ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl38h07
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AMY_THECU (P29750) Alpha-amylase precursor (EC 3.2.1.1) (1,4-alp... 45 9e-05
2AMYG_ASPOR (P36914) Glucoamylase precursor (EC 3.2.1.3) (Glucan ... 36 0.043
3AMY_STRLM (P09794) Alpha-amylase precursor (EC 3.2.1.1) (1,4-alp... 36 0.056
4AMY_STRGR (P30270) Alpha-amylase precursor (EC 3.2.1.1) (1,4-alp... 36 0.056
5CDGT_KLEOX (P08704) Cyclomaltodextrin glucanotransferase precurs... 33 0.28
6AMYG_NEUCR (P14804) Glucoamylase precursor (EC 3.2.1.3) (Glucan ... 33 0.36
7K1434_HUMAN (Q9NPB8) Protein KIAA1434 32 0.62
8YOR1_BHV1S (Q08104) Hypothetical 16.1 kDa protein (ORF1) 30 4.0
9RPOD_PSEPU (P52327) RNA polymerase sigma factor rpoD (Sigma-70) 30 4.0
10RT06_DROME (Q9VZD5) Probable mitochondrial 28S ribosomal protein... 29 5.2
11QUIA_XANCJ (Q9XD78) Probable quinate dehydrogenase [Pyrroloquino... 29 5.2
12APBE_CHLMU (Q9PKW2) Thiamine biosynthesis lipoprotein apbE precu... 29 5.2
13RHO_STRLI (P52157) Transcription termination factor rho (EC 3.6.... 29 6.8
14LEUD1_DEIRA (Q9RTY5) 3-isopropylmalate dehydratase small subunit... 28 8.9

>AMY_THECU (P29750) Alpha-amylase precursor (EC 3.2.1.1) (1,4-alpha-D-glucan|
           glucanohydrolase)
          Length = 605

 Score = 45.1 bits (105), Expect = 9e-05
 Identities = 24/60 (40%), Positives = 31/60 (51%)
 Frame = +2

Query: 263 STVTVIFKLPYYTQWGQSLVIAGSAPALGSWSVKQGLSLSPVHQGNALVWCGQVSVAAGL 442
           +TVT  F     T +GQ + + GS P LGSW   QG+ L     G   VW G V + AG+
Sbjct: 504 TTVTARFHATVTTWYGQEVAVVGSIPELGSWQPAQGVRLR-TDSGTYPVWSGAVDLPAGV 562



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>AMYG_ASPOR (P36914) Glucoamylase precursor (EC 3.2.1.3) (Glucan|
           1,4-alpha-glucosidase) (1,4-alpha-D-glucan
           glucohydrolase)
          Length = 612

 Score = 36.2 bits (82), Expect = 0.043
 Identities = 19/60 (31%), Positives = 34/60 (56%), Gaps = 1/60 (1%)
 Frame = +2

Query: 263 STVTVIFKLPYYTQWGQSLVIAGSAPALGSWSVKQGLSL-SPVHQGNALVWCGQVSVAAG 439
           +TV+V F +   T +G+S+ I GS   LGSW+     +L +  +  +  +W G +++ AG
Sbjct: 510 TTVSVTFAVKATTVYGESIKIVGSISQLGSWNPSSATALNADSYTTDNPLWTGTINLPAG 569



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>AMY_STRLM (P09794) Alpha-amylase precursor (EC 3.2.1.1) (1,4-alpha-D-glucan|
           glucanohydrolase)
          Length = 566

 Score = 35.8 bits (81), Expect = 0.056
 Identities = 19/61 (31%), Positives = 28/61 (45%)
 Frame = +2

Query: 257 GKSTVTVIFKLPYYTQWGQSLVIAGSAPALGSWSVKQGLSLSPVHQGNALVWCGQVSVAA 436
           G    +  F +   T WG+++ + G   ALG+W   + L L P       VW   V +AA
Sbjct: 467 GTGQTSASFHVNATTAWGENIYVTGDQAALGNWDPARALKLDPAAYP---VWKLDVPLAA 523

