ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl28a05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (E... 207 1e-53
2EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (E... 197 1e-50
3MANBA_CAPHI (Q95327) Beta-mannosidase precursor (EC 3.2.1.25) (L... 34 0.21
4MANBA_BOVIN (Q29444) Beta-mannosidase precursor (EC 3.2.1.25) (L... 33 0.36
5POLN_SLDV (Q8QL53) Nonstructural polyprotein (Polyprotein nsP123... 29 8.9
6POLN_SPDV (Q8JJX1) Nonstructural polyprotein (Polyprotein nsP123... 29 8.9
7ASSY_THET2 (P61526) Argininosuccinate synthase (EC 6.3.4.5) (Cit... 29 8.9

>EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
           (Endo-beta-mannosidase) [Contains: Mannosylglycoprotein
           endo-beta-mannosidase 31 kDa subunit;
           Mannosylglycoprotein endo-beta-mannosidase 28 kDa
           subunit; Mannosylglycoprotein end
          Length = 952

 Score =  207 bits (528), Expect = 1e-53
 Identities = 96/126 (76%), Positives = 109/126 (86%)
 Frame = +2

Query: 101 GKLVLDTGWLAARSTEVSLTGVDLTTTHPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDP 280
           GK VLD+GWLAARSTE+ LTGV LTTT PP  + +APW+ A VPGTVLGTLLKNKL+PDP
Sbjct: 1   GKQVLDSGWLAARSTELELTGVQLTTTRPPSGT-SAPWIEAVVPGTVLGTLLKNKLVPDP 59

Query: 281 FYGLNNEAIIDIADSGRDYYTFWFFTTFQCAPAANQHVSLNFRGVNYSAEVYINGHXEIL 460
           FYGLNNE I+DI DSGR+YYTFWFF +F+C  + NQHVSLNFR +NYSAEVY+NGH EIL
Sbjct: 60  FYGLNNEGILDIYDSGREYYTFWFFKSFECKLSENQHVSLNFRAINYSAEVYLNGHKEIL 119

Query: 461 PKGMFR 478
           PKGMFR
Sbjct: 120 PKGMFR 125



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>EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
           (Endo-beta-mannosidase) (AtEBM) [Contains:
           Mannosylglycoprotein endo-beta-mannosidase 31 kDa
           subunit; Mannosylglycoprotein endo-beta-mannosidase 28
           kDa subunit; Mannosylglycopro
          Length = 943

 Score =  197 bits (501), Expect = 1e-50
 Identities = 92/127 (72%), Positives = 103/127 (81%)
 Frame = +2

Query: 98  VGKLVLDTGWLAARSTEVSLTGVDLTTTHPPDASVAAPWMHAAVPGTVLGTLLKNKLIPD 277
           +GK VLD GW+AARSTEV + GV LTTT+PP  S  + WM AAVPGTVLGTL+KNK IPD
Sbjct: 3   IGKTVLDFGWIAARSTEVDVNGVQLTTTNPPAISSESRWMEAAVPGTVLGTLVKNKAIPD 62

Query: 278 PFYGLNNEAIIDIADSGRDYYTFWFFTTFQCAPAANQHVSLNFRGVNYSAEVYINGHXEI 457
           PFYGL NEAI DIADSGRDYYTFWFFT FQC    NQ+V LNFR +NYSA+V++NGH   
Sbjct: 63  PFYGLENEAITDIADSGRDYYTFWFFTKFQCQRLLNQYVHLNFRAINYSAQVFVNGHKTE 122

Query: 458 LPKGMFR 478
           LPKGMFR
Sbjct: 123 LPKGMFR 129



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>MANBA_CAPHI (Q95327) Beta-mannosidase precursor (EC 3.2.1.25) (Lysosomal beta A|
           mannosidase) (Mannanase) (Mannase)
          Length = 879

 Score = 34.3 bits (77), Expect = 0.21
 Identities = 26/87 (29%), Positives = 39/87 (44%)
 Frame = +2

