ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl32a11
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1IF3A_MAIZE (Q9XHR2) Eukaryotic translation initiation factor 3 s... 216 2e-56
2IF3A_TOBAC (Q40554) Eukaryotic translation initiation factor 3 s... 197 2e-50
3IF3A_ARATH (Q9LD55) Eukaryotic translation initiation factor 3 s... 195 5e-50
4IF3A_MOUSE (P23116) Eukaryotic translation initiation factor 3 s... 129 4e-30
5IF3A_HUMAN (Q14152) Eukaryotic translation initiation factor 3 s... 128 7e-30
6IF3A_CAEEL (P34339) Probable eukaryotic translation initiation f... 115 6e-26
7IF3A_SCHPO (O74760) Probable eukaryotic translation initiation f... 100 2e-21
8IF3A_YEAST (P38249) Eukaryotic translation initiation factor 3 1... 87 2e-17
9BPAEB_HUMAN (Q8WXK8) Bullous pemphigoid antigen 1, isoform 7 (Bu... 32 0.93
10Y1002_RHIME (Q52969) Hypothetical sensor-like histidine kinase R... 30 4.6
11CHD1_MOUSE (P40201) Chromodomain-helicase-DNA-binding protein 1 ... 30 4.6
12RAD50_PYRAB (Q9UZC8) DNA double-strand break repair rad50 ATPase 29 6.0
13BRE1B_MACFA (Q4R7K7) Ubiquitin-protein ligase BRE1B (EC 6.3.2.-)... 29 6.0
14ASPM_BOVIN (P62285) Abnormal spindle-like microcephaly-associate... 29 6.0
15BRE1A_CHICK (Q5ZLS3) Ubiquitin-protein ligase BRE1A (EC 6.3.2.-)... 29 7.8
16TGA1A_TOBAC (P14232) TGACG-sequence-specific DNA-binding protein... 29 7.8
17CHD1_HUMAN (O14646) Chromodomain-helicase-DNA-binding protein 1 ... 29 7.8
18BRE1A_HUMAN (Q5VTR2) Ubiquitin-protein ligase BRE1A (EC 6.3.2.-)... 29 7.8

>IF3A_MAIZE (Q9XHR2) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (Eukaryotic translation initiation factor
           3 large subunit) (eIF3a)
          Length = 962

 Score =  216 bits (551), Expect = 2e-56
 Identities = 108/119 (90%), Positives = 115/119 (96%)
 Frame = +1

Query: 121 MATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL 300
           MATFAKPENALKRAEELIHVGQKQ+ALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL
Sbjct: 1   MATFAKPENALKRAEELIHVGQKQSALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL 60

Query: 301 RKGRFAKDGLIQYRIVCQQANVSSLEEVIXHFMQLSNEKAEEARNQAQALEDALDVKIL 477
           RKGRFAKDGLIQYRIVCQQ NVSSLE+VI HFMQLSNEKAE+A++Q +ALEDALDV+ L
Sbjct: 61  RKGRFAKDGLIQYRIVCQQVNVSSLEDVIKHFMQLSNEKAEQAKSQVEALEDALDVEDL 119



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>IF3A_TOBAC (Q40554) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (Eukaryotic translation initiation factor
           3 large subunit) (eIF3a) (PNLA-35)
          Length = 958

 Score =  197 bits (500), Expect = 2e-50
 Identities = 98/119 (82%), Positives = 109/119 (91%)
 Frame = +1

Query: 121 MATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL 300
           MATFAKPENALKRAEELI VGQKQ ALQALHDLITS+RYR+WQK LE+IM KYVELCVD+
Sbjct: 1   MATFAKPENALKRAEELITVGQKQEALQALHDLITSRRYRAWQKTLERIMFKYVELCVDM 60

Query: 301 RKGRFAKDGLIQYRIVCQQANVSSLEEVIXHFMQLSNEKAEEARNQAQALEDALDVKIL 477
           R+GRFAKDGLIQYRIVCQQ N++SLEEVI HFM L+ E+AE ARNQAQALE+ALDV+ L
Sbjct: 61  RRGRFAKDGLIQYRIVCQQVNINSLEEVIKHFMHLATERAELARNQAQALEEALDVEDL 119



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>IF3A_ARATH (Q9LD55) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (Eukaryotic translation initiation factor
           3 large subunit) (eIF3a) (p114)
          Length = 987

 Score =  195 bits (496), Expect = 5e-50
 Identities = 97/123 (78%), Positives = 110/123 (89%)
 Frame = +1

Query: 121 MATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL 300
           MA FAKPENALKRA+ELI+VGQKQ ALQALHDLITSKRYR+WQKPLEKIM KY++LCVDL
Sbjct: 1   MANFAKPENALKRADELINVGQKQDALQALHDLITSKRYRAWQKPLEKIMFKYLDLCVDL 60