Query: 437 G 439
           G
Sbjct: 524 G 524



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>AMY_STRGR (P30270) Alpha-amylase precursor (EC 3.2.1.1) (1,4-alpha-D-glucan|
           glucanohydrolase)
          Length = 566

 Score = 35.8 bits (81), Expect = 0.056
 Identities = 19/61 (31%), Positives = 28/61 (45%)
 Frame = +2

Query: 257 GKSTVTVIFKLPYYTQWGQSLVIAGSAPALGSWSVKQGLSLSPVHQGNALVWCGQVSVAA 436
           G    +  F +   T WG+++ + G   ALG+W   + L L P       VW   V +AA
Sbjct: 467 GTGQTSASFHVNATTAWGENIYVTGDQAALGNWDPARALKLDPAAYP---VWKLDVPLAA 523

Query: 437 G 439
           G
Sbjct: 524 G 524



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>CDGT_KLEOX (P08704) Cyclomaltodextrin glucanotransferase precursor (EC|
           2.4.1.19) (Cyclodextrin-glycosyltransferase) (CGTase)
          Length = 655

 Score = 33.5 bits (75), Expect = 0.28
 Identities = 16/51 (31%), Positives = 26/51 (50%)
 Frame = +2

Query: 296 YTQWGQSLVIAGSAPALGSWSVKQGLSLSPVHQGNALVWCGQVSVAAGLHV 448
           YT  GQS+ I G+ P LG W + + + +SP        W   + + + L+V
Sbjct: 570 YTISGQSVYIIGNIPQLGGWDLTKAVKISPTQYPQ---WSASLELPSDLNV 617



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>AMYG_NEUCR (P14804) Glucoamylase precursor (EC 3.2.1.3) (Glucan|
           1,4-alpha-glucosidase) (1,4-alpha-D-glucan
           glucohydrolase)
          Length = 626

 Score = 33.1 bits (74), Expect = 0.36
 Identities = 15/38 (39%), Positives = 23/38 (60%)
 Frame = +2

Query: 269 VTVIFKLPYYTQWGQSLVIAGSAPALGSWSVKQGLSLS 382
           V V F     T +GQ++ + GS  ALG+W+   G++LS
Sbjct: 526 VLVTFNEKVTTSYGQTVKVVGSIAALGNWAPASGVTLS 563



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>K1434_HUMAN (Q9NPB8) Protein KIAA1434|
          Length = 672

 Score = 32.3 bits (72), Expect = 0.62
 Identities = 14/48 (29%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
 Frame = +2

Query: 308 GQSLVIAGSAPALGSWSVKQGLSLSPVHQ-GNALVWCGQVSVAAGLHV 448
           G+   I GS  ALG+W+ +  ++L P +  G +++W   + ++ G+ V
Sbjct: 17  GEVFAICGSCDALGNWNPQNAVALLPENDTGESMLWKATIVLSRGVSV 64



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>YOR1_BHV1S (Q08104) Hypothetical 16.1 kDa protein (ORF1)|
          Length = 148

 Score = 29.6 bits (65), Expect = 4.0
 Identities = 29/82 (35%), Positives = 38/82 (46%), Gaps = 9/82 (10%)
 Frame = -1

Query: 457 SCTHM*AGGDRNLATPDQRVPLVNRAQGQPLL---DAPG--PERRRGAGDDEALP----P 305
           +C H    G   +  PD   P+V     +PLL    APG  P+R  GA   E +P    P
Sbjct: 43  ACRHEQEDGLYAMLPPDY-FPVV--PSSKPLLVKVPAPGASPDRTGGAVHFECVPAPRRP 99

Query: 304 LRVVRQLEDHGHRALPHLFSEQ 239
           L+  RQL D     LPH F ++
Sbjct: 100 LQFFRQLYDGTFVKLPHNFPDE 121



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>RPOD_PSEPU (P52327) RNA polymerase sigma factor rpoD (Sigma-70)|
          Length = 614

