ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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| Clone Name | bastl53g04 |
|---|---|
| Clone Library Name | barley_pub |
>AGOL_ARATH (Q9SJK3) Argonaute-like protein At2g27880| Length = 997 Score = 56.2 bits (134), Expect = 4e-08 Identities = 41/105 (39%), Positives = 56/105 (53%), Gaps = 5/105 (4%) Frame = +3 Query: 39 GLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKLQHTYR 218 G G G+ + + ANH+ V V + + HY V++ E V SK + R V+ L Y+ Sbjct: 152 GRGTLGKKVMVRANHFLVQVADRDLY--HYDVSINPE----VISKTVNRNVMKLLVKNYK 205 Query: 219 -SELADKDFAYDGEKSLFTIGALPQINNEFIVVL----EDIASGK 338 S L K AYDG KSL+T G LP + EF+V L D +SGK Sbjct: 206 DSHLGGKSPAYDGRKSLYTAGPLPFDSKEFVVNLAEKRADGSSGK 250
>AGO1_ARATH (O04379) Argonaute protein| Length = 1048 Score = 52.0 bits (123), Expect = 7e-07 Identities = 36/102 (35%), Positives = 55/102 (53%), Gaps = 3/102 (2%) Frame = +3 Query: 39 GLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKLQHTYR 218 G G+ G+ + ANH+ + + +HY V + E V S+G+ R V+ +L YR Sbjct: 184 GKGQSGKRCIVKANHFFAELPDKD--LHHYDVTITPE----VTSRGVNRAVMKQLVDNYR 237 Query: 219 -SELADKDFAYDGEKSLFTIGALPQINNEFIVVL--EDIASG 335 S L + AYDG KSL+T G LP + EF + L E++ +G Sbjct: 238 DSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRINLLDEEVGAG 279
>I2C1_HUMAN (Q9UL18) Eukaryotic translation initiation factor 2C 1 (eIF2C 1)| (eIF-2C 1) (Argonaute-1) (Putative RNA-binding protein Q99) Length = 857 Score = 52.0 bits (123), Expect = 7e-07 Identities = 34/101 (33%), Positives = 59/101 (58%), Gaps = 2/101 (1%) Frame = +3 Query: 6 PRRHIMARPAN-GLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIG 182 P + + P G+G G+PI+LLAN+++V + + + HY V++K D P + + Sbjct: 16 PLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVY--HYEVDIK-PDKCP---RRVN 69 Query: 183 RKVLD-KLQHTYRSELADKDFAYDGEKSLFTIGALPQINNE 302 R+V++ +QH D+ YDG+K+++T+ ALP I NE Sbjct: 70 REVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALP-IGNE 109
>I2C4_MOUSE (Q8CJF8) Eukaryotic translation initiation factor 2C 4 (eIF2C 4)| (eIF-2C 4) (Argonaute-4) (Piwi/argonaute family protein meIF2C4) Length = 861 Score = 50.4 bits (119), Expect = 2e-06 Identities = 27/84 (32%), Positives = 51/84 (60%), Gaps = 1/84 (1%) Frame = +3 Query: 39 GLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKLQHTYR 218 GLG G+PI+LLANH++V + + + HY V++K E + + R+V+D + ++ Sbjct: 20 GLGTVGKPIRLLANHFQVQIPKIDVY--HYDVDIKPEK----RPRRVNREVVDTMVRHFK 73 Query: 219 SEL-ADKDFAYDGEKSLFTIGALP 287 ++ D+ YDG+++++T LP Sbjct: 74 MQIFGDRQPGYDGKRNMYTAHPLP 97
>I2C4_HUMAN (Q9HCK5) Eukaryotic translation initiation factor 2C 4 (eIF2C 4)| (eIF-2C 4) (Argonaute-4) Length = 861 Score = 50.4 bits (119), Expect = 2e-06 Identities = 27/84 (32%), Positives = 51/84 (60%), Gaps = 1/84 (1%) Frame = +3 Query: 39 GLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKLQHTYR 218 GLG G+PI+LLANH++V + + + HY V++K E + + R+V+D + ++ Sbjct: 20 GLGTVGKPIRLLANHFQVQIPKIDVY--HYDVDIKPEK----RPRRVNREVVDTMVRHFK 73 Query: 219 SEL-ADKDFAYDGEKSLFTIGALP 287 ++ D+ YDG+++++T LP Sbjct: 74 MQIFGDRQPGYDGKRNMYTAHPLP 97
