ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl09h08
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1ASPG2_ARATH (Q8GXG1) Probable L-asparaginase 2 precursor (EC 3.5... 93 2e-19
2ASPG1_ARATH (P50287) L-asparaginase 1 precursor (EC 3.5.1.1) (L-... 62 5e-10
3ASPG_LUPLU (Q9ZSD6) L-asparaginase precursor (EC 3.5.1.1) (L-asp... 61 6e-10
4ASPG_LUPAN (P30364) L-asparaginase precursor (EC 3.5.1.1) (L-asp... 60 1e-09
5ASPG_LUPAL (P50288) L-asparaginase precursor (EC 3.5.1.1) (L-asp... 60 1e-09
6ASPG_LUPAR (P30362) L-asparaginase precursor (EC 3.5.1.1) (L-asp... 44 1e-04
7ASGX_ECOLI (P37595) Putative L-asparaginase precursor (EC 3.5.1.... 43 2e-04
8TASP1_MOUSE (Q8R1G1) Threonine aspartase 1 (EC 3.4.25.-) (Taspas... 39 0.002
9TASP1_HUMAN (Q9H6P5) Threonine aspartase 1 (EC 3.4.25.-) (Taspas... 39 0.002
10CCA_XYLFT (Q87DS9) Multifunctional CCA protein [Includes: CCA-ad... 32 0.30
11CCA_XYLFA (Q9PDL7) Multifunctional CCA protein [Includes: CCA-ad... 31 0.66
12BRN3_CHICK (Q91998) Brain-specific homeobox/POU domain protein 3... 30 1.9
13ASPG4_ARATH (O65268) Putative L-asparaginase 4 precursor (EC 3.5... 30 1.9
14NADB1_RALSO (Q8XWM7) L-aspartate oxidase 1 (EC 1.4.3.16) (LASPO ... 28 5.6
15SFSA_AERPE (Q9YEX9) Sugar fermentation stimulation protein homolog 28 5.6
16AMPM_TREPA (O83814) Methionine aminopeptidase (EC 3.4.11.18) (MA... 28 7.3
17ENV_HV1KB (P31819) Envelope polyprotein GP160 precursor [Contain... 28 7.3
18DCOR_ECOLI (P21169) Ornithine decarboxylase, constitutive (EC 4.... 28 7.3
19MAF_AVIS4 (P23091) Transforming protein Maf 27 9.5
20PQQF_KLEPN (P27508) Coenzyme PQQ synthesis protein F (EC 3.4.99.... 27 9.5

>ASPG2_ARATH (Q8GXG1) Probable L-asparaginase 2 precursor (EC 3.5.1.1)|
           (L-asparagine amidohydrolase 2) [Contains:
           L-asparaginase 2 alpha subunit; L-asparaginase 2 beta
           subunit]
          Length = 325

 Score = 92.8 bits (229), Expect = 2e-19
 Identities = 41/62 (66%), Positives = 51/62 (82%)
 Frame = +2

Query: 158 MARWAIAIHGGAGVDPNLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELET 337
           M  WAIA+HGGAG+DPNLP  RQEEAK++L RCL +G+  LR+  +A+DVVE V+RELET
Sbjct: 1   MGGWAIAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELET 60

Query: 338 DP 343
           DP
Sbjct: 61  DP 62



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>ASPG1_ARATH (P50287) L-asparaginase 1 precursor (EC 3.5.1.1) (L-asparagine|
           amidohydrolase 1) [Contains: L-asparaginase 1 alpha
           subunit; L-asparaginase 1 beta subunit]
          Length = 315

 Score = 61.6 bits (148), Expect = 5e-10
 Identities = 31/63 (49%), Positives = 40/63 (63%), Gaps = 1/63 (1%)
 Frame = +2

Query: 158 MARWAIAIHGGAGVDP-NLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELE 334
           M  WAIA+HGGAG  P +LP+ R+   +  L  CL +G+  L++G   LDV E VVRELE
Sbjct: 1   MVGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELE 60

