ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl38e07
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SYEP_MOUSE (Q8CGC7) Bifunctional aminoacyl-tRNA synthetase [Incl... 81 1e-15
2SYEP_HUMAN (P07814) Bifunctional aminoacyl-tRNA synthetase [Incl... 80 3e-15
3SYEP_DROME (P28668) Bifunctional aminoacyl-tRNA synthetase [Incl... 70 2e-12
4YHI0_YEAST (P38708) Putative prolyl-tRNA synthetase YHR020W (EC ... 55 9e-08
5SYP_MYCLE (Q9Z5I7) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline... 45 7e-05
6SYP_CLOST (Q9L4Q8) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline... 42 8e-04
7SYP_BORBU (O51363) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline... 41 0.001
8SYP_ARCFU (O28664) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline... 36 0.055
9SYP_MYCPN (P75382) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline... 31 1.3
10SYPC_METJA (Q58635) Bifunctional aminoacyl-tRNA synthetase (ProC... 31 1.8

>SYEP_MOUSE (Q8CGC7) Bifunctional aminoacyl-tRNA synthetase [Includes:|
            Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA
            ligase); Prolyl-tRNA synthetase (EC 6.1.1.15)
            (Proline--tRNA ligase)]
          Length = 1512

 Score = 81.3 bits (199), Expect = 1e-15
 Identities = 32/49 (65%), Positives = 40/49 (81%)
 Frame = +2

Query: 314  LGMAYKKDDNFGEWYSEVVVNSEMIEYYDISGCYILRPWAMEIWELLKD 460
            LG+  KK++N  EWYS+V+  SEMIEYYD+SGCYILRPW+  IWE +KD
Sbjct: 1014 LGLEAKKEENLAEWYSQVITKSEMIEYYDVSGCYILRPWSYSIWESIKD 1062



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>SYEP_HUMAN (P07814) Bifunctional aminoacyl-tRNA synthetase [Includes:|
            Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA
            ligase); Prolyl-tRNA synthetase (EC 6.1.1.15)
            (Proline--tRNA ligase)]
          Length = 1440

 Score = 79.7 bits (195), Expect = 3e-15
 Identities = 32/49 (65%), Positives = 40/49 (81%)
 Frame = +2

Query: 314  LGMAYKKDDNFGEWYSEVVVNSEMIEYYDISGCYILRPWAMEIWELLKD 460
            LG+  KK++N  +WYS+V+  SEMIEY+DISGCYILRPWA  IWE +KD
Sbjct: 942  LGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKD 990



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>SYEP_DROME (P28668) Bifunctional aminoacyl-tRNA synthetase [Includes:|
            Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA
            ligase); Prolyl-tRNA synthetase (EC 6.1.1.15)
            (Proline--tRNA ligase)]
          Length = 1714

 Score = 70.5 bits (171), Expect = 2e-12
 Identities = 27/48 (56%), Positives = 36/48 (75%)
 Frame = +2

Query: 314  LGMAYKKDDNFGEWYSEVVVNSEMIEYYDISGCYILRPWAMEIWELLK 457
            LG+   K+DN  +WYS+V+   EMIEYYD+SGCYILR W+  IW+ +K
Sbjct: 1215 LGLEATKEDNLPDWYSQVITKGEMIEYYDVSGCYILRQWSFAIWKAIK 1262



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>YHI0_YEAST (P38708) Putative prolyl-tRNA synthetase YHR020W (EC 6.1.1.15)|
           (Proline--tRNA ligase) (ProRS)
          Length = 688

 Score = 55.1 bits (131), Expect = 9e-08
 Identities = 21/45 (46%), Positives = 31/45 (68%)
 Frame = +2

Query: 314 LGMAYKKDDNFGEWYSEVVVNSEMIEYYDISGCYILRPWAMEIWE 448
           +G+   K  +F  WY +++   EM++YYD+SGCYILRP +  IWE
Sbjct: 185 IGITVDKALDFPGWYQQILTKGEMLDYYDVSGCYILRPPSYAIWE 229



