ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl20c09
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1PRP8_MOUSE (Q99PV0) Pre-mRNA-processing-splicing factor 8 (Splic... 280 2e-75
2PRP8_HUMAN (Q6P2Q9) Pre-mRNA-processing-splicing factor 8 (Splic... 280 2e-75
3PRP8_CAEEL (P34369) Pre-mRNA-splicing factor 8 homolog 276 2e-74
4PRP8_YEAST (P33334) Pre-mRNA-splicing factor 8 221 1e-57
5SYG_METJA (Q57681) Glycyl-tRNA synthetase (EC 6.1.1.14) (Glycine... 31 1.9
6TLE6_MOUSE (Q9WVB3) Transducin-like enhancer protein 6 (Groucho-... 30 3.3
7TCPH_TETTH (P54410) T-complex protein 1 subunit eta (TCP-1-eta) ... 30 4.3
8GIT2_HUMAN (Q14161) ARF GTPase-activating protein GIT2 (G protei... 30 4.3
9FREM1_MOUSE (Q684R7) FRAS1-related extracellular matrix protein ... 30 4.3
10LY41_SYNY3 (P74269) Putative AIPM/Hcit synthase family transfera... 29 5.6
11YCOM_RHORU (P72315) Hypothetical 49.9 kDa protein in cooM 5'region 29 7.3
12NIA_BEABA (P43100) Nitrate reductase [NADPH] (EC 1.7.1.3) (NR) 29 7.3
13YGMH_SCHPO (O42965) Hypothetical protein C19G7.17 in chromosome II 28 9.6
14YKZ1_YEAST (Q02209) Hypothetical 41.3 kDa protein in FOX2-YPT52 ... 28 9.6
15MTMR1_MOUSE (Q9Z2C4) Myotubularin-related protein 1 (EC 3.1.3.-) 28 9.6

>PRP8_MOUSE (Q99PV0) Pre-mRNA-processing-splicing factor 8 (Splicing factor Prp8)|
          Length = 2335

 Score =  280 bits (715), Expect = 2e-75
 Identities = 133/159 (83%), Positives = 145/159 (91%)
 Frame = +3

Query: 3    QRYLTEHPDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEW 182
            QRYLTEHPDPNNEN+VGYNNKKCWPRDARMRLMKHDVNLGR+VFWD+KNRLPRS+TT++W
Sbjct: 1111 QRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQW 1170

Query: 183  ENGFVSVYSKDNPNLLFSMSGFEVRILPKIRMTQEAFSNTKDGVWNLQNEQTKERTAIAF 362
            EN FVSVYSKDNPNLLF+M GFE RILPK R + E F++ KDGVWNLQNE TKERTA  F
Sbjct: 1171 ENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTH-KDGVWNLQNEVTKERTAQCF 1229

Query: 363  LRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALI 479
            LRVDDE M+ F NRVRQILM+SGSTTFTKIVNKWNTALI
Sbjct: 1230 LRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALI 1268



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>PRP8_HUMAN (Q6P2Q9) Pre-mRNA-processing-splicing factor 8 (Splicing factor Prp8)|
            (PRP8 homolog) (220 kDa U5 snRNP-specific protein) (p220)
          Length = 2335

 Score =  280 bits (715), Expect = 2e-75
 Identities = 133/159 (83%), Positives = 145/159 (91%)
 Frame = +3

Query: 3    QRYLTEHPDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEW 182
            QRYLTEHPDPNNEN+VGYNNKKCWPRDARMRLMKHDVNLGR+VFWD+KNRLPRS+TT++W
Sbjct: 1111 QRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQW 1170

Query: 183  ENGFVSVYSKDNPNLLFSMSGFEVRILPKIRMTQEAFSNTKDGVWNLQNEQTKERTAIAF 362
            EN FVSVYSKDNPNLLF+M GFE RILPK R + E F++ KDGVWNLQNE TKERTA  F
Sbjct: 1171 ENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTH-KDGVWNLQNEVTKERTAQCF 1229

Query: 363  LRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALI 479
            LRVDDE M+ F NRVRQILM+SGSTTFTKIVNKWNTALI
Sbjct: 1230 LRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALI 1268