>I2C1_MOUSE (Q8CJG1) Eukaryotic translation initiation factor 2C 1 (eIF2C 1)| (eIF-2C 1) (Argonaute-1) (Piwi/argonaute family protein meIF2C1) Length = 857 Score = 50.4 bits (119), Expect = 2e-06 Identities = 33/101 (32%), Positives = 59/101 (58%), Gaps = 2/101 (1%) Frame = +3 Query: 6 PRRHIMARPAN-GLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIG 182 P + + P G+G G+PI+LLAN+++V + + + HY V++K D P + + Sbjct: 16 PLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVY--HYEVDIK-PDKCP---RRVN 69 Query: 183 RKVLD-KLQHTYRSELADKDFAYDGEKSLFTIGALPQINNE 302 R+V++ +QH D+ YDG+++++T+ ALP I NE Sbjct: 70 REVVEYMVQHFKPQIFGDRKPVYDGKENIYTVTALP-IGNE 109
>PINH_ARATH (Q9XGW1) PINHEAD protein (ZWILLE protein)| Length = 988 Score = 48.9 bits (115), Expect = 6e-06 Identities = 36/110 (32%), Positives = 52/110 (47%), Gaps = 1/110 (0%) Frame = +3 Query: 9 RRHIMARPANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRK 188 R++ P G G G + ANH+ + + N Y V + E V SK + R Sbjct: 123 RKNSNFAPRPGFGTLGTKCIVKANHFLADLPTKD--LNQYDVTITPE----VSSKSVNRA 176 Query: 189 VLDKLQHTYR-SELADKDFAYDGEKSLFTIGALPQINNEFIVVLEDIASG 335 ++ +L Y+ S+L + AYDG KSL+T G LP EF V + D G Sbjct: 177 IIAELVRLYKESDLGRRLPAYDGRKSLYTAGELPFTWKEFSVKIVDEDDG 226
>I2C3_MOUSE (Q8CJF9) Eukaryotic translation initiation factor 2C 3 (eIF2C 3)| (eIF-2C 3) (Argonaute-3) (Piwi/argonaute family protein meIF2C3) Length = 860 Score = 46.2 bits (108), Expect = 4e-05 Identities = 30/84 (35%), Positives = 49/84 (58%), Gaps = 1/84 (1%) Frame = +3 Query: 39 GLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLD-KLQHTY 215 G G G+PI+LLAN ++V + + + Y V++K D P + + R+V+D K+QH Sbjct: 22 GYGTMGKPIKLLANCFQVEIPKIDVYL--YEVDIK-PDKCP---RRVNREVVDSKVQHFK 75 Query: 216 RSELADKDFAYDGEKSLFTIGALP 287 + D+ YDG++SL+T LP Sbjct: 76 VTIFGDRRPVYDGKRSLYTANPLP 99
>I2C3_HUMAN (Q9H9G7) Eukaryotic translation initiation factor 2C 3 (eIF2C 3)| (eIF-2C 3) (Argonaute-3) Length = 860 Score = 45.1 bits (105), Expect = 8e-05 Identities = 32/96 (33%), Positives = 53/96 (55%), Gaps = 1/96 (1%) Frame = +3 Query: 3 KPRRHIMARPANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIG 182 +P + RP G G G+PI+LLAN ++V + + + Y V++K D P + + Sbjct: 12 QPLLMVPRRP--GYGTMGKPIKLLANCFQVEIPKIDVYL--YEVDIK-PDKCP---RRVN 63 Query: 183 RKVLDKL-QHTYRSELADKDFAYDGEKSLFTIGALP 287 R+V+D + QH + D+ YDG++SL+T LP Sbjct: 64 REVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLP 99
