ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl20c05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1IF3A_MAIZE (Q9XHR2) Eukaryotic translation initiation factor 3 s... 225 4e-59
2IF3A_TOBAC (Q40554) Eukaryotic translation initiation factor 3 s... 206 3e-53
3IF3A_ARATH (Q9LD55) Eukaryotic translation initiation factor 3 s... 203 2e-52
4IF3A_MOUSE (P23116) Eukaryotic translation initiation factor 3 s... 137 2e-32
5IF3A_HUMAN (Q14152) Eukaryotic translation initiation factor 3 s... 136 3e-32
6IF3A_CAEEL (P34339) Probable eukaryotic translation initiation f... 119 5e-27
7IF3A_SCHPO (O74760) Probable eukaryotic translation initiation f... 103 3e-22
8IF3A_YEAST (P38249) Eukaryotic translation initiation factor 3 1... 88 1e-17
9RGP1_XENLA (O13066) Ran GTPase-activating protein 1 32 1.1
10BPAEB_HUMAN (Q8WXK8) Bullous pemphigoid antigen 1, isoform 7 (Bu... 32 1.1
11IF2A_THEAC (Q9HIX3) Translation initiation factor 2 alpha subuni... 30 2.5
12Y1002_RHIME (Q52969) Hypothetical sensor-like histidine kinase R... 30 2.5
13RAD50_PYRAB (Q9UZC8) DNA double-strand break repair rad50 ATPase 30 3.3
14SWR1_GIBZE (Q4IAK7) Helicase SWR1 (EC 3.6.1.-) 30 4.3
15MYH9_CHICK (P14105) Myosin-9 (Myosin heavy chain, nonmuscle IIa)... 29 7.3
16CHD1_HUMAN (O14646) Chromodomain-helicase-DNA-binding protein 1 ... 29 7.3
17CHD1_MOUSE (P40201) Chromodomain-helicase-DNA-binding protein 1 ... 29 7.3
18MYH9_HUMAN (P35579) Myosin-9 (Myosin heavy chain, nonmuscle IIa)... 28 9.6

>IF3A_MAIZE (Q9XHR2) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (Eukaryotic translation initiation factor
           3 large subunit) (eIF3a)
          Length = 962

 Score =  225 bits (574), Expect = 4e-59
 Identities = 112/119 (94%), Positives = 118/119 (99%)
 Frame = +1

Query: 121 MATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL 300
           MATFAKPENALKRAEELIHVGQKQ+ALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL
Sbjct: 1   MATFAKPENALKRAEELIHVGQKQSALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL 60

Query: 301 RKGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALDVEDL 477
           RKGRFAKDGLIQYRIVCQQVNVSSLE+VIKHFMQLSNEKAE+A++Q +ALEDALDVEDL
Sbjct: 61  RKGRFAKDGLIQYRIVCQQVNVSSLEDVIKHFMQLSNEKAEQAKSQVEALEDALDVEDL 119



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>IF3A_TOBAC (Q40554) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (Eukaryotic translation initiation factor
           3 large subunit) (eIF3a) (PNLA-35)
          Length = 958

 Score =  206 bits (523), Expect = 3e-53
 Identities = 102/119 (85%), Positives = 112/119 (94%)
 Frame = +1

Query: 121 MATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL 300
           MATFAKPENALKRAEELI VGQKQ ALQALHDLITS+RYR+WQK LE+IM KYVELCVD+
Sbjct: 1   MATFAKPENALKRAEELITVGQKQEALQALHDLITSRRYRAWQKTLERIMFKYVELCVDM 60

Query: 301 RKGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALDVEDL 477
           R+GRFAKDGLIQYRIVCQQVN++SLEEVIKHFM L+ E+AE ARNQAQALE+ALDVEDL
Sbjct: 61  RRGRFAKDGLIQYRIVCQQVNINSLEEVIKHFMHLATERAELARNQAQALEEALDVEDL 119