Query: 335 TDP 343
             P
Sbjct: 61  NHP 63



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>ASPG_LUPLU (Q9ZSD6) L-asparaginase precursor (EC 3.5.1.1) (L-asparagine|
           amidohydrolase) [Contains: L-asparaginase alpha subunit;
           L-asparaginase beta subunit]
          Length = 325

 Score = 61.2 bits (147), Expect = 6e-10
 Identities = 32/60 (53%), Positives = 41/60 (68%), Gaps = 1/60 (1%)
 Frame = +2

Query: 158 MARWAIAIHGGAGVDP-NLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELE 334
           M  W+IA+HGGAG  P +LP  R++  +  L  CLQ+GV+ L+A    LDVVE VVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELE 60



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>ASPG_LUPAN (P30364) L-asparaginase precursor (EC 3.5.1.1) (L-asparagine|
           amidohydrolase) [Contains: L-asparaginase alpha subunit;
           L-asparaginase beta subunit]
          Length = 325

 Score = 60.5 bits (145), Expect = 1e-09
 Identities = 31/60 (51%), Positives = 41/60 (68%), Gaps = 1/60 (1%)
 Frame = +2

Query: 158 MARWAIAIHGGAGVDP-NLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELE 334
           M  W+IA+HGGAG  P +LP  R++  +  L  CLQ+GV+ L++    LDVVE VVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELE 60



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>ASPG_LUPAL (P50288) L-asparaginase precursor (EC 3.5.1.1) (L-asparagine|
           amidohydrolase) [Contains: L-asparaginase alpha subunit;
           L-asparaginase beta subunit]
          Length = 325

 Score = 60.5 bits (145), Expect = 1e-09
 Identities = 31/60 (51%), Positives = 41/60 (68%), Gaps = 1/60 (1%)
 Frame = +2

Query: 158 MARWAIAIHGGAGVDP-NLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELE 334
           M  W+IA+HGGAG  P +LP  R++  +  L  CLQ+G++ L+A    LDVVE VVRELE
Sbjct: 1   MGGWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELE 60



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>ASPG_LUPAR (P30362) L-asparaginase precursor (EC 3.5.1.1) (L-asparagine|
           amidohydrolase) [Contains: L-asparaginase alpha subunit;
           L-asparaginase beta subunit] (Fragment)
          Length = 306

 Score = 43.5 bits (101), Expect = 1e-04
 Identities = 21/43 (48%), Positives = 29/43 (67%)
 Frame = +2

Query: 212 PEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELETD 340
           P  R++  +  L  CLQ+GV+ L+A  + LDVVE VVRELE +
Sbjct: 1   PPERRKPREEGLRHCLQIGVEALKARKSPLDVVELVVRELENN 43



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>ASGX_ECOLI (P37595) Putative L-asparaginase precursor (EC 3.5.1.1)|
           (L-asparagine amidohydrolase)
          Length = 321

 Score = 42.7 bits (99), Expect = 2e-04
 Identities = 26/64 (40%), Positives = 35/64 (54%), Gaps = 2/64 (3%)
 Frame = +2

Query: 158 MARWAIAIHGGAGVDPNLPEHRQEEAKRV--LARCLQVGVDLLRAGATALDVVEAVVREL 331
           M +  IAIHGGAG         Q+E + +  L+  ++ G  +L AG +ALDVV   VR L
Sbjct: 1   MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGESALDVVTEAVRLL 60

Query: 332 ETDP 343
           E  P
Sbjct: 61  EECP 64



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>TASP1_MOUSE (Q8R1G1) Threonine aspartase 1 (EC 3.4.25.-) (Taspase-1) [Contains:|
           Threonine aspartase subunit alpha; Threonine aspartase
           subunit beta]
          Length = 420

 Score = 39.3 bits (90), Expect = 0.002
 Identities = 22/57 (38%), Positives = 30/57 (52%)
 Frame = +2

Query: 173 IAIHGGAGVDPNLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELETDP 343
           + +H GAG      E + +E K V  R  Q  ++ L+AGA A D V A + ELE  P
Sbjct: 44  VLVHAGAGYHS---ESKAKEYKHVCKRACQKAIEKLQAGALATDAVAAALVELEDSP 97