>I2C2_RABIT (O77503) Eukaryotic translation initiation factor 2C 2 (eIF2C 2)| (eIF-2C 2) (Argonaute-2) (Fragment) Length = 840 Score = 35.0 bits (79), Expect = 0.088 Identities = 20/87 (22%), Positives = 47/87 (54%), Gaps = 1/87 (1%) Frame = +3 Query: 30 PANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKLQH 209 P G G+ I+L AN +++ + + + HY +++K E + + R++++ + Sbjct: 8 PRPDFGTSGRTIKLQANFFEMDIPKIDIY--HYELDIKPEKCP----RRVNREIVEHMVQ 61 Query: 210 TYRSEL-ADKDFAYDGEKSLFTIGALP 287 +++++ D+ +DG K+L+T LP Sbjct: 62 HFKAQIFGDRKPVFDGRKNLYTAMPLP 88
>I2C2_MOUSE (Q8CJG0) Eukaryotic translation initiation factor 2C 2 (eIF2C 2)| (eIF-2C 2) (Argonaute-2) (Slicer protein) (Piwi/argonaute family protein meIF2C2) Length = 860 Score = 35.0 bits (79), Expect = 0.088 Identities = 20/87 (22%), Positives = 47/87 (54%), Gaps = 1/87 (1%) Frame = +3 Query: 30 PANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKLQH 209 P G G+ I+L AN +++ + + + HY +++K E + + R++++ + Sbjct: 28 PRPDFGTTGRTIKLQANFFEMDIPKIDIY--HYELDIKPEKRP----RRVNREIVEHMVQ 81 Query: 210 TYRSEL-ADKDFAYDGEKSLFTIGALP 287 +++++ D+ +DG K+L+T LP Sbjct: 82 HFKTQIFGDRKPVFDGRKNLYTAMPLP 108
>I2C2_BOVIN (Q6QME8) Eukaryotic translation initiation factor 2C 2 (eIF2C 2)| (eIF-2C 2) (Argonaute-2) Length = 860 Score = 35.0 bits (79), Expect = 0.088 Identities = 20/87 (22%), Positives = 47/87 (54%), Gaps = 1/87 (1%) Frame = +3 Query: 30 PANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKLQH 209 P G G+ I+L AN +++ + + + HY +++K E + + R++++ + Sbjct: 28 PRPDFGTSGRTIKLQANFFEMDIPKIDIY--HYELDIKPEKCP----RRVNREIVEHMVQ 81 Query: 210 TYRSEL-ADKDFAYDGEKSLFTIGALP 287 +++++ D+ +DG K+L+T LP Sbjct: 82 HFKTQIFGDRKPVFDGRKNLYTAMPLP 108
>I2C2_RAT (Q9QZ81) Eukaryotic translation initiation factor 2C 2 (eIF2C 2)| (eIF-2C 2) (Argonaute-2) (Golgi ER protein 95 kDa) (GERp95) Length = 860 Score = 34.7 bits (78), Expect = 0.11 Identities = 20/87 (22%), Positives = 47/87 (54%), Gaps = 1/87 (1%) Frame = +3 Query: 30 PANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKLQH 209 P G G+ I+L AN +++ + + + HY +++K E + + R++++ + Sbjct: 28 PRPDFGTTGRTIKLQANFFEMDIPKIDIY--HYELDIKPEKCP----RRVNREIVEHMVQ 81 Query: 210 TYRSEL-ADKDFAYDGEKSLFTIGALP 287 +++++ D+ +DG K+L+T LP Sbjct: 82 HFKTQIFGDRKPVFDGRKNLYTAMPLP 108
>BTN2_YEAST (P53286) Protein BTN2| Length = 410 Score = 32.7 bits (73), Expect = 0.44 Identities = 27/104 (25%), Positives = 48/104 (46%), Gaps = 14/104 (13%) Frame = +3 Query: 66 QLLANHYKVSVKPSEEFF-NHYYVNLKYEDDT-------PVDSKGIGRKVLDKLQHTYRS 221 +L NHY+ + ++FF N YYV + ++D +D + IG+K+ L Y Sbjct: 96 ELKENHYRPTYHVVQDFFGNQYYVEDEADEDALLRSALKDLDFRAIGKKIAKDLFQDYEI 155 Query: 222 EL---ADKDFAYDGEKSLFTIGALPQINNEFIVV---LEDIASG 335 EL D+ + +F +L Q+ + V+ +E+I G Sbjct: 156 ELNHRGDELSILSKKDKIFKEFSLDQVFEDVFVIGCGVENIDDG 199
>RNZ_CLOPE (Q8XKN1) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNase Z) (tRNA 3| endonuclease) Length = 316 Score = 32.7 bits (73), Expect = 0.44 Identities = 14/38 (36%), Positives = 22/38 (57%) Frame = +3 Query: 12 RHIMARPANGLGKKGQPIQLLANHYKVSVKPSEEFFNH 125 +H+ + A L KKGQ +LL H+ S+ +EF N+ Sbjct: 256 KHMTFKEAANLAKKGQVKELLLTHFSPSINDPKEFINN 293
