Clone Name | bastl01e04 |
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Clone Library Name | barley_pub |
>RFA1_SCHPO (Q92372) Replication factor-A protein 1 (Single-stranded| DNA-binding protein p68 subunit) Length = 609 Score = 47.0 bits (110), Expect = 2e-05 Identities = 25/74 (33%), Positives = 43/74 (58%) Frame = +3 Query: 228 PVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTLRVG 407 P+LQV ++ + + T+ +R+R++LSD ++ QSML T LN L+ + L+ G Sbjct: 25 PILQVLTVKELNSNPTSGA------PKRYRVVLSDSINYAQSMLSTQLNHLVAENKLQKG 78 Query: 408 SIVHLTDMTCNTIQ 449 + V LT T N ++ Sbjct: 79 AFVQLTQFTVNVMK 92
>RFA1_HUMAN (P27694) Replication protein A 70 kDa DNA-binding subunit (RP-A)| (RF-A) (Replication factor-A protein 1) (Single-stranded DNA-binding protein) (p70) Length = 616 Score = 45.4 bits (106), Expect = 6e-05 Identities = 28/95 (29%), Positives = 50/95 (52%), Gaps = 1/95 (1%) Frame = +3 Query: 168 LTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQ 347 L+ GA+ I + G +P+LQV ++RP+ N+ R+R+L+SDG+++ Sbjct: 5 LSEGAIAAIMQ--KGDTNIKPILQVINIRPITTGNS---------PPRYRLLMSDGLNTL 53 Query: 348 QS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449 S ML T LN L+++ L + + NT++ Sbjct: 54 SSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLK 88
>RFA1_MOUSE (Q8VEE4) Replication protein A 70 kDa DNA-binding subunit (RP-A)| (RF-A) (Replication factor-A protein 1) (p70) Length = 623 Score = 44.3 bits (103), Expect = 1e-04 Identities = 29/95 (30%), Positives = 50/95 (52%), Gaps = 1/95 (1%) Frame = +3 Query: 168 LTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQ 347 L+ GA+ + + N +P+LQV ++RP+ N S R+R+L+SDG+++ Sbjct: 5 LSEGAIEVMIQQEN--TSIKPILQVINIRPISTGN---------RSPRYRLLMSDGLNTL 53 Query: 348 QS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449 S ML T LN L++ G L + + NT++ Sbjct: 54 SSFMLATQLNTLVEGGQLASNCVCQVHKFIVNTLK 88
>RFA1_PONPY (Q5R7Q4) Replication protein A 70 kDa DNA-binding subunit (RP-A)| (RF-A) (Replication factor-A protein 1) (p70) Length = 616 Score = 43.1 bits (100), Expect = 3e-04 Identities = 27/95 (28%), Positives = 48/95 (50%), Gaps = 1/95 (1%) Frame = +3 Query: 168 LTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQ 347 L+ GA+ I + G +P+LQV ++RP+ N+ R+R+L+SDG+++ Sbjct: 5 LSEGAIAAIMQ--KGDTNIKPILQVINIRPITTGNS---------PPRYRLLMSDGLNTL 53 Query: 348 QS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449 S M T LN L++ L + + NT++ Sbjct: 54 SSFMSATQLNPLVEQEQLSSNCVCQINRFIVNTLK 88
