ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl24c12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1PHBC_CHRVI (P45370) Poly-beta-hydroxybutyrate polymerase (EC 2.3... 34 0.079
2PASK_HUMAN (Q96RG2) PAS domain-containing serine/threonine-prote... 32 0.39
3PHBC_THIVI (P45366) Poly-beta-hydroxybutyrate polymerase (EC 2.3... 30 1.1
4CP74A_ARATH (Q96242) Cytochrome P450 74A, chloroplast precursor ... 30 1.5
5DDX50_MOUSE (Q99MJ9) ATP-dependent RNA helicase DDX50 (EC 3.6.1.... 30 1.9
6EGR4_RAT (Q00911) Early growth response protein 4 (EGR-4) (Early... 29 2.5
7DDX50_HUMAN (Q9BQ39) ATP-dependent RNA helicase DDX50 (EC 3.6.1.... 29 3.3
8FOS_TETFL (Q91496) Proto-oncogene protein c-fos (Cellular oncoge... 28 4.3
9PYP2_SCHPO (P32586) Tyrosine-protein phosphatase 2 (EC 3.1.3.48)... 28 4.3
10DDX21_HUMAN (Q9NR30) Nucleolar RNA helicase 2 (EC 3.6.1.-) (Nucl... 28 4.3
11POLG_TBEVW (P14336) Genome polyprotein [Contains: Capsid protein... 28 5.7
12POLG_TBEVH (Q01299) Genome polyprotein [Contains: Capsid protein... 28 5.7
13CRU3_BRANA (P33525) Cruciferin CRU1 precursor (11S globulin) (12... 28 5.7
14PHF10_HUMAN (Q8WUB8) PHD finger protein 10 (XAP135) 28 5.7
15CD2L5_MOUSE (Q69ZA1) Cell division cycle 2-like protein kinase 5... 28 5.7
16T2_MOUSE (Q06666) Octapeptide-repeat protein T2 28 7.4
17EGR4_MOUSE (Q9WUF2) Early growth response protein 4 (EGR-4) 28 7.4
18RS5_MYCTU (P66574) 30S ribosomal protein S5 27 9.7
19RS5_MYCBO (P66575) 30S ribosomal protein S5 27 9.7
20NEB1_HUMAN (Q9ULJ8) Neurabin-1 (Neurabin-I) (Neural tissue-speci... 27 9.7
21DLGD_SALTY (Q8ZL83) 2,3-diketo-L-gulonate reductase (EC 1.1.1.13... 27 9.7
22DLGD_SALTI (Q8Z2C5) 2,3-diketo-L-gulonate reductase (EC 1.1.1.13... 27 9.7
23DLGD_SALPA (Q5PLN6) 2,3-diketo-L-gulonate reductase (EC 1.1.1.13... 27 9.7

>PHBC_CHRVI (P45370) Poly-beta-hydroxybutyrate polymerase (EC 2.3.1.-)|
           (Poly(3-hydroxybutyrate) polymerase) (PHB polymerase)
           (PHB synthase) (Poly(3-hydroxyalkanoate) polymerase)
           (PHA polymerase) (PHA synthase) (Polyhydroxyalkanoic
           acid synthase)
          Length = 355

 Score = 34.3 bits (77), Expect = 0.079
 Identities = 19/69 (27%), Positives = 33/69 (47%)
 Frame = -2

Query: 221 GQKYFAIVDKCTQKTKGTGYRQVEPWQGSRSQRGGEDGRDRLAEARRGTGQSSGGRVTMG 42
           GQKY  +VD      K   + ++E W      + GE  R  + +  +  G  +GG V  G
Sbjct: 223 GQKYVNMVDLLDDPDKVKNFLRMEKWIFDSPDQAGETFRQFIKDFYQNNGFLNGGVVLGG 282

Query: 41  EEVEKQRVS 15
           +EV+ + ++
Sbjct: 283 QEVDLKDIT 291



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>PASK_HUMAN (Q96RG2) PAS domain-containing serine/threonine-protein kinase (EC|
           2.7.11.1) (PAS-kinase) (PASKIN) (hPASK)
          Length = 1323

