ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl25e09
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SASH1_MOUSE (P59808) SAM and SH3 domain-containing protein 1 37 0.033
2CAN5_MOUSE (O08688) Calpain-5 (EC 3.4.22.-) (nCL-3) 29 5.3
3CWC22_NEUCR (Q7RX84) Pre-mRNA-splicing factor cwc-22 29 6.9
4PLCB_HUMAN (O15120) 1-acyl-sn-glycerol-3-phosphate acyltransfera... 28 9.1
5CAC1G_HUMAN (O43497) Voltage-dependent T-type calcium channel al... 28 9.1
6CAC1G_RAT (O54898) Voltage-dependent T-type calcium channel alph... 28 9.1
7UBP53_HUMAN (Q70EK8) Inactive ubiquitin carboxyl-terminal hydrol... 28 9.1
8MRAZ_PSEU2 (Q4ZNY1) Protein mraZ 28 9.1
9MRAZ_PSE14 (Q48EE9) Protein mraZ 28 9.1

>SASH1_MOUSE (P59808) SAM and SH3 domain-containing protein 1|
          Length = 1230

 Score = 36.6 bits (83), Expect = 0.033
 Identities = 27/91 (29%), Positives = 37/91 (40%)
 Frame = +3

Query: 153 D*CKMVTVHGFANGTSDGTANRKIKRLFNSSERKNPTNFQFERHVARLESRQQQQPRRCI 332
           D  K   +H   N T      R  K+L    E K P+    E HV   E+   Q  R  +
Sbjct: 247 DSVKFKRLHKLVNST-----RRVRKKLIRVEEMKKPSAEGGEEHV--FENSPVQDERSAL 299

Query: 333 FTTILPIEFFHTSSEPTSPEDSLDSASSPPS 425
           ++ +    FF+  S    PED  DS +  PS
Sbjct: 300 YSGVHKKPFFYDGSPEKPPEDDADSLTPSPS 330



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>CAN5_MOUSE (O08688) Calpain-5 (EC 3.4.22.-) (nCL-3)|
          Length = 640

 Score = 29.3 bits (64), Expect = 5.3
 Identities = 15/53 (28%), Positives = 27/53 (50%), Gaps = 12/53 (22%)
 Frame = -3

Query: 439 CKKIIDGGEDAESR------------LSSGDVGSDEVWKNSIGKIVVKIHRLG 317
           C + +DGG  A++             L+ GD+ +DE  +N + + V+K+H  G
Sbjct: 173 CYQALDGGNTADALVDFTGGVSEPIDLTEGDLATDEAKRNQLFERVLKVHSRG 225



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>CWC22_NEUCR (Q7RX84) Pre-mRNA-splicing factor cwc-22|
          Length = 1010

 Score = 28.9 bits (63), Expect = 6.9
 Identities = 13/23 (56%), Positives = 16/23 (69%)
 Frame = -1

Query: 159 ISRWPPRQGGGRSCRSAPLRSQS 91
           +SR PPR+G GRS    P RS+S
Sbjct: 749 LSRTPPRRGRGRSYSRTPSRSRS 771



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>PLCB_HUMAN (O15120) 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (EC|
           2.3.1.51) (1-AGP acyltransferase 2) (1-AGPAT 2)
           (Lysophosphatidic acid acyltransferase-beta)
           (LPAAT-beta) (1-acylglycerol-3-phosphate
           O-acyltransferase 2)
          Length = 278

 Score = 28.5 bits (62), Expect = 9.1
 Identities = 20/63 (31%), Positives = 29/63 (46%), Gaps = 1/63 (1%)
 Frame = -3

Query: 274 NWKLVGFFL-SDELKRRLIFLLAVPSLVPLANPCTVTILHQSVAAAAGGREELPLRSAPI 98
           N  ++G+F+ S +    L F +  P  +  A PC +   HQS+    G  E LP R   I
Sbjct: 59  NMSIIGWFVRSFKYFYGLRFEVRDPRRLQEARPCVIVSNHQSILDMMGLMEVLPERCVQI 118

