ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl23b10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1LOX21_HORVU (P93184) Lipoxygenase 2.1, chloroplast precursor (EC... 129 2e-30
2LOX22_HORVU (Q8GSM3) Lipoxygenase 2.2, chloroplast precursor (EC... 41 6e-04
3PO121_MOUSE (Q8K3Z9) Nuclear envelope pore membrane protein POM ... 32 0.28
4DUSC_AZOVI (P96192) tRNA-dihydrouridine synthase C (EC 1.-.-.-) 25 2.6
5SUBT_BACLI (P00780) Subtilisin Carlsberg precursor (EC 3.4.21.62) 29 3.1
6SYN3_RAT (O70441) Synapsin-3 (Synapsin III) 28 5.2
7SYN3_MOUSE (Q8JZP2) Synapsin-3 (Synapsin III) 28 5.2
8SC10A_RAT (Q62968) Sodium channel protein type 10 alpha subunit ... 28 5.2
9PO121_RAT (P52591) Nuclear envelope pore membrane protein POM 12... 28 5.2
10ACSA2_PSEPK (Q88DW6) Acetyl-coenzyme A synthetase 2 (EC 6.2.1.1)... 28 5.2
11GUAA_LEGPL (Q5WVX4) GMP synthase [glutamine-hydrolyzing] (EC 6.3... 28 6.8
12GUAA_LEGPH (Q5ZUS0) GMP synthase [glutamine-hydrolyzing] (EC 6.3... 28 6.8
13GUAA_LEGPA (Q5X4I9) GMP synthase [glutamine-hydrolyzing] (EC 6.3... 28 6.8
14TCGAP_HUMAN (O14559) TC10/CDC42 GTPase-activating protein (Sorti... 28 6.8
15TBX21_MOUSE (Q9JKD8) T-box transcription factor TBX21 (T-box pro... 27 8.9
16BAG4_HUMAN (O95429) BAG family molecular chaperone regulator 4 (... 27 8.9

>LOX21_HORVU (P93184) Lipoxygenase 2.1, chloroplast precursor (EC 1.13.11.12)|
           (LOX-100) (LOX2:Hv:1)
          Length = 936

 Score =  129 bits (323), Expect = 2e-30
 Identities = 64/88 (72%), Positives = 64/88 (72%)
 Frame = +3

Query: 141 MLAATKPLVGGACXXXXXXXXXXTFVVPEARRKPGNGRRTSVSKVGXXXXXXXXXXXXXL 320
           ML ATKPLVGGAC          TFVVPEARRKPGNGRRTSVSKVG             L
Sbjct: 1   MLTATKPLVGGACAAPSSSARRRTFVVPEARRKPGNGRRTSVSKVGSTSTSTTTTTTTTL 60

Query: 321 SADSNGAAVGTVTRPDVHVQDRTHATEM 404
           SADSNGAAVGTVTRPDVHVQDRTHATEM
Sbjct: 61  SADSNGAAVGTVTRPDVHVQDRTHATEM 88



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>LOX22_HORVU (Q8GSM3) Lipoxygenase 2.2, chloroplast precursor (EC 1.13.11.12)|
           (LOX2:Hv:2)
          Length = 932

 Score = 41.2 bits (95), Expect = 6e-04
 Identities = 32/89 (35%), Positives = 37/89 (41%), Gaps = 1/89 (1%)
 Frame = +3

Query: 141 MLAATKPLVGGACXXXXXXXXXXTFVVPEARRKPG-NGRRTSVSKVGXXXXXXXXXXXXX 317
           M  ATKPLVG             +F+V EARRKP  N RRT V                 
Sbjct: 1   MQTATKPLVGARAVPLSRRA---SFLVAEARRKPSTNARRTRVG------STSTTTTTTT 51

Query: 318 LSADSNGAAVGTVTRPDVHVQDRTHATEM 404
           +  D NG A+ TV +P  H  D     EM
Sbjct: 52  ILTDVNGPALTTVAKPG-HQYDLKQTVEM 79



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>PO121_MOUSE (Q8K3Z9) Nuclear envelope pore membrane protein POM 121 (Pore|
           membrane protein of 121 kDa)
          Length = 1200

 Score = 32.3 bits (72), Expect = 0.28
 Identities = 16/33 (48%), Positives = 18/33 (54%)
 Frame = -3