>RFA1_XENLA (Q01588) Replication protein A 70 kDa DNA-binding subunit (RP-A)| (RF-A) (Replication factor-A protein 1) (Single-stranded DNA-binding protein) (p70) Length = 609 Score = 41.2 bits (95), Expect = 0.001 Identities = 29/99 (29%), Positives = 49/99 (49%), Gaps = 1/99 (1%) Frame = +3 Query: 156 APPPLTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDG 335 A P L+ GA+ + G + +P LQV ++RP+ N R+R+L+SDG Sbjct: 2 ALPQLSEGAISAML---GGDSSCKPTLQVINIRPINTGNGP---------PRYRLLMSDG 49 Query: 336 VHSQQS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449 +++ S ML T LN L+ + L I ++ N ++ Sbjct: 50 LNTLSSFMLATQLNSLVDNNLLATNCICQVSRFIVNNLK 88
>RFA1_CHICK (Q5ZJJ2) Replication protein A 70 kDa DNA-binding subunit (RP-A)| (RF-A) (Replication factor-A protein 1) (p70) Length = 614 Score = 39.3 bits (90), Expect = 0.005 Identities = 29/95 (30%), Positives = 48/95 (50%), Gaps = 1/95 (1%) Frame = +3 Query: 168 LTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQ 347 L+ GA+ I + G ++PVLQV + R + N R+R+L+SDGV++ Sbjct: 5 LSEGAIAAIMQ---GENVYKPVLQVINTRAIATGNGP---------PRYRVLMSDGVNTL 52 Query: 348 QS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449 S ML T LN L+++ L I + N+++ Sbjct: 53 SSFMLATQLNPLVEEERLSAHCICQVNRFIVNSLK 87
>RFA1_XENTR (Q5FW17) Replication protein A 70 kDa DNA-binding subunit (RP-A)| (RF-A) (Replication factor-A protein 1) (p70) Length = 609 Score = 37.7 bits (86), Expect = 0.013 Identities = 29/99 (29%), Positives = 48/99 (48%), Gaps = 1/99 (1%) Frame = +3 Query: 156 APPPLTPGAVMEIWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDG 335 A P L+ GA+ I G + +P LQV ++R + N R+R+L+SDG Sbjct: 2 ALPVLSEGAISAIL---GGDSSCKPTLQVINIRSINTGNGP---------PRYRLLMSDG 49 Query: 336 VHSQQS-MLGTGLNDLIKDGTLRVGSIVHLTDMTCNTIQ 449 +++ S ML T LN L+ + L I ++ N ++ Sbjct: 50 LNTLSSFMLATQLNFLVDNNLLATNCICQVSRFIVNNLK 88
>RFA1_BRARE (Q6NY74) Replication protein A 70 kDa DNA-binding subunit (RP-A)| (RF-A) (Replication factor-A protein 1) (p70) Length = 601 Score = 35.0 bits (79), Expect = 0.086 Identities = 24/87 (27%), Positives = 38/87 (43%), Gaps = 1/87 (1%) Frame = +3 Query: 192 IWELPNGPARFQPVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQQS-MLGTG 368 I L G P+LQ ++R + N RFR+++SDG+H+ S ML T Sbjct: 10 IESLSKGTEVNNPILQCVNIRKIDGGNGVS---------RFRVMMSDGLHTMSSFMLSTQ 60 Query: 369 LNDLIKDGTLRVGSIVHLTDMTCNTIQ 449 LN + + L + L N ++ Sbjct: 61 LNPMAEQNQLATNCVCVLKRSVTNVLK 87
>AK1C1_MACFU (Q95JH6) Aldo-keto reductase family 1 member C1 homolog (EC| 1.-.-.-) (20-alpha-hydroxysteroid dehydrogenase) (EC 1.1.1.149) (20-alpha-HSD) (Dihydrodiol dehydrogenase 1) (DD-1) (DD1) Length = 323 Score = 30.8 bits (68), Expect = 1.6 Identities = 19/75 (25%), Positives = 32/75 (42%), Gaps = 1/75 (1%) Frame = +3 Query: 222 FQPVLQVADLRPV-VAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTL 398 F PVL P V KN FR + S +++ + +G + I DGT+ Sbjct: 15 FMPVLGFGTYAPAEVPKNKAIEATKLAIEAGFRHIDSAHLYNNEEYVGLAIRSKIADGTV 74 Query: 399 RVGSIVHLTDMTCNT 443 + I + + + CN+ Sbjct: 75 KREDIFYTSKLWCNS 89
