ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bastl01e04
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1RFA1_SCHPO (Q92372) Replication factor-A protein 1 (Single-stran... 47 2e-05
2RFA1_HUMAN (P27694) Replication protein A 70 kDa DNA-binding sub... 45 6e-05
3RFA1_MOUSE (Q8VEE4) Replication protein A 70 kDa DNA-binding sub... 44 1e-04
4RFA1_PONPY (Q5R7Q4) Replication protein A 70 kDa DNA-binding sub... 43 3e-04
5RFA1_XENLA (Q01588) Replication protein A 70 kDa DNA-binding sub... 41 0.001
6RFA1_CHICK (Q5ZJJ2) Replication protein A 70 kDa DNA-binding sub... 39 0.005
7RFA1_XENTR (Q5FW17) Replication protein A 70 kDa DNA-binding sub... 38 0.013
8RFA1_BRARE (Q6NY74) Replication protein A 70 kDa DNA-binding sub... 35 0.086
9AK1C1_MACFU (Q95JH6) Aldo-keto reductase family 1 member C1 homo... 31 1.6
10AK1C1_MACFA (Q95JH7) Aldo-keto reductase family 1 member C1 homo... 31 1.6
11RFA1_DROME (Q24492) Replication protein A 70 kDa DNA-binding sub... 31 1.6
12ERA_MYCTU (P0A562) GTP-binding protein era homolog 31 1.6
13ERA_MYCBO (P0A563) GTP-binding protein era homolog 31 1.6
14MEP50_HUMAN (Q9BQA1) Methylosome protein 50 (MEP50 protein) (WD-... 30 3.6
15DADA_SHIFL (Q7UCT6) D-amino acid dehydrogenase small subunit (EC... 29 6.2
16PGFS2_BOVIN (P52897) Prostaglandin-F synthase 2 (EC 1.1.1.188) (... 29 6.2
17PGFS1_BOVIN (P05980) Prostaglandin-F synthase 1 (EC 1.1.1.188) (... 29 6.2
18ERA_MYCLE (Q49768) GTP-binding protein era homolog 29 6.2
19MEP50_MOUSE (Q99J09) Methylosome protein 50 (MEP50 protein) (WD-... 28 8.1
20STP2_MOUSE (P11378) Nuclear transition protein 2 (TP-2) (TP2) 28 8.1

>RFA1_SCHPO (Q92372) Replication factor-A protein 1 (Single-stranded|
           DNA-binding protein p68 subunit)
          Length = 609

 Score = 47.0 bits (110), Expect = 2e-05
 Identities = 25/74 (33%), Positives = 43/74 (58%)
 Frame = +3

Query: 228 PVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTLRVG 407
           P+LQV  ++ + +  T+         +R+R++LSD ++  QSML T LN L+ +  L+ G
Sbjct: 25  PILQVLTVKELNSNPTSGA------PKRYRVVLSDSINYAQSMLSTQLNHLVAENKLQKG 78

Query: 408 SIVHLTDMTCNTIQ 449
           + V LT  T N ++
Sbjct: 79  AFVQLTQFTVNVMK 92



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>RFA1_HUMAN (P27694) Replication protein A 70 kDa DNA-binding subunit (RP-A)|
           (RF-A) (Replication factor-A protein 1) (Single-stranded
           DNA-binding protein) (p70)
          Length = 616

 Score = 45.4 bits (106), Expect = 6e-05
 Identities = 28/95 (29%), Positives = 50/95 (52%), Gaps = 1/95 (1%)
 Frame = +3

Query: 168 LTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQ 347
           L+ GA+  I +   G    +P+LQV ++RP+   N+           R+R+L+SDG+++ 
Sbjct: 5   LSEGAIAAIMQ--KGDTNIKPILQVINIRPITTGNS---------PPRYRLLMSDGLNTL 53

Query: 348 QS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449
            S ML T LN L+++  L    +  +     NT++
Sbjct: 54  SSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLK 88



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>RFA1_MOUSE (Q8VEE4) Replication protein A 70 kDa DNA-binding subunit (RP-A)|
           (RF-A) (Replication factor-A protein 1) (p70)
          Length = 623

 Score = 44.3 bits (103), Expect = 1e-04
 Identities = 29/95 (30%), Positives = 50/95 (52%), Gaps = 1/95 (1%)
 Frame = +3

