ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name rbart11a11
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor ... 47 3e-05
2NEP2_NEPGR (Q766C2) Aspartic proteinase nepenthesin-2 precursor ... 41 0.002
3SGS3_DROME (P02840) Salivary glue protein Sgs-3 precursor 33 0.47
4YDEP_SHIFL (Q83RF3) Protein ydeP 29 5.2
5YHU3_YEAST (P38844) Protein YHR143W precursor 29 6.8
6PTFX2_ECOLI (P32670) Multiphosphoryl transfer protein 2 (MTP 2) ... 29 6.8
7YDEP_ECOLI (P77561) Protein ydeP 28 8.9
8YDEP_ECOL6 (Q8FHF8) Protein ydeP 28 8.9
9YDEP_ECO57 (Q8XAX1) Protein ydeP 28 8.9

>NEP1_NEPGR (Q766C3) Aspartic proteinase nepenthesin-1 precursor (EC 3.4.23.-)|
           (Nepenthesin-I)
          Length = 437

 Score = 46.6 bits (109), Expect = 3e-05
 Identities = 32/85 (37%), Positives = 39/85 (45%), Gaps = 5/85 (5%)
 Frame = -2

Query: 415 DTCFDF-SGQDNVTIPTVALVFDGGKVVDLDANGIIFGS----CLAFXXXXXXXXXGIIG 251
           D CF   S   N+ IPT  + FDGG +     N  I  S    CLA           I G
Sbjct: 352 DLCFQTPSDPSNLQIPTFVMHFDGGDLELPSENYFISPSNGLICLAMGSSSQGMS--IFG 409

Query: 250 NVQQRTLEVLYDVGQSVFGFKSNAC 176
           N+QQ+ + V+YD G SV  F S  C
Sbjct: 410 NIQQQNMLVVYDTGNSVVSFASAQC 434



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>NEP2_NEPGR (Q766C2) Aspartic proteinase nepenthesin-2 precursor (EC 3.4.23.-)|
           (Nepenthesin-II)
          Length = 438

 Score = 40.8 bits (94), Expect = 0.002
 Identities = 31/103 (30%), Positives = 44/103 (42%), Gaps = 6/103 (5%)
 Frame = -2

Query: 466 FKDGMKQYPTAPARSILDTCFDF-SGQDNVTIPTVALVFDGGKVVDLDANGIIFGS---- 302
           F D +       + S L TCF   S    V +P +++ FDGG V++L    I+       
Sbjct: 335 FTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILISPAEGV 393

Query: 301 -CLAFXXXXXXXXXGIIGNVQQRTLEVLYDVGQSVFGFKSNAC 176
            CLA           I GN+QQ+  +VLYD+      F    C
Sbjct: 394 ICLAMGSSSQLGIS-IFGNIQQQETQVLYDLQNLAVSFVPTQC 435



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>SGS3_DROME (P02840) Salivary glue protein Sgs-3 precursor|
          Length = 307

 Score = 32.7 bits (73), Expect = 0.47
 Identities = 21/72 (29%), Positives = 30/72 (41%)
 Frame = +3

Query: 210 PTS*STSRVLCCTXXXXXXXXXXXXXXNARQLPNMIPLASRSTTLPPSNTKATVGMVTLS 389
           PT  ST++  C T                 QLP   P  +++TT  P+ TKAT    T +
Sbjct: 58  PTQQSTTQPPCTTSKPTTPKQTTT------QLPCTTPTTTKATTTKPTTTKATTTKATTT 111

Query: 390 WPLKSKHVSRML 425
            P  +K  +  L
Sbjct: 112 KPTTTKQTTTQL 123



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>YDEP_SHIFL (Q83RF3) Protein ydeP|
          Length = 759

 Score = 29.3 bits (64), Expect = 5.2
 Identities = 9/14 (64%), Positives = 11/14 (78%)
 Frame = +1

Query: 412 CPGCSWPAPSGTAS 453
           CPGC+WP P  +AS
Sbjct: 49  CPGCAWPDPKNSAS 62



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>YHU3_YEAST (P38844) Protein YHR143W precursor|
          Length = 325

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 13/45 (28%), Positives = 25/45 (55%)
 Frame = +3

Query: 321 LASRSTTLPPSNTKATVGMVTLSWPLKSKHVSRMLLAGAVGYCFM 455
           +A  ++TL PS++  T    T S PL+S     + ++ + G C++
Sbjct: 243 IARTTSTLVPSSSVDTTSRATTSMPLESSSTQSISVSSSDGTCYV 287



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>PTFX2_ECOLI (P32670) Multiphosphoryl transfer protein 2 (MTP 2) [Includes:|
           Phosphoenolpyruvate-protein phosphotransferase (EC
           2.7.3.9) (Phosphotransferase system enzyme I) (Enzyme
           I-Ani); Phosphocarrier protein HPr (Protein H);
           Fructose-like phosphotrans
          Length = 833

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 29/99 (29%), Positives = 38/99 (38%), Gaps = 11/99 (11%)
 Frame = +2

Query: 188 LEPEDALAYVVKHLKGPLLHXXXXXXXXXXXXXXXXXXXXXYDPVGVEVDDLAAVEHQS- 364
           L P   L     HLKG LL                         VGV++D L   + Q+ 
Sbjct: 275 LTPSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTL----VGVDIDALTPWQQQTI 330

Query: 365 --DGGYGDVVLAA--------EVEARVQDAPGRRRRVLL 451
             DG  G +V+          + EARVQDA   ++RV L
Sbjct: 331 YIDGNAGAIVVEPGEAVARYYQQEARVQDALREQQRVWL 369



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>YDEP_ECOLI (P77561) Protein ydeP|
          Length = 759

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 9/14 (64%), Positives = 11/14 (78%)
 Frame = +1

Query: 412 CPGCSWPAPSGTAS 453
           CPGC+WP P  +AS
Sbjct: 49  CPGCAWPDPKHSAS 62



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>YDEP_ECOL6 (Q8FHF8) Protein ydeP|
          Length = 759

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 9/14 (64%), Positives = 11/14 (78%)
 Frame = +1

Query: 412 CPGCSWPAPSGTAS 453
           CPGC+WP P  +AS
Sbjct: 49  CPGCAWPDPKHSAS 62



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>YDEP_ECO57 (Q8XAX1) Protein ydeP|
          Length = 759

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 9/14 (64%), Positives = 11/14 (78%)
 Frame = +1

Query: 412 CPGCSWPAPSGTAS 453
           CPGC+WP P  +AS
Sbjct: 49  CPGCAWPDPKHSAS 62


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 56,543,467
Number of Sequences: 219361
Number of extensions: 940762
Number of successful extensions: 2991
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 2936
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2988
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3014947676
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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