Query: 301 RKGRFAKDGLIQYRIVCQQANVSSLEEVIXHFMQLSNEKAEEARNQAQALEDALDVKILS 480
           ++GRFAKDGLIQYRIVCQQ NVSSLEEVI HF+ L+ +KAE+AR+QA ALE+ALDV  L 
Sbjct: 61  KRGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFLHLATDKAEQARSQADALEEALDVDDLE 120

Query: 481 *QR 489
             R
Sbjct: 121 ADR 123



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>IF3A_MOUSE (P23116) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (eIF3 p167) (eIF3 p180) (eIF3 p185)
           (eIF3a) (p162 protein) (Centrosomin)
          Length = 1344

 Score =  129 bits (324), Expect = 4e-30
 Identities = 61/118 (51%), Positives = 88/118 (74%)
 Frame = +1

Query: 124 ATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLR 303
           A F +PENALKRA E + VG+KQ AL  L+D++ SK++R+WQK  E IM+KY+ELCVDLR
Sbjct: 3   AYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLR 62

Query: 304 KGRFAKDGLIQYRIVCQQANVSSLEEVIXHFMQLSNEKAEEARNQAQALEDALDVKIL 477
           K   AK+GL QY+ +CQQ N+ SLE+V+  +++L+ EK E A+ ++Q +   LD++ L
Sbjct: 63  KSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKLAEEKTEAAKEESQQM--VLDIEDL 118



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>IF3A_HUMAN (Q14152) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (eIF3 p167) (eIF3 p180) (eIF3 p185)
           (eIF3a)
          Length = 1382

 Score =  128 bits (322), Expect = 7e-30
 Identities = 60/118 (50%), Positives = 88/118 (74%)
 Frame = +1

Query: 124 ATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLR 303
           A F +PENALKRA E + VG+KQ AL  L+D++ SK++R+WQK  E IM+KY+ELCVDLR
Sbjct: 3   AYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLR 62

Query: 304 KGRFAKDGLIQYRIVCQQANVSSLEEVIXHFMQLSNEKAEEARNQAQALEDALDVKIL 477
           K   AK+GL QY+ +CQQ N+ SLE+V+  +++++ EK E A+ ++Q +   LD++ L
Sbjct: 63  KSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQM--VLDIEDL 118



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>IF3A_CAEEL (P34339) Probable eukaryotic translation initiation factor 3|
           subunit 10 (eIF-3 theta) (eIF3a) (Egg-laying defective
           protein 45)
          Length = 1076

 Score =  115 bits (288), Expect = 6e-26
 Identities = 54/104 (51%), Positives = 76/104 (73%)
 Frame = +1

Query: 130 FAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLRKG 309
           F KPE ALKRAEELI VG++  AL  LHD I ++R++ W    E+IM+K++ELCVDL+K 
Sbjct: 6   FQKPEAALKRAEELIQVGKESDALDTLHDTIKARRHKQWTTVHEQIMIKHMELCVDLKKQ 65

Query: 310 RFAKDGLIQYRIVCQQANVSSLEEVIXHFMQLSNEKAEEARNQA 441
             AKD L QY+ + QQ NV SLE V+ HF++L+ ++ E+A+ Q+
Sbjct: 66  HLAKDALFQYKALTQQINVKSLETVVVHFLKLAEQRTEDAQKQS 109



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>IF3A_SCHPO (O74760) Probable eukaryotic translation initiation factor 3 110|
           kDa subunit (eIF3 p110) (Translation initiation factor
           eIF3, p110 subunit) (eIF3a)
          Length = 932

 Score =  100 bits (250), Expect = 2e-21
 Identities = 49/106 (46%), Positives = 76/106 (71%), Gaps = 1/106 (0%)
 Frame = +1

Query: 136 KPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQK-PLEKIMMKYVELCVDLRKGR 312
           KPEN L+ A+ELI + Q  +ALQ+LH+ I  KR R+ Q   LE IMM+++ELCV LRKG+
Sbjct: 7   KPENVLRLADELIALDQHSSALQSLHETIVLKRSRNAQGFSLEPIMMRFIELCVHLRKGK 66

Query: 313 FAKDGLIQYRIVCQQANVSSLEEVIXHFMQLSNEKAEEARNQAQAL 450
            AK+GL  Y+   Q  +V+++E V+ HF++L+N++ +EA+ +A  +
Sbjct: 67  IAKEGLYTYKNAVQNTSVTAIENVVKHFIELANKRVQEAQEKADKI 112



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>IF3A_YEAST (P38249) Eukaryotic translation initiation factor 3 110 kDa subunit|
           (eIF3 p110) (Translation initiation factor eIF3, p110
           subunit) (eIF3a)
          Length = 964