 Score = 32.0 bits (71), Expect = 0.39
 Identities = 17/49 (34%), Positives = 23/49 (46%)
 Frame = -2

Query: 173 GTGYRQVEPWQGSRSQRGGEDGRDRLAEARRGTGQSSGGRVTMGEEVEK 27
           G G R ++PWQG     GG+D R  +  A        GG V   +E+ K
Sbjct: 416 GCGERTLDPWQGQDPAEGGQDPRINVVLA--------GGHVVPRDEIRK 456



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>PHBC_THIVI (P45366) Poly-beta-hydroxybutyrate polymerase (EC 2.3.1.-)|
           (Poly(3-hydroxybutyrate) polymerase) (PHB polymerase)
           (PHB synthase) (Poly(3-hydroxyalkanoate) polymerase)
           (PHA polymerase) (PHA synthase) (Polyhydroxyalkanoic
           acid synthase)
          Length = 355

 Score = 30.4 bits (67), Expect = 1.1
 Identities = 18/68 (26%), Positives = 30/68 (44%)
 Frame = -2

Query: 221 GQKYFAIVDKCTQKTKGTGYRQVEPWQGSRSQRGGEDGRDRLAEARRGTGQSSGGRVTMG 42
           GQKY  +VD      K   + ++E W      + GE  R    +  +  G  +GG    G
Sbjct: 222 GQKYVNMVDMLDDPDKVKNFLRMEKWIFDSPDQAGETFRQFTKDFYQKNGFINGGVKLGG 281

Query: 41  EEVEKQRV 18
           +E++ + V
Sbjct: 282 KEIDLKNV 289



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>CP74A_ARATH (Q96242) Cytochrome P450 74A, chloroplast precursor (EC 4.2.1.92)|
           (Allene oxide synthase) (Hydroperoxide dehydrase)
          Length = 518

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 18/52 (34%), Positives = 28/52 (53%)
 Frame = -2

Query: 167 GYRQVEPWQGSRSQRGGEDGRDRLAEARRGTGQSSGGRVTMGEEVEKQRVSR 12
           G + + P    R  R G    +RLAE  R   +S+GG +TMG  +EK  +++
Sbjct: 325 GMKILFPNMVKRIGRAGHQVHNRLAEEIRSVIKSNGGELTMG-AIEKMELTK 375



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>DDX50_MOUSE (Q99MJ9) ATP-dependent RNA helicase DDX50 (EC 3.6.1.-) (DEAD box|
           protein 50) (Nucleolar protein Gu2) (Gu-beta)
          Length = 734

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 20/81 (24%), Positives = 43/81 (53%), Gaps = 1/81 (1%)
 Frame = -2

Query: 248 DTRVRQSRHGQ-KYFAIVDKCTQKTKGTGYRQVEPWQGSRSQRGGEDGRDRLAEARRGTG 72
           +T  R+ + G    F+I ++  +  KG G   + P Q  ++     +G+D +A+AR GTG
Sbjct: 125 ETLTREQKEGAFSNFSISEETIKLLKGRGVTYLFPIQ-VKTFGPVYEGKDLIAQARTGTG 183

Query: 71  QSSGGRVTMGEEVEKQRVSRK 9
           ++    + + E +++ + + K
Sbjct: 184 KTFSFAIPLIERLQRNQETIK 204



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>EGR4_RAT (Q00911) Early growth response protein 4 (EGR-4) (Early response|
           protein NGFI-C) (Nerve growth factor-induced protein C)
           (NGFI-C)
          Length = 478

 Score = 29.3 bits (64), Expect = 2.5
 Identities = 14/28 (50%), Positives = 17/28 (60%)
 Frame = +2

Query: 113 LPRLAASDCPARAPPDGNPFLSSSVYTC 196
           LPRL A D P+ A   G  FLS ++ TC
Sbjct: 28  LPRLPARDAPSAAAYPGGDFLSWALSTC 55