>YO43_CAEEL (P34681) Hypothetical protein ZK757.3| Length = 1040 Score = 32.3 bits (72), Expect = 0.57 Identities = 28/110 (25%), Positives = 53/110 (48%), Gaps = 3/110 (2%) Frame = +3 Query: 12 RHIMARPANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDS---KGIG 182 R ++ARP GLG G+ I + +N + + +K + Y+V + + +D + I Sbjct: 84 REVVARP--GLGTIGRQIPVKSNFFAMDLKNPKMVVIQYHVEIHHPGCRKLDKDEMRIIF 141 Query: 183 RKVLDKLQHTYRSELADKDFAYDGEKSLFTIGALPQINNEFIVVLEDIAS 332 K + + + ++ A AYDG L+T+ L +++ V L+ AS Sbjct: 142 WKAVSDHPNIFHNKFA---LAYDGAHQLYTVARLEFPDDQGSVRLDCEAS 188
>SYI_SULTO (Q975H9) Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Isoleucine--tRNA| ligase) (IleRS) Length = 1047 Score = 30.4 bits (67), Expect = 2.2 Identities = 18/67 (26%), Positives = 35/67 (52%), Gaps = 4/67 (5%) Frame = +3 Query: 18 IMARPANGLGKKGQPIQLLANHYKVSVKPSEEF----FNHYYVNLKYEDDTPVDSKGIGR 185 I+A+ + + +G+P+Q+ NH + + E + FN V LK E + +GI R Sbjct: 910 IIAKGYHEVVIEGEPVQIKVNHVNILEETEEGYISARFNKGVVVLKKEISKEEEEEGIVR 969 Query: 186 KVLDKLQ 206 ++ ++Q Sbjct: 970 DIIRRIQ 976
>PYRG_SULTO (Q976E9) CTP synthase (EC 6.3.4.2) (UTP--ammonia ligase) (CTP| synthetase) Length = 531 Score = 30.4 bits (67), Expect = 2.2 Identities = 15/37 (40%), Positives = 24/37 (64%) Frame = +3 Query: 93 SVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKL 203 +VKP E F++Y VN+ YE ++ +G+ K+L KL Sbjct: 230 NVKP-EYIFSNYDVNMTYEVPLILEKQGLASKILSKL 265
>M9H3_BOMMX (Q58T55) Maximins 9/H3 precursor [Contains: Maximin-9; Maximin-H3]| Length = 144 Score = 29.3 bits (64), Expect = 4.8 Identities = 21/59 (35%), Positives = 34/59 (57%) Frame = +3 Query: 69 LLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKVLDKLQHTYRSELADKDFA 245 L+A+ Y SVK E+ + V E+++ + +GIGRK L ++ T+R + KDFA Sbjct: 12 LIASAYARSVKNDEQSLSQRDV---LEEESLREIRGIGRKFLGGVKTTFRCGV--KDFA 65
>BXB_CLOBO (P10844) Botulinum neurotoxin type B precursor (EC 3.4.24.69)| (BoNT/B) (Bontoxilysin B) [Contains: Botulinum neurotoxin B light chain; Botulinum neurotoxin B heavy chain] Length = 1290 Score = 29.3 bits (64), Expect = 4.8 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = +3 Query: 99 KPSEEFFNHYYVNLKYEDDTPVDSKGIGRKV 191 K + E N+ +NL+Y+D+ +D G G KV Sbjct: 854 KYNSEILNNIILNLRYKDNNLIDLSGYGAKV 884
>RL25_XYLFT (Q87A23) 50S ribosomal protein L25 (General stress protein CTC)| Length = 207 Score = 29.3 bits (64), Expect = 4.8 Identities = 22/78 (28%), Positives = 35/78 (44%), Gaps = 1/78 (1%) Frame = +3 Query: 12 RHIMARPANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKV 191 RH PA G + +P+ + NH ++ + E+F ++L VD G + + Sbjct: 23 RHAGMIPAIIYGGEQRPVSIQLNHEQIWLAQQNEWFYSSILDLN------VDGAGAEKVL 76 Query: 192 LDKLQ-HTYRSELADKDF 242 L LQ H YR + DF Sbjct: 77 LRDLQRHPYRQLVMHVDF 94
