ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl05e12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SIZ1_ARATH (Q680Q4) Sumoylation ligase E3 (EC 6.-.-.-) (SUMO E3 ... 73 3e-13
2PIAS3_RAT (O70260) Protein inhibitor of activated STAT protein 3... 33 0.21
3PIAS3_MOUSE (O54714) Protein inhibitor of activated STAT protein 3 33 0.21
4PIAS3_HUMAN (Q9Y6X2) Protein inhibitor of activated STAT protein 3 33 0.21
5PIAS4_HUMAN (Q8N2W9) Protein inhibitor of activated STAT protein... 33 0.36
6PIAS4_MOUSE (Q9JM05) Protein inhibitor of activated STAT protein... 33 0.36
7MKL1_HUMAN (Q969V6) MKL/myocardin-like protein 1 (Myocardin-rela... 32 0.47
8MKL1_MOUSE (Q8K4J6) MKL/myocardin-like protein 1 (Myocardin-rela... 32 0.61
9REPS_STRAM (P36891) Replication initiator protein 30 1.8
10KU70_CHICK (O93257) ATP-dependent DNA helicase 2 subunit 1 (EC 3... 30 2.3
11KU70_MOUSE (P23475) ATP-dependent DNA helicase 2 subunit 1 (EC 3... 29 5.2
12PLI1_SCHPO (O94451) Sumoylation ligase E3 (EC 6.-.-.-) (Sumoylat... 29 5.2
13HCC1_MOUSE (Q9D1J3) Nuclear protein Hcc-1 28 6.7
14RPOA_OENHO (Q9MTJ3) DNA-directed RNA polymerase alpha chain (EC ... 28 8.8
15CPB1_CAEBR (Q6E3D0) Cytoplasmic polyadenylation element-binding ... 28 8.8
16LOX23_HORVU (Q8GSM2) Lipoxygenase 2.3, chloroplast precursor (EC... 28 8.8

>SIZ1_ARATH (Q680Q4) Sumoylation ligase E3 (EC 6.-.-.-) (SUMO E3 ligase)|
           (AtSIZ1)
          Length = 884

 Score = 72.8 bits (177), Expect = 3e-13
 Identities = 41/63 (65%), Positives = 46/63 (73%)
 Frame = +3

Query: 231 AGCKGKLKHFRIKELKDVLHLLGLSKQGKKQELVDKILAILSDQQDQVPQLNGLTKKPAV 410
           A CK KL +FRIKELKDVL  LGLSKQGKKQELVD+IL +LSD+Q        L+KK  V
Sbjct: 5   ANCKEKLSYFRIKELKDVLTQLGLSKQGKKQELVDRILTLLSDEQ----AARLLSKKNTV 60

Query: 411 EKE 419
            KE
Sbjct: 61  AKE 63



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>PIAS3_RAT (O70260) Protein inhibitor of activated STAT protein 3 (Potassium|
           channel-associated protein) (KChAP)
          Length = 628

 Score = 33.5 bits (75), Expect = 0.21
 Identities = 18/41 (43%), Positives = 26/41 (63%), Gaps = 4/41 (9%)
 Frame = +3

Query: 243 GKLKH----FRIKELKDVLHLLGLSKQGKKQELVDKILAIL 353
           G+LKH    FR+ EL+ +L   G +K G+K EL+ K L +L
Sbjct: 5   GELKHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLL 45



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>PIAS3_MOUSE (O54714) Protein inhibitor of activated STAT protein 3|
          Length = 628

 Score = 33.5 bits (75), Expect = 0.21
 Identities = 18/41 (43%), Positives = 26/41 (63%), Gaps = 4/41 (9%)
 Frame = +3

Query: 243 GKLKH----FRIKELKDVLHLLGLSKQGKKQELVDKILAIL 353
           G+LKH    FR+ EL+ +L   G +K G+K EL+ K L +L
Sbjct: 5   GELKHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLL 45



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>PIAS3_HUMAN (Q9Y6X2) Protein inhibitor of activated STAT protein 3|
          Length = 628

 Score = 33.5 bits (75), Expect = 0.21
 Identities = 18/41 (43%), Positives = 26/41 (63%), Gaps = 4/41 (9%)
 Frame = +3