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>DDX50_HUMAN (Q9BQ39) ATP-dependent RNA helicase DDX50 (EC 3.6.1.-) (DEAD box|
           protein 50) (Nucleolar protein Gu2) (Gu-beta)
          Length = 737

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 20/81 (24%), Positives = 42/81 (51%), Gaps = 1/81 (1%)
 Frame = -2

Query: 248 DTRVRQSRHGQ-KYFAIVDKCTQKTKGTGYRQVEPWQGSRSQRGGEDGRDRLAEARRGTG 72
           +T  R+ + G    F I ++  +  KG G   + P Q  ++     +G+D +A+AR GTG
Sbjct: 128 ETLTREQKEGAFSNFPISEETIKLLKGRGVTYLFPIQ-VKTFGPVYEGKDLIAQARTGTG 186

Query: 71  QSSGGRVTMGEEVEKQRVSRK 9
           ++    + + E +++ + + K
Sbjct: 187 KTFSFAIPLIERLQRNQETIK 207



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>FOS_TETFL (Q91496) Proto-oncogene protein c-fos (Cellular oncogene fos)|
          Length = 374

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 20/50 (40%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
 Frame = +2

Query: 113 LPRLAASDCPARAPPDGNPFLSSSVYTCQLWQNISA---HDDFDELWCPV 253
           L  LA ++      PD N  LSSS+YT Q W+ + A     DF+ L  PV
Sbjct: 274 LDLLAKTEVETVEVPDVN--LSSSLYTAQDWEPLHATIGSSDFEPLCTPV 321



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>PYP2_SCHPO (P32586) Tyrosine-protein phosphatase 2 (EC 3.1.3.48)|
           (Protein-tyrosine phosphatase 2) (PTPase 2)
          Length = 711

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 24/72 (33%), Positives = 28/72 (38%), Gaps = 1/72 (1%)
 Frame = -2

Query: 311 EVPYKPIEELCSCGSQPWG*PDTRVRQSRHGQKYFAIVDKCTQKTKG-TGYRQVEPWQGS 135
           E+PY PI    SC S     PD         QK F IV    Q+T      R V  +  S
Sbjct: 243 EMPYFPISPAISCASSCPSTPDE--------QKNFFIVGNAPQQTPARPSLRSVPSYPSS 294

Query: 134 RSQRGGEDGRDR 99
            +QR     R R
Sbjct: 295 NNQRRPSASRVR 306



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>DDX21_HUMAN (Q9NR30) Nucleolar RNA helicase 2 (EC 3.6.1.-) (Nucleolar RNA|
           helicase II) (Nucleolar RNA helicase Gu) (RH II/Gu)
           (Gu-alpha) (DEAD box protein 21)
          Length = 783

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 20/77 (25%), Positives = 37/77 (48%)
 Frame = -2

Query: 239 VRQSRHGQKYFAIVDKCTQKTKGTGYRQVEPWQGSRSQRGGEDGRDRLAEARRGTGQSSG 60
           V Q       F I ++  +  KG G   + P Q +++      G+D +A+AR GTG++  
Sbjct: 181 VEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQ-AKTFHHVYSGKDLIAQARTGTGKTFS 239

Query: 59  GRVTMGEEVEKQRVSRK 9
             + + E++  +   RK
Sbjct: 240 FAIPLIEKLHGELQDRK 256



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>POLG_TBEVW (P14336) Genome polyprotein [Contains: Capsid protein C (Core|
            protein); Envelope protein M (Matrix protein); Major
            envelope protein E; Nonstructural protein 1 (NS1);
            Nonstructural protein 2A (NS2A); Flavivirin protease NS2B
            regulatory subunit;
          Length = 3414

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = -2

Query: 173  GTGYRQVEPWQGSRSQRGGEDGRDRLAEARRGTGQSSGGRVTM 45
            G G +  E +  S +Q   E  R  L +A  GTG +S G++T+
Sbjct: 1643 GNGLKTNETYVSSIAQGEAEKSRPNLPQAVVGTGWTSKGQITV 1685



