ナショナルバイオリソースプロジェクト
-Barley Genetic Resources Database-
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更新日:2016年5月1日

Clone Information

BLAST Search Result

Clone Name bart58g10
Clone Library Name barley_pub

No. Definition Score
(bits)
E
Value
1SPD1_NEPCL (P19837) Spidroin 1 (Dragline silk fibroin 1) (Fragment) 33 0.34
2PG54_MYCTU (O53553) Hypothetical PE-PGRS family protein PE_PGRS5... 29 4.9
3SYK_CERSY (O74059) Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--t... 28 8.4

>SPD1_NEPCL (P19837) Spidroin 1 (Dragline silk fibroin 1) (Fragment)|
          Length = 747

 Score = 33.1 bits (74), Expect = 0.34
 Identities = 23/69 (33%), Positives = 27/69 (39%), Gaps = 2/69 (2%)
 Frame = -3

Query: 453 GRGGDGAPVSAV*NISGAD--VQXXXXXXXXXXXXXXPAYGVGGLVGQIDGEGDAAVGGA 280
           GRGG GA  +A   +      ++                 G GGL GQ  G   AA GGA
Sbjct: 487 GRGGQGAGAAAAAAVGAGQEGIRGQGAGQGGYGGLGSQGSGRGGLGGQGAGAAAAAAGGA 546

Query: 279 QWGGCGSVG 253
             GG G  G
Sbjct: 547 GQGGLGGQG 555



 Score = 32.7 bits (73), Expect = 0.45
 Identities = 26/71 (36%), Positives = 29/71 (40%)
 Frame = -3

Query: 453 GRGGDGAPVSAV*NISGADVQXXXXXXXXXXXXXXPAYGVGGLVGQIDGEGDAAVGGAQW 274
           GRGG GA  +A     GA                    G GGL GQ  G   AA GGA  
Sbjct: 132 GRGGQGAAAAAA---GGAG-------QGGYGGLGSQGAGRGGLGGQGAGAAAAAAGGAGQ 181

Query: 273 GGCGSVGEEDA 241
           GG G +G + A
Sbjct: 182 GGYGGLGGQGA 192



 Score = 31.6 bits (70), Expect = 0.99
 Identities = 23/75 (30%), Positives = 27/75 (36%), Gaps = 4/75 (5%)
 Frame = -3

Query: 453 GRGGDGAPVSAV*NISGADVQXXXXXXXXXXXXXXPAYGVGGLVGQIDGEG----DAAVG 286
           G GG GA       +                       G GGL GQ  G+G     AA G
Sbjct: 287 GLGGQGAGQGGYGGLGSQGAGRGGLGGQGAGAAAAGGAGQGGLGGQGAGQGAGAAAAAAG 346

Query: 285 GAQWGGCGSVGEEDA 241
           GA  GG G +G + A
Sbjct: 347 GAGQGGYGGLGSQGA 361



 Score = 31.2 bits (69), Expect = 1.3
 Identities = 23/78 (29%), Positives = 26/78 (33%), Gaps = 7/78 (8%)
 Frame = -3

Query: 453 GRGGDGAPVSAV*NISGADVQXXXXXXXXXXXXXXPAYGVGGLVGQIDGEGD-------A 295
           G GG GA       + G                     G GGL  Q  G G        A
Sbjct: 20  GLGGQGAGQGGYGGLGGQGAGQGAGAAAAAAAGGAGQGGYGGLGSQGAGRGGQGAGAAAA 79

Query: 294 AVGGAQWGGCGSVGEEDA 241
           A GGA  GG G +G + A
Sbjct: 80  AAGGAGQGGYGGLGSQGA 97



 Score = 30.8 bits (68), Expect = 1.7
 Identities = 26/75 (34%), Positives = 30/75 (40%), Gaps = 4/75 (5%)
 Frame = -3

Query: 453 GRGGDGAPVSAV*NISGADVQXXXXXXXXXXXXXXPAYGVGGLVGQIDGEG----DAAVG 286
           G GG GA  +A     GA                    G GGL GQ  G+G     AA G
Sbjct: 209 GLGGQGAGAAAAAAAGGA--------------------GQGGLGGQGAGQGAGASAAAAG 248

Query: 285 GAQWGGCGSVGEEDA 241
           GA  GG G +G + A
Sbjct: 249 GAGQGGYGGLGSQGA 263



 Score = 30.0 bits (66), Expect = 2.9
 Identities = 17/37 (45%), Positives = 21/37 (56%), Gaps = 4/37 (10%)
 Frame = -3

