ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl38h12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1Y5843_ARATH (Q9FT92) Protein At5g08430 60 2e-09
2VPS3_YEAST (P23643) Vacuolar protein sorting-associated protein ... 31 1.4
3SYD_LEPIN (Q8F5K1) Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspar... 31 1.4
4SYD_LEPIC (Q72QK5) Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspar... 31 1.4
5HPSE2_ARATH (Q8L608) Heparanase-like protein 2 precursor (EC 3.2... 30 1.8
6MURD_PARUW (Q6MBS4) UDP-N-acetylmuramoylalanine--D-glutamate lig... 30 3.0
7CH60_LEIXX (Q6ADK2) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 29 3.9
8RS16_SCHPO (O60144) 40S ribosomal protein S16 28 6.7
9F13A_RAT (O08619) Coagulation factor XIII A chain precursor (EC ... 28 6.7
10TETS_LACLA (Q48712) Tetracycline resistance protein tetS (Tet(S)) 28 8.8
11TETS_LISMO (Q48791) Tetracycline resistance protein tetS (Tet(S)) 28 8.8
12GATA_BACST (Q93LE2) Glutamyl-tRNA(Gln) amidotransferase subunit ... 28 8.8

>Y5843_ARATH (Q9FT92) Protein At5g08430|
          Length = 553

 Score = 60.5 bits (145), Expect = 2e-09
 Identities = 45/140 (32%), Positives = 67/140 (47%), Gaps = 1/140 (0%)
 Frame = +1

Query: 7   KSRKYDQRALQTNLDDFAAIDNHNIGLIYLRRNLVEELIGDADTFSEKVLGAFVRIRISG 186
           K  K   + ++     FAAI + NI L+YLR++LV+EL+   DTF  K+LG+FVRI+   
Sbjct: 132 KIAKRTSKVVKKPRGTFAAIVSDNIKLLYLRKSLVQELLKSPDTFEGKMLGSFVRIKSDP 191

Query: 187 TGQRQDI-YRLVQIVGTGTAAEKYKCGKKTTDITLEILNLDKKEVITIDITSXQXXXXXX 363
               Q   Y+LVQ+ G        K    T D  L++ N  K   ++I + S        
Sbjct: 192 NDYLQKYPYQLVQVTGV-------KKEHGTDDFLLQVTNYVKD--VSISVLSDDNFSQEE 242

Query: 364 XXXXXXSIKCGFISRLTVGE 423
                  IK G + + T+ E
Sbjct: 243 CEDLHQRIKNGLLKKPTIVE 262



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>VPS3_YEAST (P23643) Vacuolar protein sorting-associated protein VPS3|
          Length = 1011

 Score = 30.8 bits (68), Expect = 1.4
 Identities = 16/34 (47%), Positives = 20/34 (58%)
 Frame = +1

Query: 232 TGTAAEKYKCGKKTTDITLEILNLDKKEVITIDI 333
           TG     +    KT DITL  L L+KKE IT+D+
Sbjct: 610 TGLLTNDFDDIMKTIDITLFKLRLEKKETITVDM 643



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>SYD_LEPIN (Q8F5K1) Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspartate--tRNA|
           ligase) (AspRS)
          Length = 601

 Score = 30.8 bits (68), Expect = 1.4
 Identities = 22/73 (30%), Positives = 39/73 (53%)
 Frame = +1

Query: 112 EELIGDADTFSEKVLGAFVRIRISGTGQRQDIYRLVQIVGTGTAAEKYKCGKKTTDITLE 291
           +EL+GD+ T +EKV   +V + + GT +++D+  +   + TGT         +     LE
Sbjct: 60  KELLGDSFTLAEKVRSEYV-LAVRGTLKKRDLESINPRMQTGTI--------EVVLDQLE 110

Query: 292 ILNLDKKEVITID 330
           ILN+ K    ++D
Sbjct: 111 ILNVAKTPPFSLD 123



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>SYD_LEPIC (Q72QK5) Aspartyl-tRNA synthetase (EC 6.1.1.12) (Aspartate--tRNA|
           ligase) (AspRS)
          Length = 601