>RL25_XYLFA (Q9PA77) 50S ribosomal protein L25 (General stress protein CTC)| Length = 208 Score = 29.3 bits (64), Expect = 4.8 Identities = 24/79 (30%), Positives = 36/79 (45%), Gaps = 2/79 (2%) Frame = +3 Query: 12 RHIMARPANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDDTPVDSKGIGRKV 191 RH PA G + +P+ + NH ++ + E+F ++L VD G G KV Sbjct: 23 RHAGMIPAIIYGGEQRPVSIQLNHEQIWLAQQNEWFYSSILDLN------VDGGG-GEKV 75 Query: 192 L--DKLQHTYRSELADKDF 242 L D +H YR + DF Sbjct: 76 LLRDLQRHPYRQLVMHVDF 94
>ISPH_THEMA (Q9X1F7) 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC| 1.17.1.2) Length = 275 Score = 28.9 bits (63), Expect = 6.3 Identities = 20/54 (37%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = -1 Query: 341 SLSTSDVFQYHDEFIVDLRKSSDSEEALF-TIICKVLIRELRPVGVLQLIKDLS 183 S +TS + +Y EF+ + + ++ EEA+F IC V + R V L I DLS Sbjct: 149 SQTTSSLEEYK-EFVAAMIRMNEFEEAVFLNTICPVTVNREREVEELSKICDLS 201
>PIWL3_HUMAN (Q7Z3Z3) Piwi-like protein 3| Length = 882 Score = 28.9 bits (63), Expect = 6.3 Identities = 14/37 (37%), Positives = 22/37 (59%) Frame = +3 Query: 45 GKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYEDD 155 G +G +QLLANH++V +P + Y N+ Y+ D Sbjct: 119 GSEGTVVQLLANHFRVISRPQ---WVAYKYNVDYKPD 152
>EMS_DROME (P18488) Homeotic protein empty spiracles| Length = 497 Score = 28.9 bits (63), Expect = 6.3 Identities = 20/75 (26%), Positives = 39/75 (52%), Gaps = 7/75 (9%) Frame = +3 Query: 6 PRRHIMARPANGL------GKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLKYE-DDTPV 164 P H+MA PA+GL ++ Q L A H + P+++ H ++ ++++ TP Sbjct: 79 PPHHLMAPPAHGLPYPHPHAQQQQQQHLQAPHPHPHLSPAQQHVLHQHLLMQHQHPGTPK 138 Query: 165 DSKGIGRKVLDKLQH 209 + I +++L +L H Sbjct: 139 SHQDI-QELLQRLHH 152
>DPOM_PODAN (Q01529) Probable DNA polymerase (EC 2.7.7.7)| Length = 1197 Score = 28.5 bits (62), Expect = 8.2 Identities = 29/105 (27%), Positives = 47/105 (44%), Gaps = 11/105 (10%) Frame = +3 Query: 27 RPANGLGKKGQPIQLLANHYKVSVKPSEEFFNHYYVNLK----YEDDTPVDSKGIGRKVL 194 +P + + KK Q +HY++S ++ + +K Y T +DS I L Sbjct: 466 KPISDVNKKSQN----KDHYEISTILRDDRILKCVIKVKTPSGYNKITFIDSYNILPDKL 521 Query: 195 DKLQHTYRSELADKDFAYDGEKS--LFTIGALP-----QINNEFI 308 D L ++ +E+ F Y+ KS L +G P +INNE I Sbjct: 522 DNLAKSFGTEIQKGLFPYEFVKSNTLNYVGITPSIEYYKINNEVI 566 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 59,593,646 Number of Sequences: 219361 Number of extensions: 1094421 Number of successful extensions: 3824 Number of sequences better than 10.0: 26 Number of HSP's better than 10.0 without gapping: 3673 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3818 length of database: 80,573,946 effective HSP length: 102 effective length of database: 58,199,124 effective search space used: 2793557952 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)