Query: 339 GVGGLVGQIDGEG----DAAVGGAQWGGCGSVGEEDA 241
           G GGL GQ  G+G     AA GG + GG G +G + A
Sbjct: 547 GQGGLGGQGAGQGAGAAAAAAGGVRQGGYGGLGSQGA 583



 Score = 28.5 bits (62), Expect = 8.4
 Identities = 27/75 (36%), Positives = 29/75 (38%), Gaps = 8/75 (10%)
 Frame = -3

Query: 453 GRGGDGAPVSAV*NISGADVQXXXXXXXXXXXXXXPAYGVGGLVGQ--------IDGEGD 298
           GRGG GA  +A    +G   Q                 G GGL GQ          G G 
Sbjct: 584 GRGGQGAGAAAA--AAGGAGQG----------------GYGGLGGQGVGRGGLGGQGAGA 625

Query: 297 AAVGGAQWGGCGSVG 253
           AA GGA  GG G VG
Sbjct: 626 AAAGGAGQGGYGGVG 640



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>PG54_MYCTU (O53553) Hypothetical PE-PGRS family protein PE_PGRS54 precursor|
          Length = 1901

 Score = 29.3 bits (64), Expect = 4.9
 Identities = 22/67 (32%), Positives = 24/67 (35%)
 Frame = -3

Query: 453  GRGGDGAPVSAV*NISGADVQXXXXXXXXXXXXXXPAYGVGGLVGQIDGEGDAAVGGAQW 274
            G GGDGA    +  +SG D                   G GG  G     GD A G A  
Sbjct: 1002 GDGGDGASGLGL-GLSGFDGGQGGQGGAGGSAGAGGINGAGGAGGNGGDGGDGATGAAGL 1060

Query: 273  GGCGSVG 253
            G  G VG
Sbjct: 1061 GDNGGVG 1067



 Score = 29.3 bits (64), Expect = 4.9
 Identities = 22/67 (32%), Positives = 24/67 (35%)
 Frame = -3

Query: 453 GRGGDGAPVSAV*NISGADVQXXXXXXXXXXXXXXPAYGVGGLVGQIDGEGDAAVGGAQW 274
           G GGDGA    +  +SG D                   G GG  G     GD A G A  
Sbjct: 801 GDGGDGASGLGL-GLSGFDGGQGGQGGAGGSAGAGGINGAGGAGGNGGDGGDGATGAAGL 859

Query: 273 GGCGSVG 253
           G  G VG
Sbjct: 860 GDNGGVG 866



 Score = 28.5 bits (62), Expect = 8.4
 Identities = 14/31 (45%), Positives = 17/31 (54%)
 Frame = -3

Query: 345 AYGVGGLVGQIDGEGDAAVGGAQWGGCGSVG 253
           A G GG  G +   G+A +GG   GG G VG
Sbjct: 657 AIGTGGTGGAVGSVGNAGIGGT--GGTGGVG 685



 Score = 28.5 bits (62), Expect = 8.4
 Identities = 22/73 (30%), Positives = 27/73 (36%), Gaps = 4/73 (5%)
 Frame = -3

Query: 453 GRGGDGAPVSAV*NISGADVQXXXXXXXXXXXXXXPAYGVGGLVGQIDGEGDAAVGGAQW 274
           G GG GA  ++     GAD                 A G GG  G +   G A +GG   
Sbjct: 451 GMGGSGADNASG---IGADGGAGGTGGNAGAGGAGGAAGTGGTGGVVGAAGKAGIGGTGG 507

Query: 273 ----GGCGSVGEE 247
               GG GS G +
Sbjct: 508 QGGAGGAGSAGTD 520



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>SYK_CERSY (O74059) Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA ligase)|
           (LysRS)
          Length = 524

 Score = 28.5 bits (62), Expect = 8.4
 Identities = 12/20 (60%), Positives = 13/20 (65%)
 Frame = -3

Query: 303 GDAAVGGAQWGGCGSVGEED 244
           GDA VGG +  GCG  GE D
Sbjct: 208 GDAEVGGRKIAGCGHEGEAD 227


  Database: uniprot_sprot.fasta
    Posted date:  May 25, 2006  5:36 PM
  Number of letters in database: 80,573,946
  Number of sequences in database:  219,361
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,660,743
Number of Sequences: 219361
Number of extensions: 514106
Number of successful extensions: 2777
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 2493
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2749
length of database: 80,573,946
effective HSP length: 102
effective length of database: 58,199,124
effective search space used: 2851757076
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
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