ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl01e05
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1WNK4_RAT (Q7TPK6) Serine/threonine-protein kinase WNK4 (EC 2.7.1... 35 0.065
2FEM2_CAEEL (P49594) Ca(2+)/calmodulin-dependent protein kinase p... 35 0.084
3WNK4_MOUSE (Q80UE6) Serine/threonine-protein kinase WNK4 (EC 2.7... 33 0.32
4EF1A_PIRIN (Q9HDF6) Elongation factor 1-alpha (EF-1-alpha) 33 0.32
5PP2C4_YEAST (P38089) Protein phosphatase 2C homolog 4 (EC 3.1.3.... 32 0.71
6CCMF_BRAJA (P45403) Cytochrome c-type biogenesis protein cycK 31 1.2
7ARGB_DEIRA (Q9RRP6) Acetylglutamate/acetylaminoadipate kinase (E... 31 1.6
8NRFA_PENUR (Q92269) Nitrogen regulatory protein nrfA 29 4.6
9GP179_HUMAN (Q6PRD1) Probable G-protein coupled receptor 179 pre... 28 7.9
10ITA7_HUMAN (Q13683) Integrin alpha-7 precursor [Contains: Integr... 28 7.9
11PPM1F_RAT (Q9WVR7) Ca(2+)/calmodulin-dependent protein kinase ph... 28 7.9
12PIGS_HUMAN (Q96S52) GPI transamidase component PIG-S (Phosphatid... 28 7.9

>WNK4_RAT (Q7TPK6) Serine/threonine-protein kinase WNK4 (EC 2.7.11.1)|
           (Protein kinase with no lysine 4) (Protein kinase,
           lysine-deficient 4)
          Length = 1222

 Score = 35.4 bits (80), Expect = 0.065
 Identities = 21/52 (40%), Positives = 26/52 (50%)
 Frame = +1

Query: 166 GGAPAVYQSAHCPRWTILSGGEGDGEKVSSSPQPRRCHVAAHRGRRRSQEDR 321
           G AP     + CP  T+ SGG G       S +P R   AA R RRR QE++
Sbjct: 110 GAAPVKAADSACPELTVSSGGPG-------SREPPRVPDAAARERRREQEEK 154



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>FEM2_CAEEL (P49594) Ca(2+)/calmodulin-dependent protein kinase phosphatase (EC|
           3.1.3.16) (CaM-kinase phosphatase) (CaMKPase)
           (Sex-determining protein fem-2) (Feminization of XX and
           XO animals protein 2)
          Length = 449

 Score = 35.0 bits (79), Expect = 0.084
 Identities = 19/51 (37%), Positives = 28/51 (54%)
 Frame = +1

Query: 292 RGRRRSQEDRAVCALGIRIPFLEQMRIKEVDVGVMAIFDGHNGDEASEMAS 444
           +G+R  QEDR +       P  + M   E  + V+A+FDGH G E S+ A+
Sbjct: 169 KGQRHKQEDRFLA-----YPNGQYMDRGEDPISVLAVFDGHGGHECSQYAA 214



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>WNK4_MOUSE (Q80UE6) Serine/threonine-protein kinase WNK4 (EC 2.7.11.1)|
           (Protein kinase with no lysine 4) (Protein kinase,
           lysine-deficient 4)
          Length = 1222

 Score = 33.1 bits (74), Expect = 0.32
 Identities = 36/113 (31%), Positives = 46/113 (40%), Gaps = 7/113 (6%)
 Frame = +1

Query: 4   RKPIILLPLLYSGDSPMSLQLSGRRTVLFLILIELLVPPRCAGESATCLAVYRE------ 165
           R+  + L LL S   P SL            L E   PP  AG + +  +  +E      
Sbjct: 61  RRSSVDLGLLSSWSQPASL------------LPEPPDPPDSAGPTRSPPSSSKEPPEGTW 108

Query: 166 -GGAPAVYQSAHCPRWTILSGGEGDGEKVSSSPQPRRCHVAAHRGRRRSQEDR 321
            G AP     + CP  T  SGG G       S +P R   AA R RRR QE++
Sbjct: 109 MGAAPVKAVDSACPELTGSSGGPG-------SREPPRVPDAAARERRREQEEK 154



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>EF1A_PIRIN (Q9HDF6) Elongation factor 1-alpha (EF-1-alpha)|
          Length = 462

 Score = 33.1 bits (74), Expect = 0.32
 Identities = 18/46 (39%), Positives = 23/46 (50%), Gaps = 1/46 (2%)
 Frame = +3

Query: 6   QAHNSSSPPLLRRLADVLTAIRPPYRPLSHPHRAPGAATVR-GGVG 140
           +   SSSP   + L D + AI PP RP   P R P     + GG+G
Sbjct: 215 EVKGSSSPATGKTLVDAIDAIEPPVRPSDKPLRLPLQDVYKIGGIG 260



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>PP2C4_YEAST (P38089) Protein phosphatase 2C homolog 4 (EC 3.1.3.16) (PP2C-4)|
          Length = 393

 Score = 32.0 bits (71), Expect = 0.71
 Identities = 19/62 (30%), Positives = 33/62 (53%), Gaps = 7/62 (11%)
 Frame = +1

Query: 280 VAAHRGRRRSQED-------RAVCALGIRIPFLEQMRIKEVDVGVMAIFDGHNGDEASEM 438
           V + +G R +QED        +V  +    PF++  + + + + V A+FDGH GD+ S+ 
Sbjct: 36  VGSMQGYRLTQEDAHLIRNENSVVYVRFFNPFID--KYETLSLNVFAVFDGHGGDDCSKF 93

Query: 439 AS 444
            S
Sbjct: 94  LS 95



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>CCMF_BRAJA (P45403) Cytochrome c-type biogenesis protein cycK|
          Length = 660

