ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl03b03
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane pro... 234 7e-62
2AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane pro... 176 3e-44
3AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane pr... 172 3e-43
4HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6... 32 1.1
5HPPA1_METAC (Q8TJA9) Pyrophosphate-energized proton pump 1 (EC 3... 32 1.1
6HPPA1_METMA (Q8PYZ8) Pyrophosphate-energized proton pump 1 (EC 3... 31 1.5
7HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.... 31 1.5
8PRDM1_HUMAN (O75626) PR domain zinc finger protein 1 (PR domain-... 29 7.3

>AVP_HORVU (Q06572) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase)
          Length = 762

 Score =  234 bits (598), Expect = 7e-62
 Identities = 117/127 (92%), Positives = 117/127 (92%)
 Frame = +3

Query: 99  MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXKNGYGDYLIEEEE 278
           MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTP         KNGYGDYLIEEEE
Sbjct: 1   MAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPGAASAAAGAKNGYGDYLIEEEE 60

Query: 279 GLNDHNVVVKCAEIQTAISGGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 458
           GLNDHNVVVKCAEIQTAIS GATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP
Sbjct: 61  GLNDHNVVVKCAEIQTAISEGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 120

Query: 459 CTYSKGT 479
           CTYSKGT
Sbjct: 121 CTYSKGT 127



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>AVP_PHAAU (P21616) Pyrophosphate-energized vacuolar membrane proton pump (EC|
           3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase) (H(+)-PPase) (Vacuolar
           H(+)-pyrophosphatase)
          Length = 764

 Score =  176 bits (446), Expect = 3e-44
 Identities = 88/125 (70%), Positives = 99/125 (79%), Gaps = 1/125 (0%)
 Frame = +3

Query: 102 AILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXX-XXXXKNGYGDYLIEEEE 278
           AIL +LGTEILIPVC VIGI FA+ QW +VSKVK++           KNGY DYLIEEEE
Sbjct: 3   AILPDLGTEILIPVCAVIGIAFALFQWLLVSKVKLSAVRDASPNAAAKNGYNDYLIEEEE 62

Query: 279 GLNDHNVVVKCAEIQTAISGGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEGFSTKGQP 458
           G+NDHNVVVKCAEIQ AIS GATSFLFT Y+YVG+FMV FA +IFLFLGS+EGFST  Q 
Sbjct: 63  GINDHNVVVKCAEIQNAISEGATSFLFTEYKYVGIFMVAFAILIFLFLGSVEGFSTSPQA 122

Query: 459 CTYSK 473
           C+Y K
Sbjct: 123 CSYDK 127



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>AVP1_ARATH (P31414) Pyrophosphate-energized vacuolar membrane proton pump 1|
           (EC 3.6.1.1) (Pyrophosphate-energized inorganic
           pyrophosphatase 1) (H(+)-PPase 1) (Vacuolar proton
           pyrophosphatase 1) (Vacuolar proton pyrophosphatase 3)
          Length = 770

 Score =  172 bits (437), Expect = 3e-43
 Identities = 83/130 (63%), Positives = 102/130 (78%), Gaps = 4/130 (3%)
 Frame = +3

Query: 90  LTAMAILGELGTEILIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXX----KNGYGD 257
           + A A+L EL TEIL+P+C VIGI F++ QW++VS+VK+T              KNGYGD
Sbjct: 1   MVAPALLPELWTEILVPICAVIGIAFSLFQWYVVSRVKLTSDLGASSSGGANNGKNGYGD 60

Query: 258 YLIEEEEGLNDHNVVVKCAEIQTAISGGATSFLFTMYQYVGMFMVVFAAIIFLFLGSIEG 437
           YLIEEEEG+ND +VV KCAEIQTAIS GATSFLFT Y+YVG+FM+ FAA+IF+FLGS+EG
Sbjct: 61  YLIEEEEGVNDQSVVAKCAEIQTAISEGATSFLFTEYKYVGVFMIFFAAVIFVFLGSVEG 120

Query: 438 FSTKGQPCTY 467
           FST  +PCTY
Sbjct: 121 FSTDNKPCTY 130



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>HPPA2_RHOPA (Q8KY01) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 725