Query: 97  PVR 89
             R
Sbjct: 119 AKR 121



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>CAC1G_HUMAN (O43497) Voltage-dependent T-type calcium channel alpha-1G subunit|
           (Voltage-gated calcium channel alpha subunit Cav3.1)
           (Cav3.1c) (NBR13)
          Length = 2377

 Score = 28.5 bits (62), Expect = 9.1
 Identities = 14/30 (46%), Positives = 18/30 (60%)
 Frame = -1

Query: 195 YHWQIHAQSPFCISRWPPRQGGGRSCRSAP 106
           Y  +   +SPF  S+  PR+ G RSCRS P
Sbjct: 259 YQTENEDESPFICSQ--PRENGMRSCRSVP 286



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>CAC1G_RAT (O54898) Voltage-dependent T-type calcium channel alpha-1G subunit|
           (Voltage-gated calcium channel alpha subunit Cav3.1)
          Length = 2254

 Score = 28.5 bits (62), Expect = 9.1
 Identities = 14/30 (46%), Positives = 18/30 (60%)
 Frame = -1

Query: 195 YHWQIHAQSPFCISRWPPRQGGGRSCRSAP 106
           Y  +   +SPF  S+  PR+ G RSCRS P
Sbjct: 259 YQTENEDESPFICSQ--PRENGMRSCRSVP 286



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>UBP53_HUMAN (Q70EK8) Inactive ubiquitin carboxyl-terminal hydrolase 53|
           (Ubiquitin-specific peptidase 53)
          Length = 1073

 Score = 28.5 bits (62), Expect = 9.1
 Identities = 17/63 (26%), Positives = 33/63 (52%)
 Frame = +3

Query: 228 RLFNSSERKNPTNFQFERHVARLESRQQQQPRRCIFTTILPIEFFHTSSEPTSPEDSLDS 407
           +LF SS  + P +     H+ + + ++ ++P  C F+  L IE    +SE T     +D+
Sbjct: 792 KLFPSSSLQIPKDHNAREHIHQSDEQKLEKPNECKFSEWLNIE----NSERTGLPFHVDN 847

Query: 408 ASS 416
           ++S
Sbjct: 848 SAS 850



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>MRAZ_PSEU2 (Q4ZNY1) Protein mraZ|
          Length = 151

 Score = 28.5 bits (62), Expect = 9.1
 Identities = 24/72 (33%), Positives = 33/72 (45%), Gaps = 5/72 (6%)
 Frame = -3

Query: 412 DAESRLSSGDVGSDEVWKNSIGKIVVKIHRLGCCCCLDSRRAT*RSNWKLVGFFLSD--- 242
           DA+ RL+      DE+   S G+++V I  +  C CL        S W+L+   L D   
Sbjct: 11  DAKGRLAMPSRYRDELDSRSAGQLIVTIDAVDPCLCLYP-----LSEWELIEAKLRDLAT 65

Query: 241 --ELKRRLIFLL 212
             E  RRL  LL
Sbjct: 66  FREENRRLQRLL 77



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>MRAZ_PSE14 (Q48EE9) Protein mraZ|
          Length = 151

 Score = 28.5 bits (62), Expect = 9.1
 Identities = 24/72 (33%), Positives = 33/72 (45%), Gaps = 5/72 (6%)
 Frame = -3

Query: 412 DAESRLSSGDVGSDEVWKNSIGKIVVKIHRLGCCCCLDSRRAT*RSNWKLVGFFLSD--- 242
           DA+ RL+      DE+   S G+++V I  +  C CL        S W+L+   L D   
Sbjct: 11  DAKGRLAMPSRYRDELDSRSAGQLIVTIDAVDPCLCLYP-----LSEWELIEAKLRDLAT 65

Query: 241 --ELKRRLIFLL 212
             E  RRL  LL
Sbjct: 66  FREENRRLQRLL 77


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,893,269
Number of Sequences: 219361
Number of extensions: 1260153
Number of successful extensions: 3792
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 3646
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3787
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3072927439
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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