ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl28e02
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane pro... 244 8e-65
2AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane pro... 181 9e-46
3AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane pr... 181 1e-45
4HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.... 38 0.015
5HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.... 38 0.015
6HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6... 33 0.37
7HPPA_BRAJA (Q89K83) Pyrophosphate-energized proton pump (EC 3.6.... 33 0.49
8HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.... 32 0.64
9PRDM1_HUMAN (O75626) PR domain zinc finger protein 1 (PR domain-... 30 3.2
10RYR1_RABIT (P11716) Ryanodine receptor 1 (Skeletal muscle-type r... 30 4.1
11HPPA_RHORT (O68460) Pyrophosphate-energized proton pump (EC 3.6.... 29 5.4
12TRUB_MYCLE (Q9Z5I4) tRNA pseudouridine synthase B (EC 5.4.99.-) ... 29 5.4
13HPPA1_RHOPA (P60363) Pyrophosphate-energized proton pump (EC 3.6... 29 5.4
14ACOD_PIG (O02858) Acyl-CoA desaturase (EC 1.14.19.1) (Stearoyl-C... 29 7.1
15RYR1_PIG (P16960) Ryanodine receptor 1 (Skeletal muscle-type rya... 29 7.1
16RYR1_HUMAN (P21817) Ryanodine receptor 1 (Skeletal muscle-type r... 28 9.2
17HPPA1_METMA (Q8PYZ8) Pyrophosphate-energized proton pump 1 (EC 3... 28 9.2
18HPPA1_METAC (Q8TJA9) Pyrophosphate-energized proton pump 1 (EC 3... 28 9.2

>AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase)
          Length = 762

 Score =  244 bits (623), Expect = 8e-65
 Identities = 122/125 (97%), Positives = 123/125 (98%)
 Frame = +2

Query: 101 MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAATGAKNGYGDYLIEEEE 280
           MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAA GAKNGYGDYLIEEEE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAAAGAKNGYGDYLIEEEE 60

Query: 281 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTXGQP 460
           GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFST GQP
Sbjct: 61  GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 120

Query: 461 CTYSR 475
           CTYS+
Sbjct: 121 CTYSK 125



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>AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase) (Vacuolar
           H(+)-pyrophosphatase)
          Length = 764

 Score =  181 bits (459), Expect = 9e-46
 Identities = 91/125 (72%), Positives = 103/125 (82%), Gaps = 1/125 (0%)
 Frame = +2

Query: 104 AILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGA-ASAATGAKNGYGDYLIEEEE 280
           AIL +LGTEILIPVC VIGI FA+ QW +VSKVK++    AS    AKNGY DYLIEEEE
Sbjct: 3   AILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPNAAAKNGYNDYLIEEEE 62

Query: 281 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTXGQP 460
           G+NDHNVVVKCAEIQ AISEGATSFLFT Y+YVG+FMV FA +IFLFLGS+EGFST  Q 
Sbjct: 63  GINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFSTSPQA 122

Query: 461 CTYSR 475
           C+Y +
Sbjct: 123 CSYDK 127



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>AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane proton pump 1|
           (EC 3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase 1) (H(+)-PPase 1) (Vacuolar proton
           pyrophosphatase 1) (Vacuolar proton pyrophosphatase 3)
          Length = 770

 Score =  181 bits (458), Expect = 1e-45
 Identities = 88/130 (67%), Positives = 109/130 (83%), Gaps = 4/130 (3%)
 Frame = +2

Query: 92  LTAMAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTP--GAASA--ATGAKNGYGD 259
           + A A+L EL TEIL+P+C VIGI F++ QW++VS+VK+T   GA+S+  A   KNGYGD
Sbjct: 1   MVAPALLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGD 60

Query: 260 YLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEG 439
           YLIEEEEG+ND +VV KCAEIQTAISEGATSFLFT Y+YVG+FM+ FAA+IF+FLGS+EG
Sbjct: 61  YLIEEEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEG 120

Query: 440 FSTXGQPCTY 469
           FST  +PCTY
Sbjct: 121 FSTDNKPCTY 130



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>HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 37.7 bits (86), Expect = 0.015
 Identities = 31/99 (31%), Positives = 46/99 (46%), Gaps = 2/99 (2%)
 Frame = +2

Query: 155 IGIVFAVAQWFIVSKVKVTPGAASAATGAKNGYGDYLIEEEEGLNDHNVVVKCAEIQTAI 334
           + I+ A++   IV+ V  T    S   G   G       E+E         K  EI +AI
Sbjct: 4   VTIIIAMSILAIVTAVVYTLKVTSIKVGTLGG------NEKE-------TKKLLEISSAI 50

Query: 335 SEGATSFLFTMYQYVGMFMVVFAAIIFLFLGS--IEGFS 445
           SEGA +FL   Y+ + +F+   A +I L L +   EGF+
Sbjct: 51  SEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN 89