 Score = 87.0 bits (214), Expect = 2e-17
 Identities = 48/110 (43%), Positives = 73/110 (66%), Gaps = 4/110 (3%)
 Frame = +1

Query: 136 KPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKP--LEKIMMKYVELCVDLRKG 309
           +PENA+KRA+ELI VG+KQAALQ+LHD IT++R R W  P  +E ++ K++E+ V+L+KG
Sbjct: 7   RPENAIKRADELISVGEKQAALQSLHDFITARRIR-WATPSTVEPVVFKFLEIGVELKKG 65

Query: 310 RFAKDGLIQYRIVCQQA--NVSSLEEVIXHFMQLSNEKAEEARNQAQALE 453
           +  KDGL QY+ + Q +   + S+  V   F+ L   K    + +A  L+
Sbjct: 66  KLLKDGLHQYKKLIQGSTEGLVSVGAVARKFIDLVESKIASEQTRADELQ 115



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>BPAEB_HUMAN (Q8WXK8) Bullous pemphigoid antigen 1, isoform 7 (Bullous pemphigoid|
            antigen) (BPA) (Hemidesmosomal plaque protein) (Dystonia
            musculorum protein) (Dystonin) (Fragment)
          Length = 3060

 Score = 32.0 bits (71), Expect = 0.93
 Identities = 20/66 (30%), Positives = 32/66 (48%)
 Frame = +1

Query: 190  QAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLRKGRFAKDGLIQYRIVCQQANVS 369
            QA L+ L D  T  R    Q  +  ++   +  C DL+  +    GLI  +I+CQ   +S
Sbjct: 1245 QAVLRGLIDYDTGIRLLETQLMISGLISPELRKCFDLKDAK--SHGLIDEQILCQLKELS 1302

Query: 370  SLEEVI 387
              +E+I
Sbjct: 1303 KAKEII 1308



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>Y1002_RHIME (Q52969) Hypothetical sensor-like histidine kinase R01002 (EC|
           2.7.13.3)
          Length = 525

 Score = 29.6 bits (65), Expect = 4.6
 Identities = 28/99 (28%), Positives = 42/99 (42%), Gaps = 1/99 (1%)
 Frame = +1

Query: 166 ELIHVGQKQAALQALHDL-ITSKRYRSWQKPLEKIMMKYVELCVDLRKGRFAKDGLIQYR 342
           E IHV  + A L+A+  L + S+R                   VD+R  R + DG     
Sbjct: 149 EQIHVSDRIAFLRAIDSLRLDSERS-----------------AVDIRLERTSADGPQFAH 191

Query: 343 IVCQQANVSSLEEVIXHFMQLSNEKAEEARNQAQALEDA 459
           I C+   +   E  +   +  S + +EEAR QA+A   A
Sbjct: 192 IYCEMTPLRDAEGNLLAIVAQSRDVSEEARLQAEAAAKA 230



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>CHD1_MOUSE (P40201) Chromodomain-helicase-DNA-binding protein 1 (EC 3.6.1.-)|
            (ATP-dependent helicase CHD1) (CHD-1)
          Length = 1711

 Score = 29.6 bits (65), Expect = 4.6
 Identities = 28/125 (22%), Positives = 51/125 (40%), Gaps = 6/125 (4%)
 Frame = +1

Query: 103  RPLAPAMATFAKPENALKRAEELIHVGQKQAAL-----QALHDLITSKRYRSWQKPLEKI 267
            RP+  A+    +PE  L   E+L H  Q    +     + L +    ++ + W+K L   
Sbjct: 1422 RPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYSNPEQIKQWRKNLWIF 1481

Query: 268  MMKYVELCVDLRKGRFAKDGLIQYRIVCQQANVSSLEEVIXHFMQLSN-EKAEEARNQAQ 444
            + K+ E   D RK        I+ R   QQ +  +      H ++  + E+ +E  N   
Sbjct: 1482 VSKFTEF--DARKLHKLYKHAIKKRQESQQNSDQNSNVATTHVIRNPDMERLKENTNHDD 1539

Query: 445  ALEDA 459
            +  D+
Sbjct: 1540 SSRDS 1544



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>RAD50_PYRAB (Q9UZC8) DNA double-strand break repair rad50 ATPase|
          Length = 880

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 25/103 (24%), Positives = 45/103 (43%), Gaps = 6/103 (5%)
 Frame = +1

Query: 133 AKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLRKGR 312
           A  +   K+  E+  +  K   ++        ++ R   K LE I  K  EL + + K +
Sbjct: 201 ANEDELTKKLSEINEISSKLPPIRG-----ELEKVRENVKELESIKGKISELKIQVEKLK 255

