ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl40e03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1MFPA_CUCSA (Q39659) Glyoxysomal fatty acid beta-oxidation multif... 69 5e-12
2MFPA_BRANA (O49809) Glyoxysomal fatty acid beta-oxidation multif... 64 1e-10
3MFP_ORYSA (Q8W1L6) Peroxisomal fatty acid beta-oxidation multifu... 47 2e-05
4D3D2_HUMAN (P42126) 3,2-trans-enoyl-CoA isomerase, mitochondrial... 34 0.13
5D3D2_MOUSE (P42125) 3,2-trans-enoyl-CoA isomerase, mitochondrial... 32 0.49
6ECHP_CAVPO (P55100) Peroxisomal bifunctional enzyme (PBE) (PBFE)... 31 1.4
7PURL_LACCA (P35852) Phosphoribosylformylglycinamidine synthase I... 29 5.4
8ECHP_MOUSE (Q9DBM2) Peroxisomal bifunctional enzyme (PBE) (PBFE)... 28 7.1
9HIW_DROME (Q9NB71) Ubiquitin ligase protein highwire (EC 6.3.2.-... 28 9.2
10ECHP_RAT (P07896) Peroxisomal bifunctional enzyme (PBE) (PBFE) [... 28 9.2
11D3D2_RAT (P23965) 3,2-trans-enoyl-CoA isomerase, mitochondrial p... 28 9.2
12PREB_RAT (Q9WTV0) Prolactin regulatory element-binding protein (... 28 9.2

>MFPA_CUCSA (Q39659) Glyoxysomal fatty acid beta-oxidation multifunctional|
           protein MFP-a [Includes: Enoyl-CoA hydratase (EC
           4.2.1.17); 3-2-trans-enoyl-CoA isomerase (EC 5.3.3.8);
           3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3);
           3-hydroxyacyl-CoA dehydrogenase
          Length = 725

 Score = 68.9 bits (167), Expect = 5e-12
 Identities = 32/48 (66%), Positives = 40/48 (83%)
 Frame = +3

Query: 285 GSMAKGRSEMEVGPDGVAVIYVANPPVNALSMDVMYSLKGHYEEALRR 428
           GS AKGR+ MEVG DGVA+I + NPPVN+LS DV++SL+  YE+ALRR
Sbjct: 2   GSNAKGRTVMEVGTDGVAIITIINPPVNSLSFDVLFSLRDSYEQALRR 49



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>MFPA_BRANA (O49809) Glyoxysomal fatty acid beta-oxidation multifunctional|
           protein MFP-a [Includes: Enoyl-CoA hydratase (EC
           4.2.1.17); 3-2-trans-enoyl-CoA isomerase (EC 5.3.3.8);
           3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3);
           3-hydroxyacyl-CoA dehydrogenase
          Length = 725

 Score = 64.3 bits (155), Expect = 1e-10
 Identities = 31/47 (65%), Positives = 37/47 (78%)
 Frame = +3

Query: 288 SMAKGRSEMEVGPDGVAVIYVANPPVNALSMDVMYSLKGHYEEALRR 428
           S  KG + +EVG DGVAVI + NPPVN+LS DV+YSLK +YEEAL R
Sbjct: 3   SRTKGTTTIEVGADGVAVITLINPPVNSLSFDVLYSLKSNYEEALSR 49



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>MFP_ORYSA (Q8W1L6) Peroxisomal fatty acid beta-oxidation multifunctional|
           protein (MFP) [Includes: Enoyl-CoA hydratase (EC
           4.2.1.17); 3-2-trans-enoyl-CoA isomerase (EC 5.3.3.8);
           3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3);
           3-hydroxyacyl-CoA dehydrogenase
          Length = 725

 Score = 47.0 bits (110), Expect = 2e-05
 Identities = 23/42 (54%), Positives = 28/42 (66%)
 Frame = +3

Query: 303 RSEMEVGPDGVAVIYVANPPVNALSMDVMYSLKGHYEEALRR 428
           R  MEVG DGVAV+ + NPPVNAL   ++  LK  Y EA+ R
Sbjct: 6   RVTMEVGADGVAVVTICNPPVNALHPIIIQGLKEKYAEAMDR 47



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>D3D2_HUMAN (P42126) 3,2-trans-enoyl-CoA isomerase, mitochondrial precursor (EC|
           5.3.3.8) (Dodecenoyl-CoA isomerase)
           (Delta(3),delta(2)-enoyl-CoA isomerase) (D3,D2-enoyl-CoA
           isomerase)
          Length = 302

 Score = 34.3 bits (77), Expect = 0.13
 Identities = 22/52 (42%), Positives = 29/52 (55%), Gaps = 5/52 (9%)
 Frame = +3

Query: 258 GRDPRKRKGGSMAK--GRSEMEVGPD---GVAVIYVANPPVNALSMDVMYSL 398
           GR  R   GG  A+  G   + V PD   GVAV+   NPPVN+LS++ +  L
Sbjct: 27  GRTERAAGGGDGARRFGSQRVLVEPDAGAGVAVMKFKNPPVNSLSLEFLTEL 78



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>D3D2_MOUSE (P42125) 3,2-trans-enoyl-CoA isomerase, mitochondrial precursor (EC|
           5.3.3.8) (Dodecenoyl-CoA isomerase)
           (Delta(3),delta(2)-enoyl-CoA isomerase) (D3,D2-enoyl-CoA
           isomerase)
          Length = 289

 Score = 32.3 bits (72), Expect = 0.49
 Identities = 13/23 (56%), Positives = 19/23 (82%)
 Frame = +3