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>IF3A_ARATH (Q9LD55) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (Eukaryotic translation initiation factor
           3 large subunit) (eIF3a) (p114)
          Length = 987

 Score =  203 bits (517), Expect = 2e-52
 Identities = 99/119 (83%), Positives = 113/119 (94%)
 Frame = +1

Query: 121 MATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDL 300
           MA FAKPENALKRA+ELI+VGQKQ ALQALHDLITSKRYR+WQKPLEKIM KY++LCVDL
Sbjct: 1   MANFAKPENALKRADELINVGQKQDALQALHDLITSKRYRAWQKPLEKIMFKYLDLCVDL 60

Query: 301 RKGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALDVEDL 477
           ++GRFAKDGLIQYRIVCQQVNVSSLEEVIKHF+ L+ +KAE+AR+QA ALE+ALDV+DL
Sbjct: 61  KRGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFLHLATDKAEQARSQADALEEALDVDDL 119



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>IF3A_MOUSE (P23116) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (eIF3 p167) (eIF3 p180) (eIF3 p185)
           (eIF3a) (p162 protein) (Centrosomin)
          Length = 1344

 Score =  137 bits (344), Expect = 2e-32
 Identities = 64/118 (54%), Positives = 91/118 (77%)
 Frame = +1

Query: 124 ATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLR 303
           A F +PENALKRA E + VG+KQ AL  L+D++ SK++R+WQK  E IM+KY+ELCVDLR
Sbjct: 3   AYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLR 62

Query: 304 KGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALDVEDL 477
           K   AK+GL QY+ +CQQVN+ SLE+V++ +++L+ EK E A+ ++Q +   LD+EDL
Sbjct: 63  KSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKLAEEKTEAAKEESQQM--VLDIEDL 118



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>IF3A_HUMAN (Q14152) Eukaryotic translation initiation factor 3 subunit 10|
           (eIF-3 theta) (eIF3 p167) (eIF3 p180) (eIF3 p185)
           (eIF3a)
          Length = 1382

 Score =  136 bits (342), Expect = 3e-32
 Identities = 63/118 (53%), Positives = 91/118 (77%)
 Frame = +1

Query: 124 ATFAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLR 303
           A F +PENALKRA E + VG+KQ AL  L+D++ SK++R+WQK  E IM+KY+ELCVDLR
Sbjct: 3   AYFQRPENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLELCVDLR 62

Query: 304 KGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALDVEDL 477
           K   AK+GL QY+ +CQQVN+ SLE+V++ +++++ EK E A+ ++Q +   LD+EDL
Sbjct: 63  KSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEESQQM--VLDIEDL 118



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>IF3A_CAEEL (P34339) Probable eukaryotic translation initiation factor 3|
           subunit 10 (eIF-3 theta) (eIF3a) (Egg-laying defective
           protein 45)
          Length = 1076

 Score =  119 bits (297), Expect = 5e-27
 Identities = 58/116 (50%), Positives = 83/116 (71%)
 Frame = +1

Query: 130 FAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLRKG 309
           F KPE ALKRAEELI VG++  AL  LHD I ++R++ W    E+IM+K++ELCVDL+K 
Sbjct: 6   FQKPEAALKRAEELIQVGKESDALDTLHDTIKARRHKQWTTVHEQIMIKHMELCVDLKKQ 65

Query: 310 RFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALDVEDL 477
             AKD L QY+ + QQ+NV SLE V+ HF++L+ ++ E+A  Q Q++E   ++ DL
Sbjct: 66  HLAKDALFQYKALTQQINVKSLETVVVHFLKLAEQRTEDA--QKQSIEKVEEIGDL 119



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>IF3A_SCHPO (O74760) Probable eukaryotic translation initiation factor 3 110|
           kDa subunit (eIF3 p110) (Translation initiation factor
           eIF3, p110 subunit) (eIF3a)
          Length = 932