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>POLG_TBEVH (Q01299) Genome polyprotein [Contains: Capsid protein C (Core|
            protein); Envelope protein M (Matrix protein); Major
            envelope protein E; Nonstructural protein 1 (NS1);
            Nonstructural protein 2A (NS2A); Flavivirin protease NS2B
            regulatory subunit;
          Length = 3414

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 15/43 (34%), Positives = 23/43 (53%)
 Frame = -2

Query: 173  GTGYRQVEPWQGSRSQRGGEDGRDRLAEARRGTGQSSGGRVTM 45
            G G +  E +  S +Q   E  R  L +A  GTG +S G++T+
Sbjct: 1643 GNGLKTNETYVSSIAQGEAEKSRPNLPQAVVGTGWTSKGQITV 1685



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>CRU3_BRANA (P33525) Cruciferin CRU1 precursor (11S globulin) (12S storage|
           protein) [Contains: Cruciferin CRU1 alpha chain;
           Cruciferin CRU1 beta chain]
          Length = 509

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 14/51 (27%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
 Frame = -2

Query: 167 GYRQVEPWQGSRSQRG--GEDGRDRLAEARRGTGQSSGGRVTMGEEVEKQR 21
           G +Q +PWQG + Q+G  G+ G+    + ++G     G +   G++ ++Q+
Sbjct: 125 GQQQGQPWQGQQGQQGQQGQQGQ----QGQQGQQGQQGQQGQQGQQGQQQQ 171



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>PHF10_HUMAN (Q8WUB8) PHD finger protein 10 (XAP135)|
          Length = 410

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 13/27 (48%), Positives = 15/27 (55%)
 Frame = -2

Query: 119 GEDGRDRLAEARRGTGQSSGGRVTMGE 39
           GEDGR       +GT  SS G V+ GE
Sbjct: 216 GEDGRGDEKRKNKGTSDSSSGNVSEGE 242



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>CD2L5_MOUSE (Q69ZA1) Cell division cycle 2-like protein kinase 5 (EC 2.7.11.22)|
           (CDC2-related protein kinase 5)
          Length = 1511

 Score = 28.1 bits (61), Expect = 5.7
 Identities = 14/39 (35%), Positives = 17/39 (43%)
 Frame = -2

Query: 125 RGGEDGRDRLAEARRGTGQSSGGRVTMGEEVEKQRVSRK 9
           R G  G +R   A+ G+  SSGGR            SRK
Sbjct: 234 RHGHSGEERAEAAKSGSSSSSGGRRKSASATSSSSSSRK 272



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>T2_MOUSE (Q06666) Octapeptide-repeat protein T2|
          Length = 185

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 19/55 (34%), Positives = 27/55 (49%), Gaps = 4/55 (7%)
 Frame = -2

Query: 161 RQVEPWQGSRSQRGGEDGRDRLAEARRG-TGQSSGGRVT---MGEEVEKQRVSRK 9
           R+ E  +G  ++R  E  R+R  E +RG   +   GR      G E EKQR  R+
Sbjct: 97  REAERQRGREAEREAERQRERQRERQRGREAERQRGREAERQRGREAEKQRGERQ 151



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>EGR4_MOUSE (Q9WUF2) Early growth response protein 4 (EGR-4)|
          Length = 478

 Score = 27.7 bits (60), Expect = 7.4
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = +2

Query: 113 LPRLAASDCPARAPPDGNPFLSSSVYTC 196
           LPRL A D P+ A   G  FLS ++ +C
Sbjct: 28  LPRLPARDAPSAAAYPGGDFLSWALSSC 55



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>RS5_MYCTU (P66574) 30S ribosomal protein S5|
          Length = 220

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 11/16 (68%), Positives = 12/16 (75%)
 Frame = -1

Query: 63 GRESDDGRGSREAEGE 16
          GR  D GRGSRE +GE
Sbjct: 23 GRRRDSGRGSRERDGE 38



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>RS5_MYCBO (P66575) 30S ribosomal protein S5|
          Length = 220

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 11/16 (68%), Positives = 12/16 (75%)
 Frame = -1

