           (Alpha-D-galactoside galactohydrolase)
          Length = 378

 Score = 32.0 bits (71), Expect = 0.95
 Identities = 20/56 (35%), Positives = 26/56 (46%)
 Frame = -2

Query: 487 STF*STCSFGLEEGYV**NVFKRVSNSYGSTGTAFSSWKEGTSSCMCNPWWHSWSG 320
           S F S C +G E+        K V NS+ +TG    SW   TS    N  W S++G
Sbjct: 171 SIFFSLCEWGEEDPAT---WAKEVGNSWRTTGDIDDSWSSMTSRADMNDKWASYAG 223



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>Y2800_ANASP (Q8YTC2) Hypothetical WD-repeat protein alr2800|
          Length = 1258

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 28/83 (33%), Positives = 35/83 (42%), Gaps = 3/83 (3%)
 Frame = +1

Query: 28   TVWRRSRVSIQYSGANATMQSKSFLPLPPIWSCHMFACLSLMIGYNNQRLMINLS*ASRK 207
            T W RS   + +S    T+ S S      IW+ H   CL   IG+ N    I  S    K
Sbjct: 810  TGWVRS---VAFSADGQTLASGSGDRTIKIWNYHTGECLKTYIGHTNSVYSIAYS-PDSK 865

Query: 208  LLYRGWGD---VLHDTFTISCVK 267
            +L  G GD    L D  T  C+K
Sbjct: 866  ILVSGSGDRTIKLWDCQTHICIK 888



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>FOLD_RICCN (Q92HY4) FolD bifunctional protein [Includes:|
           Methylenetetrahydrofolate dehydrogenase (EC 1.5.1.5);
           Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)]
          Length = 288

 Score = 30.8 bits (68), Expect = 2.1
 Identities = 15/35 (42%), Positives = 20/35 (57%)
 Frame = +2

Query: 206 NCSTGVGGMSSMTPLPSAVSK*KKLLIVSPLQDID 310
           N    + G+    PLPS++ K K L  VSP +DID
Sbjct: 86  NLDNEISGIIVQLPLPSSIDKNKILSAVSPSKDID 120



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>AGAL_CYATE (P14749) Alpha-galactosidase precursor (EC 3.2.1.22) (Melibiase)|
           (Alpha-D-galactoside galactohydrolase)
          Length = 411

 Score = 30.4 bits (67), Expect = 2.8
 Identities = 17/54 (31%), Positives = 26/54 (48%)
 Frame = -2

Query: 481 F*STCSFGLEEGYV**NVFKRVSNSYGSTGTAFSSWKEGTSSCMCNPWWHSWSG 320
           F S C +G E+  +     K + NS+ +TG    +W   TS    N  W S++G
Sbjct: 205 FFSMCEWGWEDPQI---WAKSIGNSWRTTGDIEDNWNSMTSIADSNDKWASYAG 255



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>ULA1_ARATH (P42744) NEDD8-activating enzyme E1 regulatory subunit|
           (Auxin-resistance protein AXR1)
          Length = 540

 Score = 30.0 bits (66), Expect = 3.6
 Identities = 18/57 (31%), Positives = 28/57 (49%)
 Frame = -3

Query: 417 SQIPTALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSFFYFDTADGK 247
           S +P  L+  +   G  E   V A +GGI  QEVIK ++ +   +   + F+  D K
Sbjct: 478 SVLPDDLIHEMCRFGASEIHVVSAFVGGIASQEVIKLVTKQFVPMLGTYIFNGIDHK 534



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>ULA1_CAEEL (Q18217) NEDD8-activating enzyme E1 regulatory subunit|
           (Ubiquitin-like activation protein 1)
          Length = 541

 Score = 29.6 bits (65), Expect = 4.7
 Identities = 21/63 (33%), Positives = 31/63 (49%)
 Frame = -3

Query: 450 KDMCDRMSLSESQIPTALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIKSFF 271
           KD  D  S+S     TA+ E +   G  E   + + +GGI  QE+IK  + +   I + F
Sbjct: 469 KDEQDFTSISNKVTDTAIAE-ICRFGAAELHVISSYVGGIAAQEIIKLATNQYVPIDNTF 527

Query: 270 YFD 262
            FD
Sbjct: 528 IFD 530



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>THTPA_RAT (Q8CGV7) Thiamine-triphosphatase (EC 3.6.1.28) (ThTPase)|
          Length = 223

 Score = 29.3 bits (64), Expect = 6.2
 Identities = 19/43 (44%), Positives = 26/43 (60%)
 Frame = -3

Query: 423 SESQIPTALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCK 295
           SES I T L E LL +G++E   V A+LG +  QEV   I+ +
Sbjct: 83  SESAIVTQLFE-LLGSGEQETAGVAAVLGRLKLQEVASFITTR 124



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>CSF3_FELCA (O02708) Granulocyte colony-stimulating factor precursor (G-CSF)|
           (Fragment)
          Length = 194

 Score = 28.9 bits (63), Expect = 8.1
 Identities = 17/42 (40%), Positives = 20/42 (47%)
 Frame = -3

Query: 405 TALLERLLAAGKKEHPPVCAILGGILGQEVIKSISCKGDTIK 280
           TAL ERL AA K  HP    +LG  LG       SC    ++
Sbjct: 49  TALQERLCAAHKLCHPEELVLLGHALGIPQAPLSSCSSQALQ 90



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>SILP_SALTY (Q9ZHC7) Putative cation-transporting P-type ATPase (EC 3.6.3.-)|
          Length = 824

 Score = 28.9 bits (63), Expect = 8.1
 Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
 Frame = -3

Query: 405 TALLERLLAAGK-KEHPPVCAILGGILGQEVIKSISCKGDTIKSFFYFDTADGKGVMEDI 229
           T+LL    A  K  +HP         LG  V+K+   KG  I +  +F+   GKGV  D+
Sbjct: 535 TSLLRVTAAVDKGSQHP---------LGMAVVKAAQEKGIAIPAVTHFNAPSGKGVSGDV 585


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,810,096
Number of Sequences: 219361
Number of extensions: 1527243
Number of successful extensions: 3459
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 3373
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3458
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3581144924
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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