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>PRP8_CAEEL (P34369) Pre-mRNA-splicing factor 8 homolog|
          Length = 2329

 Score =  276 bits (707), Expect = 2e-74
 Identities = 131/159 (82%), Positives = 142/159 (89%)
 Frame = +3

Query: 3    QRYLTEHPDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEW 182
            QRYLTEHPDPNNEN+VGYNNKKCWPRDARMRLMKHDVNLGR+VFWD+KNRLPRSITT+EW
Sbjct: 1103 QRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSITTVEW 1162

Query: 183  ENGFVSVYSKDNPNLLFSMSGFEVRILPKIRMTQEAFSNTKDGVWNLQNEQTKERTAIAF 362
            EN FVSVYSKDNPN+LF MSGFE RILPK R   E F + +DGVWNLQNE TKERTA  F
Sbjct: 1163 ENSFVSVYSKDNPNMLFDMSGFECRILPKCRTANEEFVH-RDGVWNLQNEVTKERTAQCF 1221

Query: 363  LRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALI 479
            L+VD+E +  F NR+RQILMSSGSTTFTKIVNKWNTALI
Sbjct: 1222 LKVDEESLSKFHNRIRQILMSSGSTTFTKIVNKWNTALI 1260



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>PRP8_YEAST (P33334) Pre-mRNA-splicing factor 8|
          Length = 2413

 Score =  221 bits (562), Expect = 1e-57
 Identities = 101/157 (64%), Positives = 136/157 (86%)
 Frame = +3

Query: 9    YLTEHPDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWEN 188
            YL E+PDPN EN +GYNN+KCWP+D+RMRL++ DVNLGR+VFW++++R+P S+T+++WEN
Sbjct: 1186 YLAENPDPNFENSIGYNNRKCWPKDSRMRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWEN 1245

Query: 189  GFVSVYSKDNPNLLFSMSGFEVRILPKIRMTQEAFSNTKDGVWNLQNEQTKERTAIAFLR 368
             FVSVYSK+NPNLLFSM GFEVRILP+ RM +E  SN  +GVW+L +E+TK+RTA A+L+
Sbjct: 1246 AFVSVYSKNNPNLLFSMCGFEVRILPRQRM-EEVVSN-DEGVWDLVDERTKQRTAKAYLK 1303

Query: 369  VDDEHMKVFENRVRQILMSSGSTTFTKIVNKWNTALI 479
            V +E +K F++R+R ILM+SGSTTFTK+  KWNT+LI
Sbjct: 1304 VSEEEIKKFDSRIRGILMASGSTTFTKVAAKWNTSLI 1340



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>SYG_METJA (Q57681) Glycyl-tRNA synthetase (EC 6.1.1.14) (Glycine--tRNA|
           ligase) (GlyRS)
          Length = 577

 Score = 30.8 bits (68), Expect = 1.9
 Identities = 17/49 (34%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = -3

Query: 329 ILQVPYSIFCVAECLLSHTYL----RQYTDLKPRHAKEQVWVILAVYRD 195
           +++  Y I  +  CLL H+Y     R Y DLKP  A  + +V+  V +D
Sbjct: 445 VIEPSYGIDRITYCLLEHSYREEEDRVYLDLKPSIAPIKAYVLPLVNKD 493



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>TLE6_MOUSE (Q9WVB3) Transducin-like enhancer protein 6 (Groucho-related|
           protein 6)
          Length = 581

 Score = 30.0 bits (66), Expect = 3.3
 Identities = 31/142 (21%), Positives = 59/142 (41%), Gaps = 3/142 (2%)
 Frame = +3

Query: 24  PDPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKN-RLPRSITTLEWENGFVS 200
           P P  +   G  +K   P      +++   N   +  W ++   +P+++         V 
Sbjct: 236 PQPETQESSGRADKFLKPLSWGSEVLESSCNQPSTALWQLERFTVPQALQK-------VR 288