Query: 63 GRESDDGRGSREAEGE 16
          GR  D GRGSRE +GE
Sbjct: 23 GRRRDSGRGSRERDGE 38



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>NEB1_HUMAN (Q9ULJ8) Neurabin-1 (Neurabin-I) (Neural tissue-specific F-actin|
           binding protein I) (Protein phosphatase 1 regulatory
           subunit 9A)
          Length = 1098

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 12/31 (38%), Positives = 16/31 (51%)
 Frame = -2

Query: 185 QKTKGTGYRQVEPWQGSRSQRGGEDGRDRLA 93
           +K K  G    + W GS+S RG  D  D L+
Sbjct: 162 KKEKAGGSEPQDEWGGSKSNRGSTDSLDSLS 192



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>DLGD_SALTY (Q8ZL83) 2,3-diketo-L-gulonate reductase (EC 1.1.1.130) (2,3-DKG|
           reductase) (3-dehydro-L-gulonate 2-dehydrogenase)
          Length = 332

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 25/93 (26%), Positives = 37/93 (39%), Gaps = 21/93 (22%)
 Frame = -3

Query: 325 ELKIKKYHINRSRNCV----------------LAALNHGVDRTPEFVKVVMGRNILP*LT 194
           ELK   Y + RSRN                      +HGV+R P F++ +   +I+P   
Sbjct: 7   ELKGAFYRVLRSRNIAEDTADECAEMFARTTESGVYSHGVNRFPRFIQQLDNGDIIP--- 63

Query: 193 SVHRRRKERVT-----VRWSPGRAVARSEAGKM 110
                + +RVT      +W   RA+    A KM
Sbjct: 64  ---DAKPQRVTSLGAIEQWDAQRAIGNLTAKKM 93



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>DLGD_SALTI (Q8Z2C5) 2,3-diketo-L-gulonate reductase (EC 1.1.1.130) (2,3-DKG|
           reductase) (3-dehydro-L-gulonate 2-dehydrogenase)
          Length = 332

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 25/93 (26%), Positives = 37/93 (39%), Gaps = 21/93 (22%)
 Frame = -3

Query: 325 ELKIKKYHINRSRNCV----------------LAALNHGVDRTPEFVKVVMGRNILP*LT 194
           ELK   Y + RSRN                      +HGV+R P F++ +   +I+P   
Sbjct: 7   ELKGAFYRVLRSRNIAEDTADACAEMFARTTESGVYSHGVNRFPRFIQQLDNGDIIP--- 63

Query: 193 SVHRRRKERVT-----VRWSPGRAVARSEAGKM 110
                + +RVT      +W   RA+    A KM
Sbjct: 64  ---DAKPQRVTSLGAIEQWDAQRAIGNLTAKKM 93



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>DLGD_SALPA (Q5PLN6) 2,3-diketo-L-gulonate reductase (EC 1.1.1.130) (2,3-DKG|
           reductase) (3-dehydro-L-gulonate 2-dehydrogenase)
          Length = 332

 Score = 27.3 bits (59), Expect = 9.7
 Identities = 25/93 (26%), Positives = 37/93 (39%), Gaps = 21/93 (22%)
 Frame = -3

Query: 325 ELKIKKYHINRSRNCV----------------LAALNHGVDRTPEFVKVVMGRNILP*LT 194
           ELK   Y + RSRN                      +HGV+R P F++ +   +I+P   
Sbjct: 7   ELKGAFYRVLRSRNIAEDTADACAEMFARTTESGVYSHGVNRFPRFIQQLDNGDIIP--- 63

Query: 193 SVHRRRKERVT-----VRWSPGRAVARSEAGKM 110
                + +RVT      +W   RA+    A KM
Sbjct: 64  ---DAKPQRVTSLGAIEQWDAQRAIGNLTAKKM 93


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,582,849
Number of Sequences: 219361
Number of extensions: 776518
Number of successful extensions: 2966
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 2884
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2964
length of database: 80,573,946
effective HSP length: 88
effective length of database: 61,270,178
effective search space used: 1470484272
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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