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>HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 37.7 bits (86), Expect = 0.015
 Identities = 31/99 (31%), Positives = 46/99 (46%), Gaps = 2/99 (2%)
 Frame = +2

Query: 155 IGIVFAVAQWFIVSKVKVTPGAASAATGAKNGYGDYLIEEEEGLNDHNVVVKCAEIQTAI 334
           + I+ A++   IV+ V  T    S   G   G       E+E         K  EI +AI
Sbjct: 4   VTIIIAMSILAIVTAVVYTLKVTSIKVGTLGG------NEKE-------TKKLLEISSAI 50

Query: 335 SEGATSFLFTMYQYVGMFMVVFAAIIFLFLGS--IEGFS 445
           SEGA +FL   Y+ + +F+   A +I L L +   EGF+
Sbjct: 51  SEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN 89



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>HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 725

 Score = 33.1 bits (74), Expect = 0.37
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = +2

Query: 317 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSI 433
           EI  AI +GA ++L   Y+ + +   V A +IF FLG +
Sbjct: 40  EIAGAIQQGAAAYLARQYKTIAIVGAVLAILIFFFLGGL 78



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>HPPA_BRAJA (Q89K83) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 706

 Score = 32.7 bits (73), Expect = 0.49
 Identities = 25/98 (25%), Positives = 40/98 (40%)
 Frame = +2

Query: 134 LIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAATGAKNGYGDYLIEEEEGLNDHNVVVKC 313
           LI +CGV+ +V+A+  W        T    SA  G+                      + 
Sbjct: 6   LIVLCGVLSVVYAI--W-------ATSSVLSADAGSP---------------------RM 35

Query: 314 AEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 427
            EI  A+ EGA ++L   Y  +G+  +V   ++  FLG
Sbjct: 36  QEIAAAVREGAQAYLRRQYTTIGIVGIVIFVLLVYFLG 73



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>HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 673

 Score = 32.3 bits (72), Expect = 0.64
 Identities = 15/37 (40%), Positives = 24/37 (64%)
 Frame = +2

Query: 317 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 427
           EI   I +GA +FL T Y+Y+  F+V+   I+ +F+G
Sbjct: 38  EIAGHIHDGAMAFLKTEYKYLTGFIVIVTVILAIFVG 74



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>PRDM1_HUMAN (O75626) PR domain zinc finger protein 1 (PR domain-containing|
           protein 1) (Beta-interferon gene positive-regulatory
           domain I-binding factor) (BLIMP-1) (Positive regulatory
           domain I-binding factor 1) (PRDI-binding factor 1)
           (PRDI-BF1)
          Length = 789

 Score = 30.0 bits (66), Expect = 3.2
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = -2

Query: 456 WPXVLNPSIDPRKRKIIAAKTTMNIPTYWYMVKRNDVAPSEMAVW 322
           W   +NP+  PR++ + A +  MNI  Y+Y +K    A  E+ VW
Sbjct: 122 WMRYVNPAHSPREQNLAACQNGMNI--YFYTIKPIP-ANQELLVW 163



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>RYR1_RABIT (P11716) Ryanodine receptor 1 (Skeletal muscle-type ryanodine|
            receptor) (RyR1) (RYR-1) (Skeletal muscle calcium release
            channel)
          Length = 5037

 Score = 29.6 bits (65), Expect = 4.1
 Identities = 18/59 (30%), Positives = 26/59 (44%)
 Frame = +2

Query: 221  ASAATGAKNGYGDYLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVV 397
            A A +G  +G+G    EE EG  D N+V    E  T   E A   L  ++  V    ++
Sbjct: 4601 AGAGSGGGSGWGSGAGEEAEGDEDENMVYYFLEESTGYMEPALWCLSLLHTLVAFLCII 4659



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>HPPA_RHORT (O68460) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 702

 Score = 29.3 bits (64), Expect = 5.4
 Identities = 22/72 (30%), Positives = 32/72 (44%)
 Frame = +2

Query: 227 AATGAKNGYGDYLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAA 406
           AA  A  GYG   I+     +      +  EI  A+ EGA++FL   Y+ + +   V   
Sbjct: 10  AAALAALGYGALTIKTIMAADAGTA--RMQEISGAVQEGASAFLNRQYKTIAVVGAVVFV 67

Query: 407 IIFLFLGSIEGF 442
           I+   LG   GF
Sbjct: 68  ILTALLGISVGF 79



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>TRUB_MYCLE (Q9Z5I4) tRNA pseudouridine synthase B (EC 5.4.99.-) (tRNA|
           pseudouridine 55 synthase) (Psi55 synthase)
           (tRNA-uridine isomerase) (tRNA pseudouridylate synthase)
          Length = 320

 Score = 29.3 bits (64), Expect = 5.4
 Identities = 12/34 (35%), Positives = 21/34 (61%)
 Frame = +2