>AK1C1_MACFA (Q95JH7) Aldo-keto reductase family 1 member C1 homolog (EC| 1.-.-.-) (20-alpha-hydroxysteroid dehydrogenase) (EC 1.1.1.149) (20-alpha-HSD) (Dihydrodiol dehydrogenase 1) (DD-1) (DD1) Length = 323 Score = 30.8 bits (68), Expect = 1.6 Identities = 19/75 (25%), Positives = 32/75 (42%), Gaps = 1/75 (1%) Frame = +3 Query: 222 FQPVLQVADLRPV-VAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTL 398 F PVL P V KN FR + S +++ + +G + I DGT+ Sbjct: 15 FMPVLGFGTYAPAEVPKNKALEATKLAIEAGFRHIDSAHLYNNEEYVGLAIRSKIADGTV 74 Query: 399 RVGSIVHLTDMTCNT 443 + I + + + CN+ Sbjct: 75 KREDIFYTSKLWCNS 89
>RFA1_DROME (Q24492) Replication protein A 70 kDa DNA-binding subunit (RP-A)| (RF-A) (Replication factor-A protein 1) (Single-stranded DNA-binding protein) (DmRPA1) (p70) Length = 603 Score = 30.8 bits (68), Expect = 1.6 Identities = 21/66 (31%), Positives = 35/66 (53%), Gaps = 1/66 (1%) Frame = +3 Query: 228 PVLQVADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVH-SQQSMLGTGLNDLIKDGTLRV 404 PVLQ+ ++ + SER+R+L+SDG + + +ML + LN + +G L Sbjct: 23 PVLQILAIKKI---------NSAADSERYRILISDGKYFNSYAMLASQLNVMQHNGELEE 73 Query: 405 GSIVHL 422 +IV L Sbjct: 74 FTIVQL 79
>ERA_MYCTU (P0A562) GTP-binding protein era homolog| Length = 300 Score = 30.8 bits (68), Expect = 1.6 Identities = 13/32 (40%), Positives = 24/32 (75%), Gaps = 2/32 (6%) Frame = +3 Query: 300 HSERFRMLLSD--GVHSQQSMLGTGLNDLIKD 389 HS+ F+++L D G+H +++LG LNDL+++ Sbjct: 50 HSDDFQIILVDTPGLHRPRTLLGKRLNDLVRE 81
>ERA_MYCBO (P0A563) GTP-binding protein era homolog| Length = 300 Score = 30.8 bits (68), Expect = 1.6 Identities = 13/32 (40%), Positives = 24/32 (75%), Gaps = 2/32 (6%) Frame = +3 Query: 300 HSERFRMLLSD--GVHSQQSMLGTGLNDLIKD 389 HS+ F+++L D G+H +++LG LNDL+++ Sbjct: 50 HSDDFQIILVDTPGLHRPRTLLGKRLNDLVRE 81
>MEP50_HUMAN (Q9BQA1) Methylosome protein 50 (MEP50 protein) (WD-repeat protein| 77) Length = 342 Score = 29.6 bits (65), Expect = 3.6 Identities = 17/35 (48%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +3 Query: 153 EAPPPLTPGAVMEIWEL-PNGPARFQPVLQVADLR 254 E PPPL P A E W L PN PA + L+ A R Sbjct: 4 ETPPPLVPPAARE-WNLPPNAPACMERQLEAARYR 37
>DADA_SHIFL (Q7UCT6) D-amino acid dehydrogenase small subunit (EC 1.4.99.1)| Length = 432 Score = 28.9 bits (63), Expect = 6.2 Identities = 21/65 (32%), Positives = 29/65 (44%), Gaps = 1/65 (1%) Frame = +3 Query: 219 RFQPVLQVADLRPVVAKNTTXXXXXXQHSERF-RMLLSDGVHSQQSMLGTGLNDLIKDGT 395 RF ++V + +V NT + E R L G H +Q+ TGL + DGT Sbjct: 303 RFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPDGT 362 Query: 396 LRVGS 410 VGS Sbjct: 363 PVVGS 367