 Score = 30.8 bits (68), Expect = 1.4
 Identities = 22/73 (30%), Positives = 39/73 (53%)
 Frame = +1

Query: 112 EELIGDADTFSEKVLGAFVRIRISGTGQRQDIYRLVQIVGTGTAAEKYKCGKKTTDITLE 291
           +EL+GD+ T +EKV   +V + + GT +++D+  +   + TGT         +     LE
Sbjct: 60  KELLGDSFTLAEKVRSEYV-LAVRGTLKKRDLESINPRMQTGTI--------EVVLDQLE 110

Query: 292 ILNLDKKEVITID 330
           ILN+ K    ++D
Sbjct: 111 ILNVAKTPPFSLD 123



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>HPSE2_ARATH (Q8L608) Heparanase-like protein 2 precursor (EC 3.2.-.-)|
          Length = 539

 Score = 30.4 bits (67), Expect = 1.8
 Identities = 28/107 (26%), Positives = 45/107 (42%), Gaps = 9/107 (8%)
 Frame = +1

Query: 46  LDDFAAIDNHNIGLIYLRRNLVEELIG--DADTF--SEKVLGAFVRIRISGTGQRQDIYR 213
           LD       HN   +Y R+ LV    G  + +TF  +     A +  R+ G G       
Sbjct: 339 LDQLGISSKHNTK-VYCRQALVGGFYGLLEKETFVPNPDYYSALLWHRLMGKG------- 390

Query: 214 LVQIVGTGTAAEKY-----KCGKKTTDITLEILNLDKKEVITIDITS 339
              I+G  T A +Y      C K+   IT+ ++NL K    T+ +++
Sbjct: 391 ---ILGVQTTASEYLRAYVHCSKRRAGITILLINLSKHTTFTVAVSN 434



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>MURD_PARUW (Q6MBS4) UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC|
           6.3.2.9) (UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
           synthetase) (D-glutamic acid-adding enzyme)
          Length = 444

 Score = 29.6 bits (65), Expect = 3.0
 Identities = 15/58 (25%), Positives = 29/58 (50%)
 Frame = +1

Query: 172 IRISGTGQRQDIYRLVQIVGTGTAAEKYKCGKKTTDITLEILNLDKKEVITIDITSXQ 345
           I ++GT  +  +  L+  +      +    G   T +T EIL LD +E+  ++++S Q
Sbjct: 114 IGVTGTNGKTTVTLLITHILNANGLKAKALGNVGTPLTREILTLDFQEIAVLELSSYQ 171



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>CH60_LEIXX (Q6ADK2) 60 kDa chaperonin (Protein Cpn60) (groEL protein)|
          Length = 539

 Score = 29.3 bits (64), Expect = 3.9
 Identities = 23/82 (28%), Positives = 39/82 (47%), Gaps = 4/82 (4%)
 Frame = +1

Query: 106 LVEELIGDA--DTFSEKVLGAF--VRIRISGTGQRQDIYRLVQIVGTGTAAEKYKCGKKT 273
           + E++ G+A       K+ G F  V ++  G G R+        + TG      + G K 
Sbjct: 248 IAEDVDGEALATLIVNKIRGIFKSVAVKAPGFGDRRKAMLQDIAILTGGQVISEEVGLKL 307

Query: 274 TDITLEILNLDKKEVITIDITS 339
            ++TL++L   +K VIT D T+
Sbjct: 308 ENVTLDLLGKARKVVITKDETT 329



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>RS16_SCHPO (O60144) 40S ribosomal protein S16|
          Length = 140

 Score = 28.5 bits (62), Expect = 6.7
 Identities = 22/73 (30%), Positives = 32/73 (43%)
 Frame = +1

