ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl44d04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1ref|XM_476056.1| Oryza sativa (japonica cultivar-group), predic... 151 2e-33
2gb|AC087553.3| Oryza sativa (japonica cultivar-group) chromosome... 151 2e-33
3gb|AC084817.5| Oryza sativa (japonica cultivar-group) chromosome... 151 2e-33
4dbj|AP008211.1| Oryza sativa (japonica cultivar-group) genomic D... 151 2e-33
5ref|NM_193792.1| Oryza sativa (japonica cultivar-group), predic... 44 0.39
6gb|AC153489.7| Mus musculus 10 BAC RP23-95A4 (Roswell Park Cance... 44 0.39
7gb|AC124360.5| Mus musculus BAC clone RP24-502M22 from chromosom... 44 0.39
8emb|AL355595.10| Human DNA sequence from clone RP11-276G3 on chr... 44 0.39
9dbj|AP008207.1| Oryza sativa (japonica cultivar-group) genomic D... 44 0.39
10dbj|AP003202.3| Oryza sativa (japonica cultivar-group) genomic D... 44 0.39
11emb|AL135818.3|CNS01DVH Human chromosome 14 DNA sequence BAC C-2... 44 0.39
12emb|AL133153.3|CNS01DU3 Human chromosome 14 DNA sequence BAC R-8... 44 0.39
13gb|AC124597.5| Mus musculus BAC clone RP23-21K1 from chromosome ... 40 6.1
14gb|AF527417.1| Homo sapiens cell division cycle 25A (CDC25A) gen... 40 6.1
15gb|AC125401.4| Mus musculus BAC clone RP24-538H16 from chromosom... 40 6.1
16gb|AC128673.4| Homo sapiens BAC clone RP11-1195P3 from UL, compl... 40 6.1
17gb|AC164882.2| Mus musculus BAC clone RP23-88F16 from chromosome... 40 6.1
18emb|AL732314.18| Human DNA sequence from clone RP13-465B17 on ch... 40 6.1
19emb|AL360270.18| Human DNA sequence from clone RP11-96K19 on chr... 40 6.1
20emb|AL353802.14| Human DNA sequence from clone RP4-662P1 on chro... 40 6.1
21emb|AL138741.13| Human DNA sequence from clone RP3-516D24 on chr... 40 6.1
22gb|BC039099.1| Homo sapiens cDNA clone IMAGE:4824737 40 6.1
23gb|AC175824.2| Pan troglodytes BAC clone CH251-171H18 from chrom... 40 6.1
24dbj|AK092143.1| Homo sapiens cDNA FLJ34824 fis, clone NT2NE2008783 40 6.1
25gb|AC109584.2| Homo sapiens chromosome 3 clone RP11-674P14, comp... 40 6.1
26gb|AC011773.9| Homo sapiens chromosome 8, clone RP11-219B4, comp... 40 6.1
27gb|AC074135.3| Homo sapiens chromosome 19 clone CTD-2291D10, com... 40 6.1
28dbj|AP007155.1| Aspergillus oryzae RIB40 genomic DNA, SC003 40 6.1
29gb|AC092797.2| Homo sapiens chromosome 3 clone RP11-452E8, compl... 40 6.1
30gb|AC092290.3| Homo sapiens chromosome 5 clone CTD-2376K3, compl... 40 6.1
31gb|AC161621.5| Pan troglodytes BAC clone CH251-164P18 from chrom... 40 6.1
32gb|AC124916.3| Homo sapiens chromosome 3 clone RP11-1029M24, com... 40 6.1
33gb|AC104190.3| Homo sapiens chromosome 3 clone RP11-502L5, compl... 40 6.1
34dbj|AP006564.1| Homo sapiens genomic DNA, chromosome 18 clone:RP... 40 6.1

>ref|XM_476056.1| Oryza sativa (japonica cultivar-group),  predicted mRNA
          Length = 3354

 Score =  151 bits (76), Expect = 2e-33
 Identities = 172/204 (84%)
 Strand = Plus / Plus

                                                                       
Query: 248 tcctctccggctggtcgccggaggctgatgtctgctcctggcacggtgtttcatgcctga 307
           ||||||||||||||||||||||||| || || ||||| |||||||| ||  | |||||||
Sbjct: 539 tcctctccggctggtcgccggaggccgacgtgtgctcatggcacggcgtgacgtgcctga 598