Query: 201 VYSKDNPNLLFSMSGFEVRILPKIRMTQEAFSNTKDG--VWNLQNEQTKERTAIAFLRVD 374
           V       L+ ++S F          T+  F+ ++ G  VWNL N+  ++R   + L+  
Sbjct: 289 VLKHQELLLVVAVSSF----------TRHVFTCSQSGIKVWNLVNQVAEDRDPESHLKCS 338

Query: 375 DEHMKVFENRVRQILMSSGSTT 440
            +  KV+   +R  L+SS S T
Sbjct: 339 VQDNKVY---LRTCLLSSNSRT 357



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>TCPH_TETTH (P54410) T-complex protein 1 subunit eta (TCP-1-eta) (CCT-eta)|
           (Fragment)
          Length = 353

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 28/109 (25%), Positives = 45/109 (41%), Gaps = 8/109 (7%)
 Frame = -1

Query: 454 TILVNVVDPDDIRICLTR---FSNTFMCSSSTLKNAIAVLSFVCSFCKFHTPSFVLLNAS 284
           T+  N++D D I I +      +++F+ S    K   +   F     KF+ P   LLN  
Sbjct: 126 TLETNLLDKDLIGIKMVTGGSVTDSFLVSGVAFKKTFSYAGFEQQPKKFNNPKICLLNIE 185

Query: 283 *VIRI---FGSIRTSNPDMLKSRFGL--SLLYTETKPFSHSNVVILLGR 152
             ++       IR  NPD  KS       L+Y + +    S   I+L +
Sbjct: 186 LELKAEKENAEIRIENPDDYKSIVDAEWELIYEKLRKIVESGANIVLSK 234



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>GIT2_HUMAN (Q14161) ARF GTPase-activating protein GIT2 (G protein-coupled|
           receptor kinase-interactor 2) (GRK-interacting protein
           2) (Cool-interacting tyrosine-phosphorylated protein 2)
           (CAT2) (CAT-2)
          Length = 759

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 13/36 (36%), Positives = 19/36 (52%)
 Frame = +3

Query: 75  PRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEW 182
           P ++RMRL     ++GRS      + LP   +TL W
Sbjct: 534 PEESRMRLQPFPAHIGRSALVTSSSSLPSFPSTLSW 569



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>FREM1_MOUSE (Q684R7) FRAS1-related extracellular matrix protein 1 precursor|
           (QBRICK protein)
          Length = 2191

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 23/64 (35%), Positives = 34/64 (53%), Gaps = 8/64 (12%)
 Frame = -1

Query: 235 LKSRFGLSLLYTETKP--FSHSNVVILLGRRFFI------SQNTLLPRLTSCFMSLILAS 80
           ++SR G  L+YT T+P  FS S+  +  G+ F +      ++N   P L+S    LIL S
Sbjct: 677 MESRDG-ELIYTVTRPPCFSFSHRHLDAGKLFMVDSIPKLTKNPTAPGLSSFTQGLILIS 735

Query: 79  LGQH 68
             QH
Sbjct: 736 ANQH 739



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>LY41_SYNY3 (P74269) Putative AIPM/Hcit synthase family transferase sll1564 (EC|
           2.3.3.-)
          Length = 547

 Score = 29.3 bits (64), Expect = 5.6
 Identities = 13/51 (25%), Positives = 24/51 (47%)
 Frame = +3

Query: 315 WNLQNEQTKERTAIAFLRVDDEHMKVFENRVRQILMSSGSTTFTKIVNKWN 467
           W LQ E  ++   +AF      H  V  +++ Q ++S+G+   T     W+
Sbjct: 62  WQLQEEPLEQAEIVAFCSTRRPHKAVETDKMLQAILSAGTRWVTIFGKSWD 112



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>YCOM_RHORU (P72315) Hypothetical 49.9 kDa protein in cooM 5'region|
          Length = 463

 Score = 28.9 bits (63), Expect = 7.3
 Identities = 17/48 (35%), Positives = 21/48 (43%)
 Frame = +3

Query: 108 DVNLGRSVFWDMKNRLPRSITTLEWENGFVSVYSKDNPNLLFSMSGFE 251
           D+  GR V W M  RL      L  EN F    +   PN L +  GF+
Sbjct: 385 DIRNGRDVEWAMTTRLDAKTGILVIENAFGHGLNPTFPNYLGTKVGFD 432