Query: 243 GKLKH----FRIKELKDVLHLLGLSKQGKKQELVDKILAIL 353
           G+LKH    FR+ EL+ +L   G +K G+K EL+ K L +L
Sbjct: 5   GELKHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLL 45



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>PIAS4_HUMAN (Q8N2W9) Protein inhibitor of activated STAT protein 4 (Protein|
           inhibitor of activated STAT protein gamma) (PIAS-gamma)
           (PIASy)
          Length = 510

 Score = 32.7 bits (73), Expect = 0.36
 Identities = 20/54 (37%), Positives = 30/54 (55%)
 Frame = +3

Query: 222 AVLAGCKGKLKHFRIKELKDVLHLLGLSKQGKKQELVDKILAILSDQQDQVPQL 383
           A L   K  +  FR+ +L+ +L  +G SK G K ELV + L ++  Q D  P+L
Sbjct: 3   AELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLV--QFDCSPEL 54



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>PIAS4_MOUSE (Q9JM05) Protein inhibitor of activated STAT protein 4 (Protein|
           inhibitor of activated STAT protein gamma) (PIAS-gamma)
           (PIASy)
          Length = 507

 Score = 32.7 bits (73), Expect = 0.36
 Identities = 20/54 (37%), Positives = 30/54 (55%)
 Frame = +3

Query: 222 AVLAGCKGKLKHFRIKELKDVLHLLGLSKQGKKQELVDKILAILSDQQDQVPQL 383
           A L   K  +  FR+ +L+ +L  +G SK G K ELV + L ++  Q D  P+L
Sbjct: 3   AELVEAKNMVMSFRVSDLQMLLGFVGRSKSGLKHELVTRALQLV--QFDCSPEL 54



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>MKL1_HUMAN (Q969V6) MKL/myocardin-like protein 1 (Myocardin-related|
           transcription factor A) (MRTF-A) (Megakaryoblastic
           leukemia 1 protein) (Megacaryocytic acute leukemia
           protein)
          Length = 931

 Score = 32.3 bits (72), Expect = 0.47
 Identities = 19/53 (35%), Positives = 27/53 (50%)
 Frame = +3

Query: 249 LKHFRIKELKDVLHLLGLSKQGKKQELVDKILAILSDQQDQVPQLNGLTKKPA 407
           L   ++ ELK  L L  L   G K EL++++ A     QDQ+  + G  K PA
Sbjct: 347 LDDMKVAELKQELKLRSLPVSGTKTELIERLRA----YQDQISPVPGAPKAPA 395



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>MKL1_MOUSE (Q8K4J6) MKL/myocardin-like protein 1 (Myocardin-related|
           transcription factor A) (MRTF-A) (Megakaryoblastic
           leukemia 1 protein homolog) (Basic SAP coiled-coil
           transcription activator)
          Length = 964

 Score = 32.0 bits (71), Expect = 0.61
 Identities = 20/53 (37%), Positives = 26/53 (49%)
 Frame = +3

Query: 249 LKHFRIKELKDVLHLLGLSKQGKKQELVDKILAILSDQQDQVPQLNGLTKKPA 407
           L   ++ ELK  L L  L   G K EL++++ A     QDQV    G  K PA
Sbjct: 385 LDDMKVAELKQELKLRSLPVSGTKTELIERLRA----YQDQVSPAPGAPKAPA 433



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>REPS_STRAM (P36891) Replication initiator protein|
          Length = 459

 Score = 30.4 bits (67), Expect = 1.8
 Identities = 14/33 (42%), Positives = 18/33 (54%), Gaps = 4/33 (12%)
 Frame = +2

Query: 254 ALPNKRAKRCPASAWTFKARK----EAGIGGQD 340
           A  N+RA RCP+ AWT+         AG+ G D
Sbjct: 73  ACGNRRASRCPSCAWTYAGDTYHLIRAGLAGDD 105



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>KU70_CHICK (O93257) ATP-dependent DNA helicase 2 subunit 1 (EC 3.6.1.-)|
           (ATP-dependent DNA helicase II 70 kDa subunit) (Ku
           autoantigen protein p70 homolog) (Ku70) (DNA-repair
           protein XRCC6)
          Length = 632

 Score = 30.0 bits (66), Expect = 2.3
 Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
 Frame = +3

