ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl18g09
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane pro... 189 1e-48
2AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane pro... 147 8e-36
3AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane pr... 143 1e-34
4HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.... 33 0.16
5HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.... 33 0.16
6HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.... 27 8.8

>AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase)
          Length = 762

 Score =  189 bits (481), Expect = 1e-48
 Identities = 95/104 (91%), Positives = 95/104 (91%)
 Frame = +3

Query: 99  MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXKNGYGDYLIEEEE 278
           MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTP         KNGYGDYLIEEEE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAAAGAKNGYGDYLIEEEE 60

Query: 279 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAII 410
           GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAII
Sbjct: 61  GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAII 104



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>AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase) (Vacuolar
           H(+)-pyrophosphatase)
          Length = 764

 Score =  147 bits (370), Expect = 8e-36
 Identities = 74/104 (71%), Positives = 83/104 (79%), Gaps = 1/104 (0%)
 Frame = +3

Query: 102 AILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXX-XXXXKNGYGDYLIEEEE 278
           AIL +LGTEILIPVC VIGI FA+ QW +VSKVK++           KNGY DYLIEEEE
Sbjct: 3   AILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPNAAAKNGYNDYLIEEEE 62

Query: 279 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAII 410
           G+NDHNVVVKCAEIQ AISEGATSFLFT Y+YVG+FMV FA +I
Sbjct: 63  GINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILI 106



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>AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane proton pump 1|
           (EC 3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase 1) (H(+)-PPase 1) (Vacuolar proton
           pyrophosphatase 1) (Vacuolar proton pyrophosphatase 3)
          Length = 770

 Score =  143 bits (360), Expect = 1e-34
 Identities = 70/111 (63%), Positives = 86/111 (77%), Gaps = 4/111 (3%)
 Frame = +3

Query: 90  LTAMAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXX----KNGYGD 257
           + A A+L EL TEIL+P+C VIGI F++ QW++VS+VK+T              KNGYGD
Sbjct: 1   MVAPALLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGD 60

Query: 258 YLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAII 410
           YLIEEEEG+ND +VV KCAEIQTAISEGATSFLFT Y+YVG+FM+ FAA+I
Sbjct: 61  YLIEEEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVI 111



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>HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 33.1 bits (74), Expect = 0.16
 Identities = 15/35 (42%), Positives = 22/35 (62%)
 Frame = +3

Query: 306 KCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAII 410
           K  EI +AISEGA +FL   Y+ + +F+   A +I
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLI 76



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>HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 33.1 bits (74), Expect = 0.16
 Identities = 15/35 (42%), Positives = 22/35 (62%)
 Frame = +3

Query: 306 KCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAII 410
           K  EI +AISEGA +FL   Y+ + +F+   A +I
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLI 76



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>HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 673

 Score = 27.3 bits (59), Expect = 8.8
 Identities = 13/32 (40%), Positives = 20/32 (62%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAII 410
           EI   I +GA +FL T Y+Y+  F+V+   I+
Sbjct: 38  EIAGHIHDGAMAFLKTEYKYLTGFIVIVTVIL 69


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,768,876
Number of Sequences: 219361
Number of extensions: 317750
Number of successful extensions: 1085
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1072
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1079
length of database: 80,573,946
effective HSP length: 112
effective length of database: 56,005,514
effective search space used: 1344132336
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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