                                                                       
Query: 308 caggagagggcattgtgacaggcctcaacttgtcgggatacggcctatcgggcaccttat 367
           |||| ||||||||||| || ||||||||| ||||||| |||||||| || |||||  |||
Sbjct: 599 caggggagggcattgtcaccggcctcaacctgtcggggtacggcctgtccggcacgatat 658

                                                                       
Query: 368 cgccggcgatagctggcctgatatccgtggagatcattgacctctcctccaactccctca 427
           ||||||||||||| |||||  ||||||| |||  |||||| || || || || |||||||
Sbjct: 659 cgccggcgatagccggccttgtatccgtcgagtccattgatctgtcgtcaaattccctca 718

                                   
Query: 428 ccgggccgatcccgccagagctcg 451
           ||||  | |||||||| |||||||
Sbjct: 719 ccggcgcaatcccgccggagctcg 742



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>gb|AC087553.3| Oryza sativa (japonica cultivar-group) chromosome 5 clone P0685E10,
             complete sequence
          Length = 156772

 Score =  151 bits (76), Expect = 2e-33
 Identities = 172/204 (84%)
 Strand = Plus / Plus

                                                                         
Query: 248   tcctctccggctggtcgccggaggctgatgtctgctcctggcacggtgtttcatgcctga 307
             ||||||||||||||||||||||||| || || ||||| |||||||| ||  | |||||||
Sbjct: 41139 tcctctccggctggtcgccggaggccgacgtgtgctcatggcacggcgtgacgtgcctga 41198

                                                                         
Query: 308   caggagagggcattgtgacaggcctcaacttgtcgggatacggcctatcgggcaccttat 367
             |||| ||||||||||| || ||||||||| ||||||| |||||||| || |||||  |||
Sbjct: 41199 caggggagggcattgtcaccggcctcaacctgtcggggtacggcctgtccggcacgatat 41258

                                                                         
Query: 368   cgccggcgatagctggcctgatatccgtggagatcattgacctctcctccaactccctca 427
             ||||||||||||| |||||  ||||||| |||  |||||| || || || || |||||||
Sbjct: 41259 cgccggcgatagccggccttgtatccgtcgagtccattgatctgtcgtcaaattccctca 41318

                                     
Query: 428   ccgggccgatcccgccagagctcg 451
             ||||  | |||||||| |||||||
Sbjct: 41319 ccggcgcaatcccgccggagctcg 41342



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>gb|AC084817.5| Oryza sativa (japonica cultivar-group) chromosome 5 clone P0419C04,
              complete sequence
          Length = 136713

 Score =  151 bits (76), Expect = 2e-33
 Identities = 172/204 (84%)
 Strand = Plus / Plus

                                                                          
Query: 248    tcctctccggctggtcgccggaggctgatgtctgctcctggcacggtgtttcatgcctga 307
              ||||||||||||||||||||||||| || || ||||| |||||||| ||  | |||||||
Sbjct: 129354 tcctctccggctggtcgccggaggccgacgtgtgctcatggcacggcgtgacgtgcctga 129413

                                                                          
Query: 308    caggagagggcattgtgacaggcctcaacttgtcgggatacggcctatcgggcaccttat 367
              |||| ||||||||||| || ||||||||| ||||||| |||||||| || |||||  |||
Sbjct: 129414 caggggagggcattgtcaccggcctcaacctgtcggggtacggcctgtccggcacgatat 129473

                                                                          
Query: 368    cgccggcgatagctggcctgatatccgtggagatcattgacctctcctccaactccctca 427
              ||||||||||||| |||||  ||||||| |||  |||||| || || || || |||||||
Sbjct: 129474 cgccggcgatagccggccttgtatccgtcgagtccattgatctgtcgtcaaattccctca 129533

                                      
Query: 428    ccgggccgatcccgccagagctcg 451
              ||||  | |||||||| |||||||
Sbjct: 129534 ccggcgcaatcccgccggagctcg 129557



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>dbj|AP008211.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 5, complete
               sequence
          Length = 29737217

 Score =  151 bits (76), Expect = 2e-33
 Identities = 172/204 (84%)
 Strand = Plus / Plus