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>NIA_BEABA (P43100) Nitrate reductase [NADPH] (EC 1.7.1.3) (NR)|
          Length = 894

 Score = 28.9 bits (63), Expect = 7.3
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = -3

Query: 269 LRQYTDLKPRHAKEQVWVILAVYRD 195
           +R YT L   HAK Q+ +++ +YRD
Sbjct: 691 IRAYTPLSETHAKGQLDILIKIYRD 715



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>YGMH_SCHPO (O42965) Hypothetical protein C19G7.17 in chromosome II|
          Length = 475

 Score = 28.5 bits (62), Expect = 9.6
 Identities = 28/106 (26%), Positives = 45/106 (42%), Gaps = 7/106 (6%)
 Frame = -1

Query: 409 LTRFSNTFMCSSSTL--KNAIAVLSFVCSFCKFHTPSFVLLNAS*VIRIFGSIRTSNPDM 236
           L  F N F    S L     ++ + F+     F T  + L      IRI   IR+S    
Sbjct: 224 LFSFKNKFSSLRSILFRSEGLSFVQFLVCIAVFATFMYTLN-----IRIDVPIRSSRVRG 278

Query: 235 LKSRFGLSLLYTETKPFSH-----SNVVILLGRRFFISQNTLLPRL 113
           ++  F L LLYT   P  +     S++++     + +  N+L+ RL
Sbjct: 279 VRQNFPLKLLYTSVIPLIYFYSILSHLLVFAYALYSLCPNSLITRL 324



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>YKZ1_YEAST (Q02209) Hypothetical 41.3 kDa protein in FOX2-YPT52 intergenic|
           region
          Length = 353

 Score = 28.5 bits (62), Expect = 9.6
 Identities = 20/109 (18%), Positives = 51/109 (46%), Gaps = 3/109 (2%)
 Frame = +3

Query: 33  NNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWENGFVSVYSK 212
           N++    Y+ K+ W ++   ++ +    L    FW   +++ +SI   + +    ++   
Sbjct: 124 NDQRFKNYD-KRAWLQNCLEKMNRDLSKLNVEQFWSQYDKICQSIAKQKKKQEQFNMEVF 182

Query: 213 DNPNLLFSMSGFEVRILPKIRM---TQEAFSNTKDGVWNLQNEQTKERT 350
           DN   + S++  + ++L   R+   T + + N+    +N+Q +  KE +
Sbjct: 183 DNFKNIVSIAIIKTKVLSNKRLLTTTLKNYHNSMKKKYNIQEQNLKENS 231



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>MTMR1_MOUSE (Q9Z2C4) Myotubularin-related protein 1 (EC 3.1.3.-)|
          Length = 669

 Score = 28.5 bits (62), Expect = 9.6
 Identities = 30/137 (21%), Positives = 58/137 (42%), Gaps = 2/137 (1%)
 Frame = +3

Query: 27  DPNNENMVGYNNKKCWPRDARMRLMKHDVNLGRSVFWDMKNRLPRSITTLEWENGFVSVY 206
           D N     GY N+  +P    + L  H++++ R     +K  +  SI    W +     +
Sbjct: 353 DTNKAKGGGYENESAYPNAELIFLEIHNIHVMRESLRKLKEIVYPSIDESHWLSNVDGTH 412

Query: 207 SKDNPNLLFSMSGFEVRILPKIRMTQEAFSNTKDGVWNLQNEQTKERTAIAFLRVDDEH- 383
             +   +L + +   VRI  KI   + +        W    ++T + T++A L +D  + 
Sbjct: 413 WLEYIRVLLAGA---VRIADKIESGKTSVVIHCSDGW----DRTSQLTSLAMLMLDSYYR 465

Query: 384 -MKVFENRVRQILMSSG 431
            +K FE  + +  +S G
Sbjct: 466 TIKGFEALIEKEWISFG 482


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,050,954
Number of Sequences: 219361
Number of extensions: 1509530
Number of successful extensions: 3697
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 3621
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3692
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3246866728
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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