Query: 240 VSGEPRAPRTSSAGPTTTARHTRPRLAAWSPPA 142
           +S EPR PR  S+      RH RP L A  PPA
Sbjct: 71  LSREPRGPRALSSFVRDARRHPRPALTASPPPA 103



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>DUSC_AZOVI (P96192) tRNA-dihydrouridine synthase C (EC 1.-.-.-)|
          Length = 309

 Score = 25.0 bits (53), Expect(2) = 2.6
 Identities = 23/65 (35%), Positives = 28/65 (43%), Gaps = 7/65 (10%)
 Frame = -3

Query: 180 HTRPRLAAWSPPASW-----VRYGVG--YCATRWGGRLHRLGWFRPS*TVQPCPGRSGKE 22
           H R ++  + PPASW     VR  V     A R  GR  R+G          C   SG E
Sbjct: 167 HARTKVEGYRPPASWEWLARVREAVAVPVYANRKSGR-RRIG----------CREISGVE 215

Query: 21  EVMEG 7
           +VM G
Sbjct: 216 DVMLG 220



 Score = 22.7 bits (47), Expect(2) = 2.6
 Identities = 11/27 (40%), Positives = 13/27 (48%)
 Frame = -2

Query: 256 RRPLPGFRRASGTTNVLRRADDDGAAH 176
           R+  PG R A G   V R   + G AH
Sbjct: 137 RQDAPGVRPARGRPGVRRALVEGGVAH 163



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>SUBT_BACLI (P00780) Subtilisin Carlsberg precursor (EC 3.4.21.62)|
          Length = 379

 Score = 28.9 bits (63), Expect = 3.1
 Identities = 21/71 (29%), Positives = 28/71 (39%), Gaps = 1/71 (1%)
 Frame = -2

Query: 355 TVPTAAPLESADKXXXXXXXXXXXXLPTLETLVRRPLPGFRRASGTTNVLRRA-DDDGAA 179
           TVP   PL  ADK            +  L+T ++   P      G + V   A + DG  
Sbjct: 108 TVPYGIPLIKADKVQAQGFKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEAYNTDGNG 167

Query: 178 HAPPTSGLVAA 146
           H    +G VAA
Sbjct: 168 HGTHVAGTVAA 178



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>SYN3_RAT (O70441) Synapsin-3 (Synapsin III)|
          Length = 579

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -3

Query: 228 PRAPRTSSAGPTTTARHTRPRLAAWSPPAS 139
           P +  +S A P T  RH +P  A++S P S
Sbjct: 28  PESSSSSPASPATERRHPQPLAASFSSPGS 57



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>SYN3_MOUSE (Q8JZP2) Synapsin-3 (Synapsin III)|
          Length = 579

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -3

Query: 228 PRAPRTSSAGPTTTARHTRPRLAAWSPPAS 139
           P +  +S A P T  RH +P  A++S P S
Sbjct: 28  PESSSSSPASPATERRHPQPLAASFSSPGS 57



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>SC10A_RAT (Q62968) Sodium channel protein type 10 alpha subunit (Sodium|
           channel protein type X alpha subunit) (Voltage-gated
           sodium channel alpha subunit Nav1.8) (Peripheral nerve
           sodium channel 3) (Sensory neuron sodium channel)
          Length = 1956

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 17/55 (30%), Positives = 21/55 (38%)
 Frame = +2

Query: 62  LGRNQPRRCNLPPHRVAQ*PTP*RTHDAGGDQAASRGRVCRAVVVGPAEDVRGAR 226
           LGR   +   LP   + Q P P R H   G      G +      GPA D  G +
Sbjct: 538 LGRGAGQTGPLPRSPLPQSPNPGRRHGEEGQLGVPTGELTAGAPEGPALDTTGQK 592



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>PO121_RAT (P52591) Nuclear envelope pore membrane protein POM 121 (Pore|
           membrane protein of 121 kDa) (P145)
          Length = 1199

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 15/33 (45%), Positives = 18/33 (54%)
 Frame = -3

Query: 240 VSGEPRAPRTSSAGPTTTARHTRPRLAAWSPPA 142
           +S EPR PR  S+    + RH RP L A   PA
Sbjct: 71  LSREPRGPRGLSSFVRESRRHPRPALTASPLPA 103



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>ACSA2_PSEPK (Q88DW6) Acetyl-coenzyme A synthetase 2 (EC 6.2.1.1) (Acetate--CoA|
           ligase 2) (Acyl-activating enzyme 2)
          Length = 644

