ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl05g04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane pro... 227 1e-59
2AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane pro... 174 1e-43
3AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane pr... 172 3e-43
4HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.... 37 0.025
5HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.... 37 0.025
6HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6... 33 0.36
7HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.... 32 0.62
8HPPA_BRAJA (Q89K83) Pyrophosphate-energized proton pump (EC 3.6.... 30 4.0
9PRDM1_HUMAN (O75626) PR domain zinc finger protein 1 (PR domain-... 29 5.2
10HPPA_RHORT (O68460) Pyrophosphate-energized proton pump (EC 3.6.... 29 6.8
11HPPA1_METAC (Q8TJA9) Pyrophosphate-energized proton pump 1 (EC 3... 28 8.9
12HPPA1_RHOPA (P60363) Pyrophosphate-energized proton pump (EC 3.6... 28 8.9

>AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase)
          Length = 762

 Score =  227 bits (578), Expect = 1e-59
 Identities = 113/122 (92%), Positives = 113/122 (92%)
 Frame = +3

Query: 99  MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXKNGYGDYLIEEEE 278
           MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTP         KNGYGDYLIEEEE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAAAGAKNGYGDYLIEEEE 60

Query: 279 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 458
           GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP
Sbjct: 61  GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 120

Query: 459 CT 464
           CT
Sbjct: 121 CT 122



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>AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase) (Vacuolar
           H(+)-pyrophosphatase)
          Length = 764

 Score =  174 bits (441), Expect = 1e-43
 Identities = 87/122 (71%), Positives = 98/122 (80%), Gaps = 1/122 (0%)
 Frame = +3

Query: 102 AILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXX-XXXXKNGYGDYLIEEEE 278
           AIL +LGTEILIPVC VIGI FA+ QW +VSKVK++           KNGY DYLIEEEE
Sbjct: 3   AILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPNAAAKNGYNDYLIEEEE 62

Query: 279 GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 458
           G+NDHNVVVKCAEIQ AISEGATSFLFT Y+YVG+FMV FA +IFLFLGS+EGFST  Q 
Sbjct: 63  GINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFSTSPQA 122

Query: 459 CT 464
           C+
Sbjct: 123 CS 124



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>AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane proton pump 1|
           (EC 3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase 1) (H(+)-PPase 1) (Vacuolar proton
           pyrophosphatase 1) (Vacuolar proton pyrophosphatase 3)
          Length = 770

 Score =  172 bits (437), Expect = 3e-43
 Identities = 83/129 (64%), Positives = 102/129 (79%), Gaps = 4/129 (3%)
 Frame = +3

Query: 90  LTAMAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXX----KNGYGD 257
           + A A+L EL TEIL+P+C VIGI F++ QW++VS+VK+T              KNGYGD
Sbjct: 1   MVAPALLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGD 60

Query: 258 YLIEEEEGLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEG 437
           YLIEEEEG+ND +VV KCAEIQTAISEGATSFLFT Y+YVG+FM+ FAA+IF+FLGS+EG
Sbjct: 61  YLIEEEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEG 120

Query: 438 FSTKGQPCT 464
           FST  +PCT
Sbjct: 121 FSTDNKPCT 129



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>HPPA_LEPIN (Q8F641) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 37.0 bits (84), Expect = 0.025
 Identities = 20/48 (41%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
 Frame = +3

Query: 306 KCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGS--IEGFS 443
           K  EI +AISEGA +FL   Y+ + +F+   A +I L L +   EGF+
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN 89



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>HPPA_LEPIC (Q72Q29) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 704

 Score = 37.0 bits (84), Expect = 0.025
 Identities = 20/48 (41%), Positives = 29/48 (60%), Gaps = 2/48 (4%)
 Frame = +3

Query: 306 KCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGS--IEGFS 443
           K  EI +AISEGA +FL   Y+ + +F+   A +I L L +   EGF+
Sbjct: 42  KLLEISSAISEGAMAFLVREYKVISLFIAFMAVLIVLLLDNPGSEGFN 89



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>HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 725

 Score = 33.1 bits (74), Expect = 0.36
 Identities = 15/39 (38%), Positives = 23/39 (58%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSI 431
           EI  AI +GA ++L   Y+ + +   V A +IF FLG +
Sbjct: 40  EIAGAIQQGAAAYLARQYKTIAIVGAVLAILIFFFLGGL 78



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>HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 673

 Score = 32.3 bits (72), Expect = 0.62
 Identities = 15/37 (40%), Positives = 24/37 (64%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 425
           EI   I +GA +FL T Y+Y+  F+V+   I+ +F+G
Sbjct: 38  EIAGHIHDGAMAFLKTEYKYLTGFIVIVTVILAIFVG 74



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>HPPA_BRAJA (Q89K83) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 706

 Score = 29.6 bits (65), Expect = 4.0
 Identities = 13/37 (35%), Positives = 21/37 (56%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 425
           EI  A+ EGA ++L   Y  +G+  +V   ++  FLG
Sbjct: 37  EIAAAVREGAQAYLRRQYTTIGIVGIVIFVLLVYFLG 73



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>PRDM1_HUMAN (O75626) PR domain zinc finger protein 1 (PR domain-containing|
           protein 1) (Beta-interferon gene positive-regulatory
           domain I-binding factor) (BLIMP-1) (Positive regulatory
           domain I-binding factor 1) (PRDI-binding factor 1)
           (PRDI-BF1)
          Length = 789

 Score = 29.3 bits (64), Expect = 5.2
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = -2

Query: 454 WPFVLNPSIDPRKRKIIAAKTTMNIPTYWYMVKRNDVAPSEMAVW 320
           W   +NP+  PR++ + A +  MNI  Y+Y +K    A  E+ VW
Sbjct: 122 WMRYVNPAHSPREQNLAACQNGMNI--YFYTIKPIP-ANQELLVW 163



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>HPPA_RHORT (O68460) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 702

 Score = 28.9 bits (63), Expect = 6.8
 Identities = 15/42 (35%), Positives = 22/42 (52%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGF 440
           EI  A+ EGA++FL   Y+ + +   V   I+   LG   GF
Sbjct: 38  EISGAVQEGASAFLNRQYKTIAVVGAVVFVILTALLGISVGF 79



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>HPPA1_METAC (Q8TJA9) Pyrophosphate-energized proton pump 1 (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase 1)
           (H+-PPase 1) (Membrane-bound proton-translocating
           pyrophosphatase 1)
          Length = 676

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 25/97 (25%), Positives = 39/97 (40%)
 Frame = +3

Query: 132 LIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXKNGYGDYLIEEEEGLNDHNVVVKC 311
           L P+C +IG++FA   +  V                  G G+ LI+              
Sbjct: 7   LAPICALIGLIFAGISYKNVQ---------------NEGAGNDLIK-------------- 37

Query: 312 AEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFL 422
            +I  +I  GA  +L   Y+ + +F+V  A II L L
Sbjct: 38  -KITASIHGGAMVYLNRQYRAIAVFVVFLAIIIALIL 73



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>HPPA1_RHOPA (P60363) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 706

 Score = 28.5 bits (62), Expect = 8.9
 Identities = 14/37 (37%), Positives = 21/37 (56%)
 Frame = +3

Query: 315 EIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLG 425
           EI  A+ EGA ++L   Y  + +  VV  A++  FLG
Sbjct: 37  EIAGAVREGAQAYLKRQYMTIAIVGVVIFALLAYFLG 73


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,874,454
Number of Sequences: 219361
Number of extensions: 504093
Number of successful extensions: 1883
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1833
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1877
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 3026354448
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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