ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl56h03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane pro... 239 2e-63
2AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane pro... 179 5e-45
3AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane pr... 176 3e-44
4HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.... 37 0.029
5HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.... 37 0.029
6HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6... 33 0.41
7HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.... 32 0.71
8HPPA_BRAJA (Q89K83) Pyrophosphate-energized proton pump (EC 3.6.... 30 4.6
9PRDM1_HUMAN (O75626) PR domain zinc finger protein 1 (PR domain-... 29 6.0
10HPPA_RHORT (O68460) Pyrophosphate-energized proton pump (EC 3.6.... 29 7.8

>AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase)
          Length = 762

 Score =  239 bits (611), Expect = 2e-63
 Identities = 118/128 (92%), Positives = 119/128 (92%)
 Frame = +3

Query: 99  MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXKNGYGDYLIEEEE 278
           MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTP         KNGYGDYLIEEEE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAAAGAKNGYGDYLIEEEE 60

Query: 279 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 458
           GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP
Sbjct: 61  GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 120

Query: 459 CTYSRGTC 482
           CTYS+GTC
Sbjct: 121 CTYSKGTC 128



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>AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase) (Vacuolar
           H(+)-pyrophosphatase)
          Length = 764

 Score =  179 bits (453), Expect = 5e-45
 Identities = 89/131 (67%), Positives = 102/131 (77%), Gaps = 1/131 (0%)
 Frame = +3

Query: 102 AILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXX-XXXXKNGYGDYLIEEEE 278
           AIL +LGTEILIPVC VIGI FA+ QW +VSKVK++           KNGY DYLIEEEE
Sbjct: 3   AILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPNAAAKNGYNDYLIEEEE 62

Query: 279 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 458
           G+NDHNVVVKCAEIQ AISEGATSFLFT Y+YVG+FMV FA +IFLFLGS+EGFST  Q 
Sbjct: 63  GINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFSTSPQA 122

Query: 459 CTYSRGTCSQP 491
           C+Y +    +P
Sbjct: 123 CSYDKTKTCKP 133



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>AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane proton pump 1|
           (EC 3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase 1) (H(+)-PPase 1) (Vacuolar proton
           pyrophosphatase 1) (Vacuolar proton pyrophosphatase 3)
          Length = 770

 Score =  176 bits (446), Expect = 3e-44
 Identities = 85/138 (61%), Positives = 105/138 (76%), Gaps = 4/138 (2%)
 Frame = +3

Query: 90  LTAMAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXX----KNGYGD 257
           + A A+L EL TEIL+P+C VIGI F++ QW++VS+VK+T              KNGYGD
Sbjct: 1   MVAPALLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGD 60

Query: 258 YLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEG 437
           YLIEEEEG+ND +VV KCAEIQTAISEGATSFLFT Y+YVG+FM+ FAA+IF+FLGS+EG
Sbjct: 61  YLIEEEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEG 120

Query: 438 FSTKGQPCTYSRGTCSQP 491
           FST  +PCTY      +P
Sbjct: 121 FSTDNKPCTYDTTRTCKP 138



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>HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 37.0 bits (84), Expect = 0.029
 Identities = 20/48 (41%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
 Frame = +3

Query: 306 KCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGS--IEGFS 443
           K  EI +AISEGA +FL   Y+ + +F+   A +I L L +   EGF+
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN 89



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>HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 37.0 bits (84), Expect = 0.029
 Identities = 20/48 (41%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
 Frame = +3

Query: 306 KCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGS--IEGFS 443
           K  EI +AISEGA +FL   Y+ + +F+   A +I L L +   EGF+
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN 89



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>HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 725

 Score = 33.1 bits (74), Expect = 0.41
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSI 431
           EI  AI +GA ++L   Y+ + +   V A +IF FLG +
Sbjct: 40  EIAGAIQQGAAAYLARQYKTIAIVGAVLAILIFFFLGGL 78



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>HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 673

 Score = 32.3 bits (72), Expect = 0.71
 Identities = 15/37 (40%), Positives = 24/37 (64%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 425
           EI   I +GA +FL T Y+Y+  F+V+   I+ +F+G
Sbjct: 38  EIAGHIHDGAMAFLKTEYKYLTGFIVIVTVILAIFVG 74



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>HPPA_BRAJA (Q89K83) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 706

 Score = 29.6 bits (65), Expect = 4.6
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 425
           EI  A+ EGA ++L   Y  +G+  +V   ++  FLG
Sbjct: 37  EIAAAVREGAQAYLRRQYTTIGIVGIVIFVLLVYFLG 73



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>PRDM1_HUMAN (O75626) PR domain zinc finger protein 1 (PR domain-containing|
           protein 1) (Beta-interferon gene positive-regulatory
           domain I-binding factor) (BLIMP-1) (Positive regulatory
           domain I-binding factor 1) (PRDI-binding factor 1)
           (PRDI-BF1)
          Length = 789

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = -2

Query: 454 WPFVLNPSIDPRKRKIIAAKTTMNIPTYWYMVKRNDVAPSEMAVW 320
           W   +NP+  PR++ + A +  MNI  Y+Y +K    A  E+ VW
Sbjct: 122 WMRYVNPAHSPREQNLAACQNGMNI--YFYTIKPIP-ANQELLVW 163



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>HPPA_RHORT (O68460) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 702

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGF 440
           EI  A+ EGA++FL   Y+ + +   V   I+   LG   GF
Sbjct: 38  EISGAVQEGASAFLNRQYKTIAVVGAVVFVILTALLGISVGF 79


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,317,300
Number of Sequences: 219361
Number of extensions: 557228
Number of successful extensions: 1973
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1924
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1967
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3465624120
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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