                                                                           
Query: 248     tcctctccggctggtcgccggaggctgatgtctgctcctggcacggtgtttcatgcctga 307
               ||||||||||||||||||||||||| || || ||||| |||||||| ||  | |||||||
Sbjct: 4154660 tcctctccggctggtcgccggaggccgacgtgtgctcatggcacggcgtgacgtgcctga 4154719

                                                                           
Query: 308     caggagagggcattgtgacaggcctcaacttgtcgggatacggcctatcgggcaccttat 367
               |||| ||||||||||| || ||||||||| ||||||| |||||||| || |||||  |||
Sbjct: 4154720 caggggagggcattgtcaccggcctcaacctgtcggggtacggcctgtccggcacgatat 4154779

                                                                           
Query: 368     cgccggcgatagctggcctgatatccgtggagatcattgacctctcctccaactccctca 427
               ||||||||||||| |||||  ||||||| |||  |||||| || || || || |||||||
Sbjct: 4154780 cgccggcgatagccggccttgtatccgtcgagtccattgatctgtcgtcaaattccctca 4154839

                                       
Query: 428     ccgggccgatcccgccagagctcg 451
               ||||  | |||||||| |||||||
Sbjct: 4154840 ccggcgcaatcccgccggagctcg 4154863



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>ref|NM_193792.1| Oryza sativa (japonica cultivar-group),  predicted mRNA
          Length = 2118

 Score = 44.1 bits (22), Expect = 0.39
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 406 gacctctcctccaactccctca 427
           ||||||||||||||||||||||
Sbjct: 442 gacctctcctccaactccctca 463



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>gb|AC153489.7| Mus musculus 10 BAC RP23-95A4 (Roswell Park Cancer Institute (C57BL/6J
             Female) Mouse BAC Library) complete sequence
          Length = 209653

 Score = 44.1 bits (22), Expect = 0.39
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                   
Query: 294   tgtttcatgcctgacaggagag 315
             ||||||||||||||||||||||
Sbjct: 32512 tgtttcatgcctgacaggagag 32491



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>gb|AC124360.5| Mus musculus BAC clone RP24-502M22 from chromosome 10, complete sequence
          Length = 184346

 Score = 44.1 bits (22), Expect = 0.39
 Identities = 22/22 (100%)
 Strand = Plus / Minus

                                    
Query: 294    tgtttcatgcctgacaggagag 315
              ||||||||||||||||||||||
Sbjct: 170584 tgtttcatgcctgacaggagag 170563



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>emb|AL355595.10| Human DNA sequence from clone RP11-276G3 on chromosome 13 Contains a
             novel gene and a novel pseudogene, complete sequence
          Length = 119343

 Score = 44.1 bits (22), Expect = 0.39
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                   
Query: 135   tctgctatctttgctctttggc 156
             ||||||||||||||||||||||
Sbjct: 38954 tctgctatctttgctctttggc 38975



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>dbj|AP008207.1| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, complete
                sequence
          Length = 43261740

 Score = 44.1 bits (22), Expect = 0.39
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                      
Query: 406      gacctctcctccaactccctca 427
                ||||||||||||||||||||||
Sbjct: 18201785 gacctctcctccaactccctca 18201806



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>dbj|AP003202.3| Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, BAC
             clone:B1075D06
          Length = 130528

 Score = 44.1 bits (22), Expect = 0.39
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                   
Query: 406   gacctctcctccaactccctca 427
             ||||||||||||||||||||||
Sbjct: 53230 gacctctcctccaactccctca 53251



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>emb|AL135818.3|CNS01DVH Human chromosome 14 DNA sequence BAC C-2547L24 of library CalTech-D from
              chromosome 14 of Homo sapiens (Human), complete sequence
          Length = 203777

 Score = 44.1 bits (22), Expect = 0.39
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                    
Query: 408    cctctcctccaactccctcacc 429
              ||||||||||||||||||||||
Sbjct: 149233 cctctcctccaactccctcacc 149254



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>emb|AL133153.3|CNS01DU3 Human chromosome 14 DNA sequence BAC R-895M11 of library RPCI-11 from
             chromosome 14 of Homo sapiens (Human), complete sequence
          Length = 231490

 Score = 44.1 bits (22), Expect = 0.39
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                   
Query: 408   cctctcctccaactccctcacc 429
             ||||||||||||||||||||||
Sbjct: 60781 cctctcctccaactccctcacc 60802



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>gb|AC124597.5| Mus musculus BAC clone RP23-21K1 from chromosome 6, complete sequence
          Length = 185242

