Clone Name | bastl03d12 |
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Clone Library Name | barley_pub |
>DNAK_RHILO (Q98DD1) Chaperone protein dnaK (Heat shock protein 70) (Heat shock| 70 kDa protein) (HSP70) Length = 638 Score = 32.3 bits (72), Expect = 0.67 Identities = 31/106 (29%), Positives = 47/106 (44%), Gaps = 13/106 (12%) Frame = +2 Query: 14 SECVVLSSVRVQAENG--VCEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEAS 187 +E +V SS + E G V EA + D IA K ++ D D + +++ L EAS Sbjct: 534 AEALVHSSEKSLKEYGDKVSEAERTAISDAIAALKTAAEGDDAADI---EAKSQALAEAS 590 Query: 188 HGLEQV-----------ATGQLDLGAVNIMEDADSEEVAYDEENKK 292 L Q A + D + + DAD EE+ D++ KK Sbjct: 591 MKLGQAMYEASQKEAAEADAKADAAKDSDVVDADFEEIDEDDDKKK 636
>MDN1_YEAST (Q12019) Midasin (MIDAS-containing protein)| Length = 4910 Score = 31.6 bits (70), Expect = 1.2 Identities = 24/74 (32%), Positives = 35/74 (47%) Frame = +2 Query: 113 AVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKK 292 A +++ + QD E+D ENE G L G N ED DSEE DEE Sbjct: 4087 AQNENKEQQDKDERDDENEDDAVEMEGDMAGELEDLSNGEENDDEDTDSEEEELDEE-ID 4145 Query: 293 PVHPTDMNSLCEKI 334 ++ D N++ +K+ Sbjct: 4146 DLNEDDPNAIDDKM 4159
>PTB_CLOAB (P58255) Phosphate butyryltransferase (EC 2.3.1.19)| (Phosphotransbutyrylase) Length = 301 Score = 31.2 bits (69), Expect = 1.5 Identities = 25/76 (32%), Positives = 37/76 (48%), Gaps = 5/76 (6%) Frame = +2 Query: 26 VLSSVRVQAENGVCEANLGSDHDTIA--YFKAVSKSGDFQDTSEQDGENEGLTEASHGLE 199 VL +VR +NG+ +A L DHD I K DF+ +E + + L +E Sbjct: 30 VLEAVRDAKKNGIADAILVGDHDEIVSIALKIGMDVNDFEIVNEPNVKKAAL----KAVE 85 Query: 200 QVATGQLDL---GAVN 238 V+TG+ D+ G VN Sbjct: 86 LVSTGKADMVMKGLVN 101
>PPCK_HAEIN (P43923) Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP| carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK) Length = 538 Score = 30.4 bits (67), Expect = 2.6 Identities = 20/79 (25%), Positives = 34/79 (43%) Frame = +2 Query: 65 CEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLGAVNIM 244 C AN+G D D +F SG + T D + E + + HG + D+G N Sbjct: 231 CSANVGKDGDVAIFF---GLSGTGKTTLSTDPKRELIGDDEHGWD-------DVGIFNFE 280 Query: 245 EDADSEEVAYDEENKKPVH 301 ++ + EEN+ ++ Sbjct: 281 GGCYAKTIHLSEENEPDIY 299
>YJX4_SCHPO (Q9UU99) Protein C23B6.04c in chromosome III| Length = 1008 Score = 30.0 bits (66), Expect = 3.3 Identities = 26/105 (24%), Positives = 48/105 (45%), Gaps = 3/105 (2%) Frame = +2 Query: 5 EEPSECVVLSSVRVQAENGVCEANLGSDHDTIAYFKAVSKSGDFQ---DTSEQDGENEGL 175 ++P+E V ++V ++E VC+ N ++K S GD + E+DG + L Sbjct: 231 KQPTEHPVSANVPAKSEKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLERDGRDTSL 290 Query: 176 TEASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKKPVHPTD 310 +AS A + D+ + + S+E A E+ K H ++ Sbjct: 291 GDAS---VNEAAKETDVDSSRFI----SDEKAVTEDAMKTEHASN 328
>NNP1_MOUSE (P56183) NNP-1 protein (Novel nuclear protein 1) (Nop52)| Length = 494 Score = 29.6 bits (65), Expect = 4.4 Identities = 25/96 (26%), Positives = 39/96 (40%), Gaps = 7/96 (7%) Frame = +2 Query: 68 EANLGSDHDTIAYFKAVSKSGDFQDTSEQDG------ENEGLTEASHGLEQVATGQLDLG 229 EA+ G D D + D D S+ DG ++ +E L+ +L G Sbjct: 268 EASDGGDGDVADSDDSDGADDDDGDVSDGDGGDNDEGDSNKSSEGEQDLQDTPPKKLPAG 327 Query: 230 AVNIM-EDADSEEVAYDEENKKPVHPTDMNSLCEKI 334 + +AD E+ DEEN PV D +L ++ Sbjct: 328 TAHRAGPEADKEQAWDDEENAGPVLQFDYEALANRL 363