Query: 168 LTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQ 347
           L+ GA+  + +  N     +P+LQV ++RP+   N          S R+R+L+SDG+++ 
Sbjct: 5   LSEGAIEVMIQQEN--TSIKPILQVINIRPISTGN---------RSPRYRLLMSDGLNTL 53

Query: 348 QS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449
            S ML T LN L++ G L    +  +     NT++
Sbjct: 54  SSFMLATQLNTLVEGGQLASNCVCQVHKFIVNTLK 88



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>RFA1_PONPY (Q5R7Q4) Replication protein A 70 kDa DNA-binding subunit (RP-A)|
           (RF-A) (Replication factor-A protein 1) (p70)
          Length = 616

 Score = 43.1 bits (100), Expect = 3e-04
 Identities = 27/95 (28%), Positives = 48/95 (50%), Gaps = 1/95 (1%)
 Frame = +3

Query: 168 LTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQ 347
           L+ GA+  I +   G    +P+LQV ++RP+   N+           R+R+L+SDG+++ 
Sbjct: 5   LSEGAIAAIMQ--KGDTNIKPILQVINIRPITTGNS---------PPRYRLLMSDGLNTL 53

Query: 348 QS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449
            S M  T LN L++   L    +  +     NT++
Sbjct: 54  SSFMSATQLNPLVEQEQLSSNCVCQINRFIVNTLK 88



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>RFA1_XENLA (Q01588) Replication protein A 70 kDa DNA-binding subunit (RP-A)|
           (RF-A) (Replication factor-A protein 1) (Single-stranded
           DNA-binding protein) (p70)
          Length = 609

 Score = 41.2 bits (95), Expect = 0.001
 Identities = 29/99 (29%), Positives = 49/99 (49%), Gaps = 1/99 (1%)
 Frame = +3

Query: 156 APPPLTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDG 335
           A P L+ GA+  +     G +  +P LQV ++RP+   N            R+R+L+SDG
Sbjct: 2   ALPQLSEGAISAML---GGDSSCKPTLQVINIRPINTGNGP---------PRYRLLMSDG 49

Query: 336 VHSQQS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449
           +++  S ML T LN L+ +  L    I  ++    N ++
Sbjct: 50  LNTLSSFMLATQLNSLVDNNLLATNCICQVSRFIVNNLK 88



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>RFA1_CHICK (Q5ZJJ2) Replication protein A 70 kDa DNA-binding subunit (RP-A)|
           (RF-A) (Replication factor-A protein 1) (p70)
          Length = 614

 Score = 39.3 bits (90), Expect = 0.005
 Identities = 29/95 (30%), Positives = 48/95 (50%), Gaps = 1/95 (1%)
 Frame = +3

Query: 168 LTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQ 347
           L+ GA+  I +   G   ++PVLQV + R +   N            R+R+L+SDGV++ 
Sbjct: 5   LSEGAIAAIMQ---GENVYKPVLQVINTRAIATGNGP---------PRYRVLMSDGVNTL 52

Query: 348 QS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449
            S ML T LN L+++  L    I  +     N+++
Sbjct: 53  SSFMLATQLNPLVEEERLSAHCICQVNRFIVNSLK 87



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>RFA1_XENTR (Q5FW17) Replication protein A 70 kDa DNA-binding subunit (RP-A)|
           (RF-A) (Replication factor-A protein 1) (p70)
          Length = 609

 Score = 37.7 bits (86), Expect = 0.013
 Identities = 29/99 (29%), Positives = 48/99 (48%), Gaps = 1/99 (1%)
 Frame = +3

Query: 156 APPPLTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDG 335
           A P L+ GA+  I     G +  +P LQV ++R +   N            R+R+L+SDG
Sbjct: 2   ALPVLSEGAISAIL---GGDSSCKPTLQVINIRSINTGNGP---------PRYRLLMSDG 49

Query: 336 VHSQQS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449
           +++  S ML T LN L+ +  L    I  ++    N ++
Sbjct: 50  LNTLSSFMLATQLNFLVDNNLLATNCICQVSRFIVNNLK 88



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>RFA1_BRARE (Q6NY74) Replication protein A 70 kDa DNA-binding subunit (RP-A)|
           (RF-A) (Replication factor-A protein 1) (p70)
          Length = 601

 Score = 35.0 bits (79), Expect = 0.086
 Identities = 24/87 (27%), Positives = 38/87 (43%), Gaps = 1/87 (1%)
 Frame = +3