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 269    aggctgatgtctgctcctgg 288
              ||||||||||||||||||||
Sbjct: 180113 aggctgatgtctgctcctgg 180132



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>gb|AF527417.1| Homo sapiens cell division cycle 25A (CDC25A) gene, complete cds
          Length = 31868

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 23    ttatacacatcttcccaact 42
             ||||||||||||||||||||
Sbjct: 29473 ttatacacatcttcccaact 29454



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>gb|AC125401.4| Mus musculus BAC clone RP24-538H16 from chromosome 6, complete sequence
          Length = 130679

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 269   aggctgatgtctgctcctgg 288
             ||||||||||||||||||||
Sbjct: 42699 aggctgatgtctgctcctgg 42718



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>gb|AC128673.4| Homo sapiens BAC clone RP11-1195P3 from UL, complete sequence
          Length = 209514

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 287    ggcacggtgtttcatgcctg 306
              ||||||||||||||||||||
Sbjct: 101763 ggcacggtgtttcatgcctg 101782



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>gb|AC164882.2| Mus musculus BAC clone RP23-88F16 from chromosome 9, complete sequence
          Length = 203020

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 410   tctcctccaactccctcacc 429
             ||||||||||||||||||||
Sbjct: 45486 tctcctccaactccctcacc 45505



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>emb|AL732314.18| Human DNA sequence from clone RP13-465B17 on chromosome X Contains the 5'
              end of the gene for protein phosphatase 2A 48 kDa
              regulatory subunit (PR48), a fatty acid binding protein 5
              (psoriasis-associated) (FABP5) pseudogene, a pseudogene
              similar to part of keratin 18 (KRT18) and nine CpG islands,
              complete sequence
          Length = 218723

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 287    ggcacggtgtttcatgcctg 306
              ||||||||||||||||||||
Sbjct: 121347 ggcacggtgtttcatgcctg 121328



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>emb|AL360270.18| Human DNA sequence from clone RP11-96K19 on chromosome 1 Contains a
             putative novel transcript, a gene for a novel protein
             (FLJ11269), a processed pseudogene for cyclin T2 (CCNT2)
             and a CpG island, complete sequence
          Length = 172805

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 287   ggcacggtgtttcatgcctg 306
             ||||||||||||||||||||
Sbjct: 68034 ggcacggtgtttcatgcctg 68015



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>emb|AL353802.14| Human DNA sequence from clone RP4-662P1 on chromosome 1p31.3-32.3
             Contains part of the gene for InaD-like protein (INADL),
             complete sequence
          Length = 143226

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 287   ggcacggtgtttcatgcctg 306
             ||||||||||||||||||||
Sbjct: 93268 ggcacggtgtttcatgcctg 93249



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>emb|AL138741.13| Human DNA sequence from clone RP3-516D24 on chromosome 6, complete
             sequence
          Length = 67372

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 287   ggcacggtgtttcatgcctg 306
             ||||||||||||||||||||
Sbjct: 65144 ggcacggtgtttcatgcctg 65125



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>gb|BC039099.1| Homo sapiens cDNA clone IMAGE:4824737
          Length = 1264

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 134 ttctgctatctttgctcttt 153
           ||||||||||||||||||||
Sbjct: 257 ttctgctatctttgctcttt 276



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>gb|AC175824.2| Pan troglodytes BAC clone CH251-171H18 from chromosome unknown,
            complete sequence
          Length = 146069

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                
Query: 287  ggcacggtgtttcatgcctg 306
            ||||||||||||||||||||
Sbjct: 7985 ggcacggtgtttcatgcctg 7966



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>dbj|AK092143.1| Homo sapiens cDNA FLJ34824 fis, clone NT2NE2008783
          Length = 2078

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 405 tgacctctcctccaactccc 424
           ||||||||||||||||||||
Sbjct: 836 tgacctctcctccaactccc 855



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>gb|AC109584.2| Homo sapiens chromosome 3 clone RP11-674P14, complete sequence
          Length = 179199

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                  
Query: 405    tgacctctcctccaactccc 424
              ||||||||||||||||||||
Sbjct: 131406 tgacctctcctccaactccc 131387



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>gb|AC011773.9| Homo sapiens chromosome 8, clone RP11-219B4, complete sequence
          Length = 160283

