ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl28a10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1VPS35_MOUSE (Q9EQH3) Vacuolar protein sorting 35 (Vesicle protei... 112 7e-25
2VPS35_HUMAN (Q96QK1) Vacuolar protein sorting 35 (Vesicle protei... 112 7e-25
3VPS35_YEAST (P34110) Vacuolar protein sorting-associated protein... 81 1e-15
4VPS35_SCHPO (O74552) Vacuolar protein sorting-associated protein... 62 1e-09
5LAP2_HUMAN (Q96RT1) LAP2 protein (Erbb2-interacting protein) (Er... 33 0.56
6CUT7_SCHPO (P24339) Kinesin-like protein cut7 (Cell untimely tor... 32 0.73
7LAP2_MOUSE (Q80TH2) LAP2 protein (Erbb2-interacting protein) (Er... 32 0.73
8SEC71_SCHPO (Q9UT02) Protein transport protein sec71 30 4.7
9NEMO_MOUSE (O88522) NF-kappa-B essential modulator (NEMO) (NF-ka... 29 8.1

>VPS35_MOUSE (Q9EQH3) Vacuolar protein sorting 35 (Vesicle protein sorting 35)|
           (Maternal-embryonic 3)
          Length = 796

 Score =  112 bits (279), Expect = 7e-25
 Identities = 57/106 (53%), Positives = 74/106 (69%)
 Frame = +2

Query: 182 DDEERWLAEGIAGVQQNAFYMHRALDSNNLKDALKYSAQMLSELRTSRLSPHKYYELYMR 361
           D++E+ L E I  V+  +F M R LD N L DALK+++ ML ELRTS LSP  YYELYM 
Sbjct: 10  DEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMA 69

Query: 362 AFDEMRKLEMFFREETRRGSCSVVDLYELVQHAGHVLPRLYLLCTV 499
             DE+  LE++  +E  +G   V DLYELVQ+AG+++PRLYLL TV
Sbjct: 70  ISDELHYLEVYLTDEFAKGR-KVADLYELVQYAGNIIPRLYLLITV 114



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>VPS35_HUMAN (Q96QK1) Vacuolar protein sorting 35 (Vesicle protein sorting 35)|
           (hVPS35) (Maternal-embryonic 3)
          Length = 796

 Score =  112 bits (279), Expect = 7e-25
 Identities = 57/106 (53%), Positives = 74/106 (69%)
 Frame = +2

Query: 182 DDEERWLAEGIAGVQQNAFYMHRALDSNNLKDALKYSAQMLSELRTSRLSPHKYYELYMR 361
           D++E+ L E I  V+  +F M R LD N L DALK+++ ML ELRTS LSP  YYELYM 
Sbjct: 10  DEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMA 69

Query: 362 AFDEMRKLEMFFREETRRGSCSVVDLYELVQHAGHVLPRLYLLCTV 499
             DE+  LE++  +E  +G   V DLYELVQ+AG+++PRLYLL TV
Sbjct: 70  ISDELHYLEVYLTDEFAKGR-KVADLYELVQYAGNIIPRLYLLITV 114



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>VPS35_YEAST (P34110) Vacuolar protein sorting-associated protein VPS35|
          Length = 944

 Score = 81.3 bits (199), Expect = 1e-15
 Identities = 42/96 (43%), Positives = 62/96 (64%)
 Frame = +2

Query: 212 IAGVQQNAFYMHRALDSNNLKDALKYSAQMLSELRTSRLSPHKYYELYMRAFDEMRKLEM 391
           IA ++Q    M+R L  + L ++L++++ ML+ELR   LSP KYYELY+  FD +  L  
Sbjct: 11  IAVIKQRTALMNRCLSQHKLMESLQHTSIMLTELRNPNLSPKKYYELYVIIFDSLTNLST 70

Query: 392 FFREETRRGSCSVVDLYELVQHAGHVLPRLYLLCTV 499
           +  E   +    + DLYELVQ+ G+V+PRLYL+ TV
Sbjct: 71  YLIENHPQNH-HLADLYELVQYTGNVVPRLYLMITV 105



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>VPS35_SCHPO (O74552) Vacuolar protein sorting-associated protein vps35|
          Length = 785

 Score = 61.6 bits (148), Expect = 1e-09
 Identities = 30/63 (47%), Positives = 43/63 (68%)
 Frame = +2

Query: 311 LRTSRLSPHKYYELYMRAFDEMRKLEMFFREETRRGSCSVVDLYELVQHAGHVLPRLYLL 490
           +R S L+P +YYELYM   + +R L     E    G+ +++DLYELVQ+AG ++PRLYL+
Sbjct: 1   MRNSALTPKQYYELYMFNMESLRLLGGTLLETHLNGTHNLMDLYELVQYAGSIVPRLYLM 60

