ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl26b01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1IMB1_SCHPO (O13864) Importin beta-1 subunit (Karyopherin beta-1 ... 61 1e-09
2IMB1_MOUSE (P70168) Importin beta-1 subunit (Karyopherin beta-1 ... 56 6e-08
3IMB1_HUMAN (Q14974) Importin beta-1 subunit (Karyopherin beta-1 ... 56 6e-08
4IMB_DROME (O18388) Importin beta subunit (Karyopherin beta-4 sub... 49 9e-06
5IMB1_YEAST (Q06142) Importin beta-1 subunit (Karyopherin beta-1 ... 49 9e-06
6IMB1_RAT (P52296) Importin beta-1 subunit (Karyopherin beta-1 su... 45 8e-05
7TNPO1_MOUSE (Q8BFY9) Transportin-1 (Importin beta-2) (Karyopheri... 40 0.004
8TNPO1_HUMAN (Q92973) Transportin-1 (Importin beta-2) (Karyopheri... 40 0.004
9TOP2_LEICH (O61078) DNA topoisomerase 2 (EC 5.99.1.3) (DNA topoi... 35 0.082
10TNPO2_HUMAN (O14787) Transportin-2 (Karyopherin beta-2b) 35 0.11
11TNPO2_MOUSE (Q99LG2) Transportin-2 (Karyopherin beta-2b) 35 0.11
12IMB5_YEAST (P53067) Importin beta-5 subunit (Karyopherin beta-5 ... 32 0.70
13PTPRV_RAT (Q64612) Receptor-type tyrosine-protein phosphatase V ... 32 0.91
14K1949_PIG (Q767M0) Protein KIAA1949 homolog 30 4.5
15YPB3_LACLA (P42097) Putative HTH-type transcriptional regulator ... 29 5.9
16KPYK_TRYBO (Q27788) Pyruvate kinase (EC 2.7.1.40) (PK) 29 7.7
17DPO4_THETN (P58965) DNA polymerase IV (EC 2.7.7.7) (Pol IV) 29 7.7

>IMB1_SCHPO (O13864) Importin beta-1 subunit (Karyopherin beta-1 subunit)|
           (Importin 95)
          Length = 863

 Score = 61.2 bits (147), Expect = 1e-09
 Identities = 34/96 (35%), Positives = 51/96 (53%)
 Frame = +3

Query: 177 MDITQILLAAQSPDGNLRSAAEANIKQFQEQNLPNFLLSLSVELSNDERPPESRRLAGII 356
           M+  + L    SPD N+R  AE  ++     +   +++ L+ EL+ND   P  R  AG+ 
Sbjct: 1   MNAGEFLAQTLSPDANVRLNAEKQLENAARTDFAQYMVLLAQELANDNSMPYIRMAAGLA 60

Query: 357 LKNSLDAKDSARKGLLIQQWVSLDHSIKSQIKESLL 464
           LKN++ A++ ARK    Q W SL   IK Q+K   L
Sbjct: 61  LKNAITAREEARKLEYQQLWQSLPVEIKQQVKSLAL 96



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>IMB1_MOUSE (P70168) Importin beta-1 subunit (Karyopherin beta-1 subunit)|
           (Nuclear factor P97) (Pore targeting complex 97 kDa
           subunit) (PTAC97) (SCG)
          Length = 876

 Score = 55.8 bits (133), Expect = 6e-08
 Identities = 32/96 (33%), Positives = 54/96 (56%)
 Frame = +3

Query: 177 MDITQILLAAQSPDGNLRSAAEANIKQFQEQNLPNFLLSLSVELSNDERPPESRRLAGII 356
           M++  IL    SPD     AA+  +++   +NLP FL+ LS  L+N      +R  AG+ 
Sbjct: 1   MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQ 60

Query: 357 LKNSLDAKDSARKGLLIQQWVSLDHSIKSQIKESLL 464
           +KNSL +KD   K    Q+W+++D + + ++K  +L
Sbjct: 61  IKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVL 96



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>IMB1_HUMAN (Q14974) Importin beta-1 subunit (Karyopherin beta-1 subunit)|
           (Nuclear factor P97) (Importin 90)
          Length = 876

 Score = 55.8 bits (133), Expect = 6e-08
 Identities = 32/96 (33%), Positives = 54/96 (56%)
 Frame = +3

