ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl03d12
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1DNAK_RHILO (Q98DD1) Chaperone protein dnaK (Heat shock protein 7... 32 0.67
2MDN1_YEAST (Q12019) Midasin (MIDAS-containing protein) 32 1.2
3PTB_CLOAB (P58255) Phosphate butyryltransferase (EC 2.3.1.19) (P... 31 1.5
4PPCK_HAEIN (P43923) Phosphoenolpyruvate carboxykinase [ATP] (EC ... 30 2.6
5YJX4_SCHPO (Q9UU99) Protein C23B6.04c in chromosome III 30 3.3
6NNP1_MOUSE (P56183) NNP-1 protein (Novel nuclear protein 1) (Nop52) 30 4.4
7PPCK_LEPIN (Q8F9E4) Phosphoenolpyruvate carboxykinase [ATP] (EC ... 30 4.4
8PPCK_LEPIC (Q72VT0) Phosphoenolpyruvate carboxykinase [ATP] (EC ... 30 4.4
9PP2CG_BOVIN (P79126) Protein phosphatase 2C isoform gamma (EC 3.... 30 4.4
10APS_RAT (Q9Z200) SH2 and PH domain-containing adapter protein AP... 30 4.4
11143G1_ONCMY (Q6UFZ3) 14-3-3 protein gamma-1 (Protein 14-3-3G1) 29 5.7
12PP2CG_HUMAN (O15355) Protein phosphatase 2C isoform gamma (EC 3.... 29 7.5
13CHE1_CAEEL (Q966L8) Transcription factor che-1 (Abnormal chemota... 29 7.5
14INO80_KLULA (Q6CNY4) Putative DNA helicase INO80 (EC 3.6.1.-) 29 7.5
15PLK2_HUMAN (Q9NYY3) Serine/threonine-protein kinase PLK2 (EC 2.7... 29 7.5
16PPCK_THETN (Q8R943) Phosphoenolpyruvate carboxykinase [ATP] (EC ... 28 9.7
17APS_HUMAN (O14492) SH2 and PH domain-containing adapter protein ... 28 9.7
18METX_PELUB (Q4FP51) Homoserine O-acetyltransferase (EC 2.3.1.31)... 28 9.7

>DNAK_RHILO (Q98DD1) Chaperone protein dnaK (Heat shock protein 70) (Heat shock|
           70 kDa protein) (HSP70)
          Length = 638

 Score = 32.3 bits (72), Expect = 0.67
 Identities = 31/106 (29%), Positives = 47/106 (44%), Gaps = 13/106 (12%)
 Frame = +2

Query: 14  SECVVLSSVRVQAENG--VCEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEAS 187
           +E +V SS +   E G  V EA   +  D IA  K  ++  D  D    + +++ L EAS
Sbjct: 534 AEALVHSSEKSLKEYGDKVSEAERTAISDAIAALKTAAEGDDAADI---EAKSQALAEAS 590

Query: 188 HGLEQV-----------ATGQLDLGAVNIMEDADSEEVAYDEENKK 292
             L Q            A  + D    + + DAD EE+  D++ KK
Sbjct: 591 MKLGQAMYEASQKEAAEADAKADAAKDSDVVDADFEEIDEDDDKKK 636



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>MDN1_YEAST (Q12019) Midasin (MIDAS-containing protein)|
          Length = 4910

 Score = 31.6 bits (70), Expect = 1.2
 Identities = 24/74 (32%), Positives = 35/74 (47%)
 Frame = +2

Query: 113  AVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKK 292
            A +++ + QD  E+D ENE       G        L  G  N  ED DSEE   DEE   
Sbjct: 4087 AQNENKEQQDKDERDDENEDDAVEMEGDMAGELEDLSNGEENDDEDTDSEEEELDEE-ID 4145

Query: 293  PVHPTDMNSLCEKI 334
             ++  D N++ +K+
Sbjct: 4146 DLNEDDPNAIDDKM 4159



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>PTB_CLOAB (P58255) Phosphate butyryltransferase (EC 2.3.1.19)|
           (Phosphotransbutyrylase)
          Length = 301