 Score = 28.1 bits (61), Expect = 5.2
 Identities = 12/35 (34%), Positives = 18/35 (51%)
 Frame = -3

Query: 108 TRWGGRLHRLGWFRPS*TVQPCPGRSGKEEVMEGS 4
           T W  +  RL W +P  +VQ C   +GK    +G+
Sbjct: 37  TFWAEQAKRLDWIKPWSSVQQCDLHTGKARWFDGA 71



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>GUAA_LEGPL (Q5WVX4) GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)|
           (Glutamine amidotransferase) (GMP synthetase)
          Length = 525

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 4/45 (8%)
 Frame = -3

Query: 207 SAGPTTTARHTRPRLAAW----SPPASWVRYGVGYCATRWGGRLH 85
           S GP+T      PR   W      P   + YG+   A + GG++H
Sbjct: 56  SGGPSTVTHDANPRAPQWLFESDLPLLGICYGMQTMAVQLGGQVH 100



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>GUAA_LEGPH (Q5ZUS0) GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)|
           (Glutamine amidotransferase) (GMP synthetase)
          Length = 525

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 4/45 (8%)
 Frame = -3

Query: 207 SAGPTTTARHTRPRLAAW----SPPASWVRYGVGYCATRWGGRLH 85
           S GP+T      PR   W      P   + YG+   A + GG++H
Sbjct: 56  SGGPSTVTHDANPRAPQWLFDSGLPLLGICYGMQTMAVQLGGQVH 100



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>GUAA_LEGPA (Q5X4I9) GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)|
           (Glutamine amidotransferase) (GMP synthetase)
          Length = 525

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 14/45 (31%), Positives = 20/45 (44%), Gaps = 4/45 (8%)
 Frame = -3

Query: 207 SAGPTTTARHTRPRLAAW----SPPASWVRYGVGYCATRWGGRLH 85
           S GP+T      PR   W      P   + YG+   A + GG++H
Sbjct: 56  SGGPSTVTHDANPRAPQWLFESDLPLLGICYGMQTMAVQLGGQVH 100



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>TCGAP_HUMAN (O14559) TC10/CDC42 GTPase-activating protein (Sorting nexin 26)|
          Length = 1287

 Score = 27.7 bits (60), Expect = 6.8
 Identities = 20/56 (35%), Positives = 23/56 (41%), Gaps = 2/56 (3%)
 Frame = -3

Query: 228  PRAPRTSSA--GPTTTARHTRPRLAAWSPPASWVRYGVGYCATRWGGRLHRLGWFR 67
            PR P+   A  GP T  R   P    W PP   + Y     A   GG LHR   +R
Sbjct: 1204 PRLPQKQRAPWGPRTPHRVPGP----WGPPEPLLLYRAAPPAYGRGGELHRGSLYR 1255



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>TBX21_MOUSE (Q9JKD8) T-box transcription factor TBX21 (T-box protein 21)|
           (Transcription factor TBLYM) (T-cell-specific T-box
           transcription factor T-bet)
          Length = 530

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 20/64 (31%), Positives = 27/64 (42%)
 Frame = -3

Query: 210 SSAGPTTTARHTRPRLAAWSPPASWVRYGVGYCATRWGGRLHRLGWFRPS*TVQPCPGRS 31
           S+ GPT      +  LA   P A W        A ++  ++   GWFRP  T+   PG  
Sbjct: 410 SAPGPTVPYYRGQDVLA---PGAGWP------VAPQYPPKMSPAGWFRPMRTLPMDPGLG 460

Query: 30  GKEE 19
             EE
Sbjct: 461 SSEE 464



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>BAG4_HUMAN (O95429) BAG family molecular chaperone regulator 4|
           (BCL2-associated athanogene 4) (BAG-4) (Silencer of
           death domains)
          Length = 457

 Score = 27.3 bits (59), Expect = 8.9
 Identities = 16/41 (39%), Positives = 18/41 (43%)
 Frame = -3

Query: 150 PPASWVRYGVGYCATRWGGRLHRLGWFRPS*TVQPCPGRSG 28
           PP SW   G G   T W G       + PS    P PGR+G
Sbjct: 46  PPISWRVRGGGPAETTWLGEGGGGDGYYPSGGAWPEPGRAG 86


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,452,154
Number of Sequences: 219361
Number of extensions: 924889
Number of successful extensions: 2582
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 2531
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2582
length of database: 80,573,946
effective HSP length: 110
effective length of database: 56,444,236
effective search space used: 1354661664
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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