 Score =  103 bits (256), Expect = 3e-22
 Identities = 50/106 (47%), Positives = 77/106 (72%), Gaps = 1/106 (0%)
 Frame = +1

Query: 136 KPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQK-PLEKIMMKYVELCVDLRKGR 312
           KPEN L+ A+ELI + Q  +ALQ+LH+ I  KR R+ Q   LE IMM+++ELCV LRKG+
Sbjct: 7   KPENVLRLADELIALDQHSSALQSLHETIVLKRSRNAQGFSLEPIMMRFIELCVHLRKGK 66

Query: 313 FAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQAL 450
            AK+GL  Y+   Q  +V+++E V+KHF++L+N++ +EA+ +A  +
Sbjct: 67  IAKEGLYTYKNAVQNTSVTAIENVVKHFIELANKRVQEAQEKADKI 112



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>IF3A_YEAST (P38249) Eukaryotic translation initiation factor 3 110 kDa subunit|
           (eIF3 p110) (Translation initiation factor eIF3, p110
           subunit) (eIF3a)
          Length = 964

 Score = 87.8 bits (216), Expect = 1e-17
 Identities = 50/118 (42%), Positives = 76/118 (64%), Gaps = 4/118 (3%)
 Frame = +1

Query: 136 KPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKP--LEKIMMKYVELCVDLRKG 309
           +PENA+KRA+ELI VG+KQAALQ+LHD IT++R R W  P  +E ++ K++E+ V+L+KG
Sbjct: 7   RPENAIKRADELISVGEKQAALQSLHDFITARRIR-WATPSTVEPVVFKFLEIGVELKKG 65

Query: 310 RFAKDGLIQYRIVCQ--QVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALDVEDL 477
           +  KDGL QY+ + Q     + S+  V + F+ L   K    + +A  L+     +DL
Sbjct: 66  KLLKDGLHQYKKLIQGSTEGLVSVGAVARKFIDLVESKIASEQTRADELQKQEIDDDL 123



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>RGP1_XENLA (O13066) Ran GTPase-activating protein 1|
          Length = 580

 Score = 31.6 bits (70), Expect = 1.1
 Identities = 15/41 (36%), Positives = 29/41 (70%)
 Frame = +1

Query: 343 IVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALD 465
           ++ QQVNV+  E+V++ F+Q+S+   E+   +A A+E+ +D
Sbjct: 449 MIAQQVNVADTEKVVQAFIQVSSVYREDGEIKA-AVEETID 488



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>BPAEB_HUMAN (Q8WXK8) Bullous pemphigoid antigen 1, isoform 7 (Bullous pemphigoid|
            antigen) (BPA) (Hemidesmosomal plaque protein) (Dystonia
            musculorum protein) (Dystonin) (Fragment)
          Length = 3060

 Score = 31.6 bits (70), Expect = 1.1
 Identities = 20/66 (30%), Positives = 32/66 (48%)
 Frame = +1

Query: 190  QAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLRKGRFAKDGLIQYRIVCQQVNVS 369
            QA L+ L D  T  R    Q  +  ++   +  C DL+  +    GLI  +I+CQ   +S
Sbjct: 1245 QAVLRGLIDYDTGIRLLETQLMISGLISPELRKCFDLKDAK--SHGLIDEQILCQLKELS 1302

Query: 370  SLEEVI 387
              +E+I
Sbjct: 1303 KAKEII 1308



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>IF2A_THEAC (Q9HIX3) Translation initiation factor 2 alpha subunit|
           (eIF-2-alpha) (aIF2-alpha)
          Length = 254

 Score = 30.4 bits (67), Expect = 2.5
 Identities = 13/40 (32%), Positives = 25/40 (62%)
 Frame = +1

Query: 325 GLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQ 444
           G  +YRIV +  +    EE++K  +Q+ NEKA++ + + +
Sbjct: 211 GAPRYRIVVKDKDYKKAEEILKKVVQIVNEKAKKLQVEVE 250