Query: 321 GPDGVAVIYVANPPVNALSMDVM 389
           GP GVAV+ + NPPVN+LS++ +
Sbjct: 40  GPAGVAVMKLRNPPVNSLSLECL 62



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>ECHP_CAVPO (P55100) Peroxisomal bifunctional enzyme (PBE) (PBFE) [Includes:|
           Enoyl-CoA hydratase (EC 4.2.1.17); 3,2-trans-enoyl-CoA
           isomerase (EC 5.3.3.8); 3-hydroxyacyl-CoA dehydrogenase
           (EC 1.1.1.35)]
          Length = 725

 Score = 30.8 bits (68), Expect = 1.4
 Identities = 13/33 (39%), Positives = 23/33 (69%)
 Frame = +3

Query: 324 PDGVAVIYVANPPVNALSMDVMYSLKGHYEEAL 422
           P  +A+I + NPPVNA+S  V++ +K   ++A+
Sbjct: 7   PHSLALIRLRNPPVNAISPAVIHGIKEGLQKAM 39



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>PURL_LACCA (P35852) Phosphoribosylformylglycinamidine synthase II (EC 6.3.5.3)|
           (FGAM synthase II)
          Length = 740

 Score = 28.9 bits (63), Expect = 5.4
 Identities = 18/47 (38%), Positives = 23/47 (48%)
 Frame = +3

Query: 282 GGSMAKGRSEMEVGPDGVAVIYVANPPVNALSMDVMYSLKGHYEEAL 422
           G S A G    E   D  AV  V +P +  L MD    + GH++EAL
Sbjct: 226 GASFASGDFSDEEAADRSAV-QVGDPFMEKLLMDACLEITGHHQEAL 271



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>ECHP_MOUSE (Q9DBM2) Peroxisomal bifunctional enzyme (PBE) (PBFE) [Includes:|
           Enoyl-CoA hydratase (EC 4.2.1.17); 3,2-trans-enoyl-CoA
           isomerase (EC 5.3.3.8); 3-hydroxyacyl-CoA dehydrogenase
           (EC 1.1.1.35)]
          Length = 717

 Score = 28.5 bits (62), Expect = 7.1
 Identities = 12/32 (37%), Positives = 21/32 (65%)
 Frame = +3

Query: 324 PDGVAVIYVANPPVNALSMDVMYSLKGHYEEA 419
           P  +A+I + NPPVNA+S  V+  ++   ++A
Sbjct: 7   PHSLAMIRLCNPPVNAISPTVITEVRNGLQKA 38



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>HIW_DROME (Q9NB71) Ubiquitin ligase protein highwire (EC 6.3.2.-) (Protein|
            pam/highwire/rpm-1)
          Length = 5233

 Score = 28.1 bits (61), Expect = 9.2
 Identities = 21/64 (32%), Positives = 30/64 (46%)
 Frame = +3

Query: 237  PCERTSAGRDPRKRKGGSMAKGRSEMEVGPDGVAVIYVANPPVNALSMDVMYSLKGHYEE 416
            P    SAG  PR R+GG   +G S  E  P         +PP  A +MD  Y++   ++ 
Sbjct: 1295 PGTPVSAGSVPRSRRGGK--QGTSSPEPIP---------SPPQLAFTMDPTYNVLWVFDG 1343

Query: 417  ALRR 428
            A R+
Sbjct: 1344 AARK 1347



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>ECHP_RAT (P07896) Peroxisomal bifunctional enzyme (PBE) (PBFE) [Includes:|
           Enoyl-CoA hydratase (EC 4.2.1.17); 3,2-trans-enoyl-CoA
           isomerase (EC 5.3.3.8); 3-hydroxyacyl-CoA dehydrogenase
           (EC 1.1.1.35)]
          Length = 721

 Score = 28.1 bits (61), Expect = 9.2
 Identities = 12/32 (37%), Positives = 21/32 (65%)
 Frame = +3

Query: 324 PDGVAVIYVANPPVNALSMDVMYSLKGHYEEA 419
           P  +A+I + NPPVNA+S  V+  ++   ++A
Sbjct: 7   PHSLAMIRLCNPPVNAVSPTVIREVRNGLQKA 38



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>D3D2_RAT (P23965) 3,2-trans-enoyl-CoA isomerase, mitochondrial precursor (EC|
           5.3.3.8) (Dodecenoyl-CoA isomerase)
           (Delta(3),delta(2)-enoyl-CoA isomerase) (D3,D2-enoyl-CoA
           isomerase)
          Length = 289

 Score = 28.1 bits (61), Expect = 9.2
 Identities = 11/23 (47%), Positives = 17/23 (73%)
 Frame = +3

Query: 321 GPDGVAVIYVANPPVNALSMDVM 389
           G  G+AV+   NPPVN+LS++ +
Sbjct: 40  GEAGIAVMKFKNPPVNSLSLEFL 62



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>PREB_RAT (Q9WTV0) Prolactin regulatory element-binding protein (Mammalian|
           guanine nucleotide exchange factor mSec12)
          Length = 417

 Score = 28.1 bits (61), Expect = 9.2
 Identities = 13/28 (46%), Positives = 19/28 (67%)
 Frame = +3

Query: 252 SAGRDPRKRKGGSMAKGRSEMEVGPDGV 335
           S  + PR+RKG + A+ +S  EV P+GV
Sbjct: 110 SKEQGPRQRKGAAPAEKKSGAEVHPEGV 137


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 41,416,670
Number of Sequences: 219361
Number of extensions: 658808
Number of successful extensions: 1967
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1924
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1967
length of database: 80,573,946
effective HSP length: 101
effective length of database: 58,418,485
effective search space used: 2395157885
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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