ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl28e12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (E... 163 2e-40
2EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (E... 160 1e-39
3MANBA_CAPHI (Q95327) Beta-mannosidase precursor (EC 3.2.1.25) (L... 32 0.76
4MANBA_BOVIN (Q29444) Beta-mannosidase precursor (EC 3.2.1.25) (L... 31 1.3
5POLN_SLDV (Q8QL53) Nonstructural polyprotein (Polyprotein nsP123... 29 4.9
6POLN_SPDV (Q8JJX1) Nonstructural polyprotein (Polyprotein nsP123... 29 4.9
7HN_PI4HA (P21526) Hemagglutinin-neuraminidase (EC 3.2.1.18) 28 8.3

>EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
           (Endo-beta-mannosidase) [Contains: Mannosylglycoprotein
           endo-beta-mannosidase 31 kDa subunit;
           Mannosylglycoprotein endo-beta-mannosidase 28 kDa
           subunit; Mannosylglycoprotein end
          Length = 952

 Score =  163 bits (412), Expect = 2e-40
 Identities = 76/103 (73%), Positives = 87/103 (84%)
 Frame = +1

Query: 103 GKLVLDTGWLAARSTEVSLTGVDLTTTHPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDP 282
           GK VLD+GWLAARSTE+ LTGV LTTT PP  + +APW+ A VPGTVLGTLLKNKL+PDP
Sbjct: 1   GKQVLDSGWLAARSTELELTGVQLTTTRPPSGT-SAPWIEAVVPGTVLGTLLKNKLVPDP 59

Query: 283 FYGLNNEAIIDIADSGRDYYTFWFFTTFQCAPAAYQHVSLNFR 411
           FYGLNNE I+DI DSGR+YYTFWFF +F+C  +  QHVSLNFR
Sbjct: 60  FYGLNNEGILDIYDSGREYYTFWFFKSFECKLSENQHVSLNFR 102



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>EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
           (Endo-beta-mannosidase) (AtEBM) [Contains:
           Mannosylglycoprotein endo-beta-mannosidase 31 kDa
           subunit; Mannosylglycoprotein endo-beta-mannosidase 28
           kDa subunit; Mannosylglycopro
          Length = 943

 Score =  160 bits (404), Expect = 1e-39
 Identities = 76/104 (73%), Positives = 83/104 (79%)
 Frame = +1

Query: 100 VGKLVLDTGWLAARSTEVSLTGVDLTTTHPPDASVAAPWMHAAVPGTVLGTLLKNKLIPD 279
           +GK VLD GW+AARSTEV + GV LTTT+PP  S  + WM AAVPGTVLGTL+KNK IPD
Sbjct: 3   IGKTVLDFGWIAARSTEVDVNGVQLTTTNPPAISSESRWMEAAVPGTVLGTLVKNKAIPD 62

Query: 280 PFYGLNNEAIIDIADSGRDYYTFWFFTTFQCAPAAYQHVSLNFR 411
           PFYGL NEAI DIADSGRDYYTFWFFT FQC     Q+V LNFR
Sbjct: 63  PFYGLENEAITDIADSGRDYYTFWFFTKFQCQRLLNQYVHLNFR 106



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>MANBA_CAPHI (Q95327) Beta-mannosidase precursor (EC 3.2.1.25) (Lysosomal beta A|
           mannosidase) (Mannanase) (Mannase)
          Length = 879

 Score = 31.6 bits (70), Expect = 0.76
 Identities = 16/39 (41%), Positives = 21/39 (53%)
 Frame = +1

Query: 184 HPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDPFYGLNN 300
           H  + S+  P   AAVPG V   L   ++I DP+Y  NN
Sbjct: 32  HNGNGSLQLP---AAVPGCVHSALFNKRIIKDPYYRFNN 67



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>MANBA_BOVIN (Q29444) Beta-mannosidase precursor (EC 3.2.1.25) (Lysosomal beta A|
           mannosidase) (Mannanase) (Mannase)
          Length = 879

 Score = 30.8 bits (68), Expect = 1.3
 Identities = 22/77 (28%), Positives = 33/77 (42%)
 Frame = +1

Query: 184 HPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDPFYGLNNEAIIDIADSGRDYYTFWFFTT 363
           H  + S+  P   A VPG V   L   ++I DP+Y  NN   +D      D +T+     
Sbjct: 32  HSGNGSLQLP---ATVPGCVHSALFNKRIIKDPYYRFNN---LDYRWIALDNWTYIKKFK 85

Query: 364 FQCAPAAYQHVSLNFRG 414
                + +  V+L F G
Sbjct: 86  LHSDMSTWSKVNLVFEG 102



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>POLN_SLDV (Q8QL53) Nonstructural polyprotein (Polyprotein nsP1234) (P1234)|
            [Contains: P123; mRNA capping enzyme nsP1 (EC 2.1.1.-)
            (EC 2.7.7.-) (Nonstructural protein 1);
            Protease/triphosphatase/NTPase/helicase nsP2 (EC
            3.4.22.-) (EC 3.1.3.33) (EC 3.6.1.1
          Length = 2593

 Score = 28.9 bits (63), Expect = 4.9
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = -3

Query: 230  TAACIHGAATEASGGWVVVRSTPVRDTSVERA 135
            T+AC HG    A GG  +V++  + D   ERA
Sbjct: 1306 TSACKHGVERTAKGGVFIVKAYGMADRRTERA 1337



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>POLN_SPDV (Q8JJX1) Nonstructural polyprotein (Polyprotein nsP1234) (P1234)|
            [Contains: P123; mRNA capping enzyme nsP1 (EC 2.1.1.-)
            (EC 2.7.7.-) (Nonstructural protein 1);
            Protease/triphosphatase/NTPase/helicase nsP2 (EC
            3.4.22.-) (EC 3.1.3.33) (EC 3.6.1.1
          Length = 2601

 Score = 28.9 bits (63), Expect = 4.9
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = -3

Query: 230  TAACIHGAATEASGGWVVVRSTPVRDTSVERA 135
            T+AC HG    A GG  +V++  + D   ERA
Sbjct: 1307 TSACKHGVERTAKGGVFIVKAYGMADRRTERA 1338



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>HN_PI4HA (P21526) Hemagglutinin-neuraminidase (EC 3.2.1.18)|
          Length = 573

 Score = 28.1 bits (61), Expect = 8.3
 Identities = 22/71 (30%), Positives = 33/71 (46%)
 Frame = +1

Query: 184 HPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDPFYGLNNEAIIDIADSGRDYYTFWFFTT 363
           +PP +S    W+H A PG   G L    LI   + GL  +++I    SG+  Y F   T 
Sbjct: 288 NPPGSS--RDWVHIA-PGVGSGLLYAKLLIFPLYGGLTEKSVIHNNQSGK--YFFPNSTK 342

Query: 364 FQCAPAAYQHV 396
            QC  +  + +
Sbjct: 343 LQCRNSTMEKI 353


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,683,346
Number of Sequences: 219361
Number of extensions: 585149
Number of successful extensions: 1723
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1690
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1722
length of database: 80,573,946
effective HSP length: 100
effective length of database: 58,637,846
effective search space used: 2169600302
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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