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>Y1002_RHIME (Q52969) Hypothetical sensor-like histidine kinase R01002 (EC|
           2.7.13.3)
          Length = 525

 Score = 30.4 bits (67), Expect = 2.5
 Identities = 29/103 (28%), Positives = 43/103 (41%), Gaps = 1/103 (0%)
 Frame = +1

Query: 166 ELIHVGQKQAALQALHDL-ITSKRYRSWQKPLEKIMMKYVELCVDLRKGRFAKDGLIQYR 342
           E IHV  + A L+A+  L + S+R                   VD+R  R + DG     
Sbjct: 149 EQIHVSDRIAFLRAIDSLRLDSERS-----------------AVDIRLERTSADGPQFAH 191

Query: 343 IVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALDVE 471
           I C+   +   E  +   +  S + +EEAR QA+A   A   E
Sbjct: 192 IYCEMTPLRDAEGNLLAIVAQSRDVSEEARLQAEAAAKAAHAE 234



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>RAD50_PYRAB (Q9UZC8) DNA double-strand break repair rad50 ATPase|
          Length = 880

 Score = 30.0 bits (66), Expect = 3.3
 Identities = 25/103 (24%), Positives = 45/103 (43%), Gaps = 6/103 (5%)
 Frame = +1

Query: 133 AKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIMMKYVELCVDLRKGR 312
           A  +   K+  E+  +  K   ++        ++ R   K LE I  K  EL + + K +
Sbjct: 201 ANEDELTKKLSEINEISSKLPPIRG-----ELEKVRENVKELESIKGKISELKIQVEKLK 255

Query: 313 FAKDGL------IQYRIVCQQVNVSSLEEVIKHFMQLSNEKAE 423
             K GL      I+  I  ++  +S LEE++K   +L  ++ E
Sbjct: 256 GRKKGLEEKIVQIERSIEEKKAKISELEEIVKDIPKLQEKEKE 298



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>SWR1_GIBZE (Q4IAK7) Helicase SWR1 (EC 3.6.1.-)|
          Length = 1691

 Score = 29.6 bits (65), Expect = 4.3
 Identities = 23/95 (24%), Positives = 42/95 (44%), Gaps = 3/95 (3%)
 Frame = +1

Query: 130  FAKPENALKRAEELIHVGQKQAALQALHDLITSKRYRSWQKPLEKIM---MKYVELCVDL 300
            FAKPE+ +  +       + +A +  LH ++     R  +  +EK M    ++VE C   
Sbjct: 1005 FAKPESQILESGREQMDDEARAIISKLHKVLRPYLLRRLKADVEKQMPAKYEHVEFCRLS 1064

Query: 301  RKGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQL 405
            ++ R   DG +      + +N  +   +I   MQL
Sbjct: 1065 KRQRELYDGFLSRTDTKETLNSGNYLSIINCLMQL 1099



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>MYH9_CHICK (P14105) Myosin-9 (Myosin heavy chain, nonmuscle IIa) (Nonmuscle|
            myosin heavy chain IIa) (NMMHC II-a) (NMMHC-IIA)
            (Cellular myosin heavy chain, type A) (Nonmuscle myosin
            heavy chain-A) (NMMHC-A)
          Length = 1959

 Score = 28.9 bits (63), Expect = 7.3
 Identities = 32/123 (26%), Positives = 56/123 (45%), Gaps = 3/123 (2%)
 Frame = +1

Query: 115  PAMATFAKPENALKRAEELIHVGQKQ-AALQALHDLITSKRYRSWQK-PLEKIMMKYVEL 288
            P +    + E  + + EELI V +KQ AA   L ++ T +     +K  L++ +    EL
Sbjct: 836  PLLQVSRQEEEMMAKEEELIKVKEKQLAAENRLSEMETFQAQLMAEKMQLQEQLQAEAEL 895

