ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl33a03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1M3K4_HUMAN (Q9Y6R4) Mitogen-activated protein kinase kinase kina... 34 0.23
2WIS4_SCHPO (O14299) MAP kinase kinase kinase wis4 (EC 2.7.11.25)... 33 0.52
3FMT_STRAW (Q827P7) Methionyl-tRNA formyltransferase (EC 2.1.2.9) 32 0.67
4M3K4_MOUSE (O08648) Mitogen-activated protein kinase kinase kina... 32 0.67
5YJS1_YEAST (P46987) Hypothetical 69.5 kDa protein in SWE1-ATP12 ... 30 3.3
6YAP1_YEAST (P19880) AP-1-like transcription factor YAP1 (Phenant... 30 4.4
7SPEN_DROME (Q8SX83) Protein split ends 30 4.4
8CUBN_CANFA (Q9TU53) Cubilin precursor 30 4.4
9NCAP_TACV (P18140) Nucleocapsid protein (Nucleoprotein) 29 5.7
10CO4A4_HUMAN (P53420) Collagen alpha-4(IV) chain precursor 29 5.7
11VGLM_NYV (Q83887) Envelope polyprotein precursor [Contains: Glyc... 28 9.7
12AGP1_YEAST (P25376) General amino acid permease AGP1 (Asparagine... 28 9.7
13NQRF_CHLPN (Q9Z723) Probable Na(+)-translocating NADH-quinone re... 28 9.7

>M3K4_HUMAN (Q9Y6R4) Mitogen-activated protein kinase kinase kinase 4 (EC|
            2.7.11.25) (MAPK/ERK kinase kinase 4) (MEK kinase 4)
            (MEKK 4) (MAP three kinase 1)
          Length = 1607

 Score = 33.9 bits (76), Expect = 0.23
 Identities = 16/53 (30%), Positives = 29/53 (54%), Gaps = 1/53 (1%)
 Frame = +1

Query: 274  WDTGCLCLKFVLGGCVPSPWEDFQSSRKVLAVVGICHLPSSPD-LARVAADFL 429
            W  GC+ ++ V G     PW +++ + +++  VG+ H P  P+ L+    DFL
Sbjct: 1528 WSLGCVVIEMVTG---KRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFL 1577



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>WIS4_SCHPO (O14299) MAP kinase kinase kinase wis4 (EC 2.7.11.25) (MAP kinase|
            kinase kinase wak1) (MAP kinase kinase kinase wik1)
          Length = 1401

 Score = 32.7 bits (73), Expect = 0.52
 Identities = 15/56 (26%), Positives = 28/56 (50%), Gaps = 3/56 (5%)
 Frame = +1

Query: 274  WDTGCLCLKFVLGGCVPSPWEDFQSSRKVLAVVGICHLPSSPD---LARVAADFLD 432
            W  GC+ L+ + G    +PW +  +   ++  V   H PS P    ++ +A DF++
Sbjct: 1232 WSLGCVILEMMTGS---TPWSEMDNEWAIMYHVAAMHTPSIPQNEKISSLARDFIE 1284



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>FMT_STRAW (Q827P7) Methionyl-tRNA formyltransferase (EC 2.1.2.9)|
          Length = 310

 Score = 32.3 bits (72), Expect = 0.67
 Identities = 18/51 (35%), Positives = 26/51 (50%), Gaps = 2/51 (3%)
 Frame = +1

Query: 262 AHQPWDTGCLCLKFVLGGCVPSP--WEDFQSSRKVLAVVGICHLPSSPDLA 408
           AH  W T  L +  V+ GC P+P  W  F+  R  L ++ +  +P   DLA
Sbjct: 210 AHVDWSTPALRVDRVVRGCTPAPGAWTVFRGER--LKLIQVVPVPERTDLA 258



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>M3K4_MOUSE (O08648) Mitogen-activated protein kinase kinase kinase 4 (EC|
            2.7.11.25) (MAPK/ERK kinase kinase 4) (MEK kinase 4)
            (MEKK 4)
          Length = 1597

 Score = 32.3 bits (72), Expect = 0.67
 Identities = 12/43 (27%), Positives = 24/43 (55%)
 Frame = +1

Query: 274  WDTGCLCLKFVLGGCVPSPWEDFQSSRKVLAVVGICHLPSSPD 402
            W  GC+ ++ V G     PW +++ + +++  VG+ H P  P+
Sbjct: 1518 WSLGCVVIEMVTG---KRPWHEYEHNFQIMYKVGMGHKPPIPE 1557



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>YJS1_YEAST (P46987) Hypothetical 69.5 kDa protein in SWE1-ATP12 intergenic|
           region
          Length = 611

 Score = 30.0 bits (66), Expect = 3.3
 Identities = 13/28 (46%), Positives = 19/28 (67%)
 Frame = +3

Query: 18  SPPPRHTTCSVVRSRALPLPLRNGARAE 101
           SPPP +T CS+ RS+ L +PL +  R +
Sbjct: 352 SPPPSNTGCSLHRSKPLHIPLSSVIRED 379