>PPCK_LEPIN (Q8F9E4) Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP| carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK) Length = 530 Score = 29.6 bits (65), Expect = 4.4 Identities = 29/114 (25%), Positives = 44/114 (38%), Gaps = 16/114 (14%) Frame = +2 Query: 65 CEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLG----A 232 C AN+G D DT +F SG + T D + + + HG + ++ G Sbjct: 219 CSANVGQDGDTALFF---GLSGTGKTTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKV 275 Query: 233 VN------------IMEDADSEEVAYDEENKKPVHPTDMNSLCEKINVSRPVEH 358 +N I +DA E V YD + K + + E VS P+ H Sbjct: 276 INLDPKTEPDIYEAIRKDALLENVVYDPQTK--IVDYSSAAKTENTRVSYPIFH 327
>PPCK_LEPIC (Q72VT0) Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP| carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK) Length = 530 Score = 29.6 bits (65), Expect = 4.4 Identities = 29/114 (25%), Positives = 44/114 (38%), Gaps = 16/114 (14%) Frame = +2 Query: 65 CEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLG----A 232 C AN+G D DT +F SG + T D + + + HG + ++ G Sbjct: 219 CSANVGQDGDTALFF---GLSGTGKTTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKV 275 Query: 233 VN------------IMEDADSEEVAYDEENKKPVHPTDMNSLCEKINVSRPVEH 358 +N I +DA E V YD + K + + E VS P+ H Sbjct: 276 INLDPKTEPDIYEAIRKDALLENVVYDPQTK--IVDYSSAAKTENTRVSYPIFH 327
>PP2CG_BOVIN (P79126) Protein phosphatase 2C isoform gamma (EC 3.1.3.16)| (PP2C-gamma) (Protein phosphatase magnesium-dependent 1 gamma) (Protein phosphatase 1B) (Magnesium-dependent calcium inhibitable phosphatase) (MCPP) Length = 543 Score = 29.6 bits (65), Expect = 4.4 Identities = 18/63 (28%), Positives = 30/63 (47%) Frame = +2 Query: 116 VSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKKP 295 V KS F+D+ ++ E E E S + G A N ++ D+EE D+E ++ Sbjct: 251 VDKSKFFEDSEDESDEAEEEEEDSEECSEEEDGYSSEEAENEEDEDDTEEAEEDDEEEEM 310 Query: 296 VHP 304 + P Sbjct: 311 MVP 313
>APS_RAT (Q9Z200) SH2 and PH domain-containing adapter protein APS (Adapter| protein with pleckstrin homology and Src homology 2 domains) Length = 621 Score = 29.6 bits (65), Expect = 4.4 Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 1/34 (2%) Frame = -3 Query: 447 RQGLELRSTSLLSVEGISDLLNKE-SPRPEACST 349 R+G LR+ SL V+G+ DL ++ SP PE +T Sbjct: 121 RKGFSLRNMSLCVVDGVRDLWHRRASPEPEGGAT 154
>143G1_ONCMY (Q6UFZ3) 14-3-3 protein gamma-1 (Protein 14-3-3G1)| Length = 247 Score = 29.3 bits (64), Expect = 5.7 Identities = 14/37 (37%), Positives = 21/37 (56%) Frame = +2 Query: 194 LEQVATGQLDLGAVNIMEDADSEEVAYDEENKKPVHP 304 L +VATG+ +G V E + SE +E+ +P HP Sbjct: 134 LAEVATGEKRVGVVESSEKSYSEAHEISKEHMQPTHP 170
>PP2CG_HUMAN (O15355) Protein phosphatase 2C isoform gamma (EC 3.1.3.16)| (PP2C-gamma) (Protein phosphatase magnesium-dependent 1 gamma) (Protein phosphatase 1C) Length = 546 Score = 28.9 bits (63), Expect = 7.5 Identities = 17/59 (28%), Positives = 29/59 (49%) Frame = +2 Query: 116 VSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKK 292 V+KS F+D+ ++ E E E S + G A N ++ D+EE D+E ++ Sbjct: 251 VAKSKFFEDSEDESDEAEEEEEDSEECSEEEDGYSSEEAENEEDEDDTEEAEEDDEEEE 309