Query: 177 MDITQILLAAQSPDGNLRSAAEANIKQFQEQNLPNFLLSLSVELSNDERPPESRRLAGII 356
           M++  IL    SPD     AA+  +++   +NLP FL+ LS  L+N      +R  AG+ 
Sbjct: 1   MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQ 60

Query: 357 LKNSLDAKDSARKGLLIQQWVSLDHSIKSQIKESLL 464
           +KNSL +KD   K    Q+W+++D + + ++K  +L
Sbjct: 61  IKNSLTSKDPDIKAQYQQRWLAIDANARREVKNYVL 96



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>IMB_DROME (O18388) Importin beta subunit (Karyopherin beta-4 subunit)|
           (Protein ketel)
          Length = 884

 Score = 48.5 bits (114), Expect = 9e-06
 Identities = 32/96 (33%), Positives = 45/96 (46%)
 Frame = +3

Query: 177 MDITQILLAAQSPDGNLRSAAEANIKQFQEQNLPNFLLSLSVELSNDERPPESRRLAGII 356
           M +  IL    SPD N   +A+  ++Q    NLP FL +LS  L N      +R  AG+ 
Sbjct: 7   MQLIAILEKTVSPDKNELLSAKNFLEQAAASNLPEFLKALSEILVNTANSAVARMAAGLQ 66

Query: 357 LKNSLDAKDSARKGLLIQQWVSLDHSIKSQIKESLL 464
           LKN L +KD         +W      I+  IK ++L
Sbjct: 67  LKNHLTSKDEKVSQQYQDRWHQFPSEIRELIKNNIL 102



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>IMB1_YEAST (Q06142) Importin beta-1 subunit (Karyopherin beta-1 subunit)|
           (Importin 95)
          Length = 861

 Score = 48.5 bits (114), Expect = 9e-06
 Identities = 29/86 (33%), Positives = 44/86 (51%), Gaps = 1/86 (1%)
 Frame = +3

Query: 210 SPDGNLRSAAEANIKQFQEQNLPNFLLSLSVELSNDERPPESRRLAGIILKNSLDAKDSA 389
           SPD N+R  +E  +K+    N   F    S  L ++    E R LA + LKN L +KDS 
Sbjct: 16  SPDQNIRLTSETQLKKLSNDNFLQFAGLSSQVLIDENTKLEGRILAALTLKNELVSKDSV 75

Query: 390 RKGLLIQQWVS-LDHSIKSQIKESLL 464
           +     Q+W++ +    K+QIK + L
Sbjct: 76  KTQQFAQRWITQVSPEAKNQIKTNAL 101



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>IMB1_RAT (P52296) Importin beta-1 subunit (Karyopherin beta-1 subunit)|
           (Nuclear factor P97)
          Length = 875

 Score = 45.4 bits (106), Expect = 8e-05
 Identities = 29/96 (30%), Positives = 52/96 (54%)
 Frame = +3

Query: 177 MDITQILLAAQSPDGNLRSAAEANIKQFQEQNLPNFLLSLSVELSNDERPPESRRLAGII 356
           M++  IL    SPD     AA+  +++   +NLP FL+ LS  L+N      +R  AG+ 
Sbjct: 1   MELITILEKTVSPDRLELEAAQKFLERAAVENLPTFLVELSRVLANPGNSQVARVAAGLQ 60

Query: 357 LKNSLDAKDSARKGLLIQQWVSLDHSIKSQIKESLL 464
           ++  L +KD   K    Q+W+++D + + ++K  +L
Sbjct: 61  IR-LLTSKDPDIKAQYQQRWLAIDANARREVKNYVL 95



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>TNPO1_MOUSE (Q8BFY9) Transportin-1 (Importin beta-2) (Karyopherin beta-2)|
          Length = 890

 Score = 39.7 bits (91), Expect = 0.004
 Identities = 21/69 (30%), Positives = 42/69 (60%), Gaps = 1/69 (1%)
 Frame = +3

Query: 174 VMDITQILLAAQSPDGNLRSAAEANIKQF-QEQNLPNFLLSLSVELSNDERPPESRRLAG 350
           +  I Q+L  +QSPD  ++   +  ++Q  Q  +  N+L+ +  +L +++ P  +R L+G
Sbjct: 12  LQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEP--TRSLSG 69