 Score = 31.2 bits (69), Expect = 1.5
 Identities = 25/76 (32%), Positives = 37/76 (48%), Gaps = 5/76 (6%)
 Frame = +2

Query: 26  VLSSVRVQAENGVCEANLGSDHDTIA--YFKAVSKSGDFQDTSEQDGENEGLTEASHGLE 199
           VL +VR   +NG+ +A L  DHD I     K      DF+  +E + +   L      +E
Sbjct: 30  VLEAVRDAKKNGIADAILVGDHDEIVSIALKIGMDVNDFEIVNEPNVKKAAL----KAVE 85

Query: 200 QVATGQLDL---GAVN 238
            V+TG+ D+   G VN
Sbjct: 86  LVSTGKADMVMKGLVN 101



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>PPCK_HAEIN (P43923) Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP|
           carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK)
          Length = 538

 Score = 30.4 bits (67), Expect = 2.6
 Identities = 20/79 (25%), Positives = 34/79 (43%)
 Frame = +2

Query: 65  CEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLGAVNIM 244
           C AN+G D D   +F     SG  + T   D + E + +  HG +       D+G  N  
Sbjct: 231 CSANVGKDGDVAIFF---GLSGTGKTTLSTDPKRELIGDDEHGWD-------DVGIFNFE 280

Query: 245 EDADSEEVAYDEENKKPVH 301
               ++ +   EEN+  ++
Sbjct: 281 GGCYAKTIHLSEENEPDIY 299



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>YJX4_SCHPO (Q9UU99) Protein C23B6.04c in chromosome III|
          Length = 1008

 Score = 30.0 bits (66), Expect = 3.3
 Identities = 26/105 (24%), Positives = 48/105 (45%), Gaps = 3/105 (2%)
 Frame = +2

Query: 5   EEPSECVVLSSVRVQAENGVCEANLGSDHDTIAYFKAVSKSGDFQ---DTSEQDGENEGL 175
           ++P+E  V ++V  ++E  VC+ N         ++K  S  GD +      E+DG +  L
Sbjct: 231 KQPTEHPVSANVPAKSEKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLERDGRDTSL 290

Query: 176 TEASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKKPVHPTD 310
            +AS      A  + D+ +   +    S+E A  E+  K  H ++
Sbjct: 291 GDAS---VNEAAKETDVDSSRFI----SDEKAVTEDAMKTEHASN 328



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>NNP1_MOUSE (P56183) NNP-1 protein (Novel nuclear protein 1) (Nop52)|
          Length = 494

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 25/96 (26%), Positives = 39/96 (40%), Gaps = 7/96 (7%)
 Frame = +2

Query: 68  EANLGSDHDTIAYFKAVSKSGDFQDTSEQDG------ENEGLTEASHGLEQVATGQLDLG 229
           EA+ G D D      +     D  D S+ DG      ++   +E    L+     +L  G
Sbjct: 268 EASDGGDGDVADSDDSDGADDDDGDVSDGDGGDNDEGDSNKSSEGEQDLQDTPPKKLPAG 327

Query: 230 AVNIM-EDADSEEVAYDEENKKPVHPTDMNSLCEKI 334
             +    +AD E+   DEEN  PV   D  +L  ++
Sbjct: 328 TAHRAGPEADKEQAWDDEENAGPVLQFDYEALANRL 363



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>PPCK_LEPIN (Q8F9E4) Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP|
           carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK)
          Length = 530

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 29/114 (25%), Positives = 44/114 (38%), Gaps = 16/114 (14%)
 Frame = +2

Query: 65  CEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLG----A 232
           C AN+G D DT  +F     SG  + T   D   + + +  HG +      ++ G     
Sbjct: 219 CSANVGQDGDTALFF---GLSGTGKTTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKV 275

Query: 233 VN------------IMEDADSEEVAYDEENKKPVHPTDMNSLCEKINVSRPVEH 358
           +N            I +DA  E V YD + K  +      +  E   VS P+ H
Sbjct: 276 INLDPKTEPDIYEAIRKDALLENVVYDPQTK--IVDYSSAAKTENTRVSYPIFH 327