Query: 221 ASAATGAKNGYGDYLIEEEEGLNDHNVVVKCAEI 322
           + ++TGA  G G  +I++  G+  H+VV +C  I
Sbjct: 2   SESSTGAGLGPGIVVIDKPSGMTSHDVVARCRRI 35



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>HPPA1_RHOPA (P60363) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 706

 Score = 29.3 bits (64), Expect = 5.4
 Identities = 25/98 (25%), Positives = 39/98 (39%)
 Frame = +2

Query: 134 LIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAATGAKNGYGDYLIEEEEGLNDHNVVVKC 313
           +I +CG + IV+A+  W           A S+   A  G                   + 
Sbjct: 6   VIVLCGALSIVYAI--W-----------ATSSVLAADQGNA-----------------RM 35

Query: 314 AEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 427
            EI  A+ EGA ++L   Y  + +  VV  A++  FLG
Sbjct: 36  QEIAGAVREGAQAYLKRQYMTIAIVGVVIFALLAYFLG 73



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>ACOD_PIG (O02858) Acyl-CoA desaturase (EC 1.14.19.1) (Stearoyl-CoA|
           desaturase) (Fatty acid desaturase)
           (Delta(9)-desaturase) (Fragment)
          Length = 334

 Score = 28.9 bits (63), Expect = 7.1
 Identities = 17/43 (39%), Positives = 27/43 (62%)
 Frame = +2

Query: 92  LTAMAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGA 220
           L ++  LG L   ILIP C +  +++A A ++++S V VT GA
Sbjct: 67  LMSLLHLGALYGIILIPTCKIYTLLWAFA-YYLLSAVGVTAGA 108



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>RYR1_PIG (P16960) Ryanodine receptor 1 (Skeletal muscle-type ryanodine|
            receptor) (RyR1) (RYR-1) (Skeletal muscle calcium release
            channel)
          Length = 5035

 Score = 28.9 bits (63), Expect = 7.1
 Identities = 17/59 (28%), Positives = 26/59 (44%)
 Frame = +2

Query: 221  ASAATGAKNGYGDYLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVV 397
            + A +G  +G+G    EE EG  D N+V    E  T   E A   L  ++  V    ++
Sbjct: 4599 SGAGSGGGSGWGSGAGEEVEGDEDENMVYYFLEESTGYMEPALRCLSLLHTLVAFLCII 4657



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>RYR1_HUMAN (P21817) Ryanodine receptor 1 (Skeletal muscle-type ryanodine|
            receptor) (RyR1) (RYR-1) (Skeletal muscle calcium release
            channel)
          Length = 5038

 Score = 28.5 bits (62), Expect = 9.2
 Identities = 17/59 (28%), Positives = 26/59 (44%)
 Frame = +2

Query: 221  ASAATGAKNGYGDYLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVV 397
            + A +G  +G+G    EE EG  D N+V    E  T   E A   L  ++  V    ++
Sbjct: 4602 SGAGSGGSSGWGLGAGEEAEGDEDENMVYYFLEESTGYMEPALRCLSLLHTLVAFLCII 4660



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>HPPA1_METMA (Q8PYZ8) Pyrophosphate-energized proton pump 1 (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase 1)
           (H+-PPase 1) (Membrane-bound proton-translocating
           pyrophosphatase 1)
          Length = 676

 Score = 28.5 bits (62), Expect = 9.2
 Identities = 24/97 (24%), Positives = 38/97 (39%)
 Frame = +2

Query: 134 LIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAATGAKNGYGDYLIEEEEGLNDHNVVVKC 313
           L P+C +IG++FA                 S       G G+ LI+              
Sbjct: 7   LTPICALIGLIFA---------------GISYKNVRNEGEGNELIK-------------- 37

Query: 314 AEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFL 424
            +I  AI  GA  +L   Y+ + +F+V  A ++ L L
Sbjct: 38  -KITAAIHGGAMVYLNRQYRAIAVFVVFIAIVLALVL 73



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>HPPA1_METAC (Q8TJA9) Pyrophosphate-energized proton pump 1 (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase 1)
           (H+-PPase 1) (Membrane-bound proton-translocating
           pyrophosphatase 1)
          Length = 676

 Score = 28.5 bits (62), Expect = 9.2
 Identities = 25/97 (25%), Positives = 38/97 (39%)
 Frame = +2

Query: 134 LIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAATGAKNGYGDYLIEEEEGLNDHNVVVKC 313
           L P+C +IG++FA                 S       G G+ LI+              
Sbjct: 7   LAPICALIGLIFA---------------GISYKNVQNEGAGNDLIK-------------- 37

Query: 314 AEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFL 424
            +I  +I  GA  +L   Y+ + +F+V  A II L L
Sbjct: 38  -KITASIHGGAMVYLNRQYRAIAVFVVFLAIIIALIL 73


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,723,456
Number of Sequences: 219361
Number of extensions: 585315
Number of successful extensions: 2443
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 2384
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2437
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3130907202
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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