ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl26b11
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1IF3X_DICDI (O15818) Putative eukaryotic translation initiation f... 118 9e-27
2IF3X_HUMAN (O75153) Putative eukaryotic translation initiation f... 115 4e-26
3IF3X_SCHPO (O59742) Probable eukaryotic translation initiation f... 102 5e-22
4IF3X_CAEEL (P34466) Putative eukaryotic translation initiation f... 93 4e-19
5IF3X_YEAST (Q03690) Eukaryotic translation initiation factor 3 1... 43 3e-04
6OAZ_CANGA (Q6FJC4) Ornithine decarboxylase antizyme 32 0.81
7KLF17_HUMAN (Q5JT82) Krueppel-like factor 17 (Zinc finger protei... 28 8.9
8RECF_SYNSC (Q3AML2) DNA replication and repair protein recF 28 8.9
9ATR_ASHGO (Q75DB8) Serine/threonine-protein kinase MEC1 (EC 2.7.... 28 8.9

>IF3X_DICDI (O15818) Putative eukaryotic translation initiation factor 3|
           subunit (eIF-3)
          Length = 1321

 Score =  118 bits (295), Expect = 9e-27
 Identities = 56/145 (38%), Positives = 92/145 (63%)
 Frame = +2

Query: 2   KISAKFKKGFREILDRKASAHPFENVQALLPVTSWLGAHPVPEHRRDSARAEDSVVLSYG 181
           ++S  F++G  +IL      HPF+ +  +LPV +W+ +     +R D  +  D+ V    
Sbjct: 341 QVSRLFRRGLNQILTNIGRNHPFDMLPGVLPVHNWVASSKT--NRYDINKGTDTFVSVQD 398

Query: 182 TELIGMQRDWNEELQSCREFPHANPQERILRGRALYKVTCDFVDAAVKGAVGVINRCIPP 361
            EL G  RDWNEE+Q+ +E P +  QERI+R RA+ KV  +FV+ A++GA  ++++ I P
Sbjct: 399 VELRGNPRDWNEEIQAPKELPKSTVQERIIRDRAISKVNSEFVECAIRGAQVIVDKAILP 458

Query: 362 INPTDPECFHMYVHNNXFFSFAVDS 436
           INP + +  HM+++NN FFS+A+D+
Sbjct: 459 INPAENQRSHMFLYNNIFFSYALDT 483



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>IF3X_HUMAN (O75153) Putative eukaryotic translation initiation factor 3|
           subunit (eIF-3)
          Length = 1309

 Score =  115 bits (289), Expect = 4e-26
 Identities = 62/146 (42%), Positives = 81/146 (55%), Gaps = 2/146 (1%)
 Frame = +2

Query: 2   KISAKFKKGFREILDRKASAHPFENVQALLPVTSWLGAHPVPEHRRDSARAEDSVV--LS 175
           +IS  FKK F  +  ++   HPFE +     V SW    P  EH  D  RAED+    L 
Sbjct: 288 QISPTFKKNFAVLQKKRVQRHPFERIATPFQVYSWTA--PQAEHAMDCVRAEDAYTSRLG 345

Query: 176 YGTELIGMQRDWNEELQSCREFPHANPQERILRGRALYKVTCDFVDAAVKGAVGVINRCI 355
           Y   + G  RDWNEELQ+ RE P  N  ER+LR RA++KV  DF  AA +GA+ VI+  +
Sbjct: 346 YEEHIPGQTRDWNEELQTTRELPRKNLPERLLRERAIFKVHSDFTAAATRGAMAVIDGNV 405

Query: 356 PPINPTDPECFHMYVHNNXFFSFAVD 433
             INP++     M++ NN FFS   D
Sbjct: 406 MAINPSEETKMQMFIWNNIFFSLGFD 431



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>IF3X_SCHPO (O59742) Probable eukaryotic translation initiation factor 3 135|
           kDa subunit (eIF3 p135) (Translation initiation factor
           eIF3, p135 subunit)
          Length = 1173

 Score =  102 bits (254), Expect = 5e-22
 Identities = 54/121 (44%), Positives = 72/121 (59%)
 Frame = +2

Query: 74  NVQALLPVTSWLGAHPVPEHRRDSARAEDSVVLSYGTELIGMQRDWNEELQSCREFPHAN 253
           ++   LP   W+   PVP HR D +R + S +  Y  E  G  RDWNEE+QS RE  H +
Sbjct: 301 SITGTLPQAPWI-TFPVP-HRADLSRTQKSELFPY-IENQGNLRDWNEEIQSTREMDHED 357

Query: 254 PQERILRGRALYKVTCDFVDAAVKGAVGVINRCIPPINPTDPECFHMYVHNNXFFSFAVD 433
            Q+R+LR R   K   DF D AV+GA+ ++N  IP +NP +P    M+VHNN FFS+  D
Sbjct: 358 VQDRVLRERLTVKTLQDFTDMAVEGAIDMVNGNIPSLNPLEPTASQMFVHNNIFFSYGRD 417

Query: 434 S 436
           S
Sbjct: 418 S 418



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>IF3X_CAEEL (P34466) Putative eukaryotic translation initiation factor 3|
           subunit (eIF-3)
          Length = 1247

 Score = 92.8 bits (229), Expect = 4e-19
 Identities = 55/159 (34%), Positives = 88/159 (55%), Gaps = 7/159 (4%)
 Frame = +2