Query: 289  CVDLRKGRFAKDGLIQ-YRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDALD 465
            C +  + R       Q    +C  +  + +EE  +    L  EK +  +N  Q LE+ L+
Sbjct: 896  CAEAEEIRARLTAKKQELEEICHDLE-ARVEEEEERCQHLQAEKKKMQQN-IQELEEQLE 953

Query: 466  VED 474
             E+
Sbjct: 954  EEE 956



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>CHD1_HUMAN (O14646) Chromodomain-helicase-DNA-binding protein 1 (EC 3.6.1.-)|
            (ATP-dependent helicase CHD1) (CHD-1)
          Length = 1709

 Score = 28.9 bits (63), Expect = 7.3
 Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 5/73 (6%)
 Frame = +1

Query: 103  RPLAPAMATFAKPENALKRAEELIHVGQKQAAL-----QALHDLITSKRYRSWQKPLEKI 267
            RP+  A+    +PE  L   E+L H  Q    +     + L +    ++ + W+K L   
Sbjct: 1422 RPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYTNPEQIKQWRKNLWIF 1481

Query: 268  MMKYVELCVDLRK 306
            + K+ E   D RK
Sbjct: 1482 VSKFTEF--DARK 1492



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>CHD1_MOUSE (P40201) Chromodomain-helicase-DNA-binding protein 1 (EC 3.6.1.-)|
            (ATP-dependent helicase CHD1) (CHD-1)
          Length = 1711

 Score = 28.9 bits (63), Expect = 7.3
 Identities = 28/125 (22%), Positives = 51/125 (40%), Gaps = 6/125 (4%)
 Frame = +1

Query: 103  RPLAPAMATFAKPENALKRAEELIHVGQKQAAL-----QALHDLITSKRYRSWQKPLEKI 267
            RP+  A+    +PE  L   E+L H  Q    +     + L +    ++ + W+K L   
Sbjct: 1422 RPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYSNPEQIKQWRKNLWIF 1481

Query: 268  MMKYVELCVDLRKGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSN-EKAEEARNQAQ 444
            + K+ E   D RK        I+ R   QQ +  +      H ++  + E+ +E  N   
Sbjct: 1482 VSKFTEF--DARKLHKLYKHAIKKRQESQQNSDQNSNVATTHVIRNPDMERLKENTNHDD 1539

Query: 445  ALEDA 459
            +  D+
Sbjct: 1540 SSRDS 1544



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>MYH9_HUMAN (P35579) Myosin-9 (Myosin heavy chain, nonmuscle IIa) (Nonmuscle|
            myosin heavy chain IIa) (NMMHC II-a) (NMMHC-IIA)
            (Cellular myosin heavy chain, type A) (Nonmuscle myosin
            heavy chain-A) (NMMHC-A)
          Length = 1959

 Score = 28.5 bits (62), Expect = 9.6
 Identities = 33/125 (26%), Positives = 59/125 (47%), Gaps = 5/125 (4%)
 Frame = +1

Query: 115  PAMATFAKPENALKRAEELIHVGQKQ-AALQALHDLITSKRYRSWQK-PLEKIMMKYVEL 288
            P +    + E  + + EEL+ V +KQ AA   L ++ T +     +K  L++ +    EL
Sbjct: 835  PLLQVSRQEEEMMAKEEELVKVREKQLAAENRLTEMETLQSQLMAEKLQLQEQLQAETEL 894

Query: 289  CV---DLRKGRFAKDGLIQYRIVCQQVNVSSLEEVIKHFMQLSNEKAEEARNQAQALEDA 459
            C    +LR    AK   ++   +C  +  + +EE  +    L  EK +  +N  Q LE+ 
Sbjct: 895  CAEAEELRARLTAKKQELEE--ICHDLE-ARVEEEEERCQHLQAEKKKMQQN-IQELEEQ 950

Query: 460  LDVED 474
            L+ E+
Sbjct: 951  LEEEE 955


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,057,840
Number of Sequences: 219361
Number of extensions: 935059
Number of successful extensions: 2945
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 2875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2943
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3246866728
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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