Query: 351 IILKNSLDA 377
           +ILKN++ A
Sbjct: 70  LILKNNVKA 78



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>TNPO1_HUMAN (Q92973) Transportin-1 (Importin beta-2) (Karyopherin beta-2) (M9|
           region interaction protein) (MIP)
          Length = 890

 Score = 39.7 bits (91), Expect = 0.004
 Identities = 21/69 (30%), Positives = 42/69 (60%), Gaps = 1/69 (1%)
 Frame = +3

Query: 174 VMDITQILLAAQSPDGNLRSAAEANIKQF-QEQNLPNFLLSLSVELSNDERPPESRRLAG 350
           +  I Q+L  +QSPD  ++   +  ++Q  Q  +  N+L+ +  +L +++ P  +R L+G
Sbjct: 12  LQQILQLLKESQSPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEP--TRSLSG 69

Query: 351 IILKNSLDA 377
           +ILKN++ A
Sbjct: 70  LILKNNVKA 78



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>TOP2_LEICH (O61078) DNA topoisomerase 2 (EC 5.99.1.3) (DNA topoisomerase II)|
          Length = 1236

 Score = 35.4 bits (80), Expect = 0.082
 Identities = 22/70 (31%), Positives = 35/70 (50%), Gaps = 1/70 (1%)
 Frame = +3

Query: 219 GNLRSAAEANIKQFQE-QNLPNFLLSLSVELSNDERPPESRRLAGIILKNSLDAKDSARK 395
           G L +    N+K+ +  + L +  LSL +EL  + R P   R   +++    DA  S  K
Sbjct: 467 GKLLNVRNKNLKRLKTCKGLQDLFLSLGLELGKEYRSPAELRYQRLLVMTDQDADGSHIK 526

Query: 396 GLLIQQWVSL 425
           GL+I  + SL
Sbjct: 527 GLVINAFESL 536



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>TNPO2_HUMAN (O14787) Transportin-2 (Karyopherin beta-2b)|
          Length = 897

 Score = 35.0 bits (79), Expect = 0.11
 Identities = 19/69 (27%), Positives = 40/69 (57%), Gaps = 1/69 (1%)
 Frame = +3

Query: 174 VMDITQILLAAQSPDGNLRSAAEANIKQFQE-QNLPNFLLSLSVELSNDERPPESRRLAG 350
           +  + Q+L  +QSP+   +   +  +KQ  +  +  N+L+ +   L +++ P  +R L+G
Sbjct: 10  LQQVLQLLKDSQSPNTATQRIVQDKLKQLNQFPDFNNYLIFVLTRLKSEDEP--TRSLSG 67

Query: 351 IILKNSLDA 377
           +ILKN++ A
Sbjct: 68  LILKNNVKA 76



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>TNPO2_MOUSE (Q99LG2) Transportin-2 (Karyopherin beta-2b)|
          Length = 887

 Score = 35.0 bits (79), Expect = 0.11
 Identities = 19/69 (27%), Positives = 40/69 (57%), Gaps = 1/69 (1%)
 Frame = +3

Query: 174 VMDITQILLAAQSPDGNLRSAAEANIKQFQE-QNLPNFLLSLSVELSNDERPPESRRLAG 350
           +  + Q+L  +QSP+   +   +  +KQ  +  +  N+L+ +   L +++ P  +R L+G
Sbjct: 10  LQQVLQLLKDSQSPNTATQRIVQDKLKQLNQFPDFNNYLIFVLTRLKSEDEP--TRSLSG 67

Query: 351 IILKNSLDA 377
           +ILKN++ A
Sbjct: 68  LILKNNVKA 76



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>IMB5_YEAST (P53067) Importin beta-5 subunit (Karyopherin beta-5 subunit) (114|
           kDa karyopherin)
          Length = 1004

 Score = 32.3 bits (72), Expect = 0.70
 Identities = 18/62 (29%), Positives = 32/62 (51%)
 Frame = +3

Query: 177 MDITQILLAAQSPDGNLRSAAEANIKQFQEQNLPNFLLSLSVELSNDERPPESRRLAGII 356
           MDI ++++ AQS D + R  AE  + Q+ + +      +L+      E   ESR+ A + 
Sbjct: 1   MDINELIIGAQSADKHTREVAETQLLQWCDSDASQVFKALANVALQHEASLESRQFALLS 60

