ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl07e08
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane pro... 209 3e-54
2AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane pro... 166 2e-41
3AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane pr... 161 8e-40
4HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.... 37 0.030
5HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.... 37 0.030
6HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6... 33 0.33
7HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.... 32 0.56
8HPPA_BRAJA (Q89K83) Pyrophosphate-energized proton pump (EC 3.6.... 30 3.6
9HPPA1_RHOPA (P60363) Pyrophosphate-energized proton pump (EC 3.6... 28 8.1
10HPPA1_METAC (Q8TJA9) Pyrophosphate-energized proton pump 1 (EC 3... 28 8.1

>AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase)
          Length = 762

 Score =  209 bits (532), Expect = 3e-54
 Identities = 105/114 (92%), Positives = 105/114 (92%)
 Frame = +2

Query: 107 MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXKNGYGDYLIEEEE 286
           MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTP         KNGYGDYLIEEEE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAAAGAKNGYGDYLIEEEE 60

Query: 287 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGF 448
           GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGF
Sbjct: 61  GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGF 114



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>AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase) (Vacuolar
           H(+)-pyrophosphatase)
          Length = 764

 Score =  166 bits (420), Expect = 2e-41
 Identities = 83/114 (72%), Positives = 93/114 (81%), Gaps = 1/114 (0%)
 Frame = +2

Query: 110 AILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXX-XXXXKNGYGDYLIEEEE 286
           AIL +LGTEILIPVC VIGI FA+ QW +VSKVK++           KNGY DYLIEEEE
Sbjct: 3   AILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPNAAAKNGYNDYLIEEEE 62

Query: 287 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGF 448
           G+NDHNVVVKCAEIQ AISEGATSFLFT Y+YVG+FMV FA +IFLFLGS+EGF
Sbjct: 63  GINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGF 116



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>AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane proton pump 1|
           (EC 3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase 1) (H(+)-PPase 1) (Vacuolar proton
           pyrophosphatase 1) (Vacuolar proton pyrophosphatase 3)
          Length = 770

 Score =  161 bits (407), Expect = 8e-40
 Identities = 78/121 (64%), Positives = 96/121 (79%), Gaps = 4/121 (3%)
 Frame = +2

Query: 98  LTAMAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXX----KNGYGD 265
           + A A+L EL TEIL+P+C VIGI F++ QW++VS+VK+T              KNGYGD
Sbjct: 1   MVAPALLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGD 60

Query: 266 YLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEG 445
           YLIEEEEG+ND +VV KCAEIQTAISEGATSFLFT Y+YVG+FM+ FAA+IF+FLGS+EG
Sbjct: 61  YLIEEEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEG 120

Query: 446 F 448
           F
Sbjct: 121 F 121



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>HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 36.6 bits (83), Expect = 0.030
 Identities = 20/47 (42%), Positives = 28/47 (59%), Gaps = 2/47 (4%)
 Frame = +2

Query: 314 KCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGS--IEGF 448
           K  EI +AISEGA +FL   Y+ + +F+   A +I L L +   EGF
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGF 88



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>HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 36.6 bits (83), Expect = 0.030
 Identities = 20/47 (42%), Positives = 28/47 (59%), Gaps = 2/47 (4%)
 Frame = +2

Query: 314 KCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGS--IEGF 448
           K  EI +AISEGA +FL   Y+ + +F+   A +I L L +   EGF
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGF 88



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>HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 725

 Score = 33.1 bits (74), Expect = 0.33
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = +2

Query: 323 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSI 439
           EI  AI +GA ++L   Y+ + +   V A +IF FLG +
Sbjct: 40  EIAGAIQQGAAAYLARQYKTIAIVGAVLAILIFFFLGGL 78



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>HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 673

 Score = 32.3 bits (72), Expect = 0.56
 Identities = 15/37 (40%), Positives = 24/37 (64%)
 Frame = +2

Query: 323 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 433
           EI   I +GA +FL T Y+Y+  F+V+   I+ +F+G
Sbjct: 38  EIAGHIHDGAMAFLKTEYKYLTGFIVIVTVILAIFVG 74



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>HPPA_BRAJA (Q89K83) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 706

 Score = 29.6 bits (65), Expect = 3.6
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = +2

Query: 323 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 433
           EI  A+ EGA ++L   Y  +G+  +V   ++  FLG
Sbjct: 37  EIAAAVREGAQAYLRRQYTTIGIVGIVIFVLLVYFLG 73



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>HPPA1_RHOPA (P60363) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 706

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 14/37 (37%), Positives = 21/37 (56%)
 Frame = +2

Query: 323 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 433
           EI  A+ EGA ++L   Y  + +  VV  A++  FLG
Sbjct: 37  EIAGAVREGAQAYLKRQYMTIAIVGVVIFALLAYFLG 73



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>HPPA1_METAC (Q8TJA9) Pyrophosphate-energized proton pump 1 (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase 1)
           (H+-PPase 1) (Membrane-bound proton-translocating
           pyrophosphatase 1)
          Length = 676

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 25/97 (25%), Positives = 39/97 (40%)
 Frame = +2

Query: 140 LIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXKNGYGDYLIEEEEGLNDHNVVVKC 319
           L P+C +IG++FA   +  V                  G G+ LI+              
Sbjct: 7   LAPICALIGLIFAGISYKNVQ---------------NEGAGNDLIK-------------- 37

Query: 320 AEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFL 430
            +I  +I  GA  +L   Y+ + +F+V  A II L L
Sbjct: 38  -KITASIHGGAMVYLNRQYRAIAVFVVFLAIIIALIL 73


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,345,161
Number of Sequences: 219361
Number of extensions: 429038
Number of successful extensions: 1589
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1550
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1583
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2735358828
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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