 Score = 31.2 bits (69), Expect = 1.2
 Identities = 21/51 (41%), Positives = 25/51 (49%), Gaps = 3/51 (5%)
 Frame = -1

Query: 261 RGGGDLLPIPLAAGEDGPAGAVRGLVHRG---RAALAVHGKARRRLPRAPW 118
           RGG  L P+ +  G    AGAV  LV R    R  LA   +  R LPR+ W
Sbjct: 444 RGGSALAPLAIGLGVFVIAGAVTDLVERTGLVRLPLATALQRARGLPRSAW 494



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>ARGB_DEIRA (Q9RRP6) Acetylglutamate/acetylaminoadipate kinase (EC 2.7.2.8) (EC|
           2.7.2.-) (NAG kinase) (AGK)
           (N-acetyl-L-glutamate/N-acetyl-L-aminoadipate
           5-phosphotransferase)
          Length = 249

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 26/74 (35%), Positives = 30/74 (40%), Gaps = 11/74 (14%)
 Frame = -1

Query: 255 GGDLLPIP--LAAGEDGPA---------GAVRGLVHRGRAALAVHGKARRRLPRAPWRHQ 109
           G  L P+   +A GEDG A         GAV G +  G   L       R  P    R  
Sbjct: 127 GAGLTPVVGCVAVGEDGEALNVNADTVAGAVAGALGEGAVFLTDVDGVYRAYPDPDSRAA 186

Query: 108 ELDEDEKEDGTAAG 67
           +L   E EDG AAG
Sbjct: 187 QLTRAEVEDGLAAG 200



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>NRFA_PENUR (Q92269) Nitrogen regulatory protein nrfA|
          Length = 865

 Score = 29.3 bits (64), Expect = 4.6
 Identities = 19/57 (33%), Positives = 28/57 (49%), Gaps = 5/57 (8%)
 Frame = -1

Query: 276 APPRLRGGGDL-----LPIPLAAGEDGPAGAVRGLVHRGRAALAVHGKARRRLPRAP 121
           +PP + G   L     +PI  A  + GP   V     +GRA + V  + +RRL +AP
Sbjct: 754 SPPSMTGSNTLGKPGVVPIAAAPPKSGPPAGVA----QGRAGVQVAPRRQRRLEKAP 806



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>GP179_HUMAN (Q6PRD1) Probable G-protein coupled receptor 179 precursor (Probable|
            G-protein coupled receptor 158-like 1)
          Length = 2367

 Score = 28.5 bits (62), Expect = 7.9
 Identities = 14/34 (41%), Positives = 18/34 (52%)
 Frame = -1

Query: 210  PAGAVRGLVHRGRAALAVHGKARRRLPRAPWRHQ 109
            P+ A    V     +  +H +ARRRLP  P RHQ
Sbjct: 903  PSPAKSSSVDSSHTSGRLHEEARRRLPHPPIRHQ 936



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>ITA7_HUMAN (Q13683) Integrin alpha-7 precursor [Contains: Integrin alpha-7 heavy|
            chain; Integrin alpha-7 light chain]
          Length = 1181

 Score = 28.5 bits (62), Expect = 7.9
 Identities = 14/38 (36%), Positives = 19/38 (50%)
 Frame = +1

Query: 202  PRWTILSGGEGDGEKVSSSPQPRRCHVAAHRGRRRSQE 315
            P    L GG+G G+K   SP+P   H+      RR +E
Sbjct: 922  PMQVELEGGQGPGQKGLCSPRPNILHLDVDSRDRRRRE 959



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>PPM1F_RAT (Q9WVR7) Ca(2+)/calmodulin-dependent protein kinase phosphatase (EC|
           3.1.3.16) (CaM-kinase phosphatase) (CaMKPase) (Partner
           of PIX 2) (Protein phosphatase 1F)
          Length = 450

 Score = 28.5 bits (62), Expect = 7.9
 Identities = 25/83 (30%), Positives = 36/83 (43%), Gaps = 4/83 (4%)
 Frame = +1

Query: 208 WTILSGGEGDGEKVSSSPQPRRCHVAAH--RGRRRSQEDRAVCALGIRIPFLEQM--RIK 375
           W + S  +      + +PQ R+  V+ H  R  RR  EDR V      +P    +     
Sbjct: 130 WEVCSQWQKRVPLTAQAPQ-RKWLVSIHAIRNTRRKMEDRHVS-----LPAFNHLFGLSD 183

Query: 376 EVDVGVMAIFDGHNGDEASEMAS 444
            V     A+FDGH G +A+  AS
Sbjct: 184 SVHRAYFAVFDGHGGVDAARYAS 206



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>PIGS_HUMAN (Q96S52) GPI transamidase component PIG-S|
           (Phosphatidylinositol-glycan biosynthesis, class S
           protein)
          Length = 554

 Score = 28.5 bits (62), Expect = 7.9
 Identities = 21/79 (26%), Positives = 33/79 (41%)
 Frame = +1

Query: 46  SPMSLQLSGRRTVLFLILIELLVPPRCAGESATCLAVYREGGAPAVYQSAHCPRWTILSG 225
           +P+  +L      L+ +L  LL  P  A      L +  + GAP    + H PRW  +  
Sbjct: 304 NPVESRLGSSAASLYPVLNFLLYVPELAHSP---LYIQDKDGAPVATNAFHSPRWGGIMV 360

Query: 226 GEGDGEKVSSSPQPRRCHV 282
              D +  ++S  P R  V
Sbjct: 361 YNVDSKTYNASVLPVRVEV 379


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,676,682
Number of Sequences: 219361
Number of extensions: 1009800
Number of successful extensions: 3700
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 3579
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3698
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2677159704
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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