Query: 182 HPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDPFYGLNNEAIIDIADSGRDYYTFWFFTT 361
           H  + S+  P   AAVPG V   L   ++I DP+Y  NN   +D      D +T+     
Sbjct: 32  HNGNGSLQLP---AAVPGCVHSALFNKRIIKDPYYRFNN---LDYRWIALDNWTYIKKFK 85

Query: 362 FQCAPAANQHVSLNFRGVNYSAEVYIN 442
                +    V+L F G++  A V +N
Sbjct: 86  LHSDMSEWNKVNLVFEGIDTVAVVLLN 112



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>MANBA_BOVIN (Q29444) Beta-mannosidase precursor (EC 3.2.1.25) (Lysosomal beta A|
           mannosidase) (Mannanase) (Mannase)
          Length = 879

 Score = 33.5 bits (75), Expect = 0.36
 Identities = 25/87 (28%), Positives = 38/87 (43%)
 Frame = +2

Query: 182 HPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDPFYGLNNEAIIDIADSGRDYYTFWFFTT 361
           H  + S+  P   A VPG V   L   ++I DP+Y  NN   +D      D +T+     
Sbjct: 32  HSGNGSLQLP---ATVPGCVHSALFNKRIIKDPYYRFNN---LDYRWIALDNWTYIKKFK 85

Query: 362 FQCAPAANQHVSLNFRGVNYSAEVYIN 442
                +    V+L F G++  A V +N
Sbjct: 86  LHSDMSTWSKVNLVFEGIDTVAVVLLN 112



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>POLN_SLDV (Q8QL53) Nonstructural polyprotein (Polyprotein nsP1234) (P1234)|
            [Contains: P123; mRNA capping enzyme nsP1 (EC 2.1.1.-)
            (EC 2.7.7.-) (Nonstructural protein 1);
            Protease/triphosphatase/NTPase/helicase nsP2 (EC
            3.4.22.-) (EC 3.1.3.33) (EC 3.6.1.1
          Length = 2593

 Score = 28.9 bits (63), Expect = 8.9
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = -2

Query: 228  TAACIHGAATEASGGWVVVRSTPVRDTSVERA 133
            T+AC HG    A GG  +V++  + D   ERA
Sbjct: 1306 TSACKHGVERTAKGGVFIVKAYGMADRRTERA 1337



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>POLN_SPDV (Q8JJX1) Nonstructural polyprotein (Polyprotein nsP1234) (P1234)|
            [Contains: P123; mRNA capping enzyme nsP1 (EC 2.1.1.-)
            (EC 2.7.7.-) (Nonstructural protein 1);
            Protease/triphosphatase/NTPase/helicase nsP2 (EC
            3.4.22.-) (EC 3.1.3.33) (EC 3.6.1.1
          Length = 2601

 Score = 28.9 bits (63), Expect = 8.9
 Identities = 13/32 (40%), Positives = 18/32 (56%)
 Frame = -2

Query: 228  TAACIHGAATEASGGWVVVRSTPVRDTSVERA 133
            T+AC HG    A GG  +V++  + D   ERA
Sbjct: 1307 TSACKHGVERTAKGGVFIVKAYGMADRRTERA 1338



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>ASSY_THET2 (P61526) Argininosuccinate synthase (EC 6.3.4.5)|
           (Citrulline--aspartate ligase)
          Length = 400

 Score = 28.9 bits (63), Expect = 8.9
 Identities = 17/52 (32%), Positives = 23/52 (44%), Gaps = 4/52 (7%)
 Frame = +2

Query: 335 YYTFWFFTTFQCAPAANQHVSLNFRGVN----YSAEVYINGHXEILPKGMFR 478
           YY FW+    +   A   HV+ N  GV     Y   VY+ G     PK ++R
Sbjct: 310 YYGFWYAPEREALQAYFDHVAKNVTGVARLKLYKGNVYVVGRK--APKSLYR 359


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,004,712
Number of Sequences: 219361
Number of extensions: 853711
Number of successful extensions: 2306
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 2262
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2305
length of database: 80,573,946
effective HSP length: 104
effective length of database: 57,760,402
effective search space used: 3927707336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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