Query: 491 CTV 499
            TV
Sbjct: 61  ITV 63



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>LAP2_HUMAN (Q96RT1) LAP2 protein (Erbb2-interacting protein) (Erbin)|
           (Densin-180-like protein)
          Length = 1412

 Score = 32.7 bits (73), Expect = 0.56
 Identities = 13/28 (46%), Positives = 20/28 (71%)
 Frame = -3

Query: 435 STTEQLPRRVSSLKNISSFRISSNALMY 352
           ++ +QLP  + SLKNI++ +I  N LMY
Sbjct: 263 NSLQQLPETIGSLKNITTLKIDENQLMY 290



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>CUT7_SCHPO (P24339) Kinesin-like protein cut7 (Cell untimely torn protein 7)|
          Length = 1085

 Score = 32.3 bits (72), Expect = 0.73
 Identities = 26/104 (25%), Positives = 48/104 (46%), Gaps = 7/104 (6%)
 Frame = +2

Query: 173 GGGDDEERWLAEGIAGVQQNAFYMHRALDSNNLKDALKYSAQMLSELRTSRLSPHKYYEL 352
           G   D +  L+EG   + +  + +  +LD++N + A+K S                YYEL
Sbjct: 171 GDLSDSDGILSEGAGLIPRALYQLFSSLDNSNQEYAVKCS----------------YYEL 214

Query: 353 Y------MRAFDEMRKLEMFFREETRRGSCSVVDLYE-LVQHAG 463
           Y      +   +E+RK    F + +RRG+  +  + E  +++AG
Sbjct: 215 YNEEIRDLLVSEELRKPARVFEDTSRRGNVVITGIEESYIKNAG 258



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>LAP2_MOUSE (Q80TH2) LAP2 protein (Erbb2-interacting protein) (Erbin)|
           (Densin-180-like protein)
          Length = 1402

 Score = 32.3 bits (72), Expect = 0.73
 Identities = 12/28 (42%), Positives = 20/28 (71%)
 Frame = -3

Query: 435 STTEQLPRRVSSLKNISSFRISSNALMY 352
           ++ +QLP  + SLKN+++ +I  N LMY
Sbjct: 263 NSLQQLPETIGSLKNVTTLKIDENQLMY 290



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>SEC71_SCHPO (Q9UT02) Protein transport protein sec71|
          Length = 1811

 Score = 29.6 bits (65), Expect = 4.7
 Identities = 30/113 (26%), Positives = 49/113 (43%), Gaps = 11/113 (9%)
 Frame = +2

Query: 170  DGGGDDEERWLAEGIAGVQQNAFYMHRALD-SNNLKDA-----LKYSAQMLSELRTSRLS 331
            D G +  + +L E    +Q+N   +    D ++N  +      L ++A + + L T    
Sbjct: 860  DDGANLSDSFLTEVYEEIQKNEIVLKDEQDPTSNFPEIPGTSNLSFAANISNALATVGRD 919

Query: 332  PHKYYELYMRAFDEM-RKLEMFF----REETRRGSCSVVDLYELVQHAGHVLP 475
              +  E Y  A ++M  K E  F    RE+  RG  S  D+Y   +H  HV P
Sbjct: 920  LQR--EAYYMASNKMANKTEALFKDLIREQRERGKLSGNDIYYTARHFEHVCP 970



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>NEMO_MOUSE (O88522) NF-kappa-B essential modulator (NEMO) (NF-kappa-B|
           essential modifier) (Inhibitor of nuclear factor kappa-B
           kinase gamma subunit) (IkB kinase gamma subunit)
           (I-kappa-B kinase gamma) (IKK-gamma) (IKKG) (IkB
           kinase-associated protein 1)
          Length = 412

 Score = 28.9 bits (63), Expect = 8.1
 Identities = 27/96 (28%), Positives = 41/96 (42%), Gaps = 15/96 (15%)
 Frame = +2

Query: 143 NTAAARMLPDGGGDDEERWLAEG----------IAGVQQNAFYMHRALDSNN-LKDALKY 289
           N  +  + P GG  +++  L E           +   Q     + R L+ N  L+DA++ 
Sbjct: 8   NQLSETVQPSGGPAEDQDMLGEESSLGKPAMLHLPSEQGTPETLQRCLEENQELRDAIRQ 67

Query: 290 SAQML----SELRTSRLSPHKYYELYMRAFDEMRKL 385
           S QML     EL   ++S  +  E  M  F E RKL
Sbjct: 68  SNQMLRERCEELLHFQVSQREEKEFLMCKFQEARKL 103


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,916,804
Number of Sequences: 219361
Number of extensions: 667496
Number of successful extensions: 2525
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 2458
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2522
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3581144924
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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