Query: 118 LIGDADTFSEKVLGAFVRIRISGTGQRQDIYRLVQIVGTGTAAEKYKCGKKTTDITLEIL 297
           L+  AD F+    G  +R+R+SG G    IY + Q +     A   K   + +   L   
Sbjct: 50  LVAGADKFA----GVDIRVRVSGGGHVSQIYAIRQAISKAIVAYYQKFVDEHSKAEL--- 102

Query: 298 NLDKKEVITIDIT 336
              KK +IT D T
Sbjct: 103 ---KKALITYDRT 112



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>F13A_RAT (O08619) Coagulation factor XIII A chain precursor (EC 2.3.2.13)|
           (Coagulation factor XIIIa) (Protein-glutamine
           gamma-glutamyltransferase A chain) (Transglutaminase A
           chain)
          Length = 731

 Score = 28.5 bits (62), Expect = 6.7
 Identities = 17/63 (26%), Positives = 36/63 (57%), Gaps = 6/63 (9%)
 Frame = +1

Query: 151 VLGAFVRIRISGTGQRQDIYRLVQIVG------TGTAAEKYKCGKKTTDITLEILNLDKK 312
           VLG   R+ I+      ++Y ++  +       TG + +++K   ++ ++TL+ L+L+KK
Sbjct: 528 VLGKDFRVTITFQNNSSNLYTILAYLSGNITFYTGVSKKEFKT--ESFEVTLDPLSLEKK 585

Query: 313 EVI 321
           EV+
Sbjct: 586 EVL 588



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>TETS_LACLA (Q48712) Tetracycline resistance protein tetS (Tet(S))|
          Length = 646

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 25/89 (28%), Positives = 37/89 (41%), Gaps = 8/89 (8%)
 Frame = +1

Query: 73  HNIGLIYLRRNLVEELIGDADTFSEKVLGAFVRIRISGTGQRQDIYRLVQIVGT------ 234
           +NIG+  L   +  +L       S+K+ G   ++  S  GQR    RL    GT      
Sbjct: 228 NNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLYS--GTLHLRDS 285

Query: 235 --GTAAEKYKCGKKTTDITLEILNLDKKE 315
              +  EK K  +  T I  E+  +DK E
Sbjct: 286 VNISEKEKIKVTEMYTSINGELRQIDKAE 314



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>TETS_LISMO (Q48791) Tetracycline resistance protein tetS (Tet(S))|
          Length = 641

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 25/89 (28%), Positives = 37/89 (41%), Gaps = 8/89 (8%)
 Frame = +1

Query: 73  HNIGLIYLRRNLVEELIGDADTFSEKVLGAFVRIRISGTGQRQDIYRLVQIVGT------ 234
           +NIG+  L   +  +L       S+K+ G   ++  S  GQR    RL    GT      
Sbjct: 223 NNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLYS--GTLHLRDS 280

Query: 235 --GTAAEKYKCGKKTTDITLEILNLDKKE 315
              +  EK K  +  T I  E+  +DK E
Sbjct: 281 VNISEKEKIKVTEMYTSINGELRQIDKAE 309



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>GATA_BACST (Q93LE2) Glutamyl-tRNA(Gln) amidotransferase subunit A (EC 6.3.5.-)|
           (Glu-ADT subunit A)
          Length = 485

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 18/66 (27%), Positives = 29/66 (43%)
 Frame = +1

Query: 223 IVGTGTAAEKYKCGKKTTDITLEILNLDKKEVITIDITSXQXXXXXXXXXXXXSIKCGFI 402
           I+G  T    +K G+KT+D     L +   +++TI +                S+ CGF+
Sbjct: 399 IIGPTTPTPAFKIGEKTSD----PLTMYANDILTIPVN--------LAGVPAISVPCGFV 446

Query: 403 SRLTVG 420
             L VG
Sbjct: 447 DGLPVG 452


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,648,724
Number of Sequences: 219361
Number of extensions: 973527
Number of successful extensions: 2500
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2478
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2500
length of database: 80,573,946
effective HSP length: 101
effective length of database: 58,418,485
effective search space used: 2278320915
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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