Query: 357 LK 362
           L+
Sbjct: 61  LR 62



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>PTPRV_RAT (Q64612) Receptor-type tyrosine-protein phosphatase V precursor (EC|
           3.1.3.48) (Embryonic stem cell protein-tyrosine
           phosphatase) (ES cell phosphatase) (Osteotesticular
           protein-tyrosine phosphatase) (OST-PTP)
          Length = 1711

 Score = 32.0 bits (71), Expect = 0.91
 Identities = 19/40 (47%), Positives = 23/40 (57%)
 Frame = -1

Query: 315 HSITPPTRIGGSWEDSAPGTA*CWLLLLSEGYHLETEQLA 196
           HS   P R+  SW D APG    + LLL   YHLE++ LA
Sbjct: 137 HSAGSPARLEASWSD-APGDQDSYQLLL---YHLESQTLA 172



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>K1949_PIG (Q767M0) Protein KIAA1949 homolog|
          Length = 618

 Score = 29.6 bits (65), Expect = 4.5
 Identities = 15/40 (37%), Positives = 20/40 (50%)
 Frame = -2

Query: 215 WRLSS*QNLSDIHDCCTCPEERICRGMLPRSLARLPASQT 96
           WRLSS +   D  + C   EER   GM+P  +   P + T
Sbjct: 267 WRLSSGEERKDCLEECGRKEERTLPGMVPEDITGSPETLT 306



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>YPB3_LACLA (P42097) Putative HTH-type transcriptional regulator in lacX|
           3'region (ORF3)
          Length = 196

 Score = 29.3 bits (64), Expect = 5.9
 Identities = 29/98 (29%), Positives = 47/98 (47%), Gaps = 6/98 (6%)
 Frame = +3

Query: 192 ILLAAQSPDGNLRSAAE------ANIKQFQEQNLPNFLLSLSVELSNDERPPESRRLAGI 353
           IL   +SP  NL+   +      A  K +    + N ++ LS  L  D R P  +  +  
Sbjct: 75  ILSRDESPLANLKEMLDWIYSDHAQKKIYYGCPVGNLVIELSA-LDEDFRKPLEQLFSD- 132

Query: 354 ILKNSLDAKDSARKGLLIQQWVSLDHSIKSQIKESLLI 467
            L+  +    SA  GLL++Q +   H+I +QI+ SLL+
Sbjct: 133 -LQKKIAENLSALTGLLVKQNLPAAHAIIAQIQGSLLL 169



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>KPYK_TRYBO (Q27788) Pyruvate kinase (EC 2.7.1.40) (PK)|
          Length = 498

 Score = 28.9 bits (63), Expect = 7.7
 Identities = 10/32 (31%), Positives = 19/32 (59%)
 Frame = -2

Query: 239 CC*AKVTIWRLSS*QNLSDIHDCCTCPEERIC 144
           CC  +VT+W +++ + + ++      PEE IC
Sbjct: 350 CCETQVTMWNMAAFEAIKNLQSFPLIPEEAIC 381



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>DPO4_THETN (P58965) DNA polymerase IV (EC 2.7.7.7) (Pol IV)|
          Length = 384

 Score = 28.9 bits (63), Expect = 7.7
 Identities = 24/78 (30%), Positives = 34/78 (43%), Gaps = 6/78 (7%)
 Frame = +3

Query: 246 NIKQFQEQNLPNFLLSLSVELSN-----DERPPESRRLAGIILKNSLDAKDSARKGLLIQ 410
           ++ +  ++NL        VE+ N     DERP E+ R    I K     KD+  K LLIQ
Sbjct: 199 DLLKLPQENLIELFGKTGVEIYNRIRGIDERPVETMREIKSIGKEKTLEKDTKNKELLIQ 258

Query: 411 QWVSLDHSIKSQ-IKESL 461
                   +  + IKE L
Sbjct: 259 HLKEFSEIVSEELIKERL 276


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,062,231
Number of Sequences: 219361
Number of extensions: 1050220
Number of successful extensions: 2875
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 2808
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2873
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3420806017
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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