ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl13g05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane pro... 177 7e-45
2AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane pro... 138 4e-33
3AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane pr... 132 2e-31
4HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.... 30 1.8
5HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.... 30 1.8

>AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase)
          Length = 762

 Score =  177 bits (448), Expect = 7e-45
 Identities = 88/99 (88%), Positives = 89/99 (89%)
 Frame = +2

Query: 101 MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXENGYGDYLIEEEE 280
           MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTP         +NGYGDYLIEEEE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAAAGAKNGYGDYLIEEEE 60

Query: 281 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMGV 397
           GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFM V
Sbjct: 61  GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVV 99



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>AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase) (Vacuolar
           H(+)-pyrophosphatase)
          Length = 764

 Score =  138 bits (347), Expect = 4e-33
 Identities = 69/97 (71%), Positives = 78/97 (80%), Gaps = 1/97 (1%)
 Frame = +2

Query: 104 AILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXX-XXXXENGYGDYLIEEEE 280
           AIL +LGTEILIPVC VIGI FA+ QW +VSKVK++           +NGY DYLIEEEE
Sbjct: 3   AILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPNAAAKNGYNDYLIEEEE 62

Query: 281 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFM 391
           G+NDHNVVVKCAEIQ AISEGATSFLFT Y+YVG+FM
Sbjct: 63  GINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFM 99



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>AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane proton pump 1|
           (EC 3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase 1) (H(+)-PPase 1) (Vacuolar proton
           pyrophosphatase 1) (Vacuolar proton pyrophosphatase 3)
          Length = 770

 Score =  132 bits (333), Expect = 2e-31
 Identities = 65/104 (62%), Positives = 80/104 (76%), Gaps = 4/104 (3%)
 Frame = +2

Query: 92  LTAMAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXX----ENGYGD 259
           + A A+L EL TEIL+P+C VIGI F++ QW++VS+VK+T              +NGYGD
Sbjct: 1   MVAPALLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGD 60

Query: 260 YLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFM 391
           YLIEEEEG+ND +VV KCAEIQTAISEGATSFLFT Y+YVG+FM
Sbjct: 61  YLIEEEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFM 104



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>HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 29.6 bits (65), Expect = 1.8
 Identities = 13/28 (46%), Positives = 19/28 (67%)
 Frame = +2

Query: 308 KCAEIQTAISEGATSFLFTMYQYVGMFM 391
           K  EI +AISEGA +FL   Y+ + +F+
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFI 69



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>HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 29.6 bits (65), Expect = 1.8
 Identities = 13/28 (46%), Positives = 19/28 (67%)
 Frame = +2

Query: 308 KCAEIQTAISEGATSFLFTMYQYVGMFM 391
           K  EI +AISEGA +FL   Y+ + +F+
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFI 69


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,615,454
Number of Sequences: 219361
Number of extensions: 326459
Number of successful extensions: 1101
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 1091
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1099
length of database: 80,573,946
effective HSP length: 107
effective length of database: 57,102,319
effective search space used: 1370455656
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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