Query: 186 ARAAMKP--ESADDAVLAGCK-GKLKHFRIKELKDVLHLLGLSKQGKKQELVDKILAILS 356
           A+A  +P  E ++D++ +  + G L    +  LKD     GL   GKKQEL+D +    S
Sbjct: 571 AQAEKRPKIEISEDSLRSYVQNGTLGKLTVSALKDTCRHYGLRSGGKKQELIDALTEYFS 630



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>KU70_MOUSE (P23475) ATP-dependent DNA helicase 2 subunit 1 (EC 3.6.1.-)|
           (ATP-dependent DNA helicase II 70 kDa subunit) (Ku
           autoantigen protein p70 homolog) (Ku70) (CTC box-binding
           factor 75 kDa subunit) (CTCBF) (CTC75) (DNA-repair
           protein XRCC6)
          Length = 607

 Score = 28.9 bits (63), Expect = 5.2
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +3

Query: 240 KGKLKHFRIKELKDVLHLLGLSKQGKKQELVDKIL 344
           KG L    +  LKD+    GL    KKQEL+D ++
Sbjct: 567 KGTLGKLTVPTLKDICKAHGLKSGPKKQELLDALI 601



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>PLI1_SCHPO (O94451) Sumoylation ligase E3 (EC 6.-.-.-) (Sumoylation-protein|
           ligase pli1)
          Length = 727

 Score = 28.9 bits (63), Expect = 5.2
 Identities = 16/51 (31%), Positives = 28/51 (54%)
 Frame = +3

Query: 264 IKELKDVLHLLGLSKQGKKQELVDKILAILSDQQDQVPQLNGLTKKPAVEK 416
           I +LKD+L + GL   G K EL+ +I  ++    +++   N  T   A++K
Sbjct: 23  IPQLKDILRVFGLRLSGTKAELITRIKQLI----ERIAIENNTTSWEALKK 69



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>HCC1_MOUSE (Q9D1J3) Nuclear protein Hcc-1|
          Length = 209

 Score = 28.5 bits (62), Expect = 6.7
 Identities = 13/42 (30%), Positives = 24/42 (57%)
 Frame = +3

Query: 246 KLKHFRIKELKDVLHLLGLSKQGKKQELVDKILAILSDQQDQ 371
           +L   ++ ELK      GL  +G KQ+L++++ A L D  ++
Sbjct: 6   ELHKLKLAELKQECLARGLETKGIKQDLINRLQAYLEDHAEE 47



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>RPOA_OENHO (Q9MTJ3) DNA-directed RNA polymerase alpha chain (EC 2.7.7.6) (PEP)|
           (Plastid-encoded RNA polymerase alpha subunit) (RNA
           polymerase alpha subunit)
          Length = 367

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 10/21 (47%), Positives = 17/21 (80%)
 Frame = +3

Query: 246 KLKHFRIKELKDVLHLLGLSK 308
           K+KHFRI+++K +L +L + K
Sbjct: 309 KMKHFRIEDVKQILDILEIEK 329



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>CPB1_CAEBR (Q6E3D0) Cytoplasmic polyadenylation element-binding protein 1|
          Length = 585

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 13/36 (36%), Positives = 21/36 (58%)
 Frame = -2

Query: 298 PSRCRTSFSSFIRKCFNLPLQPASTASSADSGFMAA 191
           PS   T F+  ++   N+P QP S++ +A   F+AA
Sbjct: 126 PSMDLTQFTEELQAIQNMPFQPISSSQAALQAFLAA 161



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>LOX23_HORVU (Q8GSM2) Lipoxygenase 2.3, chloroplast precursor (EC 1.13.11.12)|
           (LOX2:Hv:3)
          Length = 896

 Score = 28.1 bits (61), Expect = 8.8
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = +2

Query: 161 ASSHWIAPGQSRHEAGVCRRCSARRLQGQ 247
           AS  ++A GQ R  +G  R CS RRL  +
Sbjct: 18  ASPLFVAGGQQRRASGAGRTCSGRRLSAR 46


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,934,298
Number of Sequences: 219361
Number of extensions: 586512
Number of successful extensions: 2313
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 2278
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2313
length of database: 80,573,946
effective HSP length: 100
effective length of database: 58,637,846
effective search space used: 2286875994
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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