Query: 313 FAKDGL------IQYRIVCQQANVSSLEEVIXHFMQLSNEKAE 423
             K GL      I+  I  ++A +S LEE++    +L  ++ E
Sbjct: 256 GRKKGLEEKIVQIERSIEEKKAKISELEEIVKDIPKLQEKEKE 298



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>BRE1B_MACFA (Q4R7K7) Ubiquitin-protein ligase BRE1B (EC 6.3.2.-) (BRE1-B) (RING|
           finger protein 40)
          Length = 1001

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 15/37 (40%), Positives = 20/37 (54%)
 Frame = +1

Query: 364 VSSLEEVIXHFMQLSNEKAEEARNQAQALEDALDVKI 474
           +SS EE+    +Q  N+K  E   Q QA ED L  +I
Sbjct: 41  ISSTEEIDLKVLQFKNKKLAERLEQRQACEDELRERI 77



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>ASPM_BOVIN (P62285) Abnormal spindle-like microcephaly-associated protein|
            homolog (Fragment)
          Length = 3371

 Score = 29.3 bits (64), Expect = 6.0
 Identities = 29/94 (30%), Positives = 44/94 (46%), Gaps = 3/94 (3%)
 Frame = +1

Query: 187  KQAALQALHDLITSKRYRSWQKPLEKIMMKYVEL---CVDLRKGRFAKDGLIQYRIVCQQ 357
            +QA LQ     +T +RY   +K  +   MKYVEL    V L+     +  L++ RI  Q+
Sbjct: 2961 RQAFLQQKSAALTIQRYIRARKAGKHQRMKYVELKKSTVVLQ--ALVRGWLVRKRISEQR 3018

Query: 358  ANVSSLEEVIXHFMQLSNEKAEEARNQAQALEDA 459
            A +  L      F  LS  + + A  +  AL+ A
Sbjct: 3019 AKIRLLHFAAAAFYHLSALRIQRAYRRHVALKHA 3052



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>BRE1A_CHICK (Q5ZLS3) Ubiquitin-protein ligase BRE1A (EC 6.3.2.-) (BRE1-A) (RING|
           finger protein 20)
          Length = 984

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = +1

Query: 364 VSSLEEVIXHFMQLSNEKAEEARNQAQALEDAL 462
           VSS EE+    +Q  N K  E  +Q QA+ED L
Sbjct: 40  VSSTEELDIRTLQTKNRKLAEMLDQRQAIEDEL 72



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>TGA1A_TOBAC (P14232) TGACG-sequence-specific DNA-binding protein TGA-1A (TGA1a)|
           (ASF-1 protein)
          Length = 359

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 26/87 (29%), Positives = 35/87 (40%), Gaps = 1/87 (1%)
 Frame = +1

Query: 223 TSKRYR-SWQKPLEKIMMKYVELCVDLRKGRFAKDGLIQYRIVCQQANVSSLEEVIXHFM 399
           TS RY     KP+EK++ +  +     RK R  K           +A V  LE      +
Sbjct: 60  TSNRYEPETSKPVEKVLRRLAQNREAARKSRLRK-----------KAYVQQLENSKLKLI 108

Query: 400 QLSNEKAEEARNQAQALEDALDVKILS 480
           QL  E  E AR Q   +   +D   LS
Sbjct: 109 QLEQE-LERARKQGMCVGGGVDASQLS 134



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>CHD1_HUMAN (O14646) Chromodomain-helicase-DNA-binding protein 1 (EC 3.6.1.-)|
            (ATP-dependent helicase CHD1) (CHD-1)
          Length = 1709

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 5/73 (6%)
 Frame = +1

Query: 103  RPLAPAMATFAKPENALKRAEELIHVGQKQAAL-----QALHDLITSKRYRSWQKPLEKI 267
            RP+  A+    +PE  L   E+L H  Q    +     + L +    ++ + W+K L   
Sbjct: 1422 RPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIF 1481

Query: 268  MMKYVELCVDLRK 306
            + K+ E   D RK
Sbjct: 1482 VSKFTEF--DARK 1492



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>BRE1A_HUMAN (Q5VTR2) Ubiquitin-protein ligase BRE1A (EC 6.3.2.-) (BRE1-A) (RING|
           finger protein 20)
          Length = 975

 Score = 28.9 bits (63), Expect = 7.8
 Identities = 15/33 (45%), Positives = 19/33 (57%)
 Frame = +1

Query: 364 VSSLEEVIXHFMQLSNEKAEEARNQAQALEDAL 462
           VSS EE+    +Q  N K  E  +Q QA+ED L
Sbjct: 40  VSSTEELDIRTLQTKNRKLAEMLDQRQAIEDEL 72


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,019,165
Number of Sequences: 219361
Number of extensions: 948816
Number of successful extensions: 2708
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 2639
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2706
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3478785780
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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