>CHE1_CAEEL (Q966L8) Transcription factor che-1 (Abnormal chemotaxis protein 1)| Length = 279 Score = 28.9 bits (63), Expect = 7.5 Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 7/56 (12%) Frame = +2 Query: 47 QAENGVCEANLGSDHDTIAYFKAVS--KSGDFQDTSEQDGENE-----GLTEASHG 193 Q ENG+ + GSDH +F+A + D+ TS N+ G SHG Sbjct: 54 QTENGIIQGQNGSDHGVAFHFRAYGGWRQHDWNSTSSWSIGNQLEMENGTLTMSHG 109
>INO80_KLULA (Q6CNY4) Putative DNA helicase INO80 (EC 3.6.1.-)| Length = 1489 Score = 28.9 bits (63), Expect = 7.5 Identities = 25/103 (24%), Positives = 47/103 (45%), Gaps = 4/103 (3%) Frame = +2 Query: 56 NGVCEANLGSDHDTI--AYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLG 229 NGV E N DHD++ A A+ S D + +++ ++E + E + A + D+ Sbjct: 300 NGVTEGNEDMDHDSVLDADDSAIG-SDDNPNDEQEEADDENVDEEAEDENDEAEVEDDID 358 Query: 230 AVNIMEDADSEEVA--YDEENKKPVHPTDMNSLCEKINVSRPV 352 + ED ++ A Y +K V+ +K+N+ P+ Sbjct: 359 DLGAPEDDGDDDFAPSYSAISKVKVN--------KKVNIDSPM 393
>PLK2_HUMAN (Q9NYY3) Serine/threonine-protein kinase PLK2 (EC 2.7.11.21)| (Polo-like kinase 1) (PLK-2) (Serine/threonine-protein kinase SNK) (Serum-inducible kinase) Length = 685 Score = 28.9 bits (63), Expect = 7.5 Identities = 15/39 (38%), Positives = 18/39 (46%) Frame = -1 Query: 251 RPPLYLQPPNLADQLPPALVHD*PPLILHFHHLAQKYPE 135 +PP QPP Q+PPA H H HH + PE Sbjct: 37 QPPEESQPPQSQAQVPPAAPH-------HHHHHSHSGPE 68
>PPCK_THETN (Q8R943) Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP| carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK) Length = 521 Score = 28.5 bits (62), Expect = 9.7 Identities = 25/113 (22%), Positives = 44/113 (38%), Gaps = 16/113 (14%) Frame = +2 Query: 65 CEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLG----A 232 C AN+G D DT +F SG + T D E + + HG + + G Sbjct: 212 CSANIGKDGDTALFF---GLSGTGKTTLSADPERFLIGDDEHGWSERGIFNFEGGCYAKC 268 Query: 233 VNIMEDADS------------EEVAYDEENKKPVHPTDMNSLCEKINVSRPVE 355 +N+ + + E V YDE ++ + +D + E + P++ Sbjct: 269 INLSREKEPQIWDSIRFGAILENVVYDEATRELDYTSD--KITENTRAAYPID 319
>APS_HUMAN (O14492) SH2 and PH domain-containing adapter protein APS (Adapter| protein with pleckstrin homology and Src homology 2 domains) Length = 632 Score = 28.5 bits (62), Expect = 9.7 Identities = 13/33 (39%), Positives = 22/33 (66%), Gaps = 1/33 (3%) Frame = -3 Query: 447 RQGLELRSTSLLSVEGISDLLNKE-SPRPEACS 352 R+G LR+ SL V+G+ D+ ++ SP P+A + Sbjct: 132 RKGFSLRNMSLCVVDGVRDMWHRRASPEPDAAA 164
>METX_PELUB (Q4FP51) Homoserine O-acetyltransferase (EC 2.3.1.31) (Homoserine| O-trans-acetylase) (Homoserine transacetylase) (HTA) Length = 372 Score = 28.5 bits (62), Expect = 9.7 Identities = 15/38 (39%), Positives = 21/38 (55%) Frame = +2 Query: 182 ASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKKP 295 ASH + +A +L G +IM DA+ E Y +NK P Sbjct: 175 ASHSAQNIAFNEL--GRQSIMADANWENGDYSNQNKNP 210 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 66,072,365 Number of Sequences: 219361 Number of extensions: 1327501 Number of successful extensions: 3926 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 3520 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 3878 length of database: 80,573,946 effective HSP length: 103 effective length of database: 57,979,763 effective search space used: 3304846491 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)