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>PPCK_LEPIC (Q72VT0) Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP|
           carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK)
          Length = 530

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 29/114 (25%), Positives = 44/114 (38%), Gaps = 16/114 (14%)
 Frame = +2

Query: 65  CEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLG----A 232
           C AN+G D DT  +F     SG  + T   D   + + +  HG +      ++ G     
Sbjct: 219 CSANVGQDGDTALFF---GLSGTGKTTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKV 275

Query: 233 VN------------IMEDADSEEVAYDEENKKPVHPTDMNSLCEKINVSRPVEH 358
           +N            I +DA  E V YD + K  +      +  E   VS P+ H
Sbjct: 276 INLDPKTEPDIYEAIRKDALLENVVYDPQTK--IVDYSSAAKTENTRVSYPIFH 327



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>PP2CG_BOVIN (P79126) Protein phosphatase 2C isoform gamma (EC 3.1.3.16)|
           (PP2C-gamma) (Protein phosphatase magnesium-dependent 1
           gamma) (Protein phosphatase 1B) (Magnesium-dependent
           calcium inhibitable phosphatase) (MCPP)
          Length = 543

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 18/63 (28%), Positives = 30/63 (47%)
 Frame = +2

Query: 116 VSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKKP 295
           V KS  F+D+ ++  E E   E S    +   G     A N  ++ D+EE   D+E ++ 
Sbjct: 251 VDKSKFFEDSEDESDEAEEEEEDSEECSEEEDGYSSEEAENEEDEDDTEEAEEDDEEEEM 310

Query: 296 VHP 304
           + P
Sbjct: 311 MVP 313



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>APS_RAT (Q9Z200) SH2 and PH domain-containing adapter protein APS (Adapter|
           protein with pleckstrin homology and Src homology 2
           domains)
          Length = 621

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
 Frame = -3

Query: 447 RQGLELRSTSLLSVEGISDLLNKE-SPRPEACST 349
           R+G  LR+ SL  V+G+ DL ++  SP PE  +T
Sbjct: 121 RKGFSLRNMSLCVVDGVRDLWHRRASPEPEGGAT 154



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>143G1_ONCMY (Q6UFZ3) 14-3-3 protein gamma-1 (Protein 14-3-3G1)|
          Length = 247

 Score = 29.3 bits (64), Expect = 5.7
 Identities = 14/37 (37%), Positives = 21/37 (56%)
 Frame = +2

Query: 194 LEQVATGQLDLGAVNIMEDADSEEVAYDEENKKPVHP 304
           L +VATG+  +G V   E + SE     +E+ +P HP
Sbjct: 134 LAEVATGEKRVGVVESSEKSYSEAHEISKEHMQPTHP 170



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>PP2CG_HUMAN (O15355) Protein phosphatase 2C isoform gamma (EC 3.1.3.16)|
           (PP2C-gamma) (Protein phosphatase magnesium-dependent 1
           gamma) (Protein phosphatase 1C)
          Length = 546

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 17/59 (28%), Positives = 29/59 (49%)
 Frame = +2

Query: 116 VSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKK 292
           V+KS  F+D+ ++  E E   E S    +   G     A N  ++ D+EE   D+E ++
Sbjct: 251 VAKSKFFEDSEDESDEAEEEEEDSEECSEEEDGYSSEEAENEEDEDDTEEAEEDDEEEE 309



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>CHE1_CAEEL (Q966L8) Transcription factor che-1 (Abnormal chemotaxis protein 1)|
          Length = 279

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 7/56 (12%)
 Frame = +2

Query: 47  QAENGVCEANLGSDHDTIAYFKAVS--KSGDFQDTSEQDGENE-----GLTEASHG 193
           Q ENG+ +   GSDH    +F+A    +  D+  TS     N+     G    SHG
Sbjct: 54  QTENGIIQGQNGSDHGVAFHFRAYGGWRQHDWNSTSSWSIGNQLEMENGTLTMSHG 109