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>SYP_MYCLE (Q9Z5I7) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA|
           ligase) (ProRS)
          Length = 480

 Score = 45.4 bits (106), Expect = 7e-05
 Identities = 17/44 (38%), Positives = 29/44 (65%)
 Frame = +2

Query: 329 KKDDNFGEWYSEVVVNSEMIEYYDISGCYILRPWAMEIWELLKD 460
           +++D+F  WY+EVV  + +I+     G  ++RP+   IWELL+D
Sbjct: 10  RQEDDFSAWYNEVVAKAGLIDRGPAKGTMVIRPYGYRIWELLQD 53



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>SYP_CLOST (Q9L4Q8) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA|
           ligase) (ProRS)
          Length = 481

 Score = 42.0 bits (97), Expect = 8e-04
 Identities = 11/38 (28%), Positives = 27/38 (71%)
 Frame = +2

Query: 335 DDNFGEWYSEVVVNSEMIEYYDISGCYILRPWAMEIWE 448
           D++F +WY++V+  +++++Y  + G  +++P+   IWE
Sbjct: 16  DEDFPQWYTDVITKTDLVDYSPVKGFMVIKPYGYAIWE 53



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>SYP_BORBU (O51363) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA|
           ligase) (ProRS)
          Length = 488

 Score = 41.2 bits (95), Expect = 0.001
 Identities = 11/42 (26%), Positives = 27/42 (64%)
 Frame = +2

Query: 332 KDDNFGEWYSEVVVNSEMIEYYDISGCYILRPWAMEIWELLK 457
           K+D++ +WY ++V  +++ +Y  + GC ++ P+   IW  ++
Sbjct: 8   KEDDYSKWYLDIVQKAKLADYSPVKGCMVIMPYGYSIWSKIQ 49



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>SYP_ARCFU (O28664) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA|
           ligase) (ProRS)
          Length = 575

 Score = 35.8 bits (81), Expect = 0.055
 Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
 Frame = +2

Query: 320 MAYKKDDNFGEWYSEVVVNSEMIEY-YDISGCYILRPWAMEIWELL 454
           M     +NF EWY EV+  +E+++  Y + G Y+  P+  +I +L+
Sbjct: 105 MTLPSKENFSEWYHEVIQTAEIMDVRYPVKGLYVWFPFGFKIRQLV 150



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>SYP_MYCPN (P75382) Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA|
           ligase) (ProRS)
          Length = 483

 Score = 31.2 bits (69), Expect = 1.3
 Identities = 10/38 (26%), Positives = 20/38 (52%)
 Frame = +2

Query: 332 KDDNFGEWYSEVVVNSEMIEYYDISGCYILRPWAMEIW 445
           KD N   WY +++  ++++ Y D+ G     P +  +W
Sbjct: 4   KDQNLTLWYDQLLSKAQLVSYGDVKGTNCFLPNSWNLW 41



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>SYPC_METJA (Q58635) Bifunctional aminoacyl-tRNA synthetase (ProCysRS)|
           (AATS-CysPro) [Includes: Prolyl-tRNA synthetase (EC
           6.1.1.15) (Proline--tRNA ligase); Cysteinyl-tRNA
           synthetase (EC 6.1.1.16) (Cysteine--tRNA ligase)]
          Length = 455

 Score = 30.8 bits (68), Expect = 1.8
 Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
 Frame = +2

Query: 344 FGEWYSEVVVNSEMIEY-YDISGCYILRPWAMEI 442
           F EWYS+++  +E+ +  Y I GC +  P+  +I
Sbjct: 3   FSEWYSDILEKAEIYDVRYPIKGCGVYLPYGFKI 36


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 42,223,424
Number of Sequences: 219361
Number of extensions: 534741
Number of successful extensions: 1694
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1671
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1693
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2968155324
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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