Query: 5   ISAKFKKGFREILDRKASAHPFENVQALLPVTSWLGAHPVPE--HRRDSARA---EDSVV 169
           +S  FKK + +IL R+      E +    PV+SW+G +P+    +  DS RA    +   
Sbjct: 284 VSPGFKKVYPQILKRRQEKTLVERLPTSYPVSSWVG-NPLKTDGYMSDSLRAIELTEPFR 342

Query: 170 LSYGTELIGMQRDWNEELQSCREFPHANPQERILRGRALYKVTCDFVDAAVKGAVGVINR 349
           + +   + G+ RDWNEELQ+  E    +  ER++R R+ YK+  D+V+AA +G   +++ 
Sbjct: 343 VGFEDHMPGLLRDWNEELQTTFEMTRKSISERVIRDRSYYKIHADYVNAAARGVQSILDG 402

Query: 350 CIPPINPTDPECFHMYVHNNXFFS--FAVDSDYEQISKD 460
            I  INP + +  HMY+ NN FFS  F V   Y+++  D
Sbjct: 403 NILAINPGEDKKTHMYIWNNIFFSLGFDVRDHYKELGGD 441



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>IF3X_YEAST (Q03690) Eukaryotic translation initiation factor 3 135 kDa subunit|
           (eIF3 p135) (Translation initiation factor eIF3, p135
           subunit)
          Length = 1277

 Score = 43.1 bits (100), Expect = 3e-04
 Identities = 24/90 (26%), Positives = 45/90 (50%), Gaps = 2/90 (2%)
 Frame = +2

Query: 200 QRDWNEELQSCREFPHANPQERILRGRALYKVTCDFVDAAVKGAVGVINRCIPPINPTDP 379
           +R++N+E Q+ ++   +  Q+RI   R   KV  +F   A  GA+ +       +NP  P
Sbjct: 358 ERNFNDEFQAIKDLTTSTLQDRIEMERLFSKVVHEFSVTAASGAMSIFYSDFVAMNPESP 417

Query: 380 ECFHMYVHNNXFFSFA--VDSDYEQISKDQ 463
               +++ +N F+S+   V  +YE    D+
Sbjct: 418 TRDQIFLKDNIFYSYVSDVSGNYEGKGGDE 447



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>OAZ_CANGA (Q6FJC4) Ornithine decarboxylase antizyme|
          Length = 255

 Score = 32.0 bits (71), Expect = 0.81
 Identities = 14/40 (35%), Positives = 21/40 (52%)
 Frame = +2

Query: 74  NVQALLPVTSWLGAHPVPEHRRDSARAEDSVVLSYGTELI 193
           N+Q LL    W+GA+ +P   R+     D + LS  T +I
Sbjct: 211 NIQTLLKNLHWIGANLLPNENRNECNENDDITLSDETYII 250



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>KLF17_HUMAN (Q5JT82) Krueppel-like factor 17 (Zinc finger protein 393)|
          Length = 389

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 14/52 (26%), Positives = 25/52 (48%)
 Frame = +2

Query: 95  VTSWLGAHPVPEHRRDSARAEDSVVLSYGTELIGMQRDWNEELQSCREFPHA 250
           ++ W  AH   +   +SA   +   +S  +   G+   WN+ L S + FPH+
Sbjct: 16  LSRWQAAHQAAQDNENSAPILN---MSSSSGSSGVHTSWNQGLPSIQHFPHS 64



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>RECF_SYNSC (Q3AML2) DNA replication and repair protein recF|
          Length = 364

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 21/60 (35%), Positives = 27/60 (45%)
 Frame = +2

Query: 98  TSWLGAHPVPEHRRDSARAEDSVVLSYGTELIGMQRDWNEELQSCREFPHANPQERILRG 277
           T  L  H  P  R D   AE+   L+   E +  QR+  E L SCR  PH +    +L G
Sbjct: 209 TETLELHYKPGSRLDGEDAEEPWRLAI-EEQLRQQREEEERLGSCRVGPHRDEIALLLGG 267



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>ATR_ASHGO (Q75DB8) Serine/threonine-protein kinase MEC1 (EC 2.7.11.1)|
            (DNA-damage checkpoint kinase MEC1) (Mitosis entry
            checkpoint protein 1) (ATR homolog)
          Length = 2324

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 29/113 (25%), Positives = 43/113 (38%), Gaps = 6/113 (5%)
 Frame = +2

Query: 20   KKGFREILDRKASAHPFENVQALLPVTSWLGAHPVPEHRRDSARAEDSVVLSYG-TELIG 196
            +KG + + DR       +  + LL  T WL          D +    SV +S+   E+IG
Sbjct: 1706 RKGIKTLKDR-------DRAKVLLKYTEWL----------DLSNNATSVQISHQYKEVIG 1748

Query: 197  MQRDWNEELQS-----CREFPHANPQERILRGRALYKVTCDFVDAAVKGAVGV 340
            + +DW+E   S      R          +  G   YK    F+ A  K  V V
Sbjct: 1749 LDKDWDEPYYSFGLYYSRLLEKKRADGFVTTGSLEYKAITYFLSAFEKNTVKV 1801


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,645,585
Number of Sequences: 219361
Number of extensions: 1345196
Number of successful extensions: 3419
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 3341
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3415
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 3026354448
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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