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>INO80_KLULA (Q6CNY4) Putative DNA helicase INO80 (EC 3.6.1.-)|
          Length = 1489

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 25/103 (24%), Positives = 47/103 (45%), Gaps = 4/103 (3%)
 Frame = +2

Query: 56  NGVCEANLGSDHDTI--AYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLG 229
           NGV E N   DHD++  A   A+  S D  +  +++ ++E + E +      A  + D+ 
Sbjct: 300 NGVTEGNEDMDHDSVLDADDSAIG-SDDNPNDEQEEADDENVDEEAEDENDEAEVEDDID 358

Query: 230 AVNIMEDADSEEVA--YDEENKKPVHPTDMNSLCEKINVSRPV 352
            +   ED   ++ A  Y   +K  V+        +K+N+  P+
Sbjct: 359 DLGAPEDDGDDDFAPSYSAISKVKVN--------KKVNIDSPM 393



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>PLK2_HUMAN (Q9NYY3) Serine/threonine-protein kinase PLK2 (EC 2.7.11.21)|
           (Polo-like kinase 1) (PLK-2) (Serine/threonine-protein
           kinase SNK) (Serum-inducible kinase)
          Length = 685

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 15/39 (38%), Positives = 18/39 (46%)
 Frame = -1

Query: 251 RPPLYLQPPNLADQLPPALVHD*PPLILHFHHLAQKYPE 135
           +PP   QPP    Q+PPA  H       H HH +   PE
Sbjct: 37  QPPEESQPPQSQAQVPPAAPH-------HHHHHSHSGPE 68



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>PPCK_THETN (Q8R943) Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (PEP|
           carboxykinase) (Phosphoenolpyruvate carboxylase) (PEPCK)
          Length = 521

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 25/113 (22%), Positives = 44/113 (38%), Gaps = 16/113 (14%)
 Frame = +2

Query: 65  CEANLGSDHDTIAYFKAVSKSGDFQDTSEQDGENEGLTEASHGLEQVATGQLDLG----A 232
           C AN+G D DT  +F     SG  + T   D E   + +  HG  +      + G     
Sbjct: 212 CSANIGKDGDTALFF---GLSGTGKTTLSADPERFLIGDDEHGWSERGIFNFEGGCYAKC 268

Query: 233 VNIMEDADS------------EEVAYDEENKKPVHPTDMNSLCEKINVSRPVE 355
           +N+  + +             E V YDE  ++  + +D   + E    + P++
Sbjct: 269 INLSREKEPQIWDSIRFGAILENVVYDEATRELDYTSD--KITENTRAAYPID 319



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>APS_HUMAN (O14492) SH2 and PH domain-containing adapter protein APS (Adapter|
           protein with pleckstrin homology and Src homology 2
           domains)
          Length = 632

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 13/33 (39%), Positives = 22/33 (66%), Gaps = 1/33 (3%)
 Frame = -3

Query: 447 RQGLELRSTSLLSVEGISDLLNKE-SPRPEACS 352
           R+G  LR+ SL  V+G+ D+ ++  SP P+A +
Sbjct: 132 RKGFSLRNMSLCVVDGVRDMWHRRASPEPDAAA 164



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>METX_PELUB (Q4FP51) Homoserine O-acetyltransferase (EC 2.3.1.31) (Homoserine|
           O-trans-acetylase) (Homoserine transacetylase) (HTA)
          Length = 372

 Score = 28.5 bits (62), Expect = 9.7
 Identities = 15/38 (39%), Positives = 21/38 (55%)
 Frame = +2

Query: 182 ASHGLEQVATGQLDLGAVNIMEDADSEEVAYDEENKKP 295
           ASH  + +A  +L  G  +IM DA+ E   Y  +NK P
Sbjct: 175 ASHSAQNIAFNEL--GRQSIMADANWENGDYSNQNKNP 210


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,072,365
Number of Sequences: 219361
Number of extensions: 1327501
Number of successful extensions: 3926
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 3520
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3878
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3304846491
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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