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>YAP1_YEAST (P19880) AP-1-like transcription factor YAP1 (Phenanthroline|
           resistance protein PAR1) (Pleiotropic drug resistance
           protein PDR4)
          Length = 650

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 16/30 (53%), Positives = 20/30 (66%)
 Frame = -3

Query: 137 STATRMADPDSMLSPGSIAEREGESSRSDD 48
           STA R  D   ++SPGS+AE EG  SR D+
Sbjct: 4   STAKRSLD---VVSPGSLAEFEGSKSRHDE 30



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>SPEN_DROME (Q8SX83) Protein split ends|
          Length = 5560

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 24/93 (25%), Positives = 40/93 (43%), Gaps = 2/93 (2%)
 Frame = +1

Query: 94   GLSIESGSAIRVAVLPVGGPIPPQCLRDYAALVARHARVDLASLRPYYSEHQK--SPFAH 267
            G    S +A+  A  P  GPI PQ      +L+ +   + +    P+++  Q   SP +H
Sbjct: 3575 GSPTASQNAMPQASTPKQGPITPQQAIRTQSLIMQPPTISIPEQTPHFAVPQMVLSPQSH 3634

Query: 268  QPWDTGCLCLKFVLGGCVPSPWEDFQSSRKVLA 366
             P   G     +++G   PSP     S  + +A
Sbjct: 3635 HPQQPG----TYMVGIRAPSPHSPLHSPGRGVA 3663



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>CUBN_CANFA (Q9TU53) Cubilin precursor|
          Length = 3620

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 18/46 (39%), Positives = 21/46 (45%)
 Frame = +1

Query: 286 CLCLKFVLGGCVPSPWEDFQSSRKVLAVVGICHLPSSPDLARVAAD 423
           CLC     G    S ++D +   K L V GIC      DL RV AD
Sbjct: 196 CLCSPETHGPQCASKYDDCEGGSKALCVHGICE-----DLVRVKAD 236



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>NCAP_TACV (P18140) Nucleocapsid protein (Nucleoprotein)|
          Length = 570

 Score = 29.3 bits (64), Expect = 5.7
 Identities = 19/57 (33%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
 Frame = +1

Query: 127 VAVLPVGGPIPPQCLRDYAALVARHARVDLASLRPYYSEHQKSPFAHQPWDT-GCLC 294
           + +LP    I  Q   D   L+  H R DL  +   ++  Q   F HQ WD  G LC
Sbjct: 447 IGLLPPNMVITTQGSDDIRKLLDIHGRKDLKLIDVKFTSDQARLFEHQVWDKFGHLC 503



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>CO4A4_HUMAN (P53420) Collagen alpha-4(IV) chain precursor|
          Length = 1690

 Score = 29.3 bits (64), Expect = 5.7
 Identities = 11/20 (55%), Positives = 12/20 (60%)
 Frame = -2

Query: 144 HRQHRHPDGRPGLDAQPGLH 85
           H+  R PDG PG   QPG H
Sbjct: 567 HKGERGPDGPPGFPGQPGSH 586



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>VGLM_NYV (Q83887) Envelope polyprotein precursor [Contains: Glycoprotein 1|
           (G1); Glycoprotein 2 (G2)]
          Length = 1140

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 13/20 (65%), Positives = 14/20 (70%)
 Frame = -3

Query: 137 STATRMADPDSMLSPGSIAE 78
           ST  R ADPD + SPG IAE
Sbjct: 330 STLVRKADPDYVFSPGIIAE 349



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>AGP1_YEAST (P25376) General amino acid permease AGP1 (Asparagine/glutamine|
           permease)
          Length = 595

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 16/42 (38%), Positives = 21/42 (50%)
 Frame = -3

Query: 152 GPPTGSTATRMADPDSMLSPGSIAEREGESSRSDDGAGRVSG 27
           GP     AT+M D  S +SP S   +E E + S D  G  +G
Sbjct: 69  GPQDEVEATQMNDLTSAISPSSRQAQELEKNESSDNIGANTG 110



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>NQRF_CHLPN (Q9Z723) Probable Na(+)-translocating NADH-quinone reductase|
           subunit F (EC 1.6.5.-) (Na(+)-translocating NQR subunit
           F) (Na(+)-NQR subunit F) (NQR complex subunit F) (NQR-1
           subunit F)
          Length = 431

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 19/70 (27%), Positives = 31/70 (44%), Gaps = 5/70 (7%)
 Frame = +1

Query: 100 SIESGSAIRVAVLPVGGPIPPQC-----LRDYAALVARHARVDLASLRPYYSEHQKSPFA 264
           ++ESG  + V++L  G PIP  C      +     V ++A   L + R  +S+ Q     
Sbjct: 54  TVESGQTLLVSLLSSGIPIPSPCGGKATCKQCKVRVVKNADEPLETDRSTFSKRQ----L 109

Query: 265 HQPWDTGCLC 294
            + W   C C
Sbjct: 110 EEGWRLSCQC 119


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,327,978
Number of Sequences: 219361
Number of extensions: 791690
Number of successful extensions: 3383
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 3146
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3383
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3304846491
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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