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>TASP1_HUMAN (Q9H6P5) Threonine aspartase 1 (EC 3.4.25.-) (Taspase-1) [Contains:|
           Threonine aspartase subunit alpha; Threonine aspartase
           subunit beta]
          Length = 420

 Score = 39.3 bits (90), Expect = 0.002
 Identities = 22/57 (38%), Positives = 30/57 (52%)
 Frame = +2

Query: 173 IAIHGGAGVDPNLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELETDP 343
           + +H GAG      E + +E K V  R  Q  ++ L+AGA A D V A + ELE  P
Sbjct: 44  VLVHAGAGYHS---ESKAKEYKHVCKRACQKAIEKLQAGALATDAVTAALVELEDSP 97



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>CCA_XYLFT (Q87DS9) Multifunctional CCA protein [Includes: CCA-adding enzyme|
           (EC 2.7.7.25) (EC 2.7.7.21) (tRNA
           nucleotidyltransferase) (tRNA
           adenylyl-/cytidylyl-transferase) (tRNA
           CCA-pyrophosphorylase) (tRNA-NT); 2'-nucleotidase (EC
           3.1.3.-); 2',3'-cycli
          Length = 416

 Score = 32.3 bits (72), Expect = 0.30
 Identities = 19/64 (29%), Positives = 29/64 (45%)
 Frame = -2

Query: 337 RLQLPHHGLHHVQRGGAGAQQVDAHLQAPGQHPLGLLLAVLRQVRVHARAAVDGNGPARH 158
           R   PHH +H  QRG A  QQ+   L+ P  +    L+A    + VH  + +  N     
Sbjct: 266 RATWPHHPMHE-QRGMAPTQQLSERLKVPRNYQQLALIACREHLNVHRLSKLHDNTVYEL 324

Query: 157 VRAC 146
           ++ C
Sbjct: 325 LQRC 328



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>CCA_XYLFA (Q9PDL7) Multifunctional CCA protein [Includes: CCA-adding enzyme|
           (EC 2.7.7.25) (EC 2.7.7.21) (tRNA
           nucleotidyltransferase) (tRNA
           adenylyl-/cytidylyl-transferase) (tRNA
           CCA-pyrophosphorylase) (tRNA-NT); 2'-nucleotidase (EC
           3.1.3.-); 2',3'-cycli
          Length = 416

 Score = 31.2 bits (69), Expect = 0.66
 Identities = 17/47 (36%), Positives = 23/47 (48%)
 Frame = -2

Query: 337 RLQLPHHGLHHVQRGGAGAQQVDAHLQAPGQHPLGLLLAVLRQVRVH 197
           R   PHH +H  QRG A  QQ+   L+ P  +    L+A    + VH
Sbjct: 266 RATWPHHPMHE-QRGMAPTQQLSERLKVPRDYQQLALIACREHLNVH 311



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>BRN3_CHICK (Q91998) Brain-specific homeobox/POU domain protein 3 (Brn-3)|
          Length = 341

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 10/27 (37%), Positives = 15/27 (55%)
 Frame = +1

Query: 94  THRSAFRHLAQHTTTVTYTHAHGALGH 174
           +H SA  H+A H+  +  +H HG   H
Sbjct: 149 SHMSAINHMAHHSQPMNMSHPHGLASH 175



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>ASPG4_ARATH (O65268) Putative L-asparaginase 4 precursor (EC 3.5.1.1)|
           (L-asparagine amidohydrolase 4) [Contains:
           L-asparaginase 4 alpha subunit; L-asparaginase 4 beta
           subunit]
          Length = 407

 Score = 29.6 bits (65), Expect = 1.9
 Identities = 18/60 (30%), Positives = 25/60 (41%)
 Frame = +2

Query: 164 RWAIAIHGGAGVDPNLPEHRQEEAKRVLARCLQVGVDLLRAGATALDVVEAVVRELETDP 343
           R+ +A+H GAG      E       R    CL     L +     +D V A ++ LE DP
Sbjct: 11  RFFVAVHVGAGYHAAANEKALRSVMR--RACLAASTILRQDSGECIDAVSAAIQVLEDDP 68