 Score = 31.6 bits (70), Expect = 1.1
 Identities = 15/39 (38%), Positives = 22/39 (56%)
 Frame = +3

Query: 315 EIQTAISGGATSFLFTMYQYVGMFMVVFAAIIFLFLGSI 431
           EI  AI  GA ++L   Y+ + +   V A +IF FLG +
Sbjct: 40  EIAGAIQQGAAAYLARQYKTIAIVGAVLAILIFFFLGGL 78



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>HPPA1_METAC (Q8TJA9) Pyrophosphate-energized proton pump 1 (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase 1)
           (H+-PPase 1) (Membrane-bound proton-translocating
           pyrophosphatase 1)
          Length = 676

 Score = 31.6 bits (70), Expect = 1.1
 Identities = 26/97 (26%), Positives = 40/97 (41%)
 Frame = +3

Query: 132 LIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXKNGYGDYLIEEEEGLNDHNVVVKC 311
           L P+C +IG++FA   +  V                  G G+ LI+              
Sbjct: 7   LAPICALIGLIFAGISYKNVQ---------------NEGAGNDLIK-------------- 37

Query: 312 AEIQTAISGGATSFLFTMYQYVGMFMVVFAAIIFLFL 422
            +I  +I GGA  +L   Y+ + +F+V  A II L L
Sbjct: 38  -KITASIHGGAMVYLNRQYRAIAVFVVFLAIIIALIL 73



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>HPPA1_METMA (Q8PYZ8) Pyrophosphate-energized proton pump 1 (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase 1)
           (H+-PPase 1) (Membrane-bound proton-translocating
           pyrophosphatase 1)
          Length = 676

 Score = 31.2 bits (69), Expect = 1.5
 Identities = 25/97 (25%), Positives = 40/97 (41%)
 Frame = +3

Query: 132 LIPVCGVIGIVFAVAQWFIVSKVKVTPXXXXXXXXXKNGYGDYLIEEEEGLNDHNVVVKC 311
           L P+C +IG++FA   +  V                  G G+ LI+              
Sbjct: 7   LTPICALIGLIFAGISYKNV---------------RNEGEGNELIK-------------- 37

Query: 312 AEIQTAISGGATSFLFTMYQYVGMFMVVFAAIIFLFL 422
            +I  AI GGA  +L   Y+ + +F+V  A ++ L L
Sbjct: 38  -KITAAIHGGAMVYLNRQYRAIAVFVVFIAIVLALVL 73



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>HPPA_CLOTE (Q898Q9) Pyrophosphate-energized proton pump (EC 3.6.1.1)|
           (Pyrophosphate-energized inorganic pyrophosphatase)
           (H+-PPase) (Membrane-bound proton-translocating
           pyrophosphatase)
          Length = 673

 Score = 31.2 bits (69), Expect = 1.5
 Identities = 15/37 (40%), Positives = 23/37 (62%)
 Frame = +3

Query: 315 EIQTAISGGATSFLFTMYQYVGMFMVVFAAIIFLFLG 425
           EI   I  GA +FL T Y+Y+  F+V+   I+ +F+G
Sbjct: 38  EIAGHIHDGAMAFLKTEYKYLTGFIVIVTVILAIFVG 74



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>PRDM1_HUMAN (O75626) PR domain zinc finger protein 1 (PR domain-containing|
           protein 1) (Beta-interferon gene positive-regulatory
           domain I-binding factor) (BLIMP-1) (Positive regulatory
           domain I-binding factor 1) (PRDI-binding factor 1)
           (PRDI-BF1)
          Length = 789

 Score = 28.9 bits (63), Expect = 7.3
 Identities = 16/45 (35%), Positives = 25/45 (55%)
 Frame = -1

Query: 454 WPFVLNPSIDPRKRKIIAAKTTMNIPTYWYMVKRNDVAPPEMAVW 320
           W   +NP+  PR++ + A +  MNI  Y+Y +K    A  E+ VW
Sbjct: 122 WMRYVNPAHSPREQNLAACQNGMNI--YFYTIKPIP-ANQELLVW 163


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,668,112
Number of Sequences: 219361
Number of extensions: 568173
Number of successful extensions: 1985
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1935
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1979
length of database: 80,573,946
effective HSP length: 103
effective length of database: 57,979,763
effective search space used: 3246866728
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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