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 134   ttctgctatctttgctcttt 153
             ||||||||||||||||||||
Sbjct: 79245 ttctgctatctttgctcttt 79226



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>gb|AC074135.3| Homo sapiens chromosome 19 clone CTD-2291D10, complete sequence
          Length = 121050

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 287   ggcacggtgtttcatgcctg 306
             ||||||||||||||||||||
Sbjct: 74307 ggcacggtgtttcatgcctg 74326



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>dbj|AP007155.1| Aspergillus oryzae RIB40 genomic DNA, SC003
          Length = 4170692

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 408    cctctcctccaactccctca 427
              ||||||||||||||||||||
Sbjct: 479862 cctctcctccaactccctca 479881



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>gb|AC092797.2| Homo sapiens chromosome 3 clone RP11-452E8, complete sequence
          Length = 200278

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Minus

                                 
Query: 405   tgacctctcctccaactccc 424
             ||||||||||||||||||||
Sbjct: 83286 tgacctctcctccaactccc 83267



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>gb|AC092290.3| Homo sapiens chromosome 5 clone CTD-2376K3, complete sequence
          Length = 80527

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 287   ggcacggtgtttcatgcctg 306
             ||||||||||||||||||||
Sbjct: 62879 ggcacggtgtttcatgcctg 62898



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>gb|AC161621.5| Pan troglodytes BAC clone CH251-164P18 from chromosome unknown,
             complete sequence
          Length = 172032

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 287   ggcacggtgtttcatgcctg 306
             ||||||||||||||||||||
Sbjct: 56970 ggcacggtgtttcatgcctg 56989



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>gb|AC124916.3| Homo sapiens chromosome 3 clone RP11-1029M24, complete sequence
          Length = 207596

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                  
Query: 23     ttatacacatcttcccaact 42
              ||||||||||||||||||||
Sbjct: 184346 ttatacacatcttcccaact 184365



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>gb|AC104190.3| Homo sapiens chromosome 3 clone RP11-502L5, complete sequence
          Length = 171181

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                                 
Query: 23    ttatacacatcttcccaact 42
             ||||||||||||||||||||
Sbjct: 48599 ttatacacatcttcccaact 48618



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>dbj|AP006564.1| Homo sapiens genomic DNA, chromosome 18 clone:RP11-1157N02_A, complete
             sequence
          Length = 127500

 Score = 40.1 bits (20), Expect = 6.1
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                     
Query: 283   tcctggcacggtgtttcatgcctg 306
             ||||||||||||| ||||||||||
Sbjct: 74219 tcctggcacggtggttcatgcctg 74242


  Database: nt
    Posted date:  May 29, 2006 11:10 AM
  Number of letters in database: 3,984,495,279
  Number of sequences in database:  917,343
  
  Database: /shigen/export/home/twatanab/db/nt/nt.01
    Posted date:  May 29, 2006 11:16 AM
  Number of letters in database: 3,988,174,986
  Number of sequences in database:  835,257
  
  Database: /shigen/export/home/twatanab/db/nt/nt.02
    Posted date:  May 29, 2006 11:21 AM
  Number of letters in database: 3,991,246,324
  Number of sequences in database:  771,481
  
  Database: /shigen/export/home/twatanab/db/nt/nt.03
    Posted date:  May 29, 2006 11:27 AM
  Number of letters in database: 3,990,718,311
  Number of sequences in database:  977,174
  
  Database: /shigen/export/home/twatanab/db/nt/nt.04
    Posted date:  May 29, 2006 11:29 AM
  Number of letters in database: 1,278,410,368
  Number of sequences in database:  400,813
  
Lambda     K      H
    1.37    0.711     1.31 

Gapped
Lambda     K      H
    1.37    0.711     1.31 


Matrix: blastn matrix:1 -3
Gap Penalties: Existence: 5, Extension: 2
Number of Hits to DB: 2,881,138
Number of Sequences: 3902068
Number of extensions: 2881138
Number of successful extensions: 52256
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 34
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 52134
Number of HSP's gapped (non-prelim): 122
length of query: 451
length of database: 17,233,045,268
effective HSP length: 22
effective length of query: 429
effective length of database: 17,147,199,772
effective search space: 7356148702188
effective search space used: 7356148702188
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 15 (29.7 bits)
S1: 12 (24.3 bits)
S2: 20 (40.1 bits)
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