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>NADB1_RALSO (Q8XWM7) L-aspartate oxidase 1 (EC 1.4.3.16) (LASPO 1) (Quinolinate|
           synthetase B 1)
          Length = 533

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 11/24 (45%), Positives = 16/24 (66%)
 Frame = +2

Query: 212 PEHRQEEAKRVLARCLQVGVDLLR 283
           P   QE    +LARCL++G+D+ R
Sbjct: 309 PAFIQEHFPTILARCLELGIDITR 332



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>SFSA_AERPE (Q9YEX9) Sugar fermentation stimulation protein homolog|
          Length = 240

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 17/46 (36%), Positives = 22/46 (47%), Gaps = 6/46 (13%)
 Frame = +2

Query: 167 WAIAIHG------GAGVDPNLPEHRQEEAKRVLARCLQVGVDLLRA 286
           WA+ + G      G G+ PN P  R  +   VLAR  + GV  L A
Sbjct: 126 WAVEVKGVNLSLDGVGLFPNAPSARAVKHLEVLARLAREGVKPLMA 171



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>AMPM_TREPA (O83814) Methionine aminopeptidase (EC 3.4.11.18) (MAP) (Peptidase|
           M)
          Length = 255

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 14/34 (41%), Positives = 17/34 (50%)
 Frame = +2

Query: 218 HRQEEAKRVLARCLQVGVDLLRAGATALDVVEAV 319
           H + E  RV   CL+ G+   RAGA    V  AV
Sbjct: 116 HERLELLRVTTECLRAGIKACRAGARVRAVSRAV 149



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>ENV_HV1KB (P31819) Envelope polyprotein GP160 precursor [Contains: Exterior|
           membrane glycoprotein (GP120); Transmembrane
           glycoprotein (GP41)]
          Length = 861

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 11/26 (42%), Positives = 14/26 (53%)
 Frame = -3

Query: 258 RHRASTRLASSWRCSGRFGSTPAPPW 181
           R+    +L   W CSG+F  T A PW
Sbjct: 591 RYLKDQQLMGIWGCSGKFICTTAVPW 616



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>DCOR_ECOLI (P21169) Ornithine decarboxylase, constitutive (EC 4.1.1.17)|
          Length = 711

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 10/32 (31%), Positives = 19/32 (59%)
 Frame = +2

Query: 191 AGVDPNLPEHRQEEAKRVLARCLQVGVDLLRA 286
           A +D N   H  E  +R+ A C+++G++  +A
Sbjct: 395 AALDVNAKIHEGESGRRLWAECVEIGIEARKA 426



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>MAF_AVIS4 (P23091) Transforming protein Maf|
          Length = 369

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 12/28 (42%), Positives = 12/28 (42%)
 Frame = -2

Query: 322 HHGLHHVQRGGAGAQQVDAHLQAPGQHP 239
           HH  HH   GG G      H  APG  P
Sbjct: 175 HHHHHHPHHGGGGGGGGHPHGAAPGSAP 202



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>PQQF_KLEPN (P27508) Coenzyme PQQ synthesis protein F (EC 3.4.99.-)|
           (Pyrroloquinoline quinone biosynthesis protein F)
          Length = 761

 Score = 27.3 bits (59), Expect = 9.5
 Identities = 24/88 (27%), Positives = 36/88 (40%), Gaps = 11/88 (12%)
 Frame = -2

Query: 328 LPHHGLHHVQRGGAGAQQVDAHLQAPGQHPLGLLLAVLRQVRVHARAAVDG--NGPARHV 155
           L H G H       G+ Q+   L  PG+ P  +L A+LRQ+ +   +      +   RH+
Sbjct: 478 LRHAGGHGEWHLFDGSWQLTLQLPEPGRRPEAILQAILRQLALPVASLTPSPESIAIRHL 537

Query: 154 RACM---------*RWWCAVLGGGRRFD 98
            A +          + W A L GG   D
Sbjct: 538 MAQLPERLGTSGHQKGWLAALAGGSAED 565


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,919,092
Number of Sequences: 219361
Number of extensions: 690734
Number of successful extensions: 2570
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 2497
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2564
length of database: 80,573,946
effective HSP length: 91
effective length of database: 60,612,095
effective search space used: 1454690280
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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