ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl18a01
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (E... 135 2e-32
2EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (E... 134 4e-32
3MANBA_CAPHI (Q95327) Beta-mannosidase precursor (EC 3.2.1.25) (L... 32 0.51
4MANBA_BOVIN (Q29444) Beta-mannosidase precursor (EC 3.2.1.25) (L... 31 0.86
5COAE_RHOPA (Q6ND09) Dephospho-CoA kinase (EC 2.7.1.24) (Dephosph... 28 5.6
6POLN_SLDV (Q8QL53) Nonstructural polyprotein (Polyprotein nsP123... 27 9.5
7POLN_SPDV (Q8JJX1) Nonstructural polyprotein (Polyprotein nsP123... 27 9.5

>EBM_LILLO (Q5H7P5) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
           (Endo-beta-mannosidase) [Contains: Mannosylglycoprotein
           endo-beta-mannosidase 31 kDa subunit;
           Mannosylglycoprotein endo-beta-mannosidase 28 kDa
           subunit; Mannosylglycoprotein end
          Length = 952

 Score =  135 bits (341), Expect = 2e-32
 Identities = 63/83 (75%), Positives = 71/83 (85%)
 Frame = +2

Query: 98  GKLVLDTGWLAARFTEVSLTGVDLTTTHPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDP 277
           GK VLD+GWLAAR TE+ LTGV LTTT PP  + +APW+ A VPGTVLGTLLKNKL+PDP
Sbjct: 1   GKQVLDSGWLAARSTELELTGVQLTTTRPPSGT-SAPWIEAVVPGTVLGTLLKNKLVPDP 59

Query: 278 FYGLNNEAIIDIADSGRDYYTFW 346
           FYGLNNE I+DI DSGR+YYTFW
Sbjct: 60  FYGLNNEGILDIYDSGREYYTFW 82



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>EBM_ARATH (Q75W54) Mannosylglycoprotein endo-beta-mannosidase (EC 3.2.1.152)|
           (Endo-beta-mannosidase) (AtEBM) [Contains:
           Mannosylglycoprotein endo-beta-mannosidase 31 kDa
           subunit; Mannosylglycoprotein endo-beta-mannosidase 28
           kDa subunit; Mannosylglycopro
          Length = 943

 Score =  134 bits (338), Expect = 4e-32
 Identities = 63/84 (75%), Positives = 69/84 (82%)
 Frame = +2

Query: 95  VGKLVLDTGWLAARFTEVSLTGVDLTTTHPPDASVAAPWMHAAVPGTVLGTLLKNKLIPD 274
           +GK VLD GW+AAR TEV + GV LTTT+PP  S  + WM AAVPGTVLGTL+KNK IPD
Sbjct: 3   IGKTVLDFGWIAARSTEVDVNGVQLTTTNPPAISSESRWMEAAVPGTVLGTLVKNKAIPD 62

Query: 275 PFYGLNNEAIIDIADSGRDYYTFW 346
           PFYGL NEAI DIADSGRDYYTFW
Sbjct: 63  PFYGLENEAITDIADSGRDYYTFW 86



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>MANBA_CAPHI (Q95327) Beta-mannosidase precursor (EC 3.2.1.25) (Lysosomal beta A|
           mannosidase) (Mannanase) (Mannase)
          Length = 879

 Score = 31.6 bits (70), Expect = 0.51
 Identities = 16/39 (41%), Positives = 21/39 (53%)
 Frame = +2

Query: 179 HPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDPFYGLNN 295
           H  + S+  P   AAVPG V   L   ++I DP+Y  NN
Sbjct: 32  HNGNGSLQLP---AAVPGCVHSALFNKRIIKDPYYRFNN 67



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>MANBA_BOVIN (Q29444) Beta-mannosidase precursor (EC 3.2.1.25) (Lysosomal beta A|
           mannosidase) (Mannanase) (Mannase)
          Length = 879

 Score = 30.8 bits (68), Expect = 0.86
 Identities = 19/56 (33%), Positives = 26/56 (46%)
 Frame = +2

Query: 128 AARFTEVSLTGVDLTTTHPPDASVAAPWMHAAVPGTVLGTLLKNKLIPDPFYGLNN 295
           A +   +SL G      H  + S+  P   A VPG V   L   ++I DP+Y  NN
Sbjct: 17  ATKVVSISLRGN--WKIHSGNGSLQLP---ATVPGCVHSALFNKRIIKDPYYRFNN 67



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>COAE_RHOPA (Q6ND09) Dephospho-CoA kinase (EC 2.7.1.24) (Dephosphocoenzyme A|
           kinase)
          Length = 199

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 21/66 (31%), Positives = 28/66 (42%), Gaps = 6/66 (9%)
 Frame = +2

Query: 98  GKLVLDTGWLAARFTEVSLTGVDL-TTTHPPDASVAAPWMHAAVPGTVLG-----TLLKN 259
           G + +     A  F E  +   D   T H    + A P + AA PGT LG      LL  
Sbjct: 8   GSIGMGKSTTAKLFAEAGVPVYDADATVHKIYENEAVPAIEAAFPGTTLGGKVDRALLSA 67

Query: 260 KLIPDP 277
           K++ DP
Sbjct: 68  KVVHDP 73



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>POLN_SLDV (Q8QL53) Nonstructural polyprotein (Polyprotein nsP1234) (P1234)|
            [Contains: P123; mRNA capping enzyme nsP1 (EC 2.1.1.-)
            (EC 2.7.7.-) (Nonstructural protein 1);
            Protease/triphosphatase/NTPase/helicase nsP2 (EC
            3.4.22.-) (EC 3.1.3.33) (EC 3.6.1.1
          Length = 2593

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = -3

Query: 225  TAACIHGAATEASGGWVVVRSTPVRDTSVKRA 130
            T+AC HG    A GG  +V++  + D   +RA
Sbjct: 1306 TSACKHGVERTAKGGVFIVKAYGMADRRTERA 1337



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>POLN_SPDV (Q8JJX1) Nonstructural polyprotein (Polyprotein nsP1234) (P1234)|
            [Contains: P123; mRNA capping enzyme nsP1 (EC 2.1.1.-)
            (EC 2.7.7.-) (Nonstructural protein 1);
            Protease/triphosphatase/NTPase/helicase nsP2 (EC
            3.4.22.-) (EC 3.1.3.33) (EC 3.6.1.1
          Length = 2601

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = -3

Query: 225  TAACIHGAATEASGGWVVVRSTPVRDTSVKRA 130
            T+AC HG    A GG  +V++  + D   +RA
Sbjct: 1307 TSACKHGVERTAKGGVFIVKAYGMADRRTERA 1338


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,149,389
Number of Sequences: 219361
Number of extensions: 458939
Number of successful extensions: 1418
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1389
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1417
length of database: 80,573,946
effective HSP length: 91
effective length of database: 60,612,095
effective search space used: 1454690280
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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