>TRM1_ARATH (Q9LFU5) Probable N(2),N(2)-dimethylguanosine tRNA|
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 586
Score = 67.8 bits (164), Expect = 2e-11
Identities = 37/85 (43%), Positives = 51/85 (60%), Gaps = 6/85 (7%)
Frame = +1
Query: 271 VREGQAEIFADKSNSVFFNKAQVNNRDLSIAVLRWFISXRREEHDIQLKGR---HAEIPQ 441
++EG+AE+ K N VFFNKAQVNNRD+SIAVLR FI R++EH+ L R ++ +
Sbjct: 11 IKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSKRARSSGKVVE 70
Query: 442 EQHAEYHRSSMPM---D*TGSTGSE 507
+ +E + P D G T E
Sbjct: 71 KDVSETSKEETPTENGDDNGKTNGE 95
>TRM2_ARATH (Q9SRU7) Probable N(2),N(2)-dimethylguanosine tRNA|
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 599
Score = 67.4 bits (163), Expect = 2e-11
Identities = 31/51 (60%), Positives = 40/51 (78%)
Frame = +1
Query: 271 VREGQAEIFADKSNSVFFNKAQVNNRDLSIAVLRWFISXRREEHDIQLKGR 423
++EG+AEI K N VFFNKAQVNNRD+SIAVLR F+S R++EH+ + R
Sbjct: 11 IKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSKR 61
>TRM1_MOUSE (Q3TX08) N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC|
2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
(tRNA 2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 663
Score = 39.3 bits (90), Expect = 0.006
Identities = 24/63 (38%), Positives = 33/63 (52%)
Frame = +1
Query: 268 TVREGQAEIFADKSNSVFFNKAQVNNRDLSIAVLRWFISXRREEHDIQLKGRHAEIPQEQ 447
TV EG A+I +N VF+N Q NRDL+ AV+ F IQ+K +P E+
Sbjct: 57 TVTEGAAKIVFPSANEVFYNPVQEFNRDLTCAVITEFARIHLGAKGIQIK-----VPGEK 111
Query: 448 HAE 456
+E
Sbjct: 112 DSE 114
>TRM1_HUMAN (Q9NXH9) N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC|
2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
(tRNA 2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 659
Score = 38.1 bits (87), Expect = 0.014
Identities = 21/50 (42%), Positives = 28/50 (56%)
Frame = +1
Query: 268 TVREGQAEIFADKSNSVFFNKAQVNNRDLSIAVLRWFISXRREEHDIQLK 417
TV EG A+I +N VF+N Q NRDL+ AV+ F + IQ+K
Sbjct: 56 TVTEGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIK 105
>TRM1_SCHPO (Q9P804) N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC|
2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
(tRNA 2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 548
Score = 37.4 bits (85), Expect = 0.025
Identities = 25/71 (35%), Positives = 37/71 (52%), Gaps = 6/71 (8%)
Frame = +1
Query: 250 TGASPETVREGQAEIFADKSNSVFFNKAQVNNRDLSIAVLRWFISXR------REEHDIQ 411
+GAS ++ EG A I N VF+N Q NRDLS+ +R + R + H Q
Sbjct: 26 SGASA-SLTEGSAIIPFSNPNEVFYNPVQQFNRDLSVTAIRAWSETRSKKIVAKSHHRHQ 84
Query: 412 LKGRHAEIPQE 444
L +++E+ QE
Sbjct: 85 LLDQNSELSQE 95
>TRM1_YEAST (P15565) N(2),N(2)-dimethylguanosine tRNA methyltransferase,|
mitochondrial precursor (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 570
Score = 36.6 bits (83), Expect = 0.042
Identities = 18/43 (41%), Positives = 27/43 (62%)
Frame = +1
Query: 271 VREGQAEIFADKSNSVFFNKAQVNNRDLSIAVLRWFISXRREE 399
V+EG+AEI K +VF+N Q NRDLS+ ++ + + EE
Sbjct: 45 VKEGKAEILFPKKETVFYNPIQQFNRDLSVTCIKAWDNLYGEE 87
>TRM1_CAEEL (Q23270) N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC|
2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
(tRNA 2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 526
Score = 32.3 bits (72), Expect = 0.79
Identities = 20/51 (39%), Positives = 30/51 (58%), Gaps = 2/51 (3%)
Frame = +1
Query: 271 VREGQAEIFADKSNSVFFNKAQVNNRDLSIAVLRWFISXRRE--EHDIQLK 417
++EGQA++ VF+N Q NRDL++ VLR F + ++ E QLK
Sbjct: 24 IQEGQAKV--GFHGPVFYNPVQEFNRDLTVTVLRQFSADHQKWAEEQKQLK 72
>TRM1_DROME (Q9VK89) Probable N(2),N(2)-dimethylguanosine tRNA|
methyltransferase (EC 2.1.1.32)
(tRNA(guanine-26,N(2)-N(2)) methyltransferase) (tRNA
2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 578
Score = 31.6 bits (70), Expect = 1.3
Identities = 20/60 (33%), Positives = 33/60 (55%), Gaps = 1/60 (1%)
Frame = +1
Query: 271 VREGQAEIFADKSNSVFFNKAQVNNRDLSIAVLRWFIS-XRREEHDIQLKGRHAEIPQEQ 447
+RE AEI + +VF+N Q NRDLSIA L + +E + LK + ++ +++
Sbjct: 20 IRERNAEIVS--GGNVFYNPVQEFNRDLSIAALNVYRQRLTKERSEKALKKQRKKVKEQE 77
>TRM1_METMP (Q9HH73) N(2),N(2)-dimethylguanosine tRNA methyltransferase (EC|
2.1.1.32) (tRNA(guanine-26,N(2)-N(2)) methyltransferase)
(tRNA 2,2-dimethylguanosine-26 methyltransferase)
(tRNA(m(2,2)G26)dimethyltransferase)
Length = 373
Score = 28.9 bits (63), Expect = 8.7
Identities = 14/50 (28%), Positives = 29/50 (58%), Gaps = 7/50 (14%)
Frame = +1
Query: 271 VREGQAEIFA------DKSNSVFFNKAQVNNRDLSIAVLRWFI-SXRREE 399
+ EG+ ++ K ++VF+N NRD+S++V++ F+ +R+E
Sbjct: 4 ISEGETKLMVPEESTLSKKDTVFYNPVMETNRDISVSVVQSFLDDFKRDE 53
Database: uniprot_sprot.fasta
Posted date: May 25, 2006 5:36 PM
Number of letters in database: 80,573,946
Number of sequences in database: 219,361
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,731,229
Number of Sequences: 219361
Number of extensions: 1093079
Number of successful extensions: 3352
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 3245
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3352
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3869946934
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)