Query: 192 IWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQQS-MLGTG 368
           I  L  G     P+LQ  ++R +   N            RFR+++SDG+H+  S ML T 
Sbjct: 10  IESLSKGTEVNNPILQCVNIRKIDGGNGVS---------RFRVMMSDGLHTMSSFMLSTQ 60

Query: 369 LNDLIKDGTLRVGSIVHLTDMTCNTIQ 449
           LN + +   L    +  L     N ++
Sbjct: 61  LNPMAEQNQLATNCVCVLKRSVTNVLK 87



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>AK1C1_MACFU (Q95JH6) Aldo-keto reductase family 1 member C1 homolog (EC|
           1.-.-.-) (20-alpha-hydroxysteroid dehydrogenase) (EC
           1.1.1.149) (20-alpha-HSD) (Dihydrodiol dehydrogenase 1)
           (DD-1) (DD1)
          Length = 323

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 19/75 (25%), Positives = 32/75 (42%), Gaps = 1/75 (1%)
 Frame = +3

Query: 222 FQPVLQVADLRPV-VAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTL 398
           F PVL      P  V KN             FR + S  +++ +  +G  +   I DGT+
Sbjct: 15  FMPVLGFGTYAPAEVPKNKAIEATKLAIEAGFRHIDSAHLYNNEEYVGLAIRSKIADGTV 74

Query: 399 RVGSIVHLTDMTCNT 443
           +   I + + + CN+
Sbjct: 75  KREDIFYTSKLWCNS 89



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>AK1C1_MACFA (Q95JH7) Aldo-keto reductase family 1 member C1 homolog (EC|
           1.-.-.-) (20-alpha-hydroxysteroid dehydrogenase) (EC
           1.1.1.149) (20-alpha-HSD) (Dihydrodiol dehydrogenase 1)
           (DD-1) (DD1)
          Length = 323

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 19/75 (25%), Positives = 32/75 (42%), Gaps = 1/75 (1%)
 Frame = +3

Query: 222 FQPVLQVADLRPV-VAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTL 398
           F PVL      P  V KN             FR + S  +++ +  +G  +   I DGT+
Sbjct: 15  FMPVLGFGTYAPAEVPKNKALEATKLAIEAGFRHIDSAHLYNNEEYVGLAIRSKIADGTV 74

Query: 399 RVGSIVHLTDMTCNT 443
           +   I + + + CN+
Sbjct: 75  KREDIFYTSKLWCNS 89



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>RFA1_DROME (Q24492) Replication protein A 70 kDa DNA-binding subunit (RP-A)|
           (RF-A) (Replication factor-A protein 1) (Single-stranded
           DNA-binding protein) (DmRPA1) (p70)
          Length = 603

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 21/66 (31%), Positives = 35/66 (53%), Gaps = 1/66 (1%)
 Frame = +3

Query: 228 PVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVH-SQQSMLGTGLNDLIKDGTLRV 404
           PVLQ+  ++ +              SER+R+L+SDG + +  +ML + LN +  +G L  
Sbjct: 23  PVLQILAIKKI---------NSAADSERYRILISDGKYFNSYAMLASQLNVMQHNGELEE 73

Query: 405 GSIVHL 422
            +IV L
Sbjct: 74  FTIVQL 79



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>ERA_MYCTU (P0A562) GTP-binding protein era homolog|
          Length = 300

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 13/32 (40%), Positives = 24/32 (75%), Gaps = 2/32 (6%)
 Frame = +3

Query: 300 HSERFRMLLSD--GVHSQQSMLGTGLNDLIKD 389
           HS+ F+++L D  G+H  +++LG  LNDL+++
Sbjct: 50  HSDDFQIILVDTPGLHRPRTLLGKRLNDLVRE 81



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>ERA_MYCBO (P0A563) GTP-binding protein era homolog|
          Length = 300

 Score = 30.8 bits (68), Expect = 1.6
 Identities = 13/32 (40%), Positives = 24/32 (75%), Gaps = 2/32 (6%)
 Frame = +3

Query: 300 HSERFRMLLSD--GVHSQQSMLGTGLNDLIKD 389
           HS+ F+++L D  G+H  +++LG  LNDL+++
Sbjct: 50  HSDDFQIILVDTPGLHRPRTLLGKRLNDLVRE 81



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>MEP50_HUMAN (Q9BQA1) Methylosome protein 50 (MEP50 protein) (WD-repeat protein|
           77)
          Length = 342

 Score = 29.6 bits (65), Expect = 3.6
 Identities = 17/35 (48%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +3

Query: 153 EAPPPLTPGAVMEIWEL-PNGPARFQPVLQVADLR 254
           E PPPL P A  E W L PN PA  +  L+ A  R
Sbjct: 4   ETPPPLVPPAARE-WNLPPNAPACMERQLEAARYR 37



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>DADA_SHIFL (Q7UCT6) D-amino acid dehydrogenase small subunit (EC 1.4.99.1)|
          Length = 432

 Score = 28.9 bits (63), Expect = 6.2
 Identities = 21/65 (32%), Positives = 29/65 (44%), Gaps = 1/65 (1%)
 Frame = +3

Query: 219 RFQPVLQVADLRPVVAKNTTXXXXXXQHSERF-RMLLSDGVHSQQSMLGTGLNDLIKDGT 395
           RF   ++V  +  +V  NT       +  E   R L   G H +Q+   TGL  +  DGT
Sbjct: 303 RFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGT 362

Query: 396 LRVGS 410
             VGS
Sbjct: 363 PVVGS 367



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>PGFS2_BOVIN (P52897) Prostaglandin-F synthase 2 (EC 1.1.1.188) (PGF synthase 2)|
           (PGF 2) (Prostaglandin-D2 11 reductase 2) (PGFSII)
          Length = 323

 Score = 28.9 bits (63), Expect = 6.2
 Identities = 19/77 (24%), Positives = 33/77 (42%), Gaps = 1/77 (1%)
 Frame = +3

Query: 222 FQPVLQVADLRPV-VAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTL 398
           F PVL      P  V K+             FR + S  ++  +  +G  +   I DGT+
Sbjct: 15  FIPVLGFGTYAPEEVPKSEALEATKFAIEVGFRHVDSAHLYQNEEQVGQAIRSKIADGTV 74

Query: 399 RVGSIVHLTDMTCNTIQ 449
           +   I + + + CN++Q
Sbjct: 75  KREDIFYTSKLWCNSLQ 91



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>PGFS1_BOVIN (P05980) Prostaglandin-F synthase 1 (EC 1.1.1.188) (PGF synthase 1)|
           (PGF 1) (Prostaglandin-D2 11 reductase 1) (PGFSI)
          Length = 323

 Score = 28.9 bits (63), Expect = 6.2
 Identities = 19/77 (24%), Positives = 33/77 (42%), Gaps = 1/77 (1%)
 Frame = +3

Query: 222 FQPVLQVADLRPV-VAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTL 398
           F PVL      P  V K+             FR + S  ++  +  +G  +   I DGT+
Sbjct: 15  FIPVLGFGTYAPEEVPKSEALEATKFAIEVGFRHVDSAHLYQNEEQVGQAIRSKIADGTV 74

Query: 399 RVGSIVHLTDMTCNTIQ 449
           +   I + + + CN++Q
Sbjct: 75  KREDIFYTSKLWCNSLQ 91



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>ERA_MYCLE (Q49768) GTP-binding protein era homolog|
          Length = 300

 Score = 28.9 bits (63), Expect = 6.2
 Identities = 11/50 (22%), Positives = 28/50 (56%)
 Frame = +3

Query: 240 VADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKD 389
           +  ++P   ++T       + + +  ++ + G+H  +++LG  LNDL++D
Sbjct: 33  ITSMKPQTTRHTIRGIVHREGNFQIVLVDTPGLHRPRTLLGKRLNDLVRD 82



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>MEP50_MOUSE (Q99J09) Methylosome protein 50 (MEP50 protein) (WD-repeat protein|
           77)
          Length = 342

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 16/35 (45%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
 Frame = +3

Query: 153 EAPPPLTPGAVMEIWEL-PNGPARFQPVLQVADLR 254
           + PPPL P A  E W L PN PA  +  L+ A  R
Sbjct: 4   DTPPPLVPPAARE-WNLPPNAPACMERQLEAARYR 37



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>STP2_MOUSE (P11378) Nuclear transition protein 2 (TP-2) (TP2)|
          Length = 117

 Score = 28.5 bits (62), Expect = 8.1
 Identities = 11/20 (55%), Positives = 15/20 (75%), Gaps = 1/20 (5%)
 Frame = +2

Query: 332 RRPLPAVHARHRPQRP-HKG 388
           + P P+VH+RH P RP H+G
Sbjct: 60  KHPKPSVHSRHSPARPSHRG 79


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.314    0.138    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,642,897
Number of Sequences: 219361
Number of extensions: 543231
Number of successful extensions: 1389
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1369
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1385
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2735358828
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
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