 Score = 29.6 bits (65), Expect = 4.0
 Identities = 20/71 (28%), Positives = 28/71 (39%), Gaps = 1/71 (1%)
 Frame = -1

Query: 430 DRNLATPDQRVPLVNRAQGQPLLDAPGPERRRGAGDDEALPPLRVVRQLEDHGHRALPHL 251
           D N+A P + VP+          +A   E     GDDE  P   + R  +       P  
Sbjct: 170 DDNIAAPTEEVPIPGTKAAAAKEEADDDEEESEGGDDEEEPKAALTRSSQPSVSVRYPSS 229

Query: 250 FSE-QGRRQWR 221
           FS+ QG  + R
Sbjct: 230 FSDHQGPEEKR 240



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>RT06_DROME (Q9VZD5) Probable mitochondrial 28S ribosomal protein S6 (MRP-S6)|
          Length = 147

 Score = 29.3 bits (64), Expect = 5.2
 Identities = 12/21 (57%), Positives = 16/21 (76%)
 Frame = -1

Query: 298 VVRQLEDHGHRALPHLFSEQG 236
           ++R+LE+ G RALPH  SE G
Sbjct: 35  IIRKLENLGSRALPHKVSEHG 55



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>QUIA_XANCJ (Q9XD78) Probable quinate dehydrogenase [Pyrroloquinoline-quinone]|
           (EC 1.1.99.25)
          Length = 790

 Score = 29.3 bits (64), Expect = 5.2
 Identities = 25/79 (31%), Positives = 37/79 (46%), Gaps = 7/79 (8%)
 Frame = -1

Query: 436 GGDRNLATPDQRVPLVNRAQGQPLLDAPGP------ERRRGAGDDEALPPLRVVRQL-ED 278
           GG R  A  DQ    +NR+ G+P   +PGP          G G ++ L PL+V  ++   
Sbjct: 159 GGSR-FAALDQ----INRSNGRPAAGSPGPTTPGEIANSDGNGAEDQLTPLQVGEKVFLC 213

Query: 277 HGHRALPHLFSEQGRRQWR 221
             H  L  L +  G++ WR
Sbjct: 214 TPHNNLIALDASTGKQLWR 232



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>APBE_CHLMU (Q9PKW2) Thiamine biosynthesis lipoprotein apbE precursor|
          Length = 316

 Score = 29.3 bits (64), Expect = 5.2
 Identities = 10/39 (25%), Positives = 20/39 (51%)
 Frame = +2

Query: 293 YYTQWGQSLVIAGSAPALGSWSVKQGLSLSPVHQGNALV 409
           YY +WG  +  +G  P+  SW++    +   +H  N+ +
Sbjct: 194 YYVEWGGEIKTSGKHPSGRSWAIASSATPEILHLNNSSI 232



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>RHO_STRLI (P52157) Transcription termination factor rho (EC 3.6.1.-)|
           (ATP-dependent helicase rho)
          Length = 707

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 17/46 (36%), Positives = 20/46 (43%), Gaps = 2/46 (4%)
 Frame = -1

Query: 439 AGGDRNLATPDQRVPLVNRA--QGQPLLDAPGPERRRGAGDDEALP 308
           A   + +  P Q  P VN +  Q  P  DAP   RRR A  D   P
Sbjct: 135 AAAQQQIEIPGQPTPKVNASAEQAAPADDAPSERRRRRATSDAGSP 180



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>LEUD1_DEIRA (Q9RTY5) 3-isopropylmalate dehydratase small subunit 1 (EC|
           4.2.1.33) (Isopropylmalate isomerase 1) (Alpha-IPM
           isomerase 1) (IPMI 1)
          Length = 177

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 13/25 (52%), Positives = 15/25 (60%)
 Frame = -1

Query: 88  GDVNDGGRAWGSGTCEPR*PEGLKK 14
           GDV  GGR WG G+     P+ LKK
Sbjct: 50  GDVLIGGRNWGLGSSREYAPQALKK 74


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,526,568
Number of Sequences: 219361
Number of extensions: 1075745
Number of successful extensions: 4468
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 4024
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4464
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 3026354448
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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