>PGFS2_BOVIN (P52897) Prostaglandin-F synthase 2 (EC 1.1.1.188) (PGF synthase 2)| (PGF 2) (Prostaglandin-D2 11 reductase 2) (PGFSII) Length = 323 Score = 28.9 bits (63), Expect = 6.2 Identities = 19/77 (24%), Positives = 33/77 (42%), Gaps = 1/77 (1%) Frame = +3 Query: 222 FQPVLQVADLRPV-VAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTL 398 F PVL P V K+ FR + S ++ + +G + I DGT+ Sbjct: 15 FIPVLGFGTYAPEEVPKSEALEATKFAIEVGFRHVDSAHLYQNEEQVGQAIRSKIADGTV 74 Query: 399 RVGSIVHLTDMTCNTIQ 449 + I + + + CN++Q Sbjct: 75 KREDIFYTSKLWCNSLQ 91
>PGFS1_BOVIN (P05980) Prostaglandin-F synthase 1 (EC 1.1.1.188) (PGF synthase 1)| (PGF 1) (Prostaglandin-D2 11 reductase 1) (PGFSI) Length = 323 Score = 28.9 bits (63), Expect = 6.2 Identities = 19/77 (24%), Positives = 33/77 (42%), Gaps = 1/77 (1%) Frame = +3 Query: 222 FQPVLQVADLRPV-VAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKDGTL 398 F PVL P V K+ FR + S ++ + +G + I DGT+ Sbjct: 15 FIPVLGFGTYAPEEVPKSEALEATKFAIEVGFRHVDSAHLYQNEEQVGQAIRSKIADGTV 74 Query: 399 RVGSIVHLTDMTCNTIQ 449 + I + + + CN++Q Sbjct: 75 KREDIFYTSKLWCNSLQ 91
>ERA_MYCLE (Q49768) GTP-binding protein era homolog| Length = 300 Score = 28.9 bits (63), Expect = 6.2 Identities = 11/50 (22%), Positives = 28/50 (56%) Frame = +3 Query: 240 VADLRPVVAKNTTXXXXXXQHSERFRMLLSDGVHSQQSMLGTGLNDLIKD 389 + ++P ++T + + + ++ + G+H +++LG LNDL++D Sbjct: 33 ITSMKPQTTRHTIRGIVHREGNFQIVLVDTPGLHRPRTLLGKRLNDLVRD 82
>MEP50_MOUSE (Q99J09) Methylosome protein 50 (MEP50 protein) (WD-repeat protein| 77) Length = 342 Score = 28.5 bits (62), Expect = 8.1 Identities = 16/35 (45%), Positives = 19/35 (54%), Gaps = 1/35 (2%) Frame = +3 Query: 153 EAPPPLTPGAVMEIWEL-PNGPARFQPVLQVADLR 254 + PPPL P A E W L PN PA + L+ A R Sbjct: 4 DTPPPLVPPAARE-WNLPPNAPACMERQLEAARYR 37
>STP2_MOUSE (P11378) Nuclear transition protein 2 (TP-2) (TP2)| Length = 117 Score = 28.5 bits (62), Expect = 8.1 Identities = 11/20 (55%), Positives = 15/20 (75%), Gaps = 1/20 (5%) Frame = +2 Query: 332 RRPLPAVHARHRPQRP-HKG 388 + P P+VH+RH P RP H+G Sbjct: 60 KHPKPSVHSRHSPARPSHRG 79 Database: uniprot_sprot.fasta Posted date: May 25, 2006 5:36 PM Number of letters in database: 80,573,946 Number of sequences in database: 219,361 Lambda K H 0.314 0.138 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 36,642,897 Number of Sequences: 219361 Number of extensions: 543231 Number of successful extensions: 1389 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 1369 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1385 length of database: 80,573,946 effective HSP length: 102 effective length of database: 58,199,124 effective search space used: 2735358828